Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,902,147	A→T	I34F (ATC→TTC)	manX →	mannose‑specific PTS enzyme IIAB component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,902,147	0	A	T	100.0%	344.1 / NA	97	I34F (ATC→TTC)	manX	mannose‑specific PTS enzyme IIAB component
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (52/45);  total (52/45)

CGTTGAGGTGTTAACGATAATAAAGGAGGTAGCAAGTGACCATTGCTATTGTTATAGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGATCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTGGCAAAACTCGACACCACTAAAGGCGTGCTGTTTCTCGTTGATACATGGGGAGGCAGCCCGTTCAATGCTGCCAGC  >  NC_000913/1902013‑1902281                                                                                                                                       |                                                                                                                                       cGTTGAGGTGTTAACGATAATAAAGGAGGTAGCAAGTGACCATTGCTATTGTTATAGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGAt                                                                                                                                   <  2:76136/140‑1 (MQ=255)   ttGAGGTGTTAACGATAATAAAGGAGGTAGCAAGTGACCATTGCTATTGTTATAGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGAttt                                                                                                                                 >  2:111016/1‑140 (MQ=255)   ttGAGGTGTTAACGATAATAAAGGAGGTAGCAAGTGACCATTGCTATTGTTATAGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGAttt                                                                                                                                 >  2:1206328/1‑140 (MQ=255)     gAGGTGTTAACGATAATAAAGGAGGTAGCAAGTGACCATTGCTATTGTTATAGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCg                                                                                                                               >  2:1497408/1‑140 (MQ=255)       ggTGTTAACGATAATAAAGGAGGTAGCAAGTGACCATTGCTATTGTTATAGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGtt                                                                                                                             <  1:1351620/140‑1 (MQ=255)         tgtTAACGATAATAAAGGAGGTAGCAAGTGACCATTGCTATTGTTATAGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTcc                                                                                                                           <  2:715126/140‑1 (MQ=255)           ttAACGATAATAAAGGAGGTAGCAAGTGACCATTGCTATTGTTATAGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCa                                                                                                                          >  1:1166168/1‑139 (MQ=255)           ttAACGATAATAAAGGAGGTAGCAAGTGACCATTGCTATTGTTATAGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCa                                                                                                                          <  2:1166168/139‑1 (MQ=255)           ttAACGATAATAAAGGAGGTAGCAAGTGACCATTGCTATTGTTATAGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAg                                                                                                                         >  1:1240494/1‑140 (MQ=255)             aaCGATAATAAAGGAGGTAGCAAGTGACCATTGCTATTGTTATAGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCa                                                                                                                          >  2:1115564/1‑137 (MQ=255)             aaCGATAATAAAGGAGGTAGCAAGTGACCATTGCTATTGTTATAGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCa                                                                                                                          <  1:1115564/137‑1 (MQ=255)             aaCGATAATAAAGGAGGTAGCAAGTGACCATTGCTATTGTTATAGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGt                                                                                                                       >  1:881478/1‑140 (MQ=255)                gATAATAAAGGAGGTAGCAAGTGACCATTGCTATTGTTATAGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGaa                                                                                                                    >  1:648122/1‑140 (MQ=255)                 ataataAAGGAGGTAGCAAGTGACCATTGCTATTGTTATAGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGaaa                                                                                                                   <  2:538033/140‑1 (MQ=255)                  taataaAGGAGGTAGCAAGTGACCATTGCTATTGTTATAGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGaaaa                                                                                                                  >  2:958818/1‑140 (MQ=255)                     taaAGGAGGTAGCAAGTGACCATTGCTATTGTTATAGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGc                                                                                                               >  2:226456/1‑140 (MQ=255)                     taaAGGAGGTAGCAAGTGACCATTGCTATTGTTATAGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGc                                                                                                               >  1:832310/1‑140 (MQ=255)                     taaAGGAGGTAGCAAGTGACCATTGCTATTGTTATAGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGc                                                                                                               >  1:1482403/1‑140 (MQ=255)                     taaAGGAGGTAGCAAGTGACCATTGCTATTGTTATAGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGc                                                                                                               >  2:904695/1‑140 (MQ=255)                        aggaggTAGCAAGTGACCATTGCTATTGTTATAGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAACTGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGa                                                                                                            <  2:881478/140‑1 (MQ=255)                        aggaggTAGCAAGTGACCATTGCTATTGTTATAGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGa                                                                                                            >  2:53595/1‑140 (MQ=255)                          gaggTAGCAAGTGACCATTGCTATTGTTATAGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGaaa                                                                                                          >  2:137553/1‑140 (MQ=255)                            ggTAGCAAGTGACCATTGCTATTGTTATAGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACg                                                                                                        >  1:1341461/1‑140 (MQ=255)                            ggTAGCAAGTGACCATTGCTATTGTTATAGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACg                                                                                                        >  2:1266248/1‑140 (MQ=255)                             gTAGCAAGTGACCATTGCTATTGTTATAGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATTCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGa                                                                                                            >  1:1121782/1‑135 (MQ=255)                             gTAGCAAGTGACCATTGCTATTGTTATAGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATTCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGa                                                                                                            <  2:1121782/135‑1 (MQ=255)                             gTAGCAAGTGACCATTGCTATTGTTATAGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGc                                                                                                       >  2:842918/1‑140 (MQ=255)                                gCAAGTGACCATTGCTATTGTTATAGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGa                                                                                                    <  1:842918/140‑1 (MQ=255)                                gCAAGTGACCATTGCTATTGTTATAGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGa                                                                                                    <  2:338720/140‑1 (MQ=255)                                  aaGTGACCATTGCTATTGTTATAGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGAtt                                                                                                  <  2:478226/140‑1 (MQ=255)                                  aaGTGACCATTGCTATTGTTATAGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGAtt                                                                                                  <  1:629875/140‑1 (MQ=255)                                    gTGACCATTGCTATTGTTATAGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCt                                                                                                      >  1:279686/1‑134 (MQ=255)                                    gTGACCATTGCTATTGTTATAGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCt                                                                                                      <  2:279686/134‑1 (MQ=255)                                    gTGACCATTGCTATTGTTATAGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGa                                                                                                >  1:1365638/1‑140 (MQ=255)                                    gTGACCATTGCTATTGTTATAGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGa                                                                                                >  1:295777/1‑140 (MQ=255)                                    gTGACCATTGCTATTGTTATAGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGa                                                                                                >  2:1040197/1‑140 (MQ=255)                                          aTTGCTATTGTTATAGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTa                                                                                          <  2:942600/140‑1 (MQ=255)                                          aTTGCTATTGTTATAGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTa                                                                                          <  2:1240494/140‑1 (MQ=255)                                           ttGCTATTGTTATAGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCTAAACGCTGATTGAAAAGTAc                                                                                         >  1:1464574/1‑140 (MQ=255)                                           ttGCTATTGTTATAGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCTAAACGCTGATTGAAAAGTAc                                                                                         <  2:1464574/140‑1 (MQ=255)                                            tGCTATTGTTATAGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACa                                                                                        >  1:1432346/1‑140 (MQ=255)                                              cTATTGTTATAGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAAc                                                                                      <  2:327117/140‑1 (MQ=255)                                                aTTGTTATAGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGc                                                                                    >  2:1398044/1‑140 (MQ=255)                                                 ttGTTATAGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCt                                                                                   <  1:1087189/140‑1 (MQ=255)                                                     tataGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGt                                                                               <  1:1040197/140‑1 (MQ=255)                                                      ataGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGtt                                                                              >  1:495022/1‑140 (MQ=255)                                                      ataGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGtt                                                                              >  1:261450/1‑140 (MQ=255)                                                        aGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTgg                                                                            <  2:295777/140‑1 (MQ=255)                                                         ggCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTGGc                                                                           >  1:348441/1‑140 (MQ=255)                                                           cacacaTGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTGGCaa                                                                         >  1:755515/1‑140 (MQ=255)                                                             cacaTGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTGGCaaaa                                                                       <  1:240732/140‑1 (MQ=255)                                                              acaTGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCt                                                                                   <  1:892543/127‑1 (MQ=255)                                                              acaTGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCt                                                                                   >  2:892543/1‑127 (MQ=255)                                                              acaTGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTGGCAAAAc                                                                      >  2:819600/1‑140 (MQ=255)                                                              acaTGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTGGCAAAAc                                                                      >  2:294384/1‑140 (MQ=255)                                                               caTGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTGGCAAAACt                                                                     <  1:1224270/140‑1 (MQ=255)                                                               caTGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTGGCAAAACt                                                                     <  2:648122/140‑1 (MQ=255)                                                                  ggTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTGGCAAAACTCGa                                                                  <  2:1432346/140‑1 (MQ=255)                                                                   gTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTGGCAAAACTCGac                                                                 <  2:257183/140‑1 (MQ=255)                                                                   gTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTGGCAAAACTCGac                                                                 <  2:1365638/140‑1 (MQ=255)                                                                   gTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGCTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTGGCAAAACTCGac                                                                 <  2:522486/140‑1 (MQ=255)                                                                    ttGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTGGCAAAACTCGac                                                                 >  1:502309/1‑139 (MQ=255)                                                                    ttGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTGGCAAAACTCGac                                                                 <  2:502309/139‑1 (MQ=255)                                                                       ggCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTGGCAAAACTCGAcacca                                                             >  1:82113/1‑140 (MQ=255)                                                                       ggCTGCAGAGCAGTTGCTTAAAACGGAAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTGGCAAAACTCGac                                                                 >  2:1174292/1‑136 (MQ=255)                                                                       ggCTGCAGAGCAGTTGCTTAAAACGGAAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTGGCAAAACTCGac                                                                 <  1:1174292/136‑1 (MQ=255)                                                                        gCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCGGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTGGCAAAACTCGAcaccac                                                            >  2:337091/1‑140 (MQ=255)                                                                          tGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTGGCAAAACTCGACACCACTa                                                          >  1:1058167/1‑140 (MQ=255)                                                                          tGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTGGCAAAACTCGACACCACTa                                                          <  1:904695/140‑1 (MQ=255)                                                                            cAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTGGCAAAACTCGACACCACTaaa                                                        >  2:1230046/1‑140 (MQ=255)                                                                            cAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTGGCAAAACTCGACACCACTaaa                                                        >  2:1193751/1‑140 (MQ=255)                                                                            cAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTGGCAAAACTCGACACCACTaaa                                                        >  2:1044372/1‑140 (MQ=255)                                                                            cAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTGGCAAAACTCGACACCACTaaa                                                        >  1:636246/1‑140 (MQ=255)                                                                              gagCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTGGCAAAACTCGACACCACTAAAgg                                                      >  2:996133/1‑140 (MQ=255)                                                                                gCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTGGCAAAACTCGACACCACTAAAGGCg                                                    <  2:348441/140‑1 (MQ=255)                                                                                   gTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTGGCAAAACTCGACACCACTAAAGGCGTGc                                                 <  2:1178098/140‑1 (MQ=255)                                                                                      gCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTGGCAAAACTCGACACCACTAAAGGCGTGCTGt                                              >  2:332706/1‑140 (MQ=255)                                                                                      gCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTGGCAAAACTCGACACCACTAAAGGCGTGCTGt                                              >  1:1219097/1‑140 (MQ=255)                                                                                        ttAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTGGCAAAACTCGACACCGCTAAAGGCGTGCTGttt                                            <  1:1266248/140‑1 (MQ=255)                                                                                        ttAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTGGCAAAACTCGACACCACTAAAGGCGTGCTGttt                                            <  1:111016/140‑1 (MQ=255)                                                                                          aaaaCGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTGGCAAAACTCGACACCACTAAAGGCGTGCTGTTtct                                          <  1:1193751/140‑1 (MQ=255)                                                                                          aaaaCGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTGGCAAAACTCGACACCACTAAAGGCGTGCTGTTtct                                          <  2:636246/140‑1 (MQ=255)                                                                                            aaCGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTGGCAAAACTCGACACCACTAAAGGCGTGCTGTTTCTCg                                        <  1:192121/140‑1 (MQ=255)                                                                                             aCGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTGGCAAAACTCGACACCACTAAAGGCGTGCTGTTTCTCGt                                       <  1:1206328/140‑1 (MQ=255)                                                                                                gCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTGGCAAAACTCGACACCACTAAAGGCGTGCTGTTTCTCGTTGa                                    <  2:755515/140‑1 (MQ=255)                                                                                                 cAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTGGCAAAACTCGACACCACTAAAGGCGTGCTGTTTCTCGTTGAt                                   >  2:773996/1‑140 (MQ=255)                                                                                                 cAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTGGCAAAACTCGACACCACTAAAGGCGTGCTGTTTCTCGTTGAt                                   >  1:1260693/1‑140 (MQ=255)                                                                                                 cAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTGGCAAAACTCGACACCACTAAAGGCGTGCTGTTTCTCGTTGAt                                   >  1:643542/1‑140 (MQ=255)                                                                                                  aGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTGGCAAAACTCGACACCACTAAAGGCGTGCTGTTTCTCGTTGATa                                  <  2:12731/140‑1 (MQ=255)                                                                                                               ggCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTGGCAAAACTCGACACCACTAAAGGCGTGCTGTTTCTCGTTGATACATGGGGAGGCAg                     <  1:137553/140‑1 (MQ=255)                                                                                                                gCGAGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTGGCAAAACTCGACACCACTAAAGGCGTGCTGTTTCTCGTTGATACATGGGGAGGCAGc                    <  1:1081826/140‑1 (MQ=255)                                                                                                                   aGCAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTGGCAAAACTCGACACCACTAAAGGCGTGCTGTTTCTCGTTGATACATGGGGAGGCAGCCCg                 >  2:1329146/1‑140 (MQ=255)                                                                                                                     cAGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTGGCAAAACTCGACACCACTAAAGGCGTGCTGTTTCTCGTTGATACATGGGGAGGCAGCCCGtt               <  1:53595/140‑1 (MQ=255)                                                                                                                      aGGAAAACGTCGGCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTGGCAAAACTCGACACCACTAAAGGCGTGCTGTTTCTCGTTGATACATGGGGAGGCAGCCCGTTc              >  1:177737/1‑140 (MQ=255)                                                                                                                              gTCGGCTGGTTCGATTTCGTTCTAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTGGCAAAACTCGACACCACTAAAGGCGTGCTGTTTCTCGTTGATACATGGGGAGGCAGCCCGTTCAAtgctgc      >  1:600420/1‑140 (MQ=255)                                                                                                                                  gCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTGGCAAAACTCGACACCACTAAAGGCGTGCTGTTTCTCGTTGATACATGGGGAGGCAGCCCGTTCAATGCTGCCAGc  <  2:832310/140‑1 (MQ=255)                                                                                                                                  gCTGGTTCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTGGCAAAACTCGACACCACTAAAGGCGTGCTGTTTCTCGTTGATACATGGGGAGGCAGCCCGTTCAATGCTGCCAGc  >  2:876496/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                       CGTTGAGGTGTTAACGATAATAAAGGAGGTAGCAAGTGACCATTGCTATTGTTATAGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGAGCAGGAAAACGTCGGCTGGATCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTGGCAAAACTCGACACCACTAAAGGCGTGCTGTTTCTCGTTGATACATGGGGAGGCAGCCCGTTCAATGCTGCCAGC  >  NC_000913/1902013‑1902281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑