BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,059,907	G→A	noncoding (57/76 nt)	asnU →	tRNA‑Asn

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,059,907	0	G	A	97.3%	374.0 / 0.1	110	noncoding (57/76 nt)	asnU	tRNA‑Asn
Reads supporting (aligned to +/- strand): ref base G (1/2); new base A (52/55); total (53/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

Only 100 of 111 total aligned reads displayed.

GAAACGGCAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTA  >  NC_000913/2059771‑2060042

gAAACGGCAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGt                                                                                                                                      <  2:1942165/140‑1 (MQ=255)

  aaCGGCAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCGGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTcc                                                                                                                                    >  1:1033744/1‑140 (MQ=255)

    cGGCAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAg                                                                                                                                  >  2:925451/1‑140 (MQ=255)

       cAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTTGCCGGTAAGGTGTGCCTCAAAAACGGTTTCTTTGTAGGTTAGGCGGGAGAACGGGGGACTTTTAAATCGGATTTTACTgggtcgggtctggctt                                                                                                                               >  2:604075/1‑134 (MQ=255)

       cAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCa                                                                                                                               >  2:1685138/1‑140 (MQ=255)

       cAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCa                                                                                                                               >  1:75257/1‑140 (MQ=255)

             gTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCTGTctcttata                                                                                                                         <  1:1897146/140‑8 (MQ=255)

                    aaTTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCGGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAAtt                                                                                                                  <  2:1033744/140‑1 (MQ=255)

                    aaTTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAAtt                                                                                                                  <  2:639519/140‑1 (MQ=255)

                      tttAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAg                                                                                                                                  <  1:319407/122‑1 (MQ=255)

                        tAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTg                                                                                                              >  1:334176/1‑140 (MQ=255)

                        tAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTg                                                                                                              >  1:1584999/1‑140 (MQ=255)

                        tAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTg                                                                                                              >  1:1945067/1‑140 (MQ=255)

                             aTCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAg                                                                                                         <  2:796738/140‑1 (MQ=255)

                             aTCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAg                                                                                                         <  1:820619/140‑1 (MQ=255)

                             aTCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAg                                                                                                         <  2:859772/140‑1 (MQ=255)

                               cGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGcc                                                                                                       <  2:328987/140‑1 (MQ=255)

                               cGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGcc                                                                                                       <  1:186512/140‑1 (MQ=255)

                                gggTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGccc                                                                                                      <  2:629368/140‑1 (MQ=255)

                                gggTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGccc                                                                                                      >  1:1322856/1‑140 (MQ=255)

                                     tGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCttt                                                                                                 >  2:1322913/1‑140 (MQ=255)

                                           aaGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGc                                                                                           <  2:1840404/140‑1 (MQ=255)

                                             gATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCgg                                                                                         >  2:10203/1‑140 (MQ=255)

                                                 tttCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGAtt                                                                                     <  2:834970/140‑1 (MQ=255)

                                                     gCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTg                                                                                 <  2:75257/140‑1 (MQ=255)

                                                     gCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTg                                                                                 <  2:1322856/140‑1 (MQ=255)

                                                     gCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTg                                                                                 <  1:1322913/140‑1 (MQ=255)

                                                     gCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTg                                                                                 <  1:1497000/140‑1 (MQ=255)

                                                      ccGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGc                                                                                <  1:792671/140‑1 (MQ=255)

                                                         ttAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCtat                                                                             >  1:923117/1‑140 (MQ=255)

                                                         ttAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCtat                                                                             <  2:1162348/140‑1 (MQ=255)

                                                              aTGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTg                                                                        <  2:802961/140‑1 (MQ=255)

                                                                gtgCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGAt                                                                      <  1:925451/140‑1 (MQ=255)

                                                                 tgCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATa                                                                     >  1:393415/1‑140 (MQ=255)

                                                                   ccTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGAtttt                                                                                   <  1:943080/124‑1 (MQ=255)

                                                                   ccTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAAt                                                                   >  1:19358/1‑140 (MQ=255)

                                                                   ccTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAAt                                                                   >  2:106622/1‑140 (MQ=255)

                                                                   ccTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAAt                                                                   >  1:1350722/1‑140 (MQ=255)

                                                                   ccTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAAt                                                                   >  2:516327/1‑140 (MQ=255)

                                                                      caacaaCGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTg                                                                                 >  1:1567873/1‑123 (MQ=255)

                                                                      caacaaCGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTg                                                                                 <  2:1567873/123‑1 (MQ=255)

                                                                         caacGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAAttt                                                             <  2:923117/140‑1 (MQ=255)

                                                                          cacGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTc                                                            <  2:779981/139‑1 (MQ=255)

                                                                          aacGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTc                                                            <  1:516327/140‑1 (MQ=255)

                                                                          aacGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTc                                                            <  2:393415/140‑1 (MQ=255)

                                                                             gATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCctc                                                         >  1:1456293/1‑140 (MQ=255)

                                                                              aTTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCctct                                                        >  2:795687/1‑140 (MQ=255)

                                                                               ttCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAAt                                                               >  2:631606/1‑132 (MQ=255)

                                                                               ttCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAAt                                                               <  1:631606/132‑1 (MQ=255)

                                                                                   tctGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACt                                                   >  1:1381813/1‑140 (MQ=255)

                                                                                    ctGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTg                                                  >  2:659101/1‑140 (MQ=255)

                                                                                    ctGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTg                                                  <  1:485132/140‑1 (MQ=255)

                                                                                     tGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGc                                                 <  2:1515851/140‑1 (MQ=255)

                                                                                     tGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGc                                                 <  2:19358/140‑1 (MQ=255)

                                                                                      gTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCt                                                >  2:1095982/1‑140 (MQ=255)

                                                                                       tAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCtt                                               >  2:418386/1‑140 (MQ=255)

                                                                                           tCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCa                                           <  2:1584999/140‑1 (MQ=255)

                                                                                            cAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCa                                           <  2:45089/139‑1 (MQ=255)

                                                                                            cAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCa                                           >  1:45089/1‑139 (MQ=255)

                                                                                            cAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCAt                                          >  2:782879/1‑140 (MQ=255)

                                                                                            cAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCAt                                          >  1:39259/1‑140 (MQ=255)

                                                                                            cAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCAt                                          >  2:1774352/1‑140 (MQ=255)

                                                                                               tCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCAc                                       >  1:1531598/1‑140 (MQ=255)

                                                                                               tCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCAc                                       >  2:351771/1‑140 (MQ=255)

                                                                                               tCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCAc                                       >  2:905262/1‑140 (MQ=255)

                                                                                               tCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCAc                                       <  1:418386/140‑1 (MQ=255)

                                                                                               tCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCAc                                       >  1:192496/1‑140 (MQ=255)

                                                                                               tCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCAc                                       >  2:865631/1‑140 (MQ=255)

                                                                                               tCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTT‑ACTGCt                                                <  1:340539/130‑1 (MQ=255)

                                                                                               tCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTT‑ACTGCt                                                >  2:340539/1‑130 (MQ=255)

                                                                                                cGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCAcc                                      <  2:1350722/140‑1 (MQ=255)

                                                                                                 ggTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCt                                     >  2:1920085/1‑140 (MQ=255)

                                                                                                   tAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGc                                   <  1:952662/140‑1 (MQ=255)

                                                                                                    aGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGcc                                  <  1:1095982/140‑1 (MQ=255)

                                                                                                    aGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGcc                                  <  1:1920085/140‑1 (MQ=255)

                                                                                                         ggcggACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTg                             <  2:39259/140‑1 (MQ=255)

                                                                                                         ggcggACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTg                             <  2:1945067/140‑1 (MQ=255)

                                                                                                           cggACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGat                           <  2:1456293/140‑1 (MQ=255)

                                                                                                           cggACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGat                           <  1:1774352/140‑1 (MQ=255)

                                                                                                             gACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCa                                        >  2:691178/1‑125 (MQ=255)

                                                                                                             gACTGTTAATCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGatat                         <  2:35521/140‑1 (MQ=255)

                                                                                                                     aTCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACt                 >  2:262882/1‑140 (MQ=255)

                                                                                                                     aTCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACt                 <  1:1262823/140‑1 (MQ=255)

                                                                                                                     aTCCGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACt                 >  2:289422/1‑140 (MQ=255)

                                                                                                                       ccGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGa               <  2:192496/140‑1 (MQ=255)

                                                                                                                       ccGTATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTACTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGa               <  1:1193424/140‑1 (MQ=255)

                                                                                                                          tATGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAg            >  1:634559/1‑140 (MQ=255)

                                                                                                                            tGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGa               <  2:1824872/135‑1 (MQ=255)

                                                                                                                            tGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGa               >  1:1824872/1‑135 (MQ=255)

                                                                                                                            tGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAg            <  2:191506/138‑1 (MQ=255)

                                                                                                                            tGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTc          >  1:666363/1‑140 (MQ=255)

                                                                                                                            tGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTc          <  1:905262/140‑1 (MQ=255)

                                                                                                                            tGTCACTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCAGCTTGATATCCTCAACTGACAGTc          <  1:795687/140‑1 (MQ=255)

                                                                                                                                aCTGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGc      >  1:1736127/1‑140 (MQ=255)

                                                                                                                                  tGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTc          <  2:1777745/134‑1 (MQ=255)

                                                                                                                                  tGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTc          >  1:1777745/1‑134 (MQ=255)

                                                                                                                                  tGGTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCAt    >  1:247294/1‑140 (MQ=255)

                                                                                                                                    gTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGa               <  1:307140/127‑1 (MQ=255)

                                                                                                                                    gTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGa               >  2:307140/1‑127 (MQ=255)

                                                                                                                                    gTTCAAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTa  >  2:1241049/1‑140 (MQ=255)

GAAACGGCAAACAGTTGGGGAATTTAAAAATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTA  >  NC_000913/2059771‑2060042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`