Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,888,053	C→A	intergenic (‑62/+8)	rnd ← / ← fadD	RNase D/fatty acyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,888,053	0	C	A	100.0%	151.0 / NA	43	intergenic (‑62/+8)	rnd/fadD	RNase D/fatty acyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (20/23);  total (20/23)

TATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACTTTGTATCCCGTGAGCT  >  NC_000913/1887917‑1888188                                                                                                                                         |                                                                                                                                        tATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGAATTa                                                                                                                                      >  2:346551/1‑140 (MQ=255) tATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGAATTa                                                                                                                                      >  2:362265/1‑140 (MQ=255) tATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGAATTa                                                                                                                                      >  2:1073927/1‑140 (MQ=255) tATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGAATTa                                                                                                                                      >  2:662231/1‑140 (MQ=255)     gCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGAATTAACGc                                                                                                                                  <  2:1258505/140‑1 (MQ=255)     gCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGAATTAACGc                                                                                                                                  <  1:662231/140‑1 (MQ=255)            aGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGAATTAACGCTCAGGCt                                                                                                                           <  2:20184/140‑1 (MQ=255)                        cTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGAATTAACGCTCAGGCTTTATTGTCCACt                                                                                                               >  1:511106/1‑140 (MQ=255)                                aaaGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGAATTAACGCTCAGGCTTTATTGTCCACTTTGCcgcg                                                                                                       <  1:693321/140‑1 (MQ=255)                                       ccAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGAATTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCg                                                                                                <  1:1073927/140‑1 (MQ=255)                                       ccAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGAATTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCg                                                                                                <  2:444470/140‑1 (MQ=255)                                        cAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGAATTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGt                                                                                               >  2:544141/1‑140 (MQ=255)                                             cATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGAATTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGt                                                                                          <  2:127008/140‑1 (MQ=255)                                                        gTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGAATTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCtcgtcg                                                                               >  1:1222611/1‑140 (MQ=255)                                                                ttGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGAATTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAAttt                                                                       >  2:734170/1‑140 (MQ=255)                                                                ttGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGAATTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAAttt                                                                       >  1:450440/1‑140 (MQ=255)                                                                ttGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGAATTAACGCTCAGGCTTTATTCTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAAttt                                                                       >  1:835148/1‑140 (MQ=255)                                                                  ggTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGAATTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAAttttt                                                                     >  1:1249483/1‑140 (MQ=255)                                                                   gTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGAATTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTc                                                                    <  2:228790/140‑1 (MQ=255)                                                                    tAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGAATTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTcc                                                                   <  2:1249483/140‑1 (MQ=255)                                                                    tAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGAATTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTcc                                                                   <  2:1231768/140‑1 (MQ=255)                                                                       ttCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGAATTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGAc                                                                >  1:375112/1‑140 (MQ=255)                                                                           aaTTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGAATTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTa                                                            <  1:544141/140‑1 (MQ=255)                                                                             ttGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGAATTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTc                                                                    <  2:669869/130‑1 (MQ=255)                                                                             ttGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGAATTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTc                                                                    >  1:1135898/1‑130 (MQ=255)                                                                             ttGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGAATTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTc                                                                    <  2:1135898/130‑1 (MQ=255)                                                                             ttGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGAATTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTc                                                                    >  1:669869/1‑130 (MQ=255)                                                                              tGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGAATTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGAt                                                         <  1:362265/140‑1 (MQ=255)                                                                                  ttCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGAATTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCg                                                     <  1:346551/140‑1 (MQ=255)                                                                                      ctTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGAATTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTaa                                                 <  2:511106/140‑1 (MQ=255)                                                                                              ggCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGAATTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGAt                                                         <  1:477978/124‑1 (MQ=255)                                                                                              ggCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGAATTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGAt                                                         >  2:477978/1‑124 (MQ=255)                                                                                              ggCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGAATTAACGCTCAGGCTTTATTCTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCAc                                         <  2:835148/140‑1 (MQ=255)                                                                                               gCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGAATTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACg                                        >  1:616384/1‑140 (MQ=255)                                                                                                 gTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGAATTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGaa                                      >  2:300498/1‑140 (MQ=255)                                                                                                   cAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGAATTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAAc                                    >  2:496701/1‑140 (MQ=255)                                                                                                         aaaCGCCGGATTAACCGGCGTCTGACGACTGAATTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTccacc                              >  2:105954/1‑140 (MQ=255)                                                                                                         aaaCGCCGGATTAACCGGCGTCTGACGACTGAATTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTccacc                              <  1:1100742/140‑1 (MQ=255)                                                                                                              ccGGATTAACCGGCGTCTGACGACTGAATTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCtt                         <  2:1222611/140‑1 (MQ=255)                                                                                                              ccGGATTAACCGGCGTCTGACGACTGAATTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCtt                         <  1:496701/140‑1 (MQ=255)                                                                                                                  aTTAACCGGCGTCTGACGACTGAATTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGt                     <  2:1132483/140‑1 (MQ=255)                                                                                                                  aTTAACCGGCGTCTGACGACTGAATTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGt                     <  1:711713/140‑1 (MQ=255)                                                                                                                                 acgacTGAATTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACTTTGTATCCCGTg      >  2:759274/1‑140 (MQ=255)                                                                                                                                   gactgaatTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACTTTGTATCCCGTGAg    <  1:105954/140‑1 (MQ=255)                                                                                                                                     ctgaatTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACTTTGTATCCCGTGAGCt  >  2:1201497/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        TATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACTTTGTATCCCGTGAGCT  >  NC_000913/1887917‑1888188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑