Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,267,926	A→T	T203S (ACC→TCC)	ychA →	transglutaminase‑like/TPR repeat‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,267,926	0	A	T	100.0%	278.7 / NA	78	T203S (ACC→TCC)	ychA	transglutaminase‑like/TPR repeat‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (41/37);  total (41/37)

AAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGAAGCTGATAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCACCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑GCGCAACTGGATTGCGAACACGTTGCGTTGAACGATTTAAGTTATTTCGTTGAACAGTGTCCGGAAG  >  NC_000913/1267793‑1268061                                                                                                                                      |                                                                                                                                          aaaTATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGAAGCTGATAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCg                                                                                                                                     <  1:655027/140‑1 (MQ=255) aaaTATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGAAGCTGATAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCg                                                                                                                                     >  2:655027/1‑140 (MQ=255)  aaTATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGAAGCTGATAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGa                                                                                                                                    >  2:407287/1‑140 (MQ=255)       cAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGAAGCTGATAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCtt                                                                                                                               <  2:730509/140‑1 (MQ=255)              tCGGCGGAACTGTTTTATGAAGACCTTGATGAAGCTGATAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACa                                                                                                                        >  1:597198/1‑140 (MQ=255)                  cggAACTGTTTTATGAAGGCCTTGATGAAGCTGATAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTc                                                                                                                    <  2:961529/140‑1 (MQ=255)                    gAACTGTTTTATGAAGACCTTGATGAAGCTGATAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAAtt                                                                                                                     <  1:888166/137‑1 (MQ=255)                    gAACTGTTTTATGAAGACCTTGATGAAGCTGATAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAAtt                                                                                                                     >  2:888166/1‑137 (MQ=255)                    gAACTGTTTTATGAAGACCTTGATGAAGCTGATAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCaa                                                                                                                  <  2:456413/140‑1 (MQ=255)                               tGAAGACCTTGATGAAGCTGATAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGa                                                                                                         <  2:631182/138‑1 (MQ=255)                               tGAAGACCTTGATGAAGCTGATAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGa                                                                                                         >  1:631182/1‑138 (MQ=255)                                       ttGATGAAGCTGATAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGaa                                                                                               <  1:861579/140‑1 (MQ=255)                                         gatgaAGCTGATAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAAt                                                                                             >  1:162707/1‑140 (MQ=255)                                         gatgaAGCTGATAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAAt                                                                                             >  2:1261255/1‑140 (MQ=255)                                         gatgaAGCTGATAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAAt                                                                                             >  2:1024090/1‑140 (MQ=255)                                              aGCTGATAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTcgcg                                                                                        >  1:813783/1‑140 (MQ=255)                                                 tGATAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATc                                                                                     <  1:295847/140‑1 (MQ=255)                                                 tGATAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATc                                                                                     <  1:418595/140‑1 (MQ=255)                                                   aTAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATcgc                                                                                   <  2:162707/140‑1 (MQ=255)                                                   aTAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATcgc                                                                                   >  2:1046681/1‑140 (MQ=255)                                                      aCATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCggg                                                                                <  1:482662/140‑1 (MQ=255)                                                          tGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGa                                                                            >  2:39231/1‑140 (MQ=255)                                                           gAGGCAATCCGCAAATTGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGAt                                                                           >  1:525477/1‑140 (MQ=255)                                                             ggTAATCCGCAAATTGCTGGATACACTCAATGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGAttt                                                                         >  2:637598/1‑140 (MQ=255)                                                                aaTCCGCAAATTGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑g                                                                    <  1:1024090/140‑1 (MQ=255)                                                                 aTCCGCAAATTGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑gc                                                                   >  2:942846/1‑140 (MQ=255)                                                                  tCCGCAAATTGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATc                                                                                     <  2:62432/123‑1 (MQ=255)                                                                  tCCGCAAATTGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATc                                                                                     >  1:62432/1‑123 (MQ=255)                                                                  tCCGCAAATTGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑gcg                                                                  >  1:879919/1‑140 (MQ=255)                                                                  tCCGCAAATTGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑gcg                                                                  >  2:975273/1‑140 (MQ=255)                                                                  tCCGCAAATTGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑gcg                                                                  <  1:39231/140‑1 (MQ=255)                                                                  tCCGCAAATTGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑gcg                                                                  >  1:166309/1‑140 (MQ=255)                                                                     gCAAATTGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑GCGCaa                                                               <  1:539162/140‑1 (MQ=255)                                                                       aaaTTGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑GCGCAACt                                                             <  1:1269024/140‑1 (MQ=255)                                                                          ttGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑GCGCAACTGGa                                                          <  1:407287/140‑1 (MQ=255)                                                                           tGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑GCGCAACTGGAt                                                         >  1:1109052/1‑140 (MQ=255)                                                                                    cacTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑GCGCAACTGGATTGCGAacac                                                <  1:162715/140‑1 (MQ=255)                                                                                     acTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑GCGCAACTGGATTGCGAACAcg                                               >  2:528667/1‑140 (MQ=255)                                                                                     acTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑GCGCAACTGGATTGCGAACAcg                                               >  2:33996/1‑140 (MQ=255)                                                                                     acTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑GCGCAACTGGATTGCGAACAcg                                               >  1:948505/1‑140 (MQ=255)                                                                                          aaGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑GCGCAACTGGATTGCGAACAcgttgcg                                          >  2:298718/1‑140 (MQ=255)                                                                                           aGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑GCGCAACTGGATTGCGAACAcgttgcgt                                         >  1:670989/1‑140 (MQ=255)                                                                                           aGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑GCGCAACTGGATTGCGAACAcgttgcgt                                         >  1:387262/1‑140 (MQ=255)                                                                                           aGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑GCGCAACTGGATTGCGAACAcgttgcgt                                         >  2:688638/1‑140 (MQ=255)                                                                                           aGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑GCGCAACTGGATTGCGAACAcgttgcgt                                         >  2:653166/1‑140 (MQ=255)                                                                                            gCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑GCGCAACTGGATTGCGAACAcgttgcgtt                                        <  1:261276/140‑1 (MQ=255)                                                                                              cTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑GCGCAACTGGATTGCGAACACGTTGCGTTGa                                      <  2:817073/140‑1 (MQ=255)                                                                                              cTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑GCGCAACTGGATTGCGAACACGTTGCGTTGa                                      <  1:688638/140‑1 (MQ=255)                                                                                               tCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑GCGCAACTGGATTGCGAACACGTTGCGTTGaa                                     <  2:894863/140‑1 (MQ=255)                                                                                               tCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑GCGCAACTGGATTGCGAACACGTTGCGTTGaa                                     <  2:525477/140‑1 (MQ=255)                                                                                               tCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑GCGCAACTGGATTGCGAACACGTTGCGTTGaa                                     >  1:220550/1‑140 (MQ=255)                                                                                                cGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑GCGCAACTGGATTGCGAACACGTTGCGTTGAAc                                    >  1:110234/1‑140 (MQ=255)                                                                                                 gttaaTGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑GCGCAACTGGATTGCGAACACGTTGCGTTGAACg                                   >  1:945015/5‑140 (MQ=255)                                                                                                 gTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑GCGCAACTGGATTGCGAACAcgttgcg                                          <  1:306511/133‑1 (MQ=255)                                                                                                 gTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑GCGCAACTGGATTGCGAACAcgttgcg                                          >  2:306511/1‑133 (MQ=255)                                                                                                   tGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑GCGCAACTGGATTGCGAACACGTTGCGTTGAACGAt                                 <  2:166309/140‑1 (MQ=255)                                                                                                       ggAAGAAAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑GCGCAACTGGATTGCGAACACGTTGCGTTGAACGATTTaa                             >  1:1008524/1‑140 (MQ=255)                                                                                                          agaaAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑GCGCAACTGGATTGCGAACACGTTGCGTTGAACGATTTAAGtt                          >  1:977522/1‑140 (MQ=255)                                                                                                           gaaAATCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑GCGCAACTGGATTGCGAACACGTTGCGTTGAACGATTTAAGTTa                         >  1:788655/1‑140 (MQ=255)                                                                                                                tCAGATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑GCGCAACTGGATTGCGAACACGTTGCGTTGAACGATTTAAGTTATTTCg                    <  1:298718/140‑1 (MQ=255)                                                                                                                   gATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑GCGCAACTGGATTGCGAACACGTTGCGTTGAACGAtt                                <  2:941440/125‑1 (MQ=255)                                                                                                                   gATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑GCGCAACTGGATTGCGAACACGTTGCGTTGAACGAtt                                >  1:941440/1‑125 (MQ=255)                                                                                                                   gATGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑GCGCAACTGGATTGCGAACACGTTGCGTTGAACGATTTAAGTTATTTCGTTg                 >  1:1039795/1‑140 (MQ=255)                                                                                                                     tGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑GCGCAACTGGATTGCGAACACGTTGCGTTGAACGATTTAAGTTATTTCg                    >  2:1155111/1‑135 (MQ=255)                                                                                                                     tGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑GCGCAACTGGATTGCGAACACGTTGCGTTGAACGATTTAAGTTATTTCg                    <  1:1155111/135‑1 (MQ=255)                                                                                                                     tGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑GCGCAACTGGATTGCGAACACGTTGCGTTGAACGATTTAAGTTATTTCGTTGaa               <  2:387262/140‑1 (MQ=255)                                                                                                                     tGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑GCGCAACTGGATTGCGAACACGTTGCGTTGAACGATTTAAGTTATTTCGTTGaa               >  1:406166/1‑140 (MQ=255)                                                                                                                     tGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑GCGCAACTGGATTGCGAACACGTTGCGTTGAACGATTTAAGTTATTTCGTTGaa               <  2:879919/140‑1 (MQ=255)                                                                                                                     tGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑GCGCAACTGGATTGCGAACACGTTGCGTTGAACGATTTAAGTTATTTCGTTGaa               <  2:110234/140‑1 (MQ=255)                                                                                                                     tGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑GCGCAACTGGATTGCGAACACGTTGCGTTGAACGATTTAAGTTATTTCGTTGaa               <  1:33996/140‑1 (MQ=255)                                                                                                                     tGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑GCGCAACTGGATTGCGAACACGTTGCGTTGAACGATTTAAGTTATTTCGTTGaa               <  2:597198/140‑1 (MQ=255)                                                                                                                     tGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑GCGCAACTGGATTGCGAACACGTTGCGTTGAACGATTTAAGTTATTTCGTTGa                >  2:1035086/1‑139 (MQ=255)                                                                                                                     tGGAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑GCGCAACTGGATTGCGAACACGTTGCGTTGAACGATTTAAGTTATTTCGTTGa                <  1:1035086/139‑1 (MQ=255)                                                                                                                      ggAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑GCGCAACTGGATTGCGAACACGTTGCGTTGAACGATTTAAGTTATTTCGTTGaa               >  1:340107/1‑139 (MQ=255)                                                                                                                      ggAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑GCGCAACTGGATTGCGAACACGTTGCGTTGAACGATTTAAGTTATTTCGTTGaa               <  2:340107/139‑1 (MQ=255)                                                                                                                       gAGCTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTATGCGCGCAACTGGATTGCGAACACGTTGCGTTGAACGATTTAAGTTATTTCGTTGaa               <  2:813783/140‑1 (MQ=255)                                                                                                                          cTGGCGTTACGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑GCGCAACTGGATTGCGAACACGTTGCGTTGAACGATTTAAGTTATTTCGTTGAACAgtg          <  2:626391/140‑1 (MQ=255)                                                                                                                                  aCGCTCCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑GCGCAACTGGATTGCGAACACGTTGCGTTGAACGATTTAAGTTATTTCGTTGAACAGTGTCCGGAAg  >  2:1102175/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                          AAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGAAGCTGATAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGGAGCTGGCGTTACGCACCAGCGAAGCTTTATTACAATTCAACCCTGAAGATCCCTATGAAATTCGCGATCGCGGGTTGATTTAT‑‑GCGCAACTGGATTGCGAACACGTTGCGTTGAACGATTTAAGTTATTTCGTTGAACAGTGTCCGGAAG  >  NC_000913/1267793‑1268061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑