Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,149,855	A→G	D43G (GAC→GGC)	fabD →	[acyl‑carrier‑protein] S‑malonyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,149,855	0	A	G	100.0%	185.4 / NA	49	D43G (GAC→GGC)	fabD	[acyl‑carrier‑protein] S‑malonyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/27);  total (22/27)

TAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGACCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATAAAACCTGGCAAACTCAGCCTGCGCTGTTGACTGCATCTGTTGCGCTGTATCGCGTATGGCAGCAGCAGGGCGGTAAAGCA  >  NC_000913/1149722‑1149979                                                                                                                                      |                                                                                                                             tAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGGCCtgtg                                                                                                                        >  2:605146/1‑140 (MQ=255)  aaaaCATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGGCCTGTgg                                                                                                                       >  2:472735/1‑140 (MQ=255)    aacgttaCGTAATTTGTTTTTGTGTTCCCTGTTCAGGGTTCTCAGTCTGTTGGAATGGTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAGCGTTTGCTGAAGCTTCTGCGGCGTTGGGCTACGGTCTGTGGgc                                                                                                                     <  2:1552980/134‑1 (MQ=255)     aCATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGGCCTGTGGgcg                                                                                                                    <  2:108106/140‑1 (MQ=255)         gACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGGCCTGTGGGCGCTgc                                                                                                                >  1:1526525/1‑139 (MQ=255)         gACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGGCCTGTGGGCGCTGa                                                                                                                >  1:293054/1‑140 (MQ=255)                   gCATTTGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGGCCTGTGGGCGCTGACCCAGCAggg                                                                                                      <  1:606708/140‑1 (MQ=255)                      tttGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGGCCTGTGGGCGCTGACCCAGCAGGGGcc                                                                                                   <  2:1309215/140‑1 (MQ=255)                      tttGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGGCCTGTGGGCGCTGACCCAGCAGGGGcc                                                                                                   <  1:960872/140‑1 (MQ=255)                          tgtTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGGCCTGTGGGCGCTGACCCAGCAGGGGCCAGCt                                                                                               >  1:1102089/1‑140 (MQ=255)                                 tGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGGCCTGTGGGCGCTGACCCAGCAGGGGCCAGCTgaagaa                                                                                         <  2:1228576/139‑1 (MQ=255)                                 tGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGGCCTGTGGGCGCTGACCCAGCAGGGGCCAGCTgaagaa                                                                                         >  1:1228576/1‑139 (MQ=255)                                        ggTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGGCCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATaa                                                                                 >  1:129098/1‑140 (MQ=255)                                        ggTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGGCCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATaa                                                                                 >  1:444488/1‑140 (MQ=255)                                        ggTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGGCCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATaa                                                                                 >  1:501846/1‑140 (MQ=255)                                        ggTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGGCCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATaa                                                                                 >  2:1187485/1‑140 (MQ=255)                                        ggTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGGCCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATaa                                                                                 >  2:1162482/1‑140 (MQ=255)                                           tctcAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGGCCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATAAAAc                                                                              <  1:605146/140‑1 (MQ=255)                                                   cGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGGCCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATAAAACCTGGCaaa                                                                      >  1:1155688/1‑140 (MQ=255)                                                    gTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGGCCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATAAAACCTGGCAAAc                                                                     <  2:293054/140‑1 (MQ=255)                                                       ggAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGGCCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATAAAACCTGGCAAACTCa                                                                  <  1:632638/140‑1 (MQ=255)                                                             ctggctgATATGGCGGCGGGCTATCCAATTGTCGCAGGAACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGGCCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGGACTGGATAAAACCTGGCAAACTCCGGCTgc                                                            >  2:1276741/1‑140 (MQ=255)                                                              tggctgATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGGCCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATAAAACCTGGCAAACTCAGCCTgcg                                                           <  2:444488/140‑1 (MQ=255)                                                                gctgATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGGGCTGGGCTACGGCCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATAAAACCTGGCAAACTCAGc                                                                >  2:482279/1‑133 (MQ=255)                                                                gctgATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGGCCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATAAAACCTGGCAAACTCAGc                                                                <  1:482279/133‑1 (MQ=255)                                                                  tgATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGGCCTGTGGGCGCTGACCCAGTA‑GGGCCAGCTGAAGAACTGAATAAAACCTGGCAAACTCAGCCTGCGCTGtt                                                      >  1:976066/1‑140 (MQ=255)                                                                    atatGGCGGCGAGTTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGGCCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATAAAACCTGGCAAACTCAGCCTGCGCTGTTg                                                     <  2:66698/140‑1 (MQ=255)                                                                          cggcgAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGGCCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATAAAACCTGGCAAACTCAGCCTgcg                                                           <  2:1053526/128‑1 (MQ=255)                                                                          cggcgAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGGCCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATAAAACCTGGCAAACTCAGCCTgcg                                                           >  1:1053526/1‑128 (MQ=255)                                                                          cggcgAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGGCCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATAAAACCTGGCAAACTCAGCCTGCGCTGTTGACTGCa                                               >  1:1304209/1‑140 (MQ=255)                                                                             cgAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGGCCTGTGGGCGCTGACCCAGTA‑GGGCCAGCTGAAGAACTGAATAAAACCTGGCAAACTCAGCCTGCGCTGTTGACTGCATCTg                                           <  2:976066/140‑1 (MQ=255)                                                                              gAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGGCCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATAAAACCTGGCAAACTCAGCCTGCGCTGTTGACTGCATCTg                                           <  1:1187485/140‑1 (MQ=255)                                                                              gAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGGCCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATAAAACCTGGCAAACTCAGCCTGCGCTGTTGACTGCATCTg                                           <  2:512778/140‑1 (MQ=255)                                                                              gAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGGCCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATAAAACCTGGCAAACTCAGCCTGCGCTGTTGACTGCATCTg                                           >  2:15488/1‑140 (MQ=255)                                                                              gAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGGCCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATAAAACCTGGCAAACTCAGCCTGCGCTGTTGACTGCATCTg                                           >  1:512778/1‑140 (MQ=255)                                                                               aGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGGCCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATAAAACCTGGCAAACTCAGCCTGCGCt                                                         <  1:88146/125‑1 (MQ=255)                                                                               aGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGGCCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATAAAACCTGGCAAACTCAGCCTGCGCt                                                         >  2:88146/1‑125 (MQ=255)                                                                                 cTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGGCCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATAAAACCTGGCAAACTCAGCCTGCGCTGTTGACTGCATCTGTTg                                        >  1:1094926/1‑140 (MQ=255)                                                                                   aTCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGGCCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATAAAACCTGGCAAACTCAGCCTGCGCTGTTGACTGCATCTGTTgcg                                      <  1:472735/140‑1 (MQ=255)                                                                                   aTCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGGCCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATAAAACCTGGCAAACTCAGCCTGCGCTGTTGACTGCATCTGTTgcg                                      <  1:119732/140‑1 (MQ=255)                                                                                   aTCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGGCCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATAAAACCTGGCAAACTCAGCCTGCGCTGTTGACTGCATCTGTTgcg                                      <  1:270974/140‑1 (MQ=255)                                                                                   aTCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGGCCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATAAAACCTGGCAAACTCAGCCTGCGCTGTTGACTGCATCTGTTgcg                                      <  2:426055/140‑1 (MQ=255)                                                                                   aTCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGGCCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATAAAACCTGGCAAACTCAGCCTGCGCTGTTGACTGCATCTGTTgcg                                      <  2:129098/140‑1 (MQ=255)                                                                                   aTCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGGCCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATAAAACCTGGCAAACTCAGCCTGCGCTGTTGACTGCATCTGTTgcg                                      >  2:1204078/1‑140 (MQ=255)                                                                                         ttGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGGCCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATAAAACCTGGCAAACTCAGCCTGCGCTGTTGACTGCATCTg                                           <  1:741572/129‑1 (MQ=255)                                                                                         ttGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGGCCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATAAAACCTGGCAAACTCAGCCTGCGCTGTTGACTGCATCTg                                           >  2:741572/1‑129 (MQ=255)                                                                                              gaagaaACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGGCCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATAAAACCTGGCAAACTCAGCCTGCGCTGTTGACTGCATCTGTTgcg                                      >  2:766577/1‑129 (MQ=255)                                                                                              gaagaaACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGGCCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATAAAACCTGGCAAACTCAGCCTGCGCTGTTGACTGCATCTGTTgcg                                      <  1:766577/129‑1 (MQ=255)                                                                                                         tGCTGAAGCTTCTGCGGCGCTGGGCTACGGCCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATAAAACCTGGCAAACTCAGCCTGCGCTGTTGACTGCATCTGTTGCGCTGTATCGCGTATGgcagcagc                >  2:505222/1‑140 (MQ=255)                                                                                                             gAAGCTTCTGCGGCGCTGGGCTACGGCCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATAAAACCTGGCAAACTCAGCCTGCGCTGTTGACTGCATCTGTTGCGCTGTATCGCGTATGGCAGCAGCAggg            <  2:1102089/140‑1 (MQ=255)                                                                                                                      gcggcgCTGGGCTACGGCCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATAAAACCTGGCAAACTCAGCCTGCGCTGTTGACTGCATCTGTTGCGCTGTATCGCGTATGGCAGCAGCAGGGCGGTAAAGc   <  1:15488/140‑1 (MQ=255)                                                                                                                       cggcgCTGGGCTACGGCCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATAAAACCTGGCAAACTCAGCCTGCGCTGTTGACTGCATCTGTTGCGCTGTATCGCGTATGGCAGCAGCAGGGCGGTAAAGCa  <  2:1304209/140‑1 (MQ=255)                                                                                                                          cgcTGGGCTACGGCCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATAAAACCTGGCAAACTCAGCCTGCGCTGTTGACTGCATCTGTTGCGCTGTATCGCGTATGGCAGCAGCAggg            <  1:524704/127‑1 (MQ=255)                                                                                                                          cgcTGGGCTACGGCCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATAAAACCTGGCAAACTCAGCCTGCGCTGTTGACTGCATCTGTTGCGCTGTATCGCGTATGGCAGCAGCAggg            >  2:524704/1‑127 (MQ=255)                                                                                                                                      |                                                                                                                             TAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGACCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATAAAACCTGGCAAACTCAGCCTGCGCTGTTGACTGCATCTGTTGCGCTGTATCGCGTATGGCAGCAGCAGGGCGGTAAAGCA  >  NC_000913/1149722‑1149979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑