Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,011,002	C→T	intergenic (+2/‑74)	yigL → / → bioP	phosphosugar phosphatase YigL/biotin transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,011,002	0	C	T	100.0%	165.3 / NA	47	intergenic (+2/‑74)	yigL/bioP	phosphosugar phosphatase YigL/biotin transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/32);  total (15/32)

TCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGGCGCTACTTATCATCACCACGATTCTGTGGGCCTTCTCCTTTAGCTTTTATGGCGAGTAC  >  NC_000913/4010866‑4011138                                                                                                                                         |                                                                                                                                         tCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGtt                                                                                                                                       >  1:51037/1‑140 (MQ=255)      ggCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTa                                                                                                                                  >  2:363437/1‑140 (MQ=255)            gAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGt                                                                                                                            <  2:117707/140‑1 (MQ=255)             aaaGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTc                                                                                                                           <  1:916375/140‑1 (MQ=255)             aaaGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTc                                                                                                                           <  2:687961/140‑1 (MQ=255)             aaaGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTc                                                                                                                           <  2:1186752/140‑1 (MQ=255)               aGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAg                                                                                                                         <  1:1223502/140‑1 (MQ=255)                     cATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGt                                                                                                                            >  2:609598/1‑131 (MQ=255)                     cATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGt                                                                                                                            <  1:609598/131‑1 (MQ=255)                          tGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTg                                                                                                              >  1:820324/1‑140 (MQ=255)                                 tGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTc                                                                                                       >  1:975762/1‑140 (MQ=255)                                  gcgcACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGt                                                                                                                     <  2:77825/125‑1 (MQ=255)                                  gcgcACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGt                                                                                                                     >  1:77825/1‑125 (MQ=255)                                  gcgcACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCg                                                                                                      <  1:1018712/140‑1 (MQ=255)                                   cgcACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGc                                                                                                     <  2:33178/140‑1 (MQ=255)                                    gcACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCt                                                                                                    <  2:551316/140‑1 (MQ=255)                                     cACCAGCGTCTGAAAGACCTTCATCCCTAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTa                                                                                                   <  1:25872/140‑1 (MQ=255)                                     cACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTa                                                                                                   <  1:390222/140‑1 (MQ=255)                                     cACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTa                                                                                                   <  2:820324/140‑1 (MQ=255)                                           cGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTa                                                                                                   <  1:795742/134‑1 (MQ=255)                                           cGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTa                                                                                                   >  2:795742/1‑134 (MQ=255)                                            gTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGa                                                                                            <  2:1013448/140‑1 (MQ=255)                                             tCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGAt                                                                                           <  1:606955/140‑1 (MQ=255)                                                    gACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGtt                                                                                    <  1:363437/140‑1 (MQ=255)                                                     aCCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTa                                                                                   >  2:258853/1‑140 (MQ=255)                                                      ccTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTaa                                                                                  >  2:1227985/1‑140 (MQ=255)                                                           aTCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCaaa                                                                             <  2:975762/140‑1 (MQ=255)                                                               cGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGt                                                                         >  2:1013268/1‑140 (MQ=255)                                                                        aGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATtg                                                                <  1:1227985/140‑1 (MQ=255)                                                                        aGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTCTCCATtg                                                                <  1:1013268/140‑1 (MQ=255)                                                                           gATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTgg                                                             <  1:757649/140‑1 (MQ=255)                                                                            aTTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGgc                                                            >  2:301383/1‑140 (MQ=255)                                                                             ttGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATAATTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGgcg                                                           <  1:254106/140‑1 (MQ=255)                                                                              tGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGgcgc                                                          <  2:1046472/140‑1 (MQ=255)                                                                              tGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGgcgc                                                          <  1:301383/140‑1 (MQ=255)                                                                                      aTGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGGCGCTa                                                        >  2:394592/1‑134 (MQ=255)                                                                                      aTGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGGCGCTa                                                        <  1:394592/134‑1 (MQ=255)                                                                                       tGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGGCGCTACTTatca                                                 >  2:905206/1‑140 (MQ=255)                                                                                           gacgacGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGGCGCTACTTATCATcac                                             <  1:572868/140‑1 (MQ=255)                                                                                             cgacgCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGGCGCTACTTATCATcacca                                           <  2:51037/140‑1 (MQ=255)                                                                                               acgCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGGCGCTACTTATCATCACCACg                                         <  1:714521/140‑1 (MQ=255)                                                                                                cgcgGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGGCGCTACTTATCATCACCACGa                                        <  1:905206/140‑1 (MQ=255)                                                                                                  cgGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGGCGCTACTTATCATCACCACGAtt                                      >  2:1846/1‑140 (MQ=255)                                                                                                  cgGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGGCGCTACTTATCATCACCACGAtt                                      <  1:811241/140‑1 (MQ=255)                                                                                                  cgGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGGCGCTACTTATCATCACCACGAtt                                      <  1:1182147/140‑1 (MQ=255)                                                                                                                              tttATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGGCGCTACTTATCATCACCACGATTCTGTGGGCCTTCTCCTTTAGCTTTTATg          <  1:399544/140‑1 (MQ=255)                                                                                                                                      aaTTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGGCGCTACTTATCATCACCACGATTCTGTGGGCCTTCTCCTTTAGCTTTTATGGCGAGTAc  >  1:1208979/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                         TCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGGCGCTACTTATCATCACCACGATTCTGTGGGCCTTCTCCTTTAGCTTTTATGGCGAGTAC  >  NC_000913/4010866‑4011138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑