Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,350,8530AC26.5% 63.5 / 10.1 34S725A (TCA→GCA) arcBsensory histidine kinase ArcB
Reads supporting (aligned to +/- strand):  ref base A (24/1);  new base C (0/9);  total (24/10)
Fisher's exact test for biased strand distribution p-value = 1.91e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGT  >  NC_000913/3350723‑3350992
                                                                                                                                  |                                                                                                                                           
accccTTCTCCGTCGTGCCCCCCCTCTTCTTTCATCTCTTCAACCCATCCCCCGCCTTTATCTCCCCAGGCGGGAAGGTCAGGAGACTGATTTTGCTGCCCCAGCTGTTGCAGATGGCGAACCCCCCCGGCCCCcgccgc                                                                                                                                    <  2:1002726/136‑1 (MQ=255)
    cTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCccgcctgtctc                                                                                                                                >  1:719233/1‑134 (MQ=255)
       cTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTa                                                                                                                             >  1:27073/1‑140 (MQ=255)
          cgtcgtGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAAtt                                                                                                                          >  2:1529961/1‑140 (MQ=255)
               tGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATg                                                                                                                     >  2:881560/1‑140 (MQ=255)
               tGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATg                                                                                                                     >  2:58007/1‑140 (MQ=255)
               tGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATg                                                                                                                     >  1:622834/1‑140 (MQ=255)
               tGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATg                                                                                                                     >  1:275374/1‑140 (MQ=255)
                gccccccctctTCTTTCATCTCTTCAACCCATCCCCCGACTTTATCTCCCCAGGCCGGAAGGTCAGGAGACTAATTTTGCTGCCCCAGTTGTTGCAGATGGCGTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGt                                                                                                                    <  2:275374/133‑1 (MQ=255)
                gACCCCCCTCTTCTTTCATCTCTTCAACCCATTCCCCGACTTTATCTCCCCAGGCGGGAAGGTCAGGAGACTAATTTTGCTGCCCCAGTTTTTGCAGATGGCGTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGt                                                                                                                    <  2:965020/140‑1 (MQ=255)
                  cccccctctTCTTTCATCTCTTCAACCCATCCCCCGACTTTATCTCCCCAGGCCGGAGGGTCAGGAGACTAATTTTGCTGCCCCAGTTTTTGCAGATGGCGTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGtcc                                                                                                                  <  2:1282139/135‑1 (MQ=255)
                                  tctcTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCt                                                                                                  >  2:872202/1‑140 (MQ=255)
                                             cATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTg                                                                                       >  1:523715/1‑140 (MQ=255)
                                                     gACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAA‑TTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAg                                                                              >  1:200184/1‑140 (MQ=255)
                                                      aCGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAg                                                                              >  1:357146/1‑140 (MQ=255)
                                                          tATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTc                                                                          >  1:510356/1‑140 (MQ=255)
                                                                 ccAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCa                                                                   >  2:1280528/1‑140 (MQ=255)
                                                                  cAGGCCGGAGGGCCGGGGGCCTAATTTTGCTCACCCAGCTGTGAAAGGGGGCCAACCCCCCCGTCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAg                                                                  <  2:622834/140‑1 (MQ=255)
                                                                     gcgggAAGGTCAGGAGACTGATTTTGCTGCCCCAGCTGTTGCAGAGGGCGTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCAc                                                               <  2:632606/137‑1 (MQ=255)
                                                                     gCCGGAGGGTAAGGAGAAAGATTTTGCTGCCCCAGCTGTTAAAGGTGCCGAACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCAc                                                               <  2:183969/140‑1 (MQ=255)
                                                                     gCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCAc                                                               >  1:132981/1‑140 (MQ=255)
                                                                      cgggAAGGTCAGGAGACTAATTTTGCTGCCCCAGTTGTTGCAGATGGCGAACCCCCCCTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACg                                                              <  2:1455733/138‑1 (MQ=255)
                                                                         gAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTg                                                           >  1:1455733/1‑140 (MQ=255)
                                                                         gAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTg                                                           >  2:845246/1‑140 (MQ=255)
                                                                          aaGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGa                                                          >  2:1386254/1‑140 (MQ=255)
                                                                               cAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAg                                                     >  1:1120519/1‑140 (MQ=255)
                                                                                aGGAAAGTGATTTTGTTCCCCCTGTTGTTGAGGGTGTCGTACCCCCACCGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGc                                                    <  2:357146/140‑1 (MQ=255)
                                                                                 ggAGACTGAATTTGCTGCCCCAGTTGTTGCAGATGGCGTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGcc                                                   <  1:1529961/140‑1 (MQ=255)
                                                                                           tttGCTGCCCCAGTTGTTGCAGATGGCGTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCatcatc                                         <  1:1024676/140‑1 (MQ=255)
                                                                                           tttGCTGCCCCAGTTGTTGCAGAGGGCGTACCCCCCCGGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCatcatc                                         <  1:671750/140‑1 (MQ=255)
                                                                                                   cccAGCTTTTGCAGAGGGCGTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCaaa                                 <  1:58007/140‑1 (MQ=255)
                                                                                                       gCTGTTGCAGATGGCGTAACCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCaaa                                 <  2:250490/136‑1 (MQ=255)
                                                                                                       gCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCaaa                                 >  1:250490/1‑136 (MQ=255)
                                                                                                         ttttGCAGTGGGCCTACCCCCCCTCCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGc                           <  2:523715/138‑1 (MQ=255)
                                                                                                          gTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCt                          <  1:845246/140‑1 (MQ=255)
                                                                                                            tGCAGATGGCGTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTaa                        <  1:563623/140‑1 (MQ=255)
                                                                                                                     cGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGt               >  1:646974/1‑140 (MQ=255)
                                                                                                                       tAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGgaga             >  2:77542/1‑140 (MQ=255)
                                                                                                                       tAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGa             >  1:1026844/1‑140 (MQ=255)
                                                                                                                             cccTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATGGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCt       <  2:232415/138‑1 (MQ=255)
                                                                                                                              aCTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCtt      >  1:554748/1‑140 (MQ=255)
                                                                                                                               cTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTc     >  2:1479236/1‑140 (MQ=255)
                                                                                                                                tGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCg    <  2:1120519/140‑1 (MQ=255)
                                                                                                                                  aCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGt  >  1:1236533/1‑140 (MQ=255)
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AGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGT  >  NC_000913/3350723‑3350992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑