Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,874,250	G→A	intergenic (‑68/‑102)	yoaI ← / → yeaL	protein YoaI/conserved inner membrane protein YeaL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,874,250	0	G	A	100.0%	229.9 / NA	65	intergenic (‑68/‑102)	yoaI/yeaL	protein YoaI/conserved inner membrane protein YeaL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (37/28);  total (37/28)

CCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGTCACTTTGCTGATCCTGCTCGGG  >  NC_000913/1874113‑1874384                                                                                                                                          |                                                                                                                                       ccAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAAtt                                                                                                                                      <  2:275776/140‑1 (MQ=255)    gTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAATTCGc                                                                                                                                   <  2:762870/140‑1 (MQ=255)     tACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAATTCGcc                                                                                                                                  >  1:1086117/1‑140 (MQ=255)     tACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAATTCGcc                                                                                                                                  >  2:589820/1‑140 (MQ=255)     tACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAATTCGcc                                                                                                                                  >  1:233442/1‑140 (MQ=255)             cAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAATTCGCCTGAttttt                                                                                                                          >  2:841301/1‑140 (MQ=255)               aTGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAATTCGCCTGATTTTTTa                                                                                                                        >  1:25070/1‑140 (MQ=255)               aTGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAATTCGCCTGATTTTTTa                                                                                                                        >  2:576275/1‑140 (MQ=255)               aTGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAATACGCCTGATTTTTTa                                                                                                                        >  1:1122441/1‑140 (MQ=255)                  gCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGc                                                                                                                     >  1:659250/1‑140 (MQ=255)                     aaaaaCGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGCaaa                                                                                                                  <  1:801889/140‑1 (MQ=255)                                       aTCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTa                                                                                                <  2:1086117/140‑1 (MQ=255)                                            tgtCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTActtcc                                                                                           <  1:321335/140‑1 (MQ=255)                                            tgtCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTActtcc                                                                                           >  2:321335/1‑140 (MQ=255)                                              tctcGACAAACATTTGATCGTTCATATCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTActtcctt                                                                                         >  1:200422/1‑140 (MQ=255)                                              tctcGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTActtcctt                                                                                         >  1:421422/1‑140 (MQ=255)                                                 cGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTtccttcct                                                                                      >  1:13663/1‑140 (MQ=255)                                                 cGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTtccttcct                                                                                      >  1:1163791/1‑140 (MQ=255)                                                     aaaCATTTGATCGTTCATATCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTgcag                                                                                  <  2:200422/140‑1 (MQ=255)                                                     aaaCATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTgcag                                                                                  <  2:730040/140‑1 (MQ=255)                                                      aaCATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTtccttcct                                                                                      <  2:1018922/135‑1 (MQ=255)                                                      aaCATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTtccttcct                                                                                      >  1:1018922/1‑135 (MQ=255)                                                      aaCATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTgcagc                                                                                 <  2:25070/140‑1 (MQ=255)                                                           ttGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCg                                                                            <  1:295905/140‑1 (MQ=255)                                                           ttGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCg                                                                            <  2:13663/140‑1 (MQ=255)                                                           ttGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCg                                                                            <  2:1163791/140‑1 (MQ=255)                                                              gtccttCATAGCATGCCCCCGGGAGATAGCTGTTTTCGCGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAGCGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTaa                                                                         <  1:886450/136‑1 (MQ=255)                                                                   tCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTaa                                                                    >  2:583086/1‑140 (MQ=255)                                                                    cATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAg                                                                   <  1:448201/140‑1 (MQ=255)                                                                     aTAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGc                                                                  <  1:576275/140‑1 (MQ=255)                                                                       aGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAg                                                                >  2:616921/1‑140 (MQ=255)                                                                            gCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCtta                                                           >  2:957823/1‑140 (MQ=255)                                                                            gCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCtta                                                           >  1:1119510/1‑140 (MQ=255)                                                                             cccccGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCttat                                                          >  2:978115/1‑140 (MQ=255)                                                                             cccccGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCttat                                                          >  1:903478/1‑140 (MQ=255)                                                                             cccccGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCttat                                                          >  1:683790/1‑140 (MQ=255)                                                                                       aTAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTgg                                                >  1:193892/1‑140 (MQ=255)                                                                                       aTAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTgg                                                >  2:1158001/1‑140 (MQ=255)                                                                                         aGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCa                                              >  2:349735/1‑140 (MQ=255)                                                                                             gTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGa                                          >  1:913159/1‑140 (MQ=255)                                                                                             gTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGa                                          <  2:233442/140‑1 (MQ=255)                                                                                             gTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGa                                          <  2:193892/140‑1 (MQ=255)                                                                                              ttttCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGaa                                         <  1:841301/140‑1 (MQ=255)                                                                                                ttCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAAc                                       >  2:1013379/1‑140 (MQ=255)                                                                                                   aCGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCAc                                    <  2:659250/140‑1 (MQ=255)                                                                                                          tGTTAGCGTGCACAAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGttt                             <  2:1119510/140‑1 (MQ=255)                                                                                                               gCGTGCACAAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGt                        >  2:486437/1‑140 (MQ=255)                                                                                                               gCGTGCACAAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGt                        <  2:776090/140‑1 (MQ=255)                                                                                                                 gTGCACAAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGTCa                      <  2:913159/140‑1 (MQ=255)                                                                                                                 gTGCACAAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGTCa                      <  1:349735/140‑1 (MQ=255)                                                                                                                  tGCACAAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGTCAc                     <  1:22056/140‑1 (MQ=255)                                                                                                                  tGCACAAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGTCAc                     <  1:616921/140‑1 (MQ=255)                                                                                                                   gCACAAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGTCa                      <  2:295878/138‑1 (MQ=255)                                                                                                                   gCACAAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGTCa                      >  1:295878/1‑138 (MQ=255)                                                                                                                   gCACAAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGTCACt                    >  1:451513/1‑140 (MQ=255)                                                                                                                    cacaAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGTCACtt                   >  2:683073/1‑140 (MQ=255)                                                                                                                    cacaAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGTCACtt                   >  1:575955/1‑140 (MQ=255)                                                                                                                     acaAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGTCACttt                  >  1:443721/1‑140 (MQ=255)                                                                                                                     acaAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGTCACttt                  >  1:703789/1‑140 (MQ=255)                                                                                                                      caAATGGCAGTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGTCACTTTg                 <  1:486437/140‑1 (MQ=255)                                                                                                                            gCAGTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGTCACTTTGCTGATc           >  1:229902/1‑140 (MQ=255)                                                                                                                             cAGCTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGTCACTTTGCTGATcc          >  2:852093/1‑140 (MQ=255)                                                                                                                               gTTTGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGTCACTTTGCTGATCCTg        <  2:229902/140‑1 (MQ=255)                                                                                                                                 ttGATGACAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGTCACTTTGCTGATCCTGCt      <  2:703789/140‑1 (MQ=255)                                                                                                                                    atgaCAATTCGCCTGATTTTTTATGCAAAAAACGTAATGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTCGCAAGGAAACCACGATGTTTGATGTCACTTTGCTGATCCTGCTCgg   >  2:522773/1‑140 (MQ=255)                                                                                                                                     tgaCAATTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGTCACTTTGCTGATCCTGCTCggg  >  1:880171/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       CCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGTCACTTTGCTGATCCTGCTCGGG  >  NC_000913/1874113‑1874384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑