Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,165,070	T→C	intergenic (+72/‑119)	baeR → / → yegP	DNA‑binding transcriptional activator BaeR/DUF1508 domain‑containing protein YegP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,165,070	0	T	C	100.0%	180.9 / NA	48	intergenic (+72/‑119)	baeR/yegP	DNA‑binding transcriptional activator BaeR/DUF1508 domain‑containing protein YegP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/24);  total (24/24)

CCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAGTTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAA  >  NC_000913/2164941‑2165206                                                                                                                                  |                                                                                                                                         ccGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAGTTTTAGCGACATTATTTTGTTAGCCGGAGAAGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGCCAGCAAACTg                                                                                                                                >  2:1139535/1‑140 (MQ=255)   gcgcCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAGTTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGCCAGCAAACTGAc                                                                                                                              >  2:322838/1‑140 (MQ=255)   gcgcCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAGTTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGCCAGCAAACTGAc                                                                                                                              >  1:95993/1‑140 (MQ=255)   gcgcCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAGTTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGCCAGCAAACTGAc                                                                                                                              >  2:243559/1‑140 (MQ=255)   gcgcCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAGTTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGCCAGCAAACTGAc                                                                                                                              >  2:448535/1‑140 (MQ=255)     gcCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAGTTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGCCAGCAAACTGACAt                                                                                                                            >  2:1125024/1‑140 (MQ=255)                  tCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAGTTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGCCAGCAAACTGACATTATCTCTCCGccc                                                                                                               >  2:289664/1‑140 (MQ=255)                   cGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAGTTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGCCAGCAAACTGACATTATCTCTCCGCCCt                                                                                                              >  1:568066/1‑140 (MQ=255)                               ggAAGCCGACGCCTGCCGCATCGTTTAGTTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGCCAGCAAACTGACATTATCTCTCCGCCCTggca                                                                                                          >  2:775371/1‑132 (MQ=255)                               ggAAGCCGACGCCTGCCGCATCGTTTAGTTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGCCAGCAAACTGACATTATCTCTCCGCCCTggca                                                                                                          <  1:775371/132‑1 (MQ=255)                                    ccGACGCCTGCCGCATCGTTTAGTTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGCCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAAt                                                                                             >  2:740122/1‑140 (MQ=255)                                    ccGACGCCTGCCGCATCGTTTAGTTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGCCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAAt                                                                                             >  2:113473/1‑140 (MQ=255)                                              ccGCATCGTTTAGTTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGCCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACt                                                                                   <  1:937109/140‑1 (MQ=255)                                                   tCGTTTAGTTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGCCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAAt                                                                              <  2:165615/140‑1 (MQ=255)                                                             ttAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGCCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAg                                                                    <  2:130896/140‑1 (MQ=255)                                                             ttAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGCCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAg                                                                    <  1:1125024/140‑1 (MQ=255)                                                              tAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGCCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAg                                                                    <  2:139704/139‑1 (MQ=255)                                                              tAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGCCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAg                                                                    >  1:139704/1‑139 (MQ=255)                                                               aGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGCCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCa                                                                  <  1:113473/140‑1 (MQ=255)                                                               aGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGCCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCa                                                                  <  1:740122/140‑1 (MQ=255)                                                                gCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGCCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCAc                                                                 >  2:243463/1‑140 (MQ=255)                                                                gCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGCCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCAc                                                                 >  1:252198/1‑140 (MQ=255)                                                                         ttttGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGCCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATg                                                        <  2:872164/140‑1 (MQ=255)                                                                              ttAGCCGGAGATGGCGGTCAGACCAGATCCGGCAACATTATCCCACGCATGGCCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGtt                                                   >  1:338191/1‑140 (MQ=255)                                                                                 gCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGCCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTc                                                >  2:181203/1‑140 (MQ=255)                                                                                 gCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGCCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTc                                                >  2:1080393/1‑140 (MQ=255)                                                                                  ccGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGCCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCg                                               <  1:322838/140‑1 (MQ=255)                                                                                       gaTGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGCCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATaca                                          <  2:276238/140‑1 (MQ=255)                                                                                       gaTGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGCCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATaca                                          <  2:184654/140‑1 (MQ=255)                                                                                           gCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGCCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAAt                                      <  2:338191/140‑1 (MQ=255)                                                                                             gTTCAGACCAGATCCGGCAACATTATCCCACGCATGGCCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAAttt                                    <  1:541718/140‑1 (MQ=255)                                                                                                cAGACCAGATCCGGCAACATTATCCCACGCATGGCCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTtcaa                                 >  1:1014372/1‑140 (MQ=255)                                                                                                 ataCCAGATCCGGCAACATTATCCCACGCATGGCCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTtcaat                                <  1:371421/138‑1 (MQ=255)                                                                                                  gACCAGATCCGGCAACATTATCCCACGCATGGCCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTtcaatc                               <  1:1082466/140‑1 (MQ=255)                                                                                                       gATCCGGCAACATTATCCCACGCATGGCCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGa                          <  1:32756/140‑1 (MQ=255)                                                                                                       gATCCGGCAACATTATCCCACGCATGGCCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGa                          <  1:243463/140‑1 (MQ=255)                                                                                                        aTCCGGCAACATTATCCCACGCATGGCCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAg                         >  1:54281/1‑140 (MQ=255)                                                                                                        aTCCGGCAACATTATCCCACGCATGGCCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAACCGCTCATACTTTAATCGTTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGa                          >  2:373580/1‑139 (MQ=255)                                                                                                        aTCCGGCAACATTATCCCACGCATGGCCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAACCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGa                          <  1:373580/139‑1 (MQ=255)                                                                                                             gCAACATTATCCCACGCATGGCCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCAtt                    >  1:737788/1‑140 (MQ=255)                                                                                                             gCAACATTATCCCACGCATGGCCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCAtt                    >  1:335901/1‑140 (MQ=255)                                                                                                             gCAACATTATCCCACGCATGGCCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCAtt                    >  2:769632/1‑140 (MQ=255)                                                                                                                  aTTATCCCACGCATGGCTAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGc               >  1:679578/1‑140 (MQ=255)                                                                                                                     aTCCCACGCATGGCCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCtgg            <  1:448535/140‑1 (MQ=255)                                                                                                                     aTCCCACGCATGGCCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCtgg            <  1:243559/140‑1 (MQ=255)                                                                                                                               tGGCCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGaa  <  2:737788/140‑1 (MQ=255)                                                                                                                               tGGCCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGaa  <  1:1139535/140‑1 (MQ=255)                                                                                                                               tGGCCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGaa  <  2:95993/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                         CCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAGTTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAA  >  NC_000913/2164941‑2165206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑