Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,604,3440CT34.1% 119.7 / 43.0 85S62F (TCT→TTT) bglJDNA‑binding transcriptional regulator BglJ
Reads supporting (aligned to +/- strand):  ref base C (15/41);  new base T (29/0);  total (44/41)
Fisher's exact test for biased strand distribution p-value = 5.31e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 103 total aligned reads displayed.

ATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATC  >  NC_000913/4604207‑4604482
                                                                                                                                          |                                                                                                                                          
aTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATtttttttt                                                                                                                                          >  1:853262/1‑140 (MQ=255)
 tGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCACAAAGTTTGCGGGTAACCCTTATAAGCTCCCTACCTATTCCAGTCAGGAGTCATTTCAGGCTGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATtttttcttt                                                                                                                                         <  2:1426089/140‑1 (MQ=255)
   cGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCAtttttttttttt                                                                                                                                       >  1:1192120/1‑140 (MQ=255)
   cGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCAtttttttttttt                                                                                                                                       >  2:763640/1‑140 (MQ=255)
     tCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTttttctttttct                                                                                                                                     <  1:1114675/140‑1 (MQ=255)
     tCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTttttctttttct                                                                                                                                     <  2:1490002/140‑1 (MQ=255)
      cATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGTGGtttttttttttttt                                                                                                                                     >  1:337521/1‑138 (MQ=255)
      cATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCAtttttttttttt                                                                                                                                       >  1:592725/1‑137 (MQ=255)
      cATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATtttttcttttt                                                                                                                                       <  2:592725/137‑1 (MQ=255)
           gtagtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTCTGCCATg                                                                                                                               >  2:489794/1‑140 (MQ=255)
               taTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGaa                                                                                                                           >  1:111021/1‑140 (MQ=255)
                    gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCAtttttttttttt                                                                                                                                       >  2:327756/1‑123 (MQ=255)
                    gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATtttttcttttt                                                                                                                                       <  1:327756/123‑1 (MQ=255)
                    gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTTCCATGAGaaaggag                                                                                                                      >  2:1109596/1‑135 (MQ=255)
                    gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTGCCCTGAGAAGTGAg                                                                                                                      >  1:433601/1‑140 (MQ=255)
                    gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTGCCCTGAGAAGGGAg                                                                                                                      >  1:627790/1‑140 (MQ=255)
                    gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTTCCATGAGAagagag                                                                                                                      >  1:281001/1‑140 (MQ=255)
                    gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAg                                                                                                                          <  2:451938/136‑1 (MQ=255)
                    gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAg                                                                                                                          >  1:451938/1‑136 (MQ=255)
                    gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAg                                                                                                                      <  1:639308/140‑1 (MQ=255)
                      aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCCTTTTTTTTTTTTTTTCCATGGGAAGTGAgcg                                                                                                                    >  2:849240/1‑140 (MQ=255)
                      aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAgcg                                                                                                                    <  1:489794/140‑1 (MQ=255)
                      aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAgcg                                                                                                                    <  2:1192120/140‑1 (MQ=255)
                      aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAgcg                                                                                                                    <  1:1109596/140‑1 (MQ=255)
                       ttGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAgcgc                                                                                                                   >  2:1317357/1‑140 (MQ=255)
                        tGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCa                                                                                                                  <  1:701780/140‑1 (MQ=255)
                         gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTGGCATGAGAAGGGAGCGCag                                                                                                                 >  1:813961/1‑140 (MQ=255)
                         gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTGCCCTGAGAAGGGAGCGCag                                                                                                                 >  2:903895/1‑140 (MQ=255)
                         gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTGCCATGAGAAGTGGGCGCag                                                                                                                 >  1:873718/1‑140 (MQ=255)
                         gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTCTTCCCTGAGAAGTGAgcgccg                                                                                                                 >  1:987962/1‑138 (MQ=255)
                         gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTCTGCCATGAGAAGTGAgcg                                                                                                                    >  1:426861/1‑137 (MQ=255)
                         gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTTTGCCCTGAGAAGGGAGCGCag                                                                                                                 >  2:1337136/1‑140 (MQ=255)
                         gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTTCCATGAGAAGGGAGCGCag                                                                                                                 >  1:891461/1‑140 (MQ=255)
                         gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAgcg                                                                                                                    <  2:426861/137‑1 (MQ=255)
                         gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCag                                                                                                                 >  1:27735/1‑140 (MQ=255)
                          agagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCaga                                                                                                                <  1:1337136/140‑1 (MQ=255)
                          agagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCaga                                                                                                                <  1:873299/140‑1 (MQ=255)
                           gagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGTGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagag                                                                                                               <  1:101438/140‑1 (MQ=255)
                           gagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagag                                                                                                               <  2:647279/140‑1 (MQ=255)
                           gagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagag                                                                                                               <  2:417334/140‑1 (MQ=255)
                           gagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagag                                                                                                               <  2:1099459/140‑1 (MQ=255)
                            agTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTTCCCTGGGAAAGGAgcgcggggg                                                                                                              >  2:892616/1‑135 (MQ=255)
                              tttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagag                                                                                                               >  1:383649/1‑137 (MQ=255)
                              tttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagag                                                                                                               <  2:383649/137‑1 (MQ=255)
                               ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTTTGCCCTGAGAAGGGAGCGCAGAGAggg                                                                                                           >  1:1429633/1‑140 (MQ=255)
                               ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAggg                                                                                                           <  2:139143/140‑1 (MQ=255)
                                             tGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCCTTTTTTTTTTTTTTTCCATGAGAAGTGAGCGCAGAGAGGGGTTATCTTGCctgg                                                                                             >  2:1508373/1‑139 (MQ=255)
                                             tGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCctga                                                                                             <  1:1317357/140‑1 (MQ=255)
                                                       ccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGc                                                                                   <  2:270564/140‑1 (MQ=255)
                                                       ccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGc                                                                                   <  2:574104/140‑1 (MQ=255)
                                                           ataAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTTTGCCATGAGAAGTGAGGGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGCGAtt                                                                               >  1:1258313/1‑140 (MQ=255)
                                                           ataAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGAtt                                                                               <  1:911045/140‑1 (MQ=255)
                                                                cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGtt                                                                          <  2:27735/140‑1 (MQ=255)
                                                                cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGACATTTTTTCTTTTTTTGCCTTGAGGAGTGAATGCCGGGAGGGGTTATCTTGCCTGCCTAAACCGGCGATTTAGtt                                                                          >  2:1285034/1‑140 (MQ=255)
                                                                 tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGAGGGCAGAGAGGGGTTATTTTGCCTGACTGAACTGGCGATTAAGttt                                                                         >  2:911045/1‑140 (MQ=255)
                                                                 tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTTCCATGAGAAGGGAGGGCAGAGAGGGGTTATTTTTCCTGACTGAACTGGGGATTAAGttt                                                                         >  2:205246/1‑140 (MQ=255)
                                                                 tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTGCCCTGAGAAGTGGGGGCAGAGAGGGGTTATCTTTCCTGACTGAACTGGCGGTTAAGttt                                                                         >  2:1409550/1‑140 (MQ=255)
                                                                 tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGGGGGGTTATTTTGCCTGACTAAAATGGCGGTTAAGttt                                                                         >  2:331259/1‑140 (MQ=255)
                                                                 tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGttt                                                                         >  2:114726/1‑140 (MQ=255)
                                                                 tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGCCATTTTTTCTTTTTTTGCCCTTAGAAGAGAGCGCAGAGGGGGGTTATTTTTCCTGACTGAAATGGCGGTTTAGttt                                                                         >  2:1148506/1‑140 (MQ=255)
                                                                  ccATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTc                                                                        <  1:903895/140‑1 (MQ=255)
                                                                          ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTTCCATGGGAAGTGGGCGCAGAGAGGGGTTATCTTGTCTGACTTGAATGGCGGTTAAGGTTTCGCGGAcc                                                                >  2:747462/1‑140 (MQ=255)
                                                                          ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTGCCCTGAGAAGGGAGCGCAGAGAGGGGGTATCTTTCCTGACTGAACTGGCGATTTAGTTTTCGCGGAcc                                                                >  2:28188/1‑140 (MQ=255)
                                                                          ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTAcc                                                                <  1:899651/140‑1 (MQ=255)
                                                                          ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCAGACTGAACTGGCGATTAAGTTTCCGCGTAcc                                                                <  2:299417/140‑1 (MQ=255)
                                                                          agacaggTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTAcc                                                                <  1:1135658/134‑1 (MQ=255)
                                                                             ccAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTTTGCCTTTGGGAGTGAGAGCAGGGAGGGGTTATTTTGCCTTACTGAATTCGGGATTAAATTTCCGCGTACCCgc                                                             >  2:1304061/1‑139 (MQ=255)
                                                                                gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCCTTTTTTTTTTTTTTGCCGTGGGGAGGGAGTGCCCGGAGGGGATTTTTTGGCTGACTGAATCGGAGATTTAGTTTTCGCGGCCCCGGGGt                                                          >  2:446703/1‑140 (MQ=255)
                                                                                gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTGCCATGAGAAGGGAGCGCAGGGGGGGGTTATCTTTCCTGACTGAAATGGGGATTAAGTTTTCGCGGACCCGGCgg                                                          >  2:1439830/1‑139 (MQ=255)
                                                                                gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTCTTCCCTGGGGAGGGAGGGCAGGGAGGGGTTATCTTTTCTGACTGTAATGGCGGGTAAGGTTTCGCGGATCCGGCgg                                                          >  2:953361/1‑139 (MQ=255)
                                                                                gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTTTGCCCTGAGAAGGGAGCGCAGAGAGGGATTATTTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCcgcgg                                                          >  2:158765/1‑139 (MQ=255)
                                                                                     gAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTTTTCCATGAGAAGGGAGCGCAGAGAGGGGTTTTCTTTCCTGACTGAACTGGCGATTAAGTTTCCGCGTGCCCGGCGTTTTGt                                                     >  2:1248673/1‑140 (MQ=255)
                                                                                      aGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATTTTTCCTGACTGAACTGGCGGTTAAATTTCCGCGTACCCCGCGGTTTGtt                                                    >  2:1234443/1‑140 (MQ=255)
                                                                                        tCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTAt                                                  <  2:28125/140‑1 (MQ=255)
                                                                                          aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTg                                                <  1:212442/140‑1 (MQ=255)
                                                                                           tttCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGAGGGCAGGGAGGGGTTATCTTTCCTGACTGAACTGGCGGTTAAGTTTTCGCGTACCCCGCGGTTAGtctgtct                                               >  2:1135658/1‑134 (MQ=255)
                                                                                             tCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTGCCATGAGAAGGGAGCGCAGAGGGGGGTTTTCTTTTCTGACTGAACTGGCGATTAAGTTTTCGCGTACCCCGCGGTTTGTTATTGCgg                                             >  2:1290097/1‑140 (MQ=255)
                                                                                               aGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGAGGGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGGTTAAGTTTTCGCGTACCCGGCGGTTTGTTTTTgggggg                                           >  2:945431/1‑138 (MQ=255)
                                                                                               aGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgat                                           <  1:953361/140‑1 (MQ=255)
                                                                                               aGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGGCATTTTTTCTTTTTCTGCCCTTAGGAGGGAGCGCAAAGAGGGAGTTTATTGCCTGAGTGAAATGGCGATTAAAAGTCCCCGCATCCCGCGTTTTGTTATTGCggcg                                           >  2:466955/1‑138 (MQ=255)
                                                                                                  cagCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgat                                        <  1:1192754/138‑1 (MQ=255)
                                                                                                  aTGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgat                                        <  1:531137/140‑1 (MQ=255)
                                                                                                  aTGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgat                                        <  1:28188/140‑1 (MQ=255)
                                                                                                  aTGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgat                                        <  2:750886/140‑1 (MQ=255)
                                                                                                   tGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgatg                                       >  1:1359132/1‑140 (MQ=255)
                                                                                                   tGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgatg                                       <  1:1291843/140‑1 (MQ=255)
                                                                                                       aTGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGatat                                   <  1:114726/140‑1 (MQ=255)
                                                                                                       aTGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGatat                                   <  2:111021/140‑1 (MQ=255)
                                                                                                            gcgGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAg                              <  1:1234443/140‑1 (MQ=255)
                                                                                                              ggATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTCTGCCATGAGAAGGGAGCGCAGAGAGGGAGTATCTTGCCTGACTGAACTGGCGGTTAAGTTTCCGCGTACCCCGCGTTTTGTTATTGCGGATGATGATATTTAAGCt                            >  2:236580/1‑140 (MQ=255)
                                                                                                              ggATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCt                            <  1:158765/140‑1 (MQ=255)
                                                                                                                aTCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTCTGCCCTGAGAAGTGAGCGCAGAGAGGGGTTATCTTTCCTGACTTAACTGGCGATTAAGTTTTCGCGTACCCCGCGTTTTGTTATTTCGGATGATGATATTTAAGcctg                          >  2:1192754/1‑137 (MQ=255)
                                                                                                                          cggc‑ggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGt                <  2:337521/140‑1 (MQ=255)
                                                                                                                             c‑ggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCa             <  1:981574/140‑1 (MQ=255)
                                                                                                                                gTCATTTTTTTTTTTTTTTCCATGAGAAGGGAGCGCAGAGAGGGGTTTTCTTTCCTGACTGAACTGGCGGTTAAGTTTTCGCGGACCCGGCGTTTAGTTTTTGCGGGTGATGATATTGAAGCTCGGCTGGTTGGTTCAtt           >  2:1025764/1‑140 (MQ=255)
                                                                                                                                gTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCAtt           <  2:908186/140‑1 (MQ=255)
                                                                                                                                        ttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAt   <  1:1508373/140‑1 (MQ=255)
                                                                                                                                        ttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAt   <  1:1439830/140‑1 (MQ=255)
                                                                                                                                        ttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAt   <  2:710059/140‑1 (MQ=255)
                                                                                                                                         tctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATc  <  2:407806/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                          
ATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATC  >  NC_000913/4604207‑4604482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑