Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,233,447	T→G	intergenic (‑214/‑311)	lysC ← / → pgi	aspartate kinase III/glucose‑6‑phosphate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,233,447	0	T	G	100.0%	324.1 / NA	82	intergenic (‑214/‑311)	lysC/pgi	aspartate kinase III/glucose‑6‑phosphate isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (32/50);  total (32/50)

TTCGCTACTATTTTCACCCAGAAGTGCTCCACCACTTGCGAAACGCCCGACTGCGAACGCTTCTGGTGACAACCCAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGATGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGG  >  NC_000913/4233312‑4233580                                                                                                                                        |                                                                                                                                      ttCGCTACTATTTTCACCCAGAAGTGCTCCACCACTTGCGAAACGCCCGACTGCGAACGCTTCTGGTGACAACCCAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCAc                                                                                                                                   <  1:568981/140‑1 (MQ=255) ttCGCTACTATTTTCACCCAGAAGTGCTCCACCACTTGCGAAACGCCCGACTGCGAACGCTTCTGGTGACAACCCAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCAc                                                                                                                                   <  1:551047/140‑1 (MQ=255)   cGCTACTATTTTCACCCAGAAGTGCTCCACCACTTGCGAAACGCCCGACTGCGAACGCTTCTGGTGACAACCCAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCAccc                                                                                                                                 >  2:279522/1‑140 (MQ=255)   cGCTACTATTTTCACCCAGAAGTGCTCCACCACTTGCGAAACGCCCGACTGCGAACGCTTCTGGTGACAACCCAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCAccc                                                                                                                                 >  2:1222318/1‑140 (MQ=255)        ctaTTTTCACCCAGAAGTGCTCCACCACTTGCGAAACGCCCGACTGCGAACGCTTCTGGTGACAACCCAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCa                                                                                                                            <  2:443338/140‑1 (MQ=255)        ctaTTTTCACCCAGAAGTGCTCCACCACTTGCGAAACGCCCGACTGCGAACGCTTCTGGTGACAACCCAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCa                                                                                                                            <  1:1222318/140‑1 (MQ=255)        ctaTTTTCACCCAGAAGTGCTCCACCACTTGCGAAACGCCCGACTGCGAACGCTTCTGGTGACAACCCAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCa                                                                                                                            >  1:350008/1‑140 (MQ=255)         taTTTTCACCCAGAAGTGCTCCACCACTTGCGAAACGCCCGACTGCGAACGCTTCTGGTGACAACCCAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAg                                                                                                                           <  1:889830/140‑1 (MQ=255)           ttttCACCCAGAAGTGCTCCACCACTTGCGAAACGCCCGACTGCGAACGCTTCTGGTGACAACCCAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGgt                                                                                                                         <  2:74626/140‑1 (MQ=255)           ttttCACCCAGAAGTGCTCCACCACTTGCGAAACGCCCGACTGCGAACGCTTCTGGTGACAACCCAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGgt                                                                                                                         <  1:734724/140‑1 (MQ=255)             ttCACCCAGAAGTGCTCCACCACTTGCGAAACGCCCGACTGCGAACGCTTCTGGTGACAACCCAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCTATCACCTCGGCGAGGCACCCCCTCAGgtgt                                                                                                                       >  1:502762/1‑140 (MQ=255)             ttCACCCAGAAGTGCTCCACCACTTGCGAAACGCCCGACTGCGAACGCTTCTGGTGACAACCCAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGgtgt                                                                                                                       <  1:778710/140‑1 (MQ=255)                     gAAGTGCTCCACCACTTGCGAAACGCCCGACTGCGAACGCTTCTGGTGACAACCCAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGgt                                                                                                                         >  1:812401/1‑130 (MQ=255)                     gAAGTGCTCCACCACTTGCGAAACGCCCGACTGCGAACGCTTCTGGTGACAACCCAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGgt                                                                                                                         <  2:812401/130‑1 (MQ=255)                     gAAGTGCTCCACCACTTGCGAAACGCCCGACTGCGAACGCTTCTGGTGACAACCCAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAg                                                                                                               >  2:959174/1‑140 (MQ=255)                       aGTGCTCCACCACTTGCGAAACGCCCGACTGCGAACGCTTCTGGTGACAACCCAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGa                                                                                                             <  1:941936/140‑1 (MQ=255)                            tCCACCACTTGCGAAACGCCCGACTGCGAACGCTTCTGGTGACAACCCAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGc                                                                                                        <  1:959174/140‑1 (MQ=255)                            tCCACCACTTGCGAAACGCCCGACTGCGAACGCTTCTGGTGACAACCCAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGc                                                                                                        <  1:1140152/140‑1 (MQ=255)                              caccacTTGCGAAACGCCTGACTGCGAACGCTTCTGGTGACAACCCAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGctc                                                                                                      <  2:294907/140‑1 (MQ=255)                              caccacTTGCGAAACGCCCGACTGCGAACGCTTCTGGTGACAACCCAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGctc                                                                                                      >  2:507409/1‑140 (MQ=255)                                ccacTTGCGAAACGCCCGACTGCGAACGCTTCTGGTGACAACCCAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGctcct                                                                                                    >  2:1191164/1‑140 (MQ=255)                                ccacTTGCGAAACGCCCGACTGCGAACGCTTCTGGTGACAACCCAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGctcct                                                                                                    >  1:147/1‑140 (MQ=255)                                  acTTGCGAAACGCCCGACTGCGAACGCTTCTGGTGACAACCCAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCtcctcc                                                                                                  <  1:852970/140‑1 (MQ=255)                                   cTTGCGAAACGCCCGACTGCGAACGCTTCTGGTGACAACCCAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGc                                                                                                        >  2:1190019/1‑133 (MQ=255)                                   cTTGCGAAACGCCCGACTGCGAACGCTTCTGGTGACAACCCAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGc                                                                                                        <  1:1190019/133‑1 (MQ=255)                                   cTTGCGAAACGCCCGACTGCGAACGCTTCTGGTGACAACCCAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCa                                                                                                 >  1:658923/1‑140 (MQ=255)                                    ttGCGAAACGCCCGACTGCGAACGCTTCTGGTGACAACCCAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCACTCAGGTGTTATCACAGGACTGGCTCCTCCaa                                                                                                <  2:54042/140‑1 (MQ=255)                                     tGCGAAACGCCCGACTGCGAACGCTTCTGGTGACAACCCAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCtcctcc                                                                                                  >  2:883660/1‑137 (MQ=255)                                     tGCGAAACGCCCGACTGCGAACGCTTCTGGTGACAACCCAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCtcctcc                                                                                                  <  1:883660/137‑1 (MQ=255)                                       cGAAACGCCCGACTGCGAACGCTTCTGGTGACAACCCAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAacac                                                                                             >  1:1121389/1‑140 (MQ=255)                                        gAAACGCCCGACTGCGAACGCTTCTGGTGACAACCCAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACAcc                                                                                            >  1:928119/1‑140 (MQ=255)                                          aaCGCCCGACTGCGAACGCTTCTGGTGACAACCCAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGt                                                                                          <  2:931845/140‑1 (MQ=255)                                           aCGCCCGACTGCGAACGCTTCTGGTGACAACCCAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGtt                                                                                         >  1:78031/1‑140 (MQ=255)                                              cccGACTGCGAACGCTTCTGGTGACAACCCAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGc                                                                                                        <  1:1042809/122‑1 (MQ=255)                                              cccGACTGCGAACGCTTCTGGTGACAACCCAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGc                                                                                                        >  2:1042809/1‑122 (MQ=255)                                              cccGACTGCGAACGCTTCTGGTGACAACCCAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGTTACt                                                                                      >  2:1145761/1‑140 (MQ=255)                                              cccGACTGCGAACGCTTCTGGTGACAACCCAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGTTACt                                                                                      >  1:991972/1‑140 (MQ=255)                                               ccGACTGCGAACGCTTCTGGTGACAACCCAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGTTACtt                                                                                     <  2:928119/140‑1 (MQ=255)                                                cGACTGCGAACGCTTCTGGTGACAACCCAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGTTACTTg                                                                                    <  1:701571/140‑1 (MQ=255)                                                     gCGAACGCTTCTGGTGACAACCCAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGTTACTTGGGCaa                                                                               <  2:658923/140‑1 (MQ=255)                                                          cGCTTCTGGTGACAACCCAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCTATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGTTACTTGGGCAACgcgc                                                                          <  2:502762/140‑1 (MQ=255)                                                          cGCTTCTGGTGACAACCCAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGTTACTTGGGCAACgcgc                                                                          <  2:60680/140‑1 (MQ=255)                                                          cGCTTCTGGTGACAACCCAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGTTACTTGGGCAACgcgc                                                                          <  2:731657/140‑1 (MQ=255)                                                                    gACAACCCAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGc                                                                <  2:1085450/140‑1 (MQ=255)                                                                    gACAACCCAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGc                                                                <  2:70750/140‑1 (MQ=255)                                                                     aCAACCCAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTgggc                                                               <  1:660925/140‑3 (MQ=255)                                                                     aCAACCCAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGcc                                                               >  1:529699/1‑140 (MQ=255)                                                                       aaCCCAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTg                                                             >  1:295335/1‑140 (MQ=255)                                                                         cccAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTgcg                                                           <  2:295335/140‑1 (MQ=255)                                                                         cccAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTgcg                                                           <  2:529699/140‑1 (MQ=255)                                                                         cccAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTgcg                                                           <  2:78031/140‑1 (MQ=255)                                                                         cccAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTgcg                                                           <  2:609355/140‑1 (MQ=255)                                                                         cccAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTgcg                                                           <  1:1191164/140‑1 (MQ=255)                                                                         cccAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTgcg                                                           <  2:63987/140‑1 (MQ=255)                                                                         cccAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTgcg                                                           >  2:309218/1‑140 (MQ=255)                                                                         cccAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTgcg                                                           <  2:641768/140‑1 (MQ=255)                                                                           cAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCt                                                         <  1:279522/140‑1 (MQ=255)                                                                           cAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCt                                                         <  1:287121/140‑1 (MQ=255)                                                                            aGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCt                                                         >  2:869237/1‑139 (MQ=255)                                                                            aGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCt                                                         <  1:869237/139‑1 (MQ=255)                                                                              gggATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAgc                                                      <  2:350008/140‑1 (MQ=255)                                                                              gggATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAgc                                                      >  1:841160/1‑140 (MQ=255)                                                                                gATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAgcgc                                                    <  1:507409/140‑1 (MQ=255)                                                                                   tCAGCCCCTGTAGCCGATGATGAACGTGGTCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAgg                                                 >  2:1173474/1‑140 (MQ=255)                                                                                   tCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAgg                                                 >  2:553830/1‑140 (MQ=255)                                                                                              aGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACATTta                                      <  2:991972/140‑1 (MQ=255)                                                                                              aGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACATTta                                      <  2:1121272/140‑1 (MQ=255)                                                                                              aGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACATTta                                      <  1:771198/140‑1 (MQ=255)                                                                                              aGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACATTta                                      <  1:1145761/140‑1 (MQ=255)                                                                                                    tgatgaACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACATTTataaata                                <  2:500078/140‑1 (MQ=255)                                                                                                    tgatgaACGTGGCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACATTTataaata                                >  2:410909/1‑140 (MQ=255)                                                                                                              ggCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGc                      >  2:779837/1‑140 (MQ=255)                                                                                                               gCCAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCg                     >  2:144575/1‑140 (MQ=255)                                                                                                                ccAGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCgg                    >  1:845366/1‑140 (MQ=255)                                                                                                                  aGCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCgggg                  <  1:1173474/140‑1 (MQ=255)                                                                                                                   gCCGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGc                 >  2:119812/1‑140 (MQ=255)                                                                                                                    ccGTTCAATCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCg                >  1:99372/1‑140 (MQ=255)                                                                                                                          aaTCACCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGTGGTTGTc          <  1:31550/140‑1 (MQ=255)                                                                                                                              aCCTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACg      <  1:144575/140‑1 (MQ=255)                                                                                                                               ccTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGa     <  1:779837/140‑1 (MQ=255)                                                                                                                               ccTCGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGa     <  1:553830/140‑1 (MQ=255)                                                                                                                                  cGGCGAGGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATgg  >  1:289279/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                      TTCGCTACTATTTTCACCCAGAAGTGCTCCACCACTTGCGAAACGCCCGACTGCGAACGCTTCTGGTGACAACCCAGGGGATTCAGCCCCTGTAGCCGATGATGAACGTGGCCAGCCGTTCAATCACCTCGGCGATGCACCCCCTCAGGTGTTATCACAGGACTGGCTCCTCCAACACCGTTACTTGGGCAACGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGG  >  NC_000913/4233312‑4233580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑