| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,032,731 | 0 | T | G | 23.1% | 105.9 / 10.7 | 52 | G80G (GGT→GGG) | yigZ | IMPACT family member YigZ |
| Reads supporting (aligned to +/- strand): ref base T (12/28); new base G (12/0); total (24/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.31e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGGCGAAAGCGTTTGTTGAATCGGTGCGGGCAGAACACCCCGATGCCCGGCACCATTGCGTGGCGTGGGTCGCGGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGTGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTGCGCC > NC_000913/4032592‑4032870 | cggcgAAAGCGTTTGTTGAATCGGTGCGGGCAGAACACCCCGATGCCCGGCACCATTGCGTGGCGTGGGTCGCGGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGt < 1:885884/140‑1 (MQ=255)cggcgAAAGCGTTTGTTGAATCGGTGCGGGCAGAACACCCCGATGCCCGGCACCATTGCGTGGCGTGGGTCGCGGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGt < 2:1465995/140‑1 (MQ=255)cggcgAAAGCGTTTGTTGAATCGGTGCGGGCAGAACACCCCGATGCCCGGCACCATTGCGTGGCGTGGGTCGCGGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGt < 2:239794/140‑1 (MQ=255) gAAAGCGTTTGTTGAATCGGTGCGGGCAGAACACCCCGATGCCCGGCACCATTGCGTGGCGTGGGTCGCGGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAAc < 2:141828/140‑1 (MQ=255) tgttgAATCGGTGCGGGCAGAACACCCCGATGCCCGGCACCATTGCGTGGCGTGGGTCGCGGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCg < 1:383677/140‑1 (MQ=255) tgttgAATCGGTGCGGGCAGAACACCCCGATGCCCGGCACCATTGCGTGGCGTGGGTCGCGGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCg < 2:286450/140‑1 (MQ=255) tgttgAATCGGTGCGGGCAGAACACCCCGATGCCCGGCACCATTGCGTGGCGTGGGTCGCGGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGGAAAACGATGCTcc > 2:20863/1‑139 (MQ=255) gttgAATCGGTGCGGGCAGAACACCCCGATGCCCGGCACCATTGCGTGGCGTGGGTCGCGGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGc < 1:120696/140‑1 (MQ=255) gttgAATCGGTGCGGGCAGAACACCCCGATGCCCGGCACCATTGCGTGGCGTGGGTCGCGGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGc < 1:132202/140‑1 (MQ=255) ttgAATCGGTGCGGGCAGAACACCCCGATGCCCGGCACCATTGCGTGGCGTGGGTCGCGGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGcc < 1:1532411/140‑1 (MQ=255) tgAATCGGTGCGGGCAGAACGCCCCGATGCCCGGCACCATTGCGTGGCGTGGGTCGCGGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGccc < 1:1112079/140‑1 (MQ=255) tgAATCGGTGCGGGCAGAACACCCCGATGCCCGGCACCATTGCGTGGCGTGGGTCGCGGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGccc < 1:1343798/140‑1 (MQ=255) cGGGCAGAACACCCCGATGCCCGGCACCATTGCGTGGCGTGGGTCGCGGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGCAAACCTGTGCGCGCCCCGGCAATggg > 2:1112079/1‑140 (MQ=255) cccGATGCCCGGCACCATTGCGTGGCGTGGGTCGCGGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCgg > 1:1043805/1‑140 (MQ=255) cccGATGCCCGGCACCATTGCGTGGCGTGGGTCGCGGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGGAAACCGATGCTCGCCCCGCTAATGGGCAGcggcggcgg > 2:936632/1‑140 (MQ=255) tGCCCGGCACCATTGCGTGGCGTGGGTCGCGGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGaaa > 1:630176/1‑140 (MQ=255) gCCCGGCACCATTGCGTGGCGTGGGTCGCGGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAAt < 1:373754/140‑1 (MQ=255) cGGCACCATTGCGTGGCGTGGGTCGCGGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTAc < 1:1202404/140‑1 (MQ=255) cGGCACCATTGCGTGGCGTGGGTCGCGGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTAc > 1:798983/1‑140 (MQ=255) ggCACCATTGCGTGGCGTGGGTCGCGGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGGAATTAcc > 1:1083695/1‑140 (MQ=255) ggCACCATTGCGTGGCGTGGGTCGCGGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGGAAACCCATGCTCGCCCCGCTAATGGGCAGCGGCGTCGGGGAAATTAcc > 1:626239/1‑140 (MQ=255) ggCACCATTGCGTGGCGTGGGTCGCGGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGGAAAACGATGCTCGCCCCGCTAATGGGCAGCGGGGGCGGGGGAAATAcc > 2:1202404/1‑140 (MQ=255) gCACCATTGCGTGGCGTGGGTCGCGGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCg < 2:935042/140‑1 (MQ=255) gCACCATTGCGTGGCGTGGGTCGCGGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCg < 2:630176/140‑1 (MQ=255) ccATTGCGTGGCGTGGGTCGCGGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCtg < 1:752620/140‑1 (MQ=255) ccATTGCGTGGCGTGGGTCGCGGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCtg < 1:20863/140‑1 (MQ=255) cATTGCGTGGCGTGGGTCGCGGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCtgt > 1:1232240/1‑140 (MQ=255) ttGCGTGGCGTGGGTCGCGGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGGAAACCGATGCTCGCCCCGCTAATGGGCAGCGGCGGCGGGGGAAATTCCGCTGTgg > 2:1184667/1‑140 (MQ=255) tGCGTGGCGTGGGTCGCGGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGGAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGTGGt > 1:405295/1‑140 (MQ=255) tggcgtggGTCGCGGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGGAAACCGATGCTCGCCCCGCTAATGGGCCGCGGCGGCGGGGGAAATTCCCCCGTGGGAggg > 2:1435155/1‑138 (MQ=255) cgtggGTCGCGGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGGAAACCGATGCTCGCCCCGCTAATGGGGAGCGGCGGCGGGGGAATTACCGCTGTGGGAGTgggc > 2:1162262/1‑140 (MQ=255) tggGTCGCGGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGGAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGGCGGGGGAAATACCGCTGTGGGAGTGCGcta > 2:504120/1‑140 (MQ=255) gggTCGCGGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGTGGTAGTGCGctac < 2:612040/140‑1 (MQ=255) cgGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGTGGTAGTGCGCTACTAcggc > 1:185388/1‑140 (MQ=255) cgGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGGAAAACGATGCTCGCCCAGCTAATGGGCAGCGGCGGCGGGGGAAATACCGCTGTGGTAGTGCGCTACTAcggc > 2:512591/1‑140 (MQ=255) gggTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGTGGTAGTGCGCTACTAcggcg < 2:1043805/140‑1 (MQ=255) gatgatTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGTGGTAGTGCGCTACTACGGCGGCATATTGCt < 1:1182724/140‑1 (MQ=255) gatgatTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGTGGTAGTGCGCTACTACGGCGGCATATTGCt < 1:278126/140‑1 (MQ=255) atgatTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGGAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGGCGGGGGAAATACCGCTGTGGGAGTGGGCTACTACGGCGGCGTATTGCtt > 2:1449302/1‑140 (MQ=255) tgatTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGGAGCGGCGGCGGGGGAAATACCGCTGTGGGAGGGCGCTACTACGGGGGGATATTTCTTg > 2:1380268/1‑140 (MQ=255) tgatTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGTGGTAGTGCGCTACTACGGCGGCATATTGCTTg < 1:1184667/140‑1 (MQ=255) gatTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGTGGTAGTGCGCTACTACGGCGGCATATTGCTTgg < 2:1155104/140‑1 (MQ=255) tcAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGTGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCg < 1:379214/140‑1 (MQ=255) tcAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGTGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCg < 2:405295/140‑1 (MQ=255) tcAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGTGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCg < 2:852093/140‑1 (MQ=255) aaCAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGTGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGt < 1:1449302/140‑1 (MQ=255) cTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCCGCTAATGGGCAGCGGCGGCGGGGGAAATACCGCTGTGGTAGTGCGCTACTACGGCGGCCTATTGCTTGGGACCgggggggt > 2:1111279/1‑138 (MQ=255) cTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGGAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGTGGGAGTGCGCTACTACGGCGGCCTATTGCTTTGGACCCGgggggt > 2:525310/1‑138 (MQ=255) gggTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGTGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAg < 2:185388/140‑1 (MQ=255) ggTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGTGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGt < 1:525310/140‑1 (MQ=255) ggTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCCATGCTCCCCCAGCTAAAGGGCAGCGGCGGCGGGGGAAATAACGCTGTGGTAGTGGGCTACTACCGCGGCATATTTCTTTGGACCCGGGGGGTAgg > 2:157865/1‑139 (MQ=255) ggTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCCAAGCTCCCCCCGCTAATTGGGAGCGGCGTCGGGGAAATTACCGCTGTGGTAGTGGGCTACTACGGCGGCATATTGCTTTGGCCCgggggggtggg > 2:1474262/1‑135 (MQ=255) tttCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGGAAACCCATGCTCGCCCCGCTAATGGGGAGCGGGGTCGGGGGAAATACCGCTGTGGGAGTGCGCTACTACGGCGGCATATTTGTTTGGACCgggggggttatgg > 2:1197475/1‑133 (MQ=255) cgacgGGGAGCCGGCGGGAACGGCAGGGAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGGAAATACCGCTGTGGTAGTGCGCTACTACGGCGGCATATTGCTTGGGACCGGTGGGGTAGTGAAAACGGATg > 2:657027/1‑140 (MQ=255) ggggAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGTGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATggcgg > 1:133693/1‑140 (MQ=255) gggAGCCGGCGGGAACGGCAGGGAAAACGAAGCTCGCCCCGCTAATGGGCAGCGGCGGCGGGGAAAATACCGCTGTGGTAGTGCGCTACTACGGCGGCATAATGCTTTGCACCGGTGGGGTAGTGAAAGCGTATgggggg > 2:488667/1‑139 (MQ=255) ggAGCCGGGGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGTGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATggcggcg > 2:1343119/1‑140 (MQ=255) gcggGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGTGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGaa > 1:36677/1‑140 (MQ=255) gcggGAACGGCAGGGAAACCGATGCTCGCCCCGCTAATGGGGAGCGGCGTCGGGGAAATTACCGCTGTGGGAGGGCGCTACTACGGCGGCATATTTCTTGGCACCGGGGGGGTAGGGGAAGCGTATGGCGGCGGCGGGaa > 2:550398/1‑140 (MQ=255) cGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGTGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGgcg < 1:1197475/140‑1 (MQ=255) gTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGTGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTgcgc < 2:1342703/140‑1 (MQ=255) tAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGTGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCCGCGGCGTGAATCAGGCGCTGCGcc < 2:1232240/140‑1 (MQ=255) | CGGCGAAAGCGTTTGTTGAATCGGTGCGGGCAGAACACCCCGATGCCCGGCACCATTGCGTGGCGTGGGTCGCGGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGTGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTGCGCC > NC_000913/4032592‑4032870 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |