Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,645,581	A→C	L18R (CTG→CGG)	relE ←	Qin prophage; mRNA interferase toxin RelE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,645,581	0	A	C	100.0%	331.4 / NA	84	L18R (CTG→CGG)	relE	Qin prophage; mRNA interferase toxin RelE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (42/42);  total (42/42)

AAGGCGATAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCAGGCATACCACGGAGCTTGTTTGCTTCAATCCGGGGTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCAGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGCTTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTTCA  >  NC_000913/1645448‑1645714                                                                                                                                      |                                                                                                                                      aaGGCGATAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCAGGCATACCACGGAGCTTGTTTGCTTCAATCCGGGGTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTc                                                                                                                                 >  2:260691/1‑140 (MQ=255) aaGGCGATAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCAGGCATACCACGGAGCTTGTTTGCTTCAATCCGGGGTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTc                                                                                                                                 >  1:687667/1‑140 (MQ=255) aaGGCGATAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCAGGCATACCACGGAGCTTGTTTGCTTCAATCCGGGGTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTc                                                                                                                                 >  1:237482/1‑140 (MQ=255)   ggCGATAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCAGGCATACCACGGAGCTTGTTTGCTTCAATCCGGGGTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGc                                                                                                                               <  2:580079/140‑1 (MQ=255)   ggCGATAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCAGGCATACCACGGAGCTTGTTTGCTTCAATCCGGGGTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGc                                                                                                                               >  1:727477/1‑140 (MQ=255)     cGATAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCAGGCATACCACGGAGCTTGTTTGCTTCAATCCGGGGTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCa                                                                                                                             <  2:35/140‑1 (MQ=255)     cGATAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCAGGCATACCACGGAGCTTGTTTGCTTCAATCCGGGGTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCa                                                                                                                             <  2:876106/140‑1 (MQ=255)      gATAGCCTGAAGACCGGTGCTTAATCTTGTAACAATCAGGCATACCACGGAGCTTGTTTGCTTCAATCCGGGGGGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCAt                                                                                                                            >  2:653059/1‑140 (MQ=255)      gATAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCAGGCATACCACGGAGCTTGTTTGCTTCAATCCGGGGTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCAt                                                                                                                            >  1:751990/1‑140 (MQ=255)        tAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCAGGCATACCACGGAGCTTGTTTGCTTCAATCCGGGGTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTc                                                                                                                          >  2:384678/1‑140 (MQ=255)        tAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCAGGCATACCACGGAGCTTGTTTGCTTCAATCCGGGGTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTc                                                                                                                          >  2:242397/1‑140 (MQ=255)        tAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCAGGCATACCACGGAGCTTGTTTGCTTCAATCCGGGGTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTc                                                                                                                          >  1:476929/1‑140 (MQ=255)              gAAGACCGGAGCTTAATCTTGTAACAATCAGGCATACCACGGAGCTTGTTTGCTTCAATCCGGGGTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAg                                                                                                                    >  1:704000/1‑140 (MQ=255)               aaGACCGGAGCTTAATCTTGTAACAATCAGGCATACCACGGAGCTTGTTTGCTTCAATCCGGGGTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGt                                                                                                                   >  2:813912/1‑140 (MQ=255)               aaGACCGGAGCTTAATCTTGTAACAATCAGGCATACCACGGAGCTTGTTTGCTTCAATCCGGGGTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGt                                                                                                                   >  2:66624/1‑140 (MQ=255)               aaGACCGGAGCTTAATCTTGTAACAATCAGGCATACCACGGAGCTTGTTTGCTTCAATCCGGGGTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGt                                                                                                                   >  1:202363/1‑140 (MQ=255)               aaGACCGGAGCTTAATCTTGTAACAATCAGGCATACCACGGAGCTTGTTTGCTTCAATCCGGGGTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGt                                                                                                                   >  1:1080833/1‑140 (MQ=255)                aGACCGGAGCTTAATCTTGTAACAATCAGGCATACCACGGAGCTTGTTTGCTTCAATCCGGGGTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTg                                                                                                                  <  2:900782/140‑1 (MQ=255)                    cGGAGCTTAATCTTGTAACAATCAGGCATACCACGGAGCTTGTTTGCTTCAATCCGGGGTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCg                                                                                                              <  2:476929/140‑1 (MQ=255)                     ggAGCTTAATCTTGTAACAATCAGGCATACCACGGAGCTTGTTTGCTTCAATCCGGGGTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGc                                                                                                             >  1:917589/1‑140 (MQ=255)                      gAGCTTAATCTTGTAACAATCAGGCATACCACGGAGCTTGTTTGCTTCAATCCGGGGTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCt                                                                                                            >  2:242513/1‑140 (MQ=255)                         cTTAATCTTGTAACAATCAGGCATACCACGGAGCTTGTTTGCTTCAATCCGGGGTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGt                                                                                                         >  2:515648/1‑140 (MQ=255)                           tAATCTTGTAACAATCAGGCATACCACGGAGCTTGTTTGCTTCAATCCGGGGTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCa                                                                                                       <  2:814484/140‑1 (MQ=255)                            aaTCTTGTAACAATCAGGCATACCACGGAGCTTGTTTGCTTCAATCCGGGGTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCaa                                                                                                      >  1:1290877/1‑140 (MQ=255)                             aTCTTGTAACAATCAGGCATACCACGGAGCTTGTTTGCTTCAATCCGGGGTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCaaa                                                                                                     <  2:704000/140‑1 (MQ=255)                              tCTTGTAACAATCAGGCATACCACGGAGCTTGTTTGCTTCAATCCGGGGTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCaaaa                                                                                                    >  1:1098758/1‑140 (MQ=255)                                   tAACAATCAGGCATACCACGGAGCTTGTTTGCTTCAATCCGGGGTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAg                                                                                               >  1:734372/1‑140 (MQ=255)                                        aTCAGGCATACCACGGAGCTTGTTTGCTTCAATCCGGGGTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAAt                                                                                          >  1:242109/1‑140 (MQ=255)                                           aGGCATACCACGGAGCTTGTTTGCTTCAATCCGGGGTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACg                                                                                       <  2:751990/140‑1 (MQ=255)                                            ggCATACCACGGAGCTTGTTTGCTTCAATCCGGGGTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGc                                                                                      <  1:1264803/140‑1 (MQ=255)                                            ggCATACCACGGAGCTTGTTTGCTTCAATCCGGGGTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGc                                                                                      >  2:441361/1‑140 (MQ=255)                                             gCATACCACGGAGCTTGTTTGCTTCAATCCGGGGTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGcc                                                                                     <  2:1115385/140‑1 (MQ=255)                                               aTACCACGGAGCTTGTTTGCTTCAATCCGGGGTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCAt                                                                                   <  1:66624/140‑1 (MQ=255)                                                tACCACGGAGCTTGTTTGCTTCAATCCGGGGTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATc                                                                                  <  1:1150130/140‑1 (MQ=255)                                                tACCACGGAGCTTGTTTGCTTCAATCCGGGGTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATc                                                                                  <  1:813912/140‑1 (MQ=255)                                                tACCACGGAGCTTGTTTGCTTCAATCCGGGGTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATc                                                                                  <  2:958862/140‑1 (MQ=255)                                                 aCCACGGAGCTTGTTTGCTTCAATCC‑GGGTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCag                                                                                >  1:1296424/1‑140 (MQ=255)                                                  ccACGGAGCTTGTTTGCTTCAATCCGGGGTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCag                                                                                >  1:1116734/1‑140 (MQ=255)                                                     cGGAGCTTGTTTGCTTCAATCCGGGGTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATc                                                                                  <  2:61411/135‑1 (MQ=255)                                                     cGGAGCTTGTTTGCTTCAATCCGGGGTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATc                                                                                  >  1:61411/1‑135 (MQ=255)                                                         gCTTGTTTGCTTCAATCC‑GGGTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATc                                                                        <  2:1296424/140‑1 (MQ=255)                                                          cTTGTTTGCTTCAATCCGGGGTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATc                                                                        >  2:946633/1‑140 (MQ=255)                                                          cTTGTTTGCTTCAATCCGGGGAGACTCAAGAACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATc                                                                        >  2:974868/1‑140 (MQ=255)                                                              tttGCTTCAATCCGGGGTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGc                                                                    <  1:260691/140‑1 (MQ=255)                                                                    tCAATCCGGGGTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTcaca                                                              >  1:90384/1‑140 (MQ=255)                                                                        tCCGGGGTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTa                                                          <  2:381717/140‑1 (MQ=255)                                                                        tCCGGGGTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTa                                                          <  2:923988/140‑1 (MQ=255)                                                                        tCCGGGGTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTa                                                          <  2:950380/140‑1 (MQ=255)                                                                         ccGGGGTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTAc                                                         <  1:441361/140‑1 (MQ=255)                                                                         ccGGGGTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTAc                                                         >  1:814573/1‑140 (MQ=255)                                                                             ggTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGc                                                     >  2:123273/1‑140 (MQ=255)                                                                             ggTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGc                                                     >  1:683028/1‑140 (MQ=255)                                                                              gTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGCt                                                    <  2:727477/140‑1 (MQ=255)                                                                                 aCTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGCTTAg                                                 <  2:242109/140‑1 (MQ=255)                                                                                  cTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGCTTAgg                                                <  1:242513/140‑1 (MQ=255)                                                                                  cTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGCTTAgg                                                >  2:932534/1‑140 (MQ=255)                                                                                  cTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGCTTAgg                                                >  1:1008126/1‑140 (MQ=255)                                                                                       gTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGCTTAGGATTAc                                           >  2:1084142/1‑140 (MQ=255)                                                                                            tCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGCTTAGGATTACGAAGc                                      >  1:998335/1‑140 (MQ=255)                                                                                             cAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTTGCTTAGGATTACGAAGcc                                     >  1:148334/1‑140 (MQ=255)                                                                                             cAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGCTTAGGATTACGAAGcc                                     >  1:1184156/1‑140 (MQ=255)                                                                                              aaCCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGCTTAGGATTACGAAGCCg                                    <  2:662548/140‑1 (MQ=255)                                                                                               aCCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGCTTAGGATTACGAAGCCGt                                   >  2:290898/1‑140 (MQ=255)                                                                                                ccAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGCTTAGGATTACGAAGCCGtt                                  <  2:202363/140‑1 (MQ=255)                                                                                                ccAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGCTTAGGATTACGAAGCCGtt                                  <  1:123273/140‑1 (MQ=255)                                                                                                   gCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGCTTAGGATTACGAAGCCGTTCtt                               >  1:591295/1‑140 (MQ=255)                                                                                                      tcttTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGCTTAGGATTACGAAGCCGTTCTTTCa                            <  2:998335/140‑1 (MQ=255)                                                                                                      tcttTTTCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGCTTAGGATTACGAAGCCGTTCTTTCa                            <  1:384678/140‑1 (MQ=255)                                                                                                        tttttCAACTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGCTTAGGATTACGAAGCCGTTCTTTCACt                          <  2:1194893/140‑1 (MQ=255)                                                                                                              aaCTGTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGCTTAGGATTACGAAGCCGTTCTTTCACTATCTcc                    >  1:1137567/1‑140 (MQ=255)                                                                                                                  gTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGCTTAGGATTACGAAGCCGTTCTTTCACTATCTCCACaa                >  2:1013516/1‑140 (MQ=255)                                                                                                                  gTTCACGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGCTTAGGATTACGAAGCCGTTCTTTCACTATCTCCACaa                <  1:379128/140‑1 (MQ=255)                                                                                                                       cGTACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGCTTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCa           <  1:974868/140‑1 (MQ=255)                                                                                                                         tACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGCTTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGc         <  2:707311/140‑1 (MQ=255)                                                                                                                         tACCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGCTTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGc         <  2:917589/140‑1 (MQ=255)                                                                                                                          aCCGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGCTTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCa        >  1:800355/1‑140 (MQ=255)                                                                                                                           ccGTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTTGCTTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCAt       <  2:148334/140‑1 (MQ=255)                                                                                                                             gTCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGCTTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCt     >  2:175737/1‑140 (MQ=255)                                                                                                                              tCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGCTTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCtt    <  2:1080833/140‑1 (MQ=255)                                                                                                                              tCGAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGCTTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCtt    <  2:427168/140‑1 (MQ=255)                                                                                                                                gAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGCTTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTtca  <  2:1137567/140‑1 (MQ=255)                                                                                                                                gAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGCTTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTtca  <  1:932534/140‑1 (MQ=255)                                                                                                                                gAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGCTTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTtca  <  1:377670/140‑1 (MQ=255)                                                                                                                                gAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGCTTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTtca  <  1:242397/140‑1 (MQ=255)                                                                                                                                gAGCCCCGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGCTTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTtca  <  1:1013516/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                      AAGGCGATAGCCTGAAGACCGGAGCTTAATCTTGTAACAATCAGGCATACCACGGAGCTTGTTTGCTTCAATCCGGGGTGACTCAAGTACTTCAACCAGCTTCTTTTTCAACTGTTCACGTACCGTCGAGCCCAGCTTTCGCCATTCCTTTAGTGCCCGCTCGTCAAAATCCAGAAAATACGCCATCAGAGTTCATCCAGCGTCACACGTACTGGCTTAGGATTACGAAGCCGTTCTTTCACTATCTCCACAAGTTCAGCATCTTCA  >  NC_000913/1645448‑1645714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑