Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,227,228	C→A	G15C (GGC→TGC)	yohF ←	putative oxidoreductase with NAD(P)‑binding Rossmann‑fold domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,227,228	0	C	A	100.0%	221.8 / NA	63	G15C (GGC→TGC)	yohF	putative oxidoreductase with NAD(P)‑binding Rossmann‑fold domain
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (29/34);  total (29/34)

ACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTT  >  NC_000913/2227101‑2227360                                                                                                                                |                                                                                                                                     aCGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCAGATCCCCGAATc                                                                                                                          >  2:836407/1‑140 (MQ=255)   gATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCAGATCCCCGAATCgg                                                                                                                        <  2:257712/140‑1 (MQ=255)      ctcCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCAGATCCCCGAATCGGAgg                                                                                                                     <  2:372720/140‑1 (MQ=255)          gCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTACATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGt                                                                                                                 <  2:302664/140‑1 (MQ=255)           cACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGta                                                                                                                >  1:103777/1‑140 (MQ=255)           cACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGta                                                                                                                >  2:358839/1‑140 (MQ=255)            aCGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGtaa                                                                                                               <  2:293905/140‑1 (MQ=255)               tACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGtaataa                                                                                                            >  2:38746/1‑140 (MQ=255)               tACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGtaataa                                                                                                            >  2:1004847/1‑140 (MQ=255)                        gCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCt                                                                                                   >  2:378412/1‑140 (MQ=255)                        gCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCt                                                                                                   <  1:836229/140‑1 (MQ=255)                         cTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACTtg                                                                                                  >  2:307605/1‑140 (MQ=255)                         cTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCtg                                                                                                  <  1:378412/140‑1 (MQ=255)                         cTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCtg                                                                                                  <  1:432251/140‑1 (MQ=255)                           aaCTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCtgtg                                                                                                <  2:103777/140‑1 (MQ=255)                              tACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCa                                                                                             <  1:307605/140‑1 (MQ=255)                                ccTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATc                                                                                           <  1:1132539/140‑1 (MQ=255)                                  tCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGcc                                                                                              <  2:873125/135‑1 (MQ=255)                                  tCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGcc                                                                                              >  1:873125/1‑135 (MQ=255)                                  tCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGa                                                                                         <  1:274904/140‑1 (MQ=255)                                       cgcgGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTtct                                                                                    <  1:836407/140‑1 (MQ=255)                                        gcgGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTtctc                                                                                   >  2:953448/1‑140 (MQ=255)                                          ggTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCa                                                                                 >  1:1196091/1‑140 (MQ=255)                                          ggTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCa                                                                                 >  2:680973/1‑140 (MQ=255)                                                    gCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTg                                                                       >  2:556404/1‑140 (MQ=255)                                                      cccTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGaa                                                                     >  1:1140606/1‑140 (MQ=255)                                                          tcttcATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACt                                                                               >  2:917411/1‑126 (MQ=255)                                                          tcttcATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACt                                                                               <  1:917411/126‑1 (MQ=255)                                                            ttcATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACg                                                               <  1:953448/140‑1 (MQ=255)                                                             tcATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGt                                                              <  1:1004847/140‑1 (MQ=255)                                                              cATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGtt                                                             <  2:881331/140‑1 (MQ=255)                                                               aTCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTa                                                            <  1:38746/140‑1 (MQ=255)                                                                    aGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGtat                                                       >  2:617104/1‑140 (MQ=255)                                                                    aGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGtat                                                       >  1:1270590/1‑140 (MQ=255)                                                                        ccAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATaa                                                                  >  1:956333/1‑125 (MQ=255)                                                                        ccAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATaa                                                                  <  2:956333/125‑1 (MQ=255)                                                                        ccAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATaa                                                                  >  1:204223/1‑125 (MQ=255)                                                                        ccAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATaa                                                                  <  2:204223/125‑1 (MQ=255)                                                                          aGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAgg                                                 >  2:463087/1‑140 (MQ=255)                                                                              aaTACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCa                                             <  2:1066883/140‑1 (MQ=255)                                                                                     atatCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATatcatc                                      <  1:358839/140‑1 (MQ=255)                                                                                       atCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGaa                                                   >  1:6978/1‑125 (MQ=255)                                                                                       atCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGaa                                                   <  2:6978/125‑1 (MQ=255)                                                                                            aCCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATatcatc                                      <  2:842243/133‑1 (MQ=255)                                                                                            aCCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATatcatc                                      >  1:842243/1‑133 (MQ=255)                                                                                                 tgctgcGCCAGTAATAACGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTAcc                          >  2:984252/1‑140 (MQ=255)                                                                                                 tgctgcGCCAGTAATAACGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTAcc                          >  2:1237605/1‑140 (MQ=255)                                                                                                       gcCAGTAATAACGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTACCCCCCGc                    >  2:490492/1‑140 (MQ=255)                                                                                                       gcCAGTAATAACGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTACCCCCCGc                    >  2:89559/1‑140 (MQ=255)                                                                                                        ccAGTAATAACGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTACCCCCCGcc                   <  1:978878/140‑1 (MQ=255)                                                                                                        ccAGTAATAACGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTACCCCCCGcc                   >  1:1204856/1‑140 (MQ=255)                                                                                                           gTAATAACGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTACCCCCCGCCCaa                <  2:1270590/140‑1 (MQ=255)                                                                                                            taataaCGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTACCCCCCGCCCAAc               <  1:369124/140‑1 (MQ=255)                                                                                                             aataaCGCGCACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTACCCCCCGCCCAACg              <  1:617104/140‑1 (MQ=255)                                                                                                                  cgcgCACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCa         <  1:984252/140‑1 (MQ=255)                                                                                                                   gcgcACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCa            >  1:1125818/1‑136 (MQ=255)                                                                                                                   gcgcACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCa            <  2:1125818/136‑1 (MQ=255)                                                                                                                   gcgcACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAg        <  1:680973/140‑1 (MQ=255)                                                                                                                    cgcACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGt       <  1:1237605/140‑1 (MQ=255)                                                                                                                    cgcACTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGt       >  1:755440/1‑140 (MQ=255)                                                                                                                       aCTCTTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAg    <  2:1140606/140‑1 (MQ=255)                                                                                                                         tctTTGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATACAAGGCGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGtt  >  1:1055709/1‑140 (MQ=255)                                                                                                                             tGCAGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTACCCCCCGCCCAACg              <  1:455916/124‑1 (MQ=255)                                                                                                                                |                                                                                                                                     ACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTT  >  NC_000913/2227101‑2227360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑