Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,328,928	C→G	R598R (CGC→CGG)	crfC →	clamp‑binding sister replication fork colocalization protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,328,928	0	C	G	100.0%	319.7 / NA	80	R598R (CGC→CGG)	crfC	clamp‑binding sister replication fork colocalization protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (39/41);  total (39/41)

ATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGTTTCGTCCTGGGTTTCATTTTCCTGTTTTTCACGGCGTAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGCCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACAGTGTGTTGCAA  >  NC_000913/4328794‑4329063                                                                                                                                       |                                                                                                                                        aTTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGTTTCGTCCTGGGTTTCATTTTCCTGTTTTTCACGGCGTAGTTCCCCCCTTCACCACTCGCCAGCTGTTCGGTGAGGCCTTTCCGCGCCAGGa                                                                                                                                    >  2:1372747/1‑140 (MQ=255) aTTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGTTTCGTCCTGGGTTTCATTTTCCTGTTTTTCACGGCGTAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGa                                                                                                                                    <  2:797306/140‑1 (MQ=255)      ccccATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGTTTCGTCCTGGGTTTCATTTTCCTGTTTTTCACGGCGTAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCaa                                                                                                                               >  1:512605/1‑140 (MQ=255)       cccATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGTTTCGTCCTGGGTTTCATTTTCCTGTTTTTCACGGCGTAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAAc                                                                                                                              >  1:557579/1‑140 (MQ=255)       cccATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGTTTCGTCCTGGGTTTCATTTTCCTGTTTTTCACGGCGTAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAAc                                                                                                                              >  1:98605/1‑140 (MQ=255)       cccATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGTTTCGTCCTGGGTTTCATTTTCCTGTTTTTCACGGCGTAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAAc                                                                                                                              >  2:737243/1‑140 (MQ=255)       cccATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGTTTCGTCCTGGGTTTCATTTTCCTGTTTTTCACGGCGTAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAAc                                                                                                                              >  2:290361/1‑140 (MQ=255)         cATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGTTTCGTCCTGGGTTTCATTTTCCTGTTTTTCACGGCGTAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACgg                                                                                                                            <  1:1060562/140‑1 (MQ=255)         cATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGTTTCGTCCTGGGTTTCATTTTCCTGTTTTTCACGGCGTAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACgg                                                                                                                            >  1:472816/1‑140 (MQ=255)              aGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGTTTCGTCCTGGGTTTCATTTTCCTGTTTTTCACGGCGTAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACgag                                                                                                                       >  1:1030298/1‑140 (MQ=255)              aGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGTTTCGTCCTGGGTTTCATTTTCCTGTTTTTCACGGCGTAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACgag                                                                                                                       <  2:82622/140‑1 (MQ=255)                caacaaGTCAAAATGGAATTGAGCGAGTCAGGGTTTCGTCCTGGGTTTCATTTTCCTGTTTTTCACGGCGTAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACgagca                                                                                                                     <  2:512605/140‑1 (MQ=255)                   caaGTCAAAATGGAATTGAGCGAGTCAGGGTTTCGTCCTGGGTTTCATTTTCCTGTTTTTCACGGCGTAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACgagcagag                                                                                                                  >  1:654229/1‑140 (MQ=255)                    aaGTCAAAATGGAATTGAGCGAGTCAGGGTTTCGTCCTGGGTTTCATTTTCCTGTTTTTCACGGCGTAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACgagcagagc                                                                                                                 <  2:557579/140‑1 (MQ=255)                     aGTCAAAATGGAATTGAGCGAGTCAGGGTTTCGTCCTGGGTTTCATTTTCCTGTTTTTCACGGCGTAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCaa                                                                                                                               >  1:20524/1‑125 (MQ=255)                     aGTCAAAATGGAATTGAGCGAGTCAGGGTTTCGTCCTGGGTTTCATTTTCCTGTTTTTCACGGCGTAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCaa                                                                                                                               <  2:20524/125‑1 (MQ=255)                     aGTCAAAATGGAATTGAGCGAGTCAGGGTTTCGTCCTGGGTTTCATTTTCCTGTTTTTCACGGCGTAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACgagcagagca                                                                                                                >  2:930147/1‑140 (MQ=255)                     aGTCAAAATGGAATTGAGCGAGTCAGGGTTTCGTCCTGGGTTTCATTTTCCTGTTTTTCACGGCGTAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACgagcagagca                                                                                                                >  2:965442/1‑140 (MQ=255)                       tCAAAATGGAATTGAGCGAGTCAGGGTTTCGTCCTGGGTTTCATTTTCCTGTTTTTCACGGCGTAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCAcg                                                                                                              <  2:654229/140‑1 (MQ=255)                             tGGAATTGAGCGAGTCAGGGTTTCGTCCTGGGTTTCATTTTCCTGTTTTTCACGGCGTAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTa                                                                                                        <  2:1395201/140‑1 (MQ=255)                                 aTTGAGCGAGTCAGGGTTTCGTCCTGGGTTTCATTTTCCTGTTTTTCACGGCGTAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCg                                                                                                    <  2:1346888/140‑1 (MQ=255)                                     aGCGAGTCAGGGTTTCGTCCT‑GGTTTCATTTTCCTGTTTTTCACGGCGTAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACgagcagagc                                                                                                                 <  2:821334/122‑1 (MQ=255)                                     aGCGAGTCAGGGTTTCGTCCT‑GGTTTCATTTTCCTGTTTTTCACGGCGTAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACgagcagagc                                                                                                                 >  1:821334/1‑122 (MQ=255)                                        gAGTCAGGGTTTCGTCCTGGGTTTCATTTTCCTGTTTTTCACGGCGTAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCg                                                                                             <  1:110359/140‑1 (MQ=255)                                          gTCAGGGTTTCGTCCTGGGTTTCATTTTCCTGTTTTTCACGGCGTAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTg                                                                                           >  1:1460859/1‑140 (MQ=255)                                          gTCAGGGTTTCGTCCTGGGTTTCATTTTCCTGTTTTTCACGGCGTAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTg                                                                                           >  2:944239/1‑140 (MQ=255)                                             aGGGTTTCGTCCTGGGTTTCATTTTCCTGTTTTTCACGGCGTAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTgaga                                                                                        <  2:1139650/140‑1 (MQ=255)                                                   tCGTCCTGGGTTTCATTTTCCTGTTTTTCACGGCGTAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACttttt                                                                                  >  2:1508325/1‑140 (MQ=255)                                                    cGTCCTGGGTTTCATTTTCCTGTTTTTCACGGCGTAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTc                                                                                 >  2:1530193/1‑140 (MQ=255)                                                    cGTCCTGGGTTTCATTTTCCTGTTTTTCACGGCGTAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTc                                                                                 >  1:167301/1‑140 (MQ=255)                                                    cGTCCTGGGTTTCATTTTCCTGTTTTTCACGGCGTAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTc                                                                                 >  1:1418509/1‑140 (MQ=255)                                                    cGTCCTGGGTTTCATTTTCCTGTTTTTCACGGCGTAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTc                                                                                 >  2:617409/1‑140 (MQ=255)                                                    cGTCCTGGGTTTCATTTTCCTGTTTTTCACGGCGTAGTTCCCCACTTCAACACTCGCCAGCTG‑TCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTcg                                                                                >  2:58887/1‑140 (MQ=255)                                                       ccTGGGTTTCATTTTCCTGTTTTTCACGGCGTAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTcgcg                                                                              <  1:944239/140‑1 (MQ=255)                                                                     tCCTGTTTTTCACGGCGTAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGc                                                                <  1:965442/140‑1 (MQ=255)                                                                      ccTGTTTTTCACGGCGTAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGcc                                                               <  2:79519/140‑1 (MQ=255)                                                                           ttttCACGGCGTAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACt                                                          <  1:290361/140‑1 (MQ=255)                                                                           ttttCACGGCGTAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACt                                                          <  2:61019/140‑1 (MQ=255)                                                                             ttCACGGCGTAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTgg                                                        >  2:1471106/1‑140 (MQ=255)                                                                                    cGTAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACggg                                                 >  1:1402306/1‑140 (MQ=255)                                                                                      tAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGa                                               >  1:39414/1‑140 (MQ=255)                                                                                        gTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGaaa                                             <  1:930147/140‑1 (MQ=255)                                                                                         ttCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAAt                                            >  2:1015215/1‑140 (MQ=255)                                                                                              cACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACggg                                                 >  1:1392546/1‑130 (MQ=255)                                                                                              cACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACggg                                                 <  2:1392546/130‑1 (MQ=255)                                                                                              cACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGaa                                       <  1:1015215/140‑1 (MQ=255)                                                                                                cTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAAt                                     >  1:437932/1‑140 (MQ=255)                                                                                                cTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAAt                                     >  1:267530/1‑140 (MQ=255)                                                                                                  tCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAATCa                                   <  2:167301/140‑1 (MQ=255)                                                                                                   cAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAAt                                     <  2:998633/137‑1 (MQ=255)                                                                                                   cAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAAt                                     >  1:998633/1‑137 (MQ=255)                                                                                                   cAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAATCAc                                  >  2:515092/1‑140 (MQ=255)                                                                                                       acTCGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAATCACCGAc                              <  1:1372747/140‑1 (MQ=255)                                                                                                          cGCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGa                           <  2:1442502/140‑1 (MQ=255)                                                                                                           gCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGaa                          >  2:1333577/1‑140 (MQ=255)                                                                                                           gCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGaa                          >  2:1300332/1‑140 (MQ=255)                                                                                                           gCCAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGaa                          >  1:74217/1‑140 (MQ=255)                                                                                                            ccAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGaaa                         <  2:1402306/140‑1 (MQ=255)                                                                                                            ccAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGaaa                         <  1:1104285/140‑1 (MQ=255)                                                                                                            ccAGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGaaa                         <  2:1164499/140‑1 (MQ=255)                                                                                                              aGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACg                       <  1:1471106/140‑1 (MQ=255)                                                                                                              aGCTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACg                       <  2:39414/140‑1 (MQ=255)                                                                                                                cTGTTCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGt                     <  2:267530/140‑1 (MQ=255)                                                                                                                   ttCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGa                  >  2:1436035/1‑140 (MQ=255)                                                                                                                   ttCAGTGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGa                  <  1:659788/140‑1 (MQ=255)                                                                                                                      aGTGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTa               <  1:1530193/140‑1 (MQ=255)                                                                                                                      aGTGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTa               <  2:646760/140‑1 (MQ=255)                                                                                                                        tGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACa             <  1:1300332/140‑1 (MQ=255)                                                                                                                        tGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACa             <  2:98605/140‑1 (MQ=255)                                                                                                                        tGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACa             <  1:1519341/140‑1 (MQ=255)                                                                                                                        tGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACa             <  2:1030298/140‑1 (MQ=255)                                                                                                                        tGAAGTCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACa             >  2:1519341/1‑140 (MQ=255)                                                                                                                             tCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACa             <  2:1097269/135‑1 (MQ=255)                                                                                                                             tCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACa             >  1:1097269/1‑135 (MQ=255)                                                                                                                             tCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACAgtgtg        >  1:618552/1‑140 (MQ=255)                                                                                                                             tCATTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACAgtgtg        >  1:642878/1‑140 (MQ=255)                                                                                                                               aTTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACa             <  2:234574/133‑1 (MQ=255)                                                                                                                               aTTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACa             >  1:234574/1‑133 (MQ=255)                                                                                                                               aTTTCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACAGTGTGtt      >  2:1165959/1‑140 (MQ=255)                                                                                                                                 ttCGCGGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTTGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACAGTGTGTTGc    <  2:1097735/140‑1 (MQ=255)                                                                                                                                   cgcgGCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACAGTGTGTTGCaa  <  2:1418509/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        ATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGTTTCGTCCTGGGTTTCATTTTCCTGTTTTTCACGGCGTAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGCCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACAGTGTGTTGCAA  >  NC_000913/4328794‑4329063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑