Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4350CT31.1% 70.6 / 27.6 45intergenic (‑179/‑28)ttdR/ttdADNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (0/31);  new base T (14/0);  total (14/31)
Fisher's exact test for biased strand distribution p-value = 5.99e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAA  >  NC_000913/3206305‑3206574
                                                                                                                                  |                                                                                                                                           
tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTcaaacaaaa                                                                                                                                    >  2:613071/1‑139 (MQ=255)
        ccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAAc                                                                                                                            <  1:605363/140‑1 (MQ=255)
          tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTg                                                                                                                          <  2:1007745/140‑1 (MQ=255)
            ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTATACAAAAACAAAAAATggg                                                                                                                        >  2:1287692/1‑139 (MQ=255)
            ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGa                                                                                                                        <  1:1077294/140‑1 (MQ=255)
            ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTTCGAAAAAAAAAAAAAATGGa                                                                                                                        >  2:706363/1‑140 (MQ=255)
             tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                                       <  1:679788/140‑1 (MQ=255)
             tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                                       <  1:774104/140‑1 (MQ=255)
             tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                                       <  2:144335/140‑1 (MQ=255)
                    cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGAGGTGGCat                                                                                                                >  2:90205/1‑140 (MQ=255)
                      atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCCAACAAAAACAAAAAAAGGAGTTGCCaagg                                                                                                              >  1:261848/1‑137 (MQ=255)
                      atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGGAAAATACAAAAAAAGGAGTTGGCatga                                                                                                              >  1:148338/1‑140 (MQ=255)
                      atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCatga                                                                                                              <  1:1287692/140‑1 (MQ=255)
                        attcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCatgatg                                                                                                            <  2:755711/140‑1 (MQ=255)
                          tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAg                                                                                                          <  1:103006/140‑1 (MQ=255)
                          tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAg                                                                                                          <  1:273812/140‑1 (MQ=255)
                             ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                       <  2:1056285/140‑1 (MQ=255)
                              tcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGaa                                                                                                      <  1:163603/140‑1 (MQ=255)
                                 tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCCGACAAAAAAAAAAAAAGGAGGTGGCCTGATGAGCGgaaaa                                                                                                   >  1:1514615/1‑138 (MQ=255)
                                 tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAATTTTTTCAAACAAATACAAAAAATTGAGGTGCCAAGATTAGCGAAAga                                                                                                   >  1:1475475/1‑139 (MQ=255)
                                   ttttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAACAAAAAATGGAGTTGCCATGATGAGCgaaagaaa                                                                                                 >  1:1321309/1‑140 (MQ=255)
                                            tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACa                                                                                        <  1:613071/140‑1 (MQ=255)
                                              aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                                      <  1:90205/140‑1 (MQ=255)
                                              aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                                      <  1:1139465/140‑1 (MQ=255)
                                                tCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCa                                                                                    <  1:619414/140‑1 (MQ=255)
                                                   cGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTg                                                                                 <  2:817035/140‑1 (MQ=255)
                                                     acaggTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGaa                                                                               <  1:423372/136‑1 (MQ=255)
                                                     aaCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGaa                                                                               <  1:777134/140‑1 (MQ=255)
                                                                  atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTAGACAAAAAAAAAAAAAGGAGGTGGCCTGATGAGGGAAAGAAAAAAAAAAAAAGCAATGAATAAGATGACagag                                                                  >  2:1194018/1‑140 (MQ=255)
                                                                  atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAAAAAAAACTGGAGGTGCCATGATGGGGGGAAAGAATAAACAAAAAGCAGGGAAAAAATTGAAagaa                                                                  >  2:100431/1‑139 (MQ=255)
                                                                   tatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAgaga                                                                 <  1:1194018/140‑1 (MQ=255)
                                                                    atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGAt                                                                <  2:839181/140‑1 (MQ=255)
                                                                    atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGAt                                                                <  1:1397496/140‑1 (MQ=255)
                                                                    atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGAt                                                                <  1:936529/140‑1 (MQ=255)
                                                                    atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTTAGACAAAAAAAAAAAATGGAGGTGCCATGATGGGGGAAAAGAATAAACAACAAGCAAAGAAAAAAATGACagaggt                                                                >  2:936529/1‑138 (MQ=255)
                                                                    atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCCGACAAAAAAAAAAAAAGGAGGTGGCATGATTAGCGAAAAAAAAAAAAAAAAAGCAATGAAAAAAATTACAgagaa                                                                >  2:500815/1‑139 (MQ=255)
                                                                      tCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTg                                                              <  1:611444/140‑1 (MQ=255)
                                                                           gTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAAAAAAAAAAGGGGTTGGCATGATGAGGGAAAGGAAAAAAAAAAAAGCAGGGAAAAAAATGACAGAGAATGTCGCt                                                         >  2:1154770/1‑140 (MQ=255)
                                                                           gTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAATTTTTTCAAACAAAAAAAAAAAATGGAGTTGGCATGATGAGGGGAAGAAATAAAAAAAAAGCAGGGAAAAAATTGACAGAGATTGTTGCt                                                         >  2:1110806/1‑140 (MQ=255)
                                                                            tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATTGAGTTGGCATGATGAGCGGAAGTAATAAACAAAAAGCAGGGAATAAATTGACAGAGATTGTCGCTa                                                        >  2:1408389/1‑140 (MQ=255)
                                                                                   aaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAAAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAc                                                 <  2:1167277/140‑1 (MQ=255)
                                                                                    aaTATAAAACCACATTTTTAGGGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAAAGAGATTGTCGCTAACTTTAcc                                                <  2:1475475/140‑1 (MQ=255)
                                                                                                       aGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGaa                             <  1:500815/140‑1 (MQ=255)
                                                                                                       aGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTGGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGaa                             <  2:1273028/140‑1 (MQ=255)
                                                                                                        gTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAATTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAAt                            <  2:142816/140‑1 (MQ=255)
                                                                                                           gTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAACAAAAAATGGGGGTGGCATGGTGAGGGGAAAAAAAAAACAAAAAGCAGAGAATAAATTGACAGAGATTGTCGGTAACTTTTCCGCCCTGATTTTTAACCCAAAGcc                         >  2:1457390/1‑140 (MQ=255)
                                                                                                           gTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGGGTTGGCCTGATGAGCGAAAAAAAAAAAAAACAAAGAGAGAATAAATTGACAGAGATTTTTGCTAACTTTTTCCCCCTGATTTTTTCCCCAATGcc                         >  2:285924/1‑140 (MQ=255)
                                                                                                           gTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGcc                         <  1:1154770/140‑1 (MQ=255)
                                                                                                               tttGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGAGTTTGCATGATGAGCGAAAGGAAAAAGCAAAAAGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTTTTCCCCAAAGCCtgat                     >  2:625553/1‑140 (MQ=255)
                                                                                                                          gAATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa          <  1:453803/140‑1 (MQ=255)
                                                                                                                          aaaTTTTCCCAGACAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa          <  1:704093/139‑1 (MQ=255)
                                                                                                                          aaaTTTTCCCAGAAAAATAAAAAAATTGGAGTTGCCATGATGAGCGAAAATAAAAAGCAACAGCAAGTAAATAAGTTGACAGAGATTGCCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa          <  2:464682/139‑1 (MQ=255)
                                                                                                                                  ccAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa  <  1:625553/140‑1 (MQ=255)
                                                                                                                                  ccAGACAAATAAAAAACCGGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGAAGTGAATAAGTTGACAGAGTTTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa  <  1:100431/140‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                           
TGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAA  >  NC_000913/3206305‑3206574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑