| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,628,649 | 0 | A | C | 24.4% | 93.7 / 13.6 | 45 | intergenic (+102/+206) | nadR/ettA | DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR/energy‑dependent translational throttle protein EttA |
| Reads supporting (aligned to +/- strand): ref base A (31/3); new base C (0/11); total (31/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.59e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCT‑CTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAA‑CCTGCACGGGTGACCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACAGTTTTACA > NC_000913/4628514‑4628788 | aCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCT‑CTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAA‑CCTg < 2:821021/140‑1 (MQ=255)aCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGTGATGAAACTACTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCT‑CTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAA‑CCTg < 2:766756/140‑1 (MQ=255) gTGCGGGAGATGATGGGGGAGCAGAGATAACCATGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCT‑CTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAA‑CCTGCACg > 1:1404864/1‑140 (MQ=255) ggAGATGATGGGGGAGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCT‑CTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAA‑CCTGCACGGGTGa > 1:1268919/1‑140 (MQ=255) gatgGGGGAGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCT‑CTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAA‑CCTGCACGGGTGACCCGcc > 1:122599/1‑140 (MQ=255) aaCCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCT‑CTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAC‑CCTGCACGGGTGACCCGCCACGAAATTGAGGATGCt < 1:146103/140‑1 (MQ=255) cGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCT‑CTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAA‑CCTGCACGGGTGACCCGCCACGAAATTGAGGATGCTAAg > 2:931947/1‑140 (MQ=255) gTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTAGAAACCCT‑CTGTGGAAGAGGCTGTCGAGATAAAAAAGGGTTTGAAAAAA‑CCTGCGCGGGTGCCCCGCCACGAAATTGAGGATGCTAAgg < 2:1278927/140‑1 (MQ=255) gatgaAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCT‑CTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAA‑CCTGCACGGGTGACCCGCCACGAAATTGAGGATGCTAAGGCt > 1:678299/1‑140 (MQ=255) gatgaAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCT‑CTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAA‑CCTGCACGGGTGAACCGCCACGAAATTGAg > 2:405976/1‑128 (MQ=255) gatgaAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCT‑CTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAA‑CCTGCACGGGTGAACCGCCACGAAATTGAg < 1:405976/128‑1 (MQ=255) aaCTGCTCAAAGGGGAGGTATAAAATGATTTTTTTTGATGAGTTAAAACCCT‑CTCGGGAAGGGGCTGTCGAGATAAAACAGGGTTTGAAAAAC‑CCTGCAGGGGTGCCCCGCCACGAAATTGAGGATGCTAAGGCtgttgt < 2:1404864/140‑1 (MQ=255) cTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCT‑CTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAA‑CCTGCACGGGTGACCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAg > 1:714415/1‑140 (MQ=255) gCTAAAAGGGGAGGTAAAAAATGATTTTTTTTGATGAGTTAAAAACCC‑CTCGGAAGGAGGTTGTCGAGATTAAACAAGGTTTGAAAAAC‑CCTGCCCGGGTGACCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGAc < 2:793294/140‑1 (MQ=255) cTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCT‑CTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAA‑CCTGCACGGGTGACCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGAcc > 2:1148322/1‑140 (MQ=255) aaGGGGAGGTATAAAATGAGTTTTTTTGATGAGTTAAAACCCT‑CTCTGGAAGAGGCTGTCGAAATAAAACAAGGTTTAAAAAAC‑CCTGCCCGGGGGCCCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGaa < 2:1049175/140‑1 (MQ=255) cGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCT‑CTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAA‑CCTGCACGGGTGACCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACgg > 2:1140418/1‑140 (MQ=255) aGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCT‑CTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAA‑CCTGCACGGGCGACCCGCCACGAAATCGAGGTTGCGAAGGTTGATGTAGACCGGAAACGgtg > 1:1131296/1‑140 (MQ=255) aaaaTGATTTTTTTTGATGAGTTAAAACCCT‑CTCGGGAAGAGGCTGTCGAGATAAAACAGGGTTTAAAAAAC‑CCTGCGGGGGTGACCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCAcg < 2:283830/140‑1 (MQ=255) aaaTGAGTTTTTTTGATGAGTTGAAAACCT‑CTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAA‑CCTGCACGGGTGACCCGCCACGAAATTGAGGATGCTAAGGCTGTTGGAGACCGGAAACGGTGTTCAcgc > 2:1350011/1‑140 (MQ=255) tttttttttGATGAGTTGAAACCCT‑TTCGGGAAGAGGCTGTGGAGATAAAACAGGGTTTAAAAAAC‑CCTGCCGGGGTGCCCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCAt < 2:1162319/138‑1 (MQ=255) aGTTTTTTTGATGAGTTGAAAACCT‑CTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAA‑CCTGCACGGGTGACCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCAt > 2:930366/1‑140 (MQ=255) aGTTTTTTTGATGAGTTGAAAACCT‑CTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAA‑CCTGCACGGGTGACCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCAt > 2:807078/1‑140 (MQ=255) gTTTTTTTGATGAGTTGAAAACCT‑CTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAA‑CCTGCACGGGTGACCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATc > 2:593162/1‑140 (MQ=255) gTTTTTTTGATGAGTTGAAAACCT‑CTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAA‑CCTGCACGGGTGACCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATc > 2:1086052/1‑140 (MQ=255) gTTTTTTTGATGAGTTGAAAACCT‑CTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAA‑CCTGCACGGGTGACCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATc > 1:779132/1‑140 (MQ=255) gTTTTTTTGATGAGTTGAAAACCT‑CTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAA‑CCTGCACGGGTGACCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATc > 1:490077/1‑140 (MQ=255) gTTTTTTTGATGAGTTGAAAACCT‑CTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAA‑CCTGCACGGGTGACCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATc > 1:476820/1‑140 (MQ=255) tgatgaTTTAAAACCCT‑CTCGGGAAGGGGCTGTAGAGATTAAACAAGGTTTAAAAAAC‑CCTGCAGGGGTGCCCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCAtt < 2:115167/140‑1 (MQ=255) atgaGTTAAAACCCT‑CTCTGGAAGAGGCTGTCGAGATAAAACAAGGTTTAAAAAAC‑CCGGCAGGGGTGACCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCg < 2:779132/140‑1 (MQ=255) atgaGTTAAAACCCT‑CTCGGGAAGGGGCTGTCGAGATAAAAAAAGTTTTAAAAAAC‑CCTGCAGGGGTGACCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCg < 2:490077/140‑1 (MQ=255) atgaGTT‑AAAACCTCCTGGGGAAGAGGCTGTCGAAATAAAACAAGGTTTAAAAAAC‑CCTGCCCGGGTGCCCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCg < 2:476820/140‑1 (MQ=255) gggtttaaaacCCT‑CTCGGGAAGGGGCTGTCGAGATAAAACAAGTTTTAAAAAAC‑CCTGCGCGGGTGACCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCgg < 2:678299/133‑1 (MQ=255) ttGAAAACCT‑CTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAA‑CCTGCACGGGTGACCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCt > 1:115167/1‑140 (MQ=255) ctctGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAA‑CCTGCACGGGTGACCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGa > 1:1278046/1‑140 (MQ=255) ctctGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAA‑CCTGCACGGGTGACCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGa > 1:168461/1‑140 (MQ=255) ctctGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAA‑CCTGCACGGGTGACCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGa > 1:439250/1‑140 (MQ=255) tGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAA‑CCTGCACGGGTGACCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTgtctc > 1:1442899/1‑136 (MQ=255) gCTGTCGAGATTAAACAAGGTTTGAAAAAA‑CCTGCACGGGTGACCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTa > 1:1390893/1‑140 (MQ=255) tGTCGAGATTAAACAGGGTTTAAAAAAC‑CCTGCCGGGGTGCCCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTAcc < 1:1148322/140‑1 (MQ=38) tCGAGATTAAACAAGGTTTGAAAAAA‑CCTGCACGGGTGACCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACcgc > 1:281433/1‑140 (MQ=255) tCGAGATTAAACAAGGTTTGAAAAAA‑CCTGCACGGGTGACCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACcgc > 2:587526/1‑140 (MQ=255) aaaagaTTAAACAAGTTTTAAAAAAC‑CCTGCCGGGGTGCCCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACcgc < 2:1329661/137‑1 (MQ=38) gggaTTAACCAAGTTTTAAAAAAA‑CCGCGGGGGGTGTCCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCGCGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGt < 1:154291/138‑1 (MQ=37) gagaTAAAACAGGGTTTAAAAAAC‑CCGGCCGGGGGGCCCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGt < 2:122599/140‑1 (MQ=37) gaaataaaaCAGGGTTTAAAAAAC‑CCTGCGGGGGTGCCCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGt < 1:746762/134‑1 (MQ=37) aaaatAAAAAAAGTTTAAAAAAACCCGGCAGGGGTTCCCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGt < 2:281433/136‑1 (MQ=37) aaaaCAAGTTTAAAAAAAC‑CCGGCGGGGGTGACCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTAt < 2:439250/139‑1 (MQ=37) aaaaaaGGTTTAAAAAAA‑CCTGCGCGGGGGACCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATc < 2:507715/140‑1 (MQ=255) aaacaGGTTTAAAAAAACCCTGCCGGGGGGCCCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATc < 2:1278046/136‑1 (MQ=37) gctttcgAAAAAA‑CCTCCTGGCGGGTCCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGcc < 2:714415/134‑1 (MQ=25) aGGTTAAAAAAAC‑CCTGCGCGGGTGACCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGcc < 1:544232/140‑1 (MQ=255) ttttAAAAAAC‑CCTGCGGGGGTGACCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTa < 1:580248/139‑1 (MQ=255) gTTTGAAAAAA‑CCTGCACGGGTGACCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTa > 1:590486/1‑140 (MQ=255) gTTTAAAAAAC‑CCTGCCGGGGTGCCCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTa < 1:210318/140‑1 (MQ=37) tttGAAAAAA‑CCTGCACGGGTGACCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGctgtc > 1:1329661/1‑136 (MQ=255) tGAAAAAA‑CCTGCACGGGTGACCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACAg > 2:823114/1‑140 (MQ=255) gAAAAAA‑CCTGCACGGGTGACCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACAGt > 2:84829/1‑140 (MQ=255) gAAAAAA‑CCTGCACGGGTGACCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACAGt > 2:1528753/1‑140 (MQ=255) a‑CCTGCACGGGTGCCCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACAGTTTTACa < 1:84829/140‑1 (MQ=255) a‑CCTGCACGGGTGACCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACAGtctgtct > 1:203441/1‑134 (MQ=255) | ACTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCT‑CTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAA‑CCTGCACGGGTGACCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACAGTTTTACA > NC_000913/4628514‑4628788 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |