Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,564,480	T→C	intergenic (+108/‑43)	yffR → / → yffS	CPZ‑55 prophage; uncharacterized protein YffR/CPZ‑55 prophage; uncharacterized protein YffS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,564,480	0	T	C	100.0%	270.5 / NA	69	intergenic (+108/‑43)	yffR/yffS	CPZ‑55 prophage; uncharacterized protein YffR/CPZ‑55 prophage; uncharacterized protein YffS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/32);  total (37/32)

TAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCGAG  >  NC_000913/2564344‑2564612                                                                                                                                         |                                                                                                                                     taggtagAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGtt                                                                                                                                   <  2:733883/140‑1 (MQ=255)     tagAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAAcc                                                                                                                               <  2:482656/140‑1 (MQ=255)       gagTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTc                                                                                                                             <  1:668491/140‑1 (MQ=255)        agTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTcc                                                                                                                            >  2:817335/1‑140 (MQ=255)           aTTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCaa                                                                                                                         >  1:707269/1‑140 (MQ=255)           aTTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCaa                                                                                                                         >  2:664347/1‑140 (MQ=255)           aTTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCaa                                                                                                                         <  2:607714/140‑1 (MQ=255)           aTTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCaa                                                                                                                         <  1:1253709/140‑1 (MQ=255)              tGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCAATgg                                                                                                                      >  2:655628/1‑140 (MQ=255)               gAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCAATGGa                                                                                                                     <  1:919223/140‑1 (MQ=255)               gAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCAATGGa                                                                                                                     >  2:885405/1‑140 (MQ=255)               gAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCAATGGa                                                                                                                     >  1:510807/1‑140 (MQ=255)                  aaaCGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCAATGGATTc                                                                                                                  >  1:950560/1‑140 (MQ=255)                   aaCGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCAATGGATTCt                                                                                                                 >  1:12030/1‑140 (MQ=255)                    aCGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCa                                                                                                                          >  2:785619/1‑130 (MQ=255)                    aCGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCa                                                                                                                          <  1:785619/130‑1 (MQ=255)                             attgCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCAATGGATTCTATGAGATggg                                                                                                       >  1:42236/1‑140 (MQ=255)                               tgCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGgag                                                                                                     >  1:1230926/1‑140 (MQ=255)                               tgCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGgag                                                                                                     >  2:634929/1‑140 (MQ=255)                                     tAGCAACAACTAAACAACCAATACACCCGGCCCCTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGGAGATaaaa                                                                                               >  1:1274551/1‑140 (MQ=255)                                        caacaaCTAAACAACCAATACACCCGGCCCCTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATc                                                                                            >  2:1020501/1‑140 (MQ=255)                                        caacaaCTAAACAACCAATACACCCGGCCCCTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATc                                                                                            >  2:1088680/1‑140 (MQ=255)                                        caacaaCTAAACAACCAATACACCCGGCCCCTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATc                                                                                            >  1:1134404/1‑140 (MQ=255)                                                      ccAATACACCCGGCCCCTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAAt                                                                              >  1:550121/1‑140 (MQ=255)                                                         aTACACCCGGCCCCTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGATATACGAAATCaa                                                                           >  2:1129296/1‑140 (MQ=255)                                                                cGGCCCCTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGc                                                                    >  2:49293/1‑140 (MQ=255)                                                                cGGCCCCTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGc                                                                    >  1:121187/1‑140 (MQ=255)                                                                 ggCCCCTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCg                                                                   <  2:1274551/140‑1 (MQ=255)                                                                 ggCCCCTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCg                                                                   >  2:673449/1‑140 (MQ=255)                                                                 ggCCCCTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCg                                                                   >  1:360191/1‑140 (MQ=255)                                                                  gCCCCTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAAt                                                                        >  2:1120484/1‑134 (MQ=255)                                                                  gCCCCTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAAt                                                                        <  1:1120484/134‑1 (MQ=255)                                                                  gCCCCTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGa                                                                  <  2:88417/140‑1 (MQ=255)                                                                   ccccTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGAc                                                                 <  1:885405/140‑1 (MQ=255)                                                                    cccTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACa                                                                <  1:778883/140‑1 (MQ=255)                                                                    cccTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACa                                                                >  2:831716/1‑140 (MQ=255)                                                                           cGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCaa                                                         <  1:1211768/140‑1 (MQ=255)                                                                                    tgtgATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGc                                                <  2:950560/140‑1 (MQ=255)                                                                                    tgtgATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGc                                                >  2:979759/1‑140 (MQ=255)                                                                                        aTCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATg                                            >  2:491351/1‑140 (MQ=255)                                                                                         tCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATgg                                           <  1:979759/140‑1 (MQ=255)                                                                                            gCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAg                                        <  1:1020501/140‑1 (MQ=255)                                                                                            gCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAg                                        <  1:1088680/140‑1 (MQ=255)                                                                                            gCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAg                                        <  2:861261/140‑1 (MQ=255)                                                                                            gCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAg                                        <  2:1230926/140‑1 (MQ=255)                                                                                              gTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGa                                      <  1:673449/140‑1 (MQ=255)                                                                                              gTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGa                                      <  1:49293/140‑1 (MQ=255)                                                                                              gTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGa                                      <  2:707269/140‑1 (MQ=255)                                                                                              gTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGa                                      <  2:42236/140‑1 (MQ=255)                                                                                              gTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGa                                      <  2:510807/140‑1 (MQ=255)                                                                                                      tCCCTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATaa                              <  2:1207635/140‑1 (MQ=255)                                                                                                       cccTTCCGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAg                             <  1:491351/140‑1 (MQ=255)                                                                                                            ccGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGa                                      >  1:438050/1‑126 (MQ=255)                                                                                                            ccGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGa                                      <  2:438050/126‑1 (MQ=255)                                                                                                            ccGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGgcgc                        >  2:622162/1‑140 (MQ=255)                                                                                                             cGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAg                                        <  1:838829/123‑1 (MQ=255)                                                                                                             cGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAg                                        >  2:838829/1‑123 (MQ=255)                                                                                                             cGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAggg                           <  1:721017/136‑1 (MQ=255)                                                                                                             cGTTACGCTTACCCTCCACCAATACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAggg                           >  2:721017/1‑136 (MQ=255)                                                                                                                  cGCTTACCCTCCACCAATACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACg                  >  2:892029/1‑140 (MQ=255)                                                                                                                       aCCCTCCACCAATACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTgggg             >  1:269667/1‑140 (MQ=255)                                                                                                                       aCCCTCCACCAATACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTgggg             >  2:645570/1‑140 (MQ=255)                                                                                                                             caccaATACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCt                       <  1:557591/124‑1 (MQ=255)                                                                                                                             caccaATACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCt                       >  2:557591/1‑124 (MQ=255)                                                                                                                             caccaATACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAAt       >  2:203369/1‑140 (MQ=255)                                                                                                                              accaATACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATg      >  1:27639/1‑140 (MQ=255)                                                                                                                                caATACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCg    <  1:645570/140‑1 (MQ=255)                                                                                                                                  aTACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCgag  <  2:269667/140‑1 (MQ=255)                                                                                                                                  aTACGCGCGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCgag  >  1:928519/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                     TAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCTCGCCGGGTTTTTTGTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCGAG  >  NC_000913/2564344‑2564612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑