Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	847,968	T→G	T13P (ACC→CCC)	glnH ←	L‑glutamine ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	847,968	0	T	G	100.0%	360.1 / NA	91	T13P (ACC→CCC)	glnH	L‑glutamine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (44/47);  total (44/47)

TGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACGGGACGATCCCCTGCGTGACATTCACATATATGAAAAAATCGTGCCAGTTTTGCAACTCCTTGATTTTTCGAGATGC  >  NC_000913/847836‑848104                                                                                                                                     |                                                                                                                                         tGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCa                                                                                                                                   <  2:791551/140‑1 (MQ=255)   gCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGc                                                                                                                                 >  1:418006/1‑140 (MQ=255)   gCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGc                                                                                                                                 >  1:523287/1‑140 (MQ=255)   gCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGc                                                                                                                                 >  2:1173259/1‑140 (MQ=255)   gCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGc                                                                                                                                 >  1:1266234/1‑140 (MQ=255)   gCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGc                                                                                                                                 >  2:1826070/1‑140 (MQ=255)         cacaGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTAACCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGa                                                                                                                           <  1:1874436/140‑1 (MQ=255)          acaGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGaa                                                                                                                          <  2:1156741/140‑1 (MQ=255)          acaGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGaa                                                                                                                          <  1:1040567/140‑1 (MQ=255)            aGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAAc                                                                                                                        <  1:1346858/140‑1 (MQ=255)            aGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAAc                                                                                                                        <  2:418006/140‑1 (MQ=255)            aGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAAc                                                                                                                        <  1:1104231/140‑1 (MQ=255)              aTCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACtt                                                                                                                      >  2:1349797/1‑140 (MQ=255)              aTCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACtt                                                                                                                      <  1:1349797/140‑1 (MQ=255)              aTCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACtt                                                                                                                      <  2:530389/140‑1 (MQ=255)               tCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACttt                                                                                                                     <  1:1173259/140‑1 (MQ=255)                     gcAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATAc                                                                                                               <  2:1893913/139‑1 (MQ=255)                      cAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACa                                                                                                              <  1:236155/140‑1 (MQ=255)                      cAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACa                                                                                                              <  1:518311/140‑1 (MQ=255)                      cAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACa                                                                                                              >  2:1857257/1‑140 (MQ=255)                        aaccccACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGa                                                                                                            >  2:1485759/4‑140 (MQ=255)                        aaGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGaa                                                                                                                          <  2:375289/126‑1 (MQ=255)                        aaGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGaa                                                                                                                          >  1:375289/1‑126 (MQ=255)                        aaGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGa                                                                                                            <  1:841919/140‑1 (MQ=255)                        aaGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGa                                                                                                            >  2:932376/1‑140 (MQ=255)                               caTATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATa                                                                                                     <  1:1316691/140‑1 (MQ=255)                                atatTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAg                                                                                                    >  1:45080/1‑140 (MQ=255)                                     tATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCAttt                                                                                               >  1:594332/1‑140 (MQ=255)                                      aTCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTc                                                                                              >  2:1529226/1‑140 (MQ=255)                                       tCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTcc                                                                                             <  1:969795/140‑1 (MQ=255)                                        cGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCt                                                                                            <  1:1857257/140‑1 (MQ=255)                                          cccTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGa                                                                                                            <  1:327430/122‑1 (MQ=255)                                          cccTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGa                                                                                                            >  1:489710/1‑122 (MQ=255)                                          cccTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGa                                                                                                            <  2:489710/122‑1 (MQ=255)                                          cccTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGa                                                                                                            >  2:327430/1‑122 (MQ=255)                                          cccTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCttt                                                                                          >  2:1086174/1‑140 (MQ=255)                                          cccTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCttt                                                                                          >  1:756395/1‑140 (MQ=255)                                          cccTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCttt                                                                                          >  2:654566/1‑140 (MQ=255)                                          cccTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCttt                                                                                          >  2:438527/1‑140 (MQ=255)                                          cccTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCttt                                                                                          >  2:70074/1‑140 (MQ=255)                                          cccTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCttt                                                                                          >  2:517291/1‑140 (MQ=255)                                            cTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCttttt                                                                                        >  2:541543/1‑140 (MQ=255)                                             tGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCtttttt                                                                                       >  2:342586/1‑140 (MQ=255)                                             tGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCtttttt                                                                                       <  1:654566/140‑1 (MQ=255)                                             tGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCtttttt                                                                                       <  1:227022/140‑1 (MQ=255)                                             tGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCtttttt                                                                                       <  2:527572/140‑1 (MQ=255)                                               tttAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCTTTTTTCt                                                                                     <  2:364857/140‑1 (MQ=255)                                                    aTTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCa                                                                                <  2:523287/140‑1 (MQ=255)                                                      tCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACg                                                                              <  2:756395/140‑1 (MQ=255)                                                      tCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACg                                                                              <  2:1639882/140‑1 (MQ=255)                                                        aaaCGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACggg                                                                            <  1:517291/140‑1 (MQ=255)                                                         aaCGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACGGGa                                                                           >  1:1446679/1‑140 (MQ=255)                                                         aaCGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACGGGa                                                                           >  1:1299253/1‑140 (MQ=255)                                                         aaCGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACGGGa                                                                           >  2:1485025/1‑140 (MQ=255)                                                                cGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACGGGACGATccc                                                                    >  1:1485319/1‑140 (MQ=255)                                                                cGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACGGGACGATccc                                                                    <  1:430415/140‑1 (MQ=255)                                                                  aaGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACGGGACGATCCCCt                                                                  >  1:298197/1‑140 (MQ=255)                                                                      cGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACGGGACGATCCCCTGCGt                                                              <  2:1061453/140‑1 (MQ=255)                                                                       ggTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACGGGACGATCCCCTGCGTg                                                             <  1:1529226/140‑1 (MQ=255)                                                                        gTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACg                                                                              <  1:1169093/122‑1 (MQ=255)                                                                        gTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACg                                                                              >  2:1169093/1‑122 (MQ=255)                                                                        gTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACGGGACGATCCCCTGCGTGa                                                            >  1:513251/1‑140 (MQ=255)                                                                        gTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACGGGACGATCCCCTGCGTGa                                                            <  2:45080/140‑1 (MQ=255)                                                                        gTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACGGGACGATCCCCTGCGTGa                                                            >  1:917472/1‑140 (MQ=255)                                                                           tCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACGGGACGATCCCCTGCGTGACa                                                          >  1:1947836/1‑139 (MQ=255)                                                                           tCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACGGGACGATCCCCTGCGTGACa                                                          <  2:1947836/139‑1 (MQ=255)                                                                            ccGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACGGGACGATCCCCTGCGTGACAtt                                                        <  2:1446679/140‑1 (MQ=255)                                                                              gTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACGGGACGATCCCCTGCGTGACATTca                                                      <  1:1826070/140‑1 (MQ=255)                                                                              gTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACGGGACGATCCCCTGCGTGACATTca                                                      <  2:917472/140‑1 (MQ=255)                                                                              gTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACGGGACGATCCCCTGCGTGACATTca                                                      <  2:1266234/140‑1 (MQ=255)                                                                              gTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACGGGACGATCCCCTGCGTGACATTca                                                      <  2:513251/140‑1 (MQ=255)                                                                               tCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACGGGACGATCCCCTGCGTGACATTcac                                                     >  1:736988/1‑140 (MQ=255)                                                                               tCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACGGGACGATCCCCTGCGTGACATTcac                                                     >  2:1324579/1‑140 (MQ=255)                                                                                cgcgACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACGGGACGATCCCCTGCGTGACATTcaca                                                    >  1:1457249/1‑140 (MQ=255)                                                                                cgcgACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACGGGACGATCCCCTGCGTGACATTcaca                                                    <  1:583825/140‑1 (MQ=255)                                                                                  cgACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACGGGACGATCCCCTGCGTGACATTCACata                                                  <  2:298197/140‑1 (MQ=255)                                                                                     cAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACGGGACGATCCCCTGCGTGACATTCACatatat                                               >  2:389360/1‑140 (MQ=255)                                                                                     cAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACGGGACGATCCCCTGCGTGACATTCACatatat                                               >  2:48782/1‑140 (MQ=255)                                                                                                     cgcgGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACGGGACGATCCCCTGCGTGACATTCACATATATGAAAAAATCGTGCCAg                               <  1:389360/140‑1 (MQ=255)                                                                                                     cgcgGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACGGGACGATCCCCTGCGTGACATTCACATATATGAAAAAATCGTGCCAg                               <  1:70074/140‑1 (MQ=255)                                                                                                       cgGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACGGGACGATCCCCTGCGTGACATTCACATATATGAAAAAATCGTGc                                  >  1:1516892/1‑135 (MQ=255)                                                                                                       cgGCATGAGAAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACGGGACGATCCCCTGCGTGACATTCACATATATGAAAAAATCGTGc                                  <  2:1516892/135‑1 (MQ=255)                                                                                                             gagaAGAAACCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACGGGACGATCCCCTGCGTGACATTCACATATATGAAAAAATCGTGCCAGTTTTGCaa                       <  1:662811/140‑1 (MQ=255)                                                                                                                    aaCCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACGGGACGATCCCCTGCGTGACATTCACATATATGAAAAAATCGTGCCAGTTTTGCAACTCCTTg                >  1:244604/1‑140 (MQ=255)                                                                                                                     aCCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACGGGACGATCCCCTGCGTGACATTCACATATATGAAAAAATCGTGCCAGTTTTGCAACTCCTTGa               >  2:528722/1‑140 (MQ=255)                                                                                                                     aCCGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACGGGACGATCCCCTGCGTGACATTCACATATATGAAAAAATCGTGCCAGTTTTGCAACTCCTTGa               >  2:903966/1‑140 (MQ=255)                                                                                                                       cGCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACGGGACGATCCCCTGCGTGACATTCACATATATGAAAAAATCGTGCCAGTTTTGCAACTCCTTGAtt             >  2:660495/1‑140 (MQ=255)                                                                                                                        gCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACGGGACGATCCCCTGCGTGACATTCACATATATGAAAAAATCGTGCCAGTTTTGCAACTCCTTGAttt            >  2:1421965/1‑140 (MQ=255)                                                                                                                        gCAAAAGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACGGGACGATCCCCTGCGTGACATTCACATATATGAAAAAATCGTGCCAGTTTTGCAACTCCTTGAttt            >  2:350725/1‑140 (MQ=255)                                                                                                                          aaaaGCCAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACGGGACGATCCCCTGCGTGACATTCACATATATGAAAAAATCGTGCCAGTTTTGCAACTCCTTGAttttt          >  2:310003/1‑140 (MQ=255)                                                                                                                               ccAGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACGGGACGATCCCCTGCGTGACATTCACATATATGAAAAAATCGTGCCAGTTTTGCAACTCCTTGATTTTTCgaga     <  1:343255/140‑1 (MQ=255)                                                                                                                                 aGGGGCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACGGGACGATCCCCTGCGTGACATTCACATATATGAAAAAATCGTGCCAGTTTTGCAACTCCTTGATTTTTCGAGATg   <  1:1043880/140‑1 (MQ=255)                                                                                                                                  ggggCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACGGGACGATCCCCTGCGTGACATTCACATATATGAAAAAATCGTGCCAGTTTTGCAACTCCTTGATTTTTCGAGATGc  <  1:528722/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                         TGGCAGCCCACAGATCAACGTCAAAGCCCACATATTTATCGCCCTGTTTAAATTCAAACGGAACGAAGGCGGTATCCGTCGCGACAACTAATTTTTTATCCGCGGCATGAGAAGAAACCGCAAAAGCCAGGGTCAGTGCAGCCAGTGAAACTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACGGGACGATCCCCTGCGTGACATTCACATATATGAAAAAATCGTGCCAGTTTTGCAACTCCTTGATTTTTCGAGATGC  >  NC_000913/847836‑848104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑