Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	924,123	T→A	I287N (ATC→AAC)	clpA →	ATP‑dependent Clp protease ATP‑binding subunit ClpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	924,123	0	T	A	100.0%	197.2 / NA	56	I287N (ATC→AAC)	clpA	ATP‑dependent Clp protease ATP‑binding subunit ClpA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (30/26);  total (30/26)

TGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGACAACCTATCAGGAGTTCAGCAACATTTTCG  >  NC_000913/923986‑924257                                                                                                                                          |                                                                                                                                       tGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGAAcc                                                                                                                                      <  2:265194/140‑1 (MQ=255)   aCTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGAACcac                                                                                                                                    <  1:1128724/140‑1 (MQ=255)   aCTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGAACcac                                                                                                                                    <  1:312190/140‑1 (MQ=255)   aCTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGAACcac                                                                                                                                    <  2:368076/140‑1 (MQ=255)   aCTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGAACcac                                                                                                                                    >  1:67002/1‑140 (MQ=255)       tACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGAACCACACCa                                                                                                                                >  2:887287/1‑140 (MQ=255)                   tctcGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGAACCACACCATTATCGGTGCgg                                                                                                                    >  1:1068191/1‑140 (MQ=255)                     tcGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGAACCACACCATTATCGGTGCGGGt                                                                                                                  >  2:970218/1‑140 (MQ=255)                     tcGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGAACCACACCATTATCGGTGCGGGt                                                                                                                  >  1:607408/1‑140 (MQ=255)                        atatCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGAACCACACCATTATCGGTGCGGGTGCa                                                                                                               <  2:859493/140‑1 (MQ=255)                               ttCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGAACCACACCATTATCGGTGCGGGTGCAGCGTCtg                                                                                                        <  2:1068191/140‑1 (MQ=255)                               ttCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGAACCACACCATTATCGGTGCGGGTGCAGCGTCtg                                                                                                        <  2:1026671/140‑1 (MQ=255)                               ttCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGAACCACACCATTATCGGTGCGGGTGCAGCGTCtg                                                                                                        >  1:232895/1‑140 (MQ=255)                               ttCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGAACCACACCATTATCGGTGCGGGTGCAGCGTCtg                                                                                                        >  1:662527/1‑140 (MQ=255)                                   ctGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGAACCACACCATTATCGGTGCGGGTGCAGCGTCtggtgg                                                                                                    >  2:1280513/1‑140 (MQ=255)                                    tGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGAACCACACCATTATCGGTGCGGGTGCAGCGTCTggtggt                                                                                                   >  1:1243293/1‑140 (MQ=255)                                            ggCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGAACCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGa                                                                                           >  1:674253/1‑140 (MQ=255)                                            ggCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGAACCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGa                                                                                           <  2:232895/140‑1 (MQ=255)                                              cacaAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGAACCACACCATTATCGGTGCGGGTGCAGCGTCtg                                                                                                        >  2:1043223/1‑125 (MQ=255)                                              cacaAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGAACCACACCATTATCGGTGCGGGTGCAGCGTCtg                                                                                                        <  1:1043223/125‑1 (MQ=255)                                              cacaAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGAACCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATg                                                                                         >  2:1325356/1‑140 (MQ=255)                                              cacaAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGAACCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATg                                                                                         >  2:1043741/1‑140 (MQ=255)                                                   aaTATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGAACCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCt                                                                                    <  2:607408/140‑1 (MQ=255)                                                     tatCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGAACCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTaa                                                                                  <  1:1047822/140‑1 (MQ=255)                                                     tatCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGAACCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTaa                                                                                  <  2:662527/140‑1 (MQ=255)                                                      atCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGAACCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAAc                                                                                 <  1:887287/140‑1 (MQ=255)                                                       tCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGAACCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAAcc                                                                                >  1:416750/1‑140 (MQ=255)                                                        cgcgGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGAACCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCt                                                                               >  1:579719/1‑140 (MQ=255)                                                             cgACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGAACCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCa                                                                          <  2:864177/140‑1 (MQ=255)                                                                       aaaCGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGAACCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGct                                                                >  2:1339278/1‑140 (MQ=255)                                                                             tttAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGAACCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAg                                                          >  2:415439/1‑140 (MQ=255)                                                                                aaaGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGAACCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCgg                                                       <  2:650391/140‑1 (MQ=255)                                                                                    cGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGAACCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCtctc                                                             >  2:8156/1‑130 (MQ=255)                                                                                    cGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGAACCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCtctc                                                             <  1:8156/130‑1 (MQ=255)                                                                                     gTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGAACCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTaaaa                                                  <  2:1479868/140‑1 (MQ=255)                                                                                      ttGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGAACCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAAt                                                 <  1:1339278/140‑1 (MQ=255)                                                                                        gCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGAACCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTc                                               >  1:221490/1‑140 (MQ=255)                                                                                                        cAGGACACTAACAGCATCCTGTTTATTGATGAGAACCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGAc                               <  2:630139/140‑1 (MQ=255)                                                                                                        cAGGACACTAACAGCATCCTGTTTATTGATGAGAACCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGAc                               <  1:1280513/140‑1 (MQ=255)                                                                                                           gACACTAACAGCATCCTGTTTATTGATGAGAACCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGAc                               <  2:1180985/137‑1 (MQ=255)                                                                                                           gACACTAACAGCATCCTGTTTATTGATGAGAACCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGAc                               >  1:1180985/1‑137 (MQ=255)                                                                                                           gACACTAACAGCATCCTGTTTATTGATGAGAACCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGACAAc                            <  1:415439/140‑1 (MQ=255)                                                                                                                tAACAGCATCCTGTTTATTGATGAGAACCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGAc                               >  1:1023068/1‑132 (MQ=255)                                                                                                                tAACAGCATCCTGTTTATTGATGAGAACCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGAc                               <  2:1023068/132‑1 (MQ=255)                                                                                                                     gCATCCTGTTTATTGATGAGAACCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGACAACCTATCAGGAg                  >  1:1177288/1‑140 (MQ=255)                                                                                                                     gCATCCTGTTTATTGATGAGAACCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGACAACCTATCAGGAg                  >  1:1168147/1‑140 (MQ=255)                                                                                                                      cATCCTGTTTATTGATGAGAACCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGACAACCTATCAGGAg                  >  2:1456468/1‑139 (MQ=255)                                                                                                                      cATCCTGTTTATTGATGAGAACCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGACAACCTATCAGGAg                  <  1:1456468/139‑1 (MQ=255)                                                                                                                      cATCCTGTTTATTGATGAGAACCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGACAACCTATCAGGAGt                 >  2:1036173/1‑140 (MQ=255)                                                                                                                       aTCCTGTTTATTGATGAGAACCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGACAACCTATCAGGAGtt                >  1:1517129/1‑140 (MQ=255)                                                                                                                          cTGTTTATTGATGAGAACCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGACAACCTATCAGGAGTTCAg             >  1:265388/1‑140 (MQ=255)                                                                                                                             tttATTGATGAGAACCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGACAACCTATCAGGAGTTCAGCaa          >  1:1169588/1‑140 (MQ=255)                                                                                                                                aTTGATGAGAACCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGACAACCTATCAGGAGTTCAGCAACAt       >  1:848039/1‑140 (MQ=255)                                                                                                                                  tgatgaGAACCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGACAACCTATCAGGAGTTCAGCAACAttt     <  2:1168147/140‑1 (MQ=255)                                                                                                                                    atgaGAACCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGACAACCTATCAGGAGTTCAGCAACATTTTc   <  2:416750/140‑1 (MQ=255)                                                                                                                                     tgaGAACCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGACAACCTATCAGGAGTTCAGCAACATTTTCg  >  2:118307/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       TGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGACAACCTATCAGGAGTTCAGCAACATTTTCG  >  NC_000913/923986‑924257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑