Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,925,209	G→T	P34P (CCG→CCT)	holE →	DNA polymerase III subunit theta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,925,209	0	G	T	100.0%	201.7 / NA	57	P34P (CCG→CCT)	holE	DNA polymerase III subunit theta
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (27/30);  total (27/30)

AGTAACTTTTGCCCGCGAATGATGAGGAGATTAAGAATGCTGAAGAATCTGGCTAAACTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGGTCAATCTGTCACGTTTACCTTACGAGCCCAAACTTAAATAAAAC  >  NC_000913/1925072‑1925341                                                                                                                                          |                                                                                                                                     aGTAACTTTTGCCCGCGAATGATGAGGAGATTAAGAATGCTGAAGAATCTGGCTAAACTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCTGt                                                                                                                                    >  2:622433/1‑140 (MQ=255) aGTAACTTTTGCCCGCGAATGATGAGGAGATTAAGAATGCTGAAGAATCTGGCTAAACTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCTGt                                                                                                                                    >  2:214130/1‑140 (MQ=255) aGTAACTTTTGCCCGCGAATGATGAGGAGATTAAGAATGCTGAAGAATCTGGCTAAACTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCTGt                                                                                                                                    >  1:785217/1‑140 (MQ=255) aGTAACTTTTGCCCGCGAATGATGAGGAGATTAAGAATGCTGAAGAATCTGGCTAAACTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCTGt                                                                                                                                    >  1:420077/1‑140 (MQ=255) aGTAACTTTTGCCCGCGAATGATGAGGAGATTAAGAATGCTGAAGAATCTGGCTAAACTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCTGt                                                                                                                                    >  1:405938/1‑140 (MQ=255)   tAACTTTTGCCCGCGAATGATGAGGAGATTAAGAATGCTGAAGAATCTGGCTAAACTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCTGTGa                                                                                                                                  >  1:255014/1‑140 (MQ=255)       ttttGCCCGCGAATGATGAGGAGATTAAGAATGCTGAAGAATCTGGCTAAACTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCTGTGATCGc                                                                                                                              >  2:1002230/1‑140 (MQ=255)         ttGCCCGCGACTGATGAGGAGATTAAGAATGCTGAAGGATCTGGCTAAACTGGATCAAACAGAAATGGAAAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCTGTGATCGCTg                                                                                                                            >  2:669885/1‑140 (MQ=255)         ttGCCCGCGAATGATGAGGAGATTAAGAATGCTGAAGAATCTGGCTAAACTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCTGTGATCGCTg                                                                                                                            <  2:732253/140‑1 (MQ=255)           gCCCGCGAATGATGAGGAGATTAAGAATGCTGAAGAATCTGGCTAAACTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCTGTGATCGCTGaa                                                                                                                          <  1:142146/140‑1 (MQ=255)                cgAATGATGAGGAGATTAAGAATGCTGAAGAATCTGGCTAAACTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCTGTGATCGCTGAAGCGGt                                                                                                                     <  2:1064027/140‑1 (MQ=255)                cgAATGATGAGGAGATTAAGAATGCTGAAGAATCTGGCTAAACTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCTGTGATCGCTGAAGCGGt                                                                                                                     <  1:559879/140‑1 (MQ=255)                cgAATGATGAGGAGATTAAGAATGCTGAAGAATCTGGCTAAACTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCTGTGATAGCTGAAGCGGt                                                                                                                     <  1:265985/140‑1 (MQ=255)                    tgatgaGGAGATTAAGAATGCTGAAGAATCTGGCTAAACTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCTGTGATCGCTGAAGCGGTTGaa                                                                                                                 <  2:405938/140‑1 (MQ=255)                           gagaTTAAGAATGCTGAAGAATCTGGCTAAACTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCTGTGATCGCTGAAGCGGTTGAACGTGAAc                                                                                                          <  1:214130/140‑1 (MQ=255)                                tAAGAATGCTGAAGAATCTGGCTAAACTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCTGTGATCGCTGAAGCGGTTGAACGTGAACAGCCt                                                                                                     >  2:700322/1‑140 (MQ=255)                                   gAATGCTGAAGAATCTGGCTAAACTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCTGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGaa                                                                                                  >  2:344784/1‑140 (MQ=255)                                      tGCTGAAGAATCTGGCTAAACTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCTGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACAt                                                                                               >  1:64562/1‑140 (MQ=255)                                        cTGAAGAATCTGGCTAAACTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCTGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACAttt                                                                                             >  1:345444/1‑140 (MQ=255)                                        cTGAAGAATCTGGCTAAACTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCTGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACAttt                                                                                             >  1:1019223/1‑140 (MQ=255)                                               aTCTGGCTAAACTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCTGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGc                                                                                      <  1:622433/140‑1 (MQ=255)                                                 cTGGCTAAACTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCTGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTg                                                                                    >  1:167519/1‑140 (MQ=255)                                                     cTAAACTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCTGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGttt                                                                                >  1:880452/1‑140 (MQ=255)                                                             gATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGACCGCTACAATATGCCTGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCg                                                                        <  2:477447/140‑1 (MQ=255)                                                              aTCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCTGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCgg                                                                       >  1:964653/1‑140 (MQ=255)                                                                 aaaCAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCTGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCtt                                                                    <  1:903337/140‑1 (MQ=255)                                                                  aaCAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCTGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTa                                                                   <  1:397168/140‑1 (MQ=255)                                                                  aaCAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCTGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTa                                                                   >  2:11978/1‑140 (MQ=255)                                                                       aaaTGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCTGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGcc                                                              >  2:600548/1‑140 (MQ=255)                                                                              tAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCTGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGtt                                                       >  2:480507/1‑140 (MQ=255)                                                                                      aTGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCTGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGtt                                                       >  2:451521/1‑132 (MQ=255)                                                                                      aTGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCTGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGtt                                                       <  1:451521/132‑1 (MQ=255)                                                                                      aTGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCTGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCg                                               >  2:200222/1‑140 (MQ=255)                                                                                       tGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCTGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCgg                                              <  1:480507/140‑1 (MQ=255)                                                                                           gATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCTGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGGTCaa                                          <  1:700322/140‑1 (MQ=255)                                                                                            aTTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCTGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGGTCAAt                                         >  2:675989/1‑140 (MQ=255)                                                                                             tttGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCTGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGGTCAATc                                        >  2:831502/1‑140 (MQ=255)                                                                                              ttGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCTGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGGTCAATCt                                       <  1:831502/140‑1 (MQ=255)                                                                                               tGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCTGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGGTCAATCTg                                      <  2:1024739/140‑1 (MQ=255)                                                                                               tGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCTGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGGTCAATCTg                                      <  1:200222/140‑1 (MQ=255)                                                                                                ggcggcggcCGGGGTGGCATTTAAAGAACGCTACAATATGCCTGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGGTCAATCTGt                                     <  2:697566/140‑1 (MQ=255)                                                                                                    gcggcCGGGGTGGCATTTAAAGAACGCTACAATATGCCTGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGGTCAATCTGTCACg                                 <  1:675989/140‑1 (MQ=255)                                                                                                      ggcCGGGGTGGCATTTAAAGAACGCTACAATATGCCTGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGGTCAATCTGTCACGtt                               <  2:1019223/140‑1 (MQ=255)                                                                                                             gTGGCATTTAAAGAACGCTACAATATGCCTGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGGTCAATCTGTCACGTTTACCTTa                        <  1:669885/140‑1 (MQ=255)                                                                                                              tGGCATTTAAAGAACGCTACAATATGCCTGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGGTCAATCTGTCACGTTTACCTTAc                       <  2:205342/140‑1 (MQ=255)                                                                                                              tGGCATTTAAAGAACGCTACAATATGCCTGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGGTCAATCTGTCACGTTTACCTTAc                       <  1:101045/140‑1 (MQ=255)                                                                                                              tGGCATTTAAAGAACGCTACAATATGCCTGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGGTCAATCTGTCACGTTTACCTTAc                       <  2:64562/140‑1 (MQ=255)                                                                                                                        aGAACGCTACAATATGCCTGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGGTCAATCTGTCACGTTTACCTTACGAGCCCAAAc             <  1:1002230/140‑1 (MQ=255)                                                                                                                           aCGCTACAATATGCCTGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGGTCAATCTGTCACGTTTACCTTACGAGCCCAAACTta          >  2:548624/1‑140 (MQ=255)                                                                                                                            cGCTACAATATGCCTGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGGTCAATCTGTCACGTTTACCTTACGAGCCCAAACTtaa         >  1:479042/1‑140 (MQ=255)                                                                                                                              cTACAATATGCCTGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGGTCAATCTGTCACGTTTACCTTACGAGCCCAAACTtaaat       <  1:600548/140‑1 (MQ=255)                                                                                                                              cTACAATATGCCTGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGGTCAATCTGTCACGTTTACCTTACGAGCCCAAACTtaaat       >  2:537771/1‑140 (MQ=255)                                                                                                                              cTACAATATGCCTGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGGTCAATCTGTCACGTTTACCTTACGAGCCCAAACTtaaat       <  2:479042/140‑1 (MQ=255)                                                                                                                              cTACAATATGCCTGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGGTCAATCTGTCACGTTTACCTTACGAGCCCAAACTtaaat       <  2:167519/140‑1 (MQ=255)                                                                                                                                aCAATATGCCTGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGGTCAATCTGTCACGTTTACCTTACGAGCCCAAACTtaaataa     <  2:760516/140‑1 (MQ=255)                                                                                                                                 cAATATGCCTGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGGTCAATCTGTCACGTTTACCTTACGAGCCCAAACTtaaataaa    >  1:458552/1‑140 (MQ=255)                                                                                                                                   atacGCCTGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGATCAATCTGTCACGTTTACCTTACGAGCCCAAACTTAAATAAAAc  <  2:255014/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                     AGTAACTTTTGCCCGCGAATGATGAGGAGATTAAGAATGCTGAAGAATCTGGCTAAACTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGGTCAATCTGTCACGTTTACCTTACGAGCCCAAACTTAAATAAAAC  >  NC_000913/1925072‑1925341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑