Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	759,303	A→C	K200Q (AAA→CAA)	sucA →	subunit of E1(0) component of 2‑oxoglutarate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	759,303	0	A	C	100.0%	302.3 / NA	80	K200Q (AAA→CAA)	sucA	subunit of E1(0) component of 2‑oxoglutarate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (45/35);  total (45/35)

AGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCT‑GGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCGCC  >  NC_000913/759169‑759438                                                                                                                                        |                                                                                                                                        aGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCT‑GGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGcaaaaa                                                                                                                                    <  2:118695/140‑1 (MQ=255) aGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCT‑GGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGcaaaaa                                                                                                                                    <  2:120032/140‑1 (MQ=255)           aGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCT‑GGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTaa                                                                                                                          <  2:596797/140‑1 (MQ=255)                tCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCT‑GGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGaa                                                                                                                     <  2:714752/140‑1 (MQ=255)                       aaCCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCT‑GGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGAccg                                                                                                              <  1:50416/140‑1 (MQ=255)                              tGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCT‑GGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGa                                                                                                       >  1:1072555/1‑140 (MQ=255)                              tGCGGCCCGACTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCT‑GGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGa                                                                                                       <  2:1072555/140‑1 (MQ=255)                              ggCGGCCCGATTGGTGCCGAGTATATGCACATTGCCAGCACCGAAGAAAAACGCT‑GGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAACCGCTTCTTAAGCGAACTGGCCGCCGCTGa                                                                                                       <  2:866653/139‑1 (MQ=255)                               gCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCT‑GGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGaa                                                                                                      <  1:475356/140‑1 (MQ=255)                                cGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCT‑GGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAg                                                                                                     >  1:1007815/1‑140 (MQ=255)                                  gCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCT‑GGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGAc                                                                                                                <  1:1383753/127‑1 (MQ=255)                                  gCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCT‑GGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGAc                                                                                                                >  2:1383753/1‑127 (MQ=255)                                  gCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCT‑GGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGt                                                                                                   >  1:668411/1‑140 (MQ=255)                                  gCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCT‑GGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGt                                                                                                   >  1:812045/1‑140 (MQ=255)                                  gCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCT‑GGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGt                                                                                                   >  2:876064/1‑140 (MQ=255)                                   cccGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCT‑GGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTc                                                                                                  >  2:444507/1‑140 (MQ=255)                                   cccGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCT‑GGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTc                                                                                                  <  1:745951/140‑1 (MQ=255)                                      gATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCT‑GGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTg                                                                                               <  1:1196657/140‑1 (MQ=255)                                      gATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCT‑GGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTg                                                                                               <  1:1055402/140‑1 (MQ=255)                                                  gTATATGCACATTACCAGCACCGAAGAAAAACGCT‑GGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCg                                                                                   <  2:1153373/140‑1 (MQ=255)                                                     tatGCACATTACCAGCACCGAAGAAAAACGCT‑GGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGgcg                                                                                <  2:1199945/140‑1 (MQ=255)                                                        gCACATTACCAGCACCGAAGAAAAACGCT‑GGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGgcg                                                                                >  1:505662/1‑137 (MQ=255)                                                        gCACATTACCAGCACCGAAGAAAAACGCT‑GGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGgcg                                                                                <  2:505662/137‑1 (MQ=255)                                                             ttACCAGCACCGAAGAAAAACGCT‑GGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTATCTCGGCGCAAAATTc                                                                        >  2:619275/1‑140 (MQ=255)                                                             ttACCAGCACCGAAGAAAAACGCT‑GGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTc                                                                        >  1:638079/1‑140 (MQ=255)                                                             ttACCAGCACCGAAGAAAAACGCT‑GGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTc                                                                        >  1:682121/1‑140 (MQ=255)                                                             ttACCAGCACCGAAGAAAAACGCT‑GGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTc                                                                        >  2:1430721/1‑140 (MQ=255)                                                             ttACCAGCACCGAAGAAAAACGCT‑GGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTc                                                                        >  1:729571/1‑140 (MQ=255)                                                             ttACCAGCACCGAAGAAAAACGCT‑GGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTc                                                                        >  1:1146228/1‑140 (MQ=255)                                                             ttACCAGCACCGAAGAAAAACGCT‑GGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTc                                                                        >  2:317012/1‑140 (MQ=255)                                                             ttACCAGCACCGAAGAAAAACGCT‑GGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTc                                                                        >  2:543463/1‑140 (MQ=255)                                                             ttACCAGCACCGAAGAAAAACGCT‑GGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTc                                                                        >  2:1046307/1‑140 (MQ=255)                                                             ttACCAGCACCGAAGAAAAACACT‑GGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTc                                                                        >  2:982437/1‑140 (MQ=255)                                                               aCCAGCACCGAAGAAAAACGCT‑GGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTccc                                                                      >  2:442906/1‑140 (MQ=255)                                                                ccAGCACCGAAGAAAAACGCTGGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTccc                                                                      <  1:1465658/140‑1 (MQ=255)                                                                   gCACCGAAGAAAAACGCT‑GGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGgc                                                                  >  2:1299362/1‑140 (MQ=255)                                                                    cACCGAAGAAAAACGCT‑GGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGgcg                                                                 <  2:1257306/140‑1 (MQ=255)                                                                    cACCGAAGAAAAACGCT‑GGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGgcg                                                                 >  2:777820/1‑140 (MQ=255)                                                                      ccGAAGAAAAACGCT‑GGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCa                                                               >  1:865512/1‑140 (MQ=255)                                                                        gaagaaAAACGCT‑GGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCaaa                                                             >  1:1058916/1‑140 (MQ=255)                                                                         aagaaAAACGCT‑GGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGTCGCaaaa                                                            >  1:82107/1‑140 (MQ=255)                                                                         aagaaAAACGCT‑GGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCaaaa                                                            >  2:990387/1‑140 (MQ=255)                                                                                aCGCT‑GGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCtcct                                                     <  2:682121/140‑4 (MQ=255)                                                                                       gATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTgg                                               >  1:864859/1‑140 (MQ=255)                                                                                         tCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGaa                                             >  1:740618/1‑140 (MQ=255)                                                                                          ccAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAg                                            <  1:1430721/140‑1 (MQ=255)                                                                                           cAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAgg                                           <  1:317012/140‑1 (MQ=255)                                                                                           cAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAgg                                           <  1:700030/140‑1 (MQ=255)                                                                                                 cGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGa                                     <  2:812045/140‑1 (MQ=255)                                                                                                 cGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGa                                     >  2:990510/1‑140 (MQ=255)                                                                                                 cGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGa                                     <  1:442906/140‑1 (MQ=255)                                                                                                  gTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGAc                                    >  2:933068/1‑140 (MQ=255)                                                                                                   tATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGAcg                                   >  1:1466786/1‑140 (MQ=255)                                                                                                   tATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGAcg                                   >  2:65719/1‑140 (MQ=255)                                                                                                   tATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGAcg                                   >  1:232918/1‑140 (MQ=255)                                                                                                   tATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGAcg                                   >  2:314274/1‑140 (MQ=255)                                                                                                   tATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGAcg                                   >  2:371744/1‑140 (MQ=255)                                                                                                   tATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGAcg                                   >  1:850752/1‑140 (MQ=255)                                                                                                      cGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGt                                <  1:933068/140‑1 (MQ=255)                                                                                                         gTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTaa                             >  2:154255/1‑140 (MQ=255)                                                                                                          tCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAAt                            >  1:262940/1‑140 (MQ=255)                                                                                                            tGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATcc                          >  2:1186358/1‑140 (MQ=255)                                                                                                             ggTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATccc                         <  2:232918/140‑1 (MQ=255)                                                                                                             ggTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATccc                         <  2:262940/140‑1 (MQ=255)                                                                                                              gTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCg                        <  1:65719/140‑1 (MQ=255)                                                                                                              gTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCg                        >  1:697834/1‑140 (MQ=255)                                                                                                              gTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCg                        >  1:916307/1‑140 (MQ=255)                                                                                                               tCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGa                       <  1:444507/140‑1 (MQ=255)                                                                                                               tCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGa                       <  2:916307/140‑1 (MQ=255)                                                                                                                cgcgcgACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGAt                      <  1:777820/140‑1 (MQ=255)                                                                                                                 gcgcgACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATg                     <  2:729571/140‑1 (MQ=255)                                                                                                                 gcgcgACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATg                     <  1:1186358/140‑1 (MQ=255)                                                                                                                 gcgcgACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATg                     <  2:638079/140‑1 (MQ=255)                                                                                                                  cgcgACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGtt                               <  2:1550863/129‑1 (MQ=255)                                                                                                                  cgcgACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGtt                               >  1:1550863/1‑129 (MQ=255)                                                                                                                     gACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTa                 <  1:876064/140‑1 (MQ=255)                                                                                                                        tttCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAag              <  1:865290/140‑1 (MQ=255)                                                                                                                        tttCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAag              <  1:154255/140‑1 (MQ=255)                                                                                                                                 cGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATcc     >  1:873591/1‑140 (MQ=255)                                                                                                                                 cGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATcc     >  2:1000454/1‑140 (MQ=255)                                                                                                                                   aaGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCGc   >  1:946233/1‑140 (MQ=255)                                                                                                                                   aaGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCGc   >  2:1146759/1‑140 (MQ=255)                                                                                                                                    agagCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCGcc  <  2:1466786/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        AGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCT‑GGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCGCC  >  NC_000913/759169‑759438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑