Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	445,558	0	A	C	25.5%	96.7 / 16.9	47	V370G (GTA→GGA)	yajR	putative major facilitator superfamily transport protein YajR
Reads supporting (aligned to +/- strand):  ref base A (23/12);  new base C (0/12);  total (23/24)
Fisher's exact test for biased strand distribution p-value = 7.76e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAA‑ACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGG  >  NC_000913/445424‑445696                                                                                                                                       |                                                                                                                                            aaCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATAccccc                                                                                                                                        <  2:796477/140‑1 (MQ=255)  aCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCt                                                                                                                                       <  2:904706/140‑1 (MQ=255)  aCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCt                                                                                                                                       <  2:1624501/140‑1 (MQ=255)  aCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCt                                                                                                                                       <  2:1031160/140‑1 (MQ=255)   cGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTg                                                                                                                                      <  1:1337535/140‑1 (MQ=255)         tttAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTc                                                                                                                                <  1:570642/140‑1 (MQ=255)              cGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAA‑ACa                                                                                                                          <  2:572187/140‑1 (MQ=255)              cGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAA‑ACa                                                                                                                          <  1:945758/140‑1 (MQ=255)              cGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACCGTCAGACACACTTCGGCCAGGATTTCGCCAGCGAGAAATACCCCCTCACCGTCaa‑aaa                                                                                                                          >  2:1577057/1‑138 (MQ=255)                  tCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAA‑ACATGcc                                                                                                                      <  1:410891/140‑1 (MQ=255)                   cGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGa                                                                                                                                     <  1:1075483/125‑1 (MQ=255)                   cGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGa                                                                                                                                     >  2:1075483/1‑125 (MQ=255)                         cGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGGCTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAAAACCCCCTGACCCTCAA‑ACATGCCGCTAATc                                                                                                               >  2:1368124/1‑140 (MQ=255)                                      cGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTTATGGGACTGGCGACTGTCAGCCACACCGCCGCCCGCCATGCGCCCGCGGGAAAAACCCCCCTACCGTCCA‑AAAAGCCGTTTATTCAGcccccccacc                                                                                                  >  2:414900/1‑136 (MQ=255)                                         gATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAAAACCCCCCGACCGTCAA‑ACATGCCGGTAATCCAGCCGCCCAGCGAAc                                                                                               >  2:140800/1‑140 (MQ=255)                                                   cgcAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAA‑ACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATcgc                                                                                     <  1:785950/140‑1 (MQ=255)                                                   cgcAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACCGTCAGCCACACTGCGGCCAGCATTTCGCCAGCGAGAAAAACCCCCTGACCGTCAA‑ACAAGCCGTTAATCCAGCCCCCCCGCGAACCCCCCAAcgc                                                                                     >  2:1436902/1‑140 (MQ=255)                                                     cAACCTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGCCCGTCAA‑ACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATcgcca                                                                                   <  1:1412718/140‑1 (MQ=255)                                                                  tACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTTCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAA‑AAAAGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCCAaaaaaag                                                                      >  2:831563/1‑137 (MQ=255)                                                                        ggTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAA‑ACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCtggt                                                                <  1:607925/140‑1 (MQ=255)                                                                           tCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAAAACCCCCCGACCGTCAA‑ACATGCCGTTAATCCCGCCCCCCCGCGAACCGCCAATCGCCCCCCCCAGAAAATGGCtgggggg                                                             >  2:1086534/1‑139 (MQ=255)                                                                                 aTGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGGGAAATACCCCCCGACCGTCAA‑ACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACCCCAAGAAAATGGCTGGGGGGGGaaaa                                                       >  2:1768946/1‑139 (MQ=255)                                                                                      ccTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCGGCGAAAAATCCCCCCTGACCGTCAA‑ACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCa                                                  <  2:1598614/139‑1 (MQ=255)                                                                                      aCTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAA‑ACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCa                                                  >  2:1060759/1‑140 (MQ=255)                                                                                      aCTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAA‑ACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCa                                                  >  2:102939/1‑140 (MQ=255)                                                                                       cTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAA‑ACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCAt                                                 >  1:1598614/1‑140 (MQ=255)                                                                                        tGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAA‑ACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATc                                                >  2:972401/1‑140 (MQ=255)                                                                                         ggCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAA‑ACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCg                                               >  1:1812185/1‑140 (MQ=255)                                                                                         ggCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAA‑ACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCg                                               >  1:271641/1‑140 (MQ=255)                                                                                          gCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAA‑ACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGc                                              >  2:89574/1‑140 (MQ=255)                                                                                           cGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAA‑ACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGcc                                             >  1:1237437/1‑140 (MQ=255)                                                                                           cGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAA‑ACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGcc                                             >  1:1619512/1‑140 (MQ=255)                                                                                            gACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGCCCGTCAA‑ACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCg                                            <  1:1436902/140‑1 (MQ=255)                                                                                              gggccAGCCCCCCTGCGGCCAGCATTGCCCCAGCGAAAAATCCCCCCTACCCGTCAA‑ACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTa                                          <  2:782515/136‑1 (MQ=255)                                                                                              cTGTCAGCCACCCTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAA‑ACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTa                                          <  1:1768946/140‑1 (MQ=255)                                                                                              cTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAA‑ACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTa                                          <  1:1502079/140‑1 (MQ=255)                                                                                              cTGTCAGCCACACTGCGGCCAGCATGGCGCCAGCGAAAAATCCCCCCTGACCGTCAA‑ACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTa                                          <  2:966958/140‑1 (MQ=255)                                                                                               tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAA‑ACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTAc                                         >  1:557994/1‑140 (MQ=255)                                                                                               tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAA‑ACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTAc                                         >  2:823131/1‑140 (MQ=255)                                                                                               tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAA‑ACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTAc                                         >  2:1055306/1‑140 (MQ=255)                                                                                                           tGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAA‑ACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAAccct                             >  1:782515/1‑138 (MQ=255)                                                                                                           tGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAA‑ACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctg                             >  1:1165450/1‑140 (MQ=255)                                                                                                           tGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAA‑ACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctg                             >  1:793080/1‑140 (MQ=255)                                                                                                           gggggCCAGATTGGCGCCAGCGAGAAATCCCCCCTGACCGTCAA‑ACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctg                             <  1:414900/137‑1 (MQ=255)                                                                                                               gCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAA‑ACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTgg                         <  1:140800/140‑1 (MQ=255)                                                                                                               gCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAA‑ACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTgg                         >  1:754420/1‑140 (MQ=255)                                                                                                               gCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAA‑ACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTgg                         >  1:672295/1‑140 (MQ=255)                                                                                                               gCCAGCATGGCCCCAGCGAAAAATCCCCCCGGCCCGCAAA‑ACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTgg                         <  2:1237437/140‑1 (MQ=255)                                                                                                                cggactttGCGCAAGCGA‑AAACGCCCCCTGACCCTTAACACAGCCCGTTACTCCAGCAGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGc                        <  2:108490/134‑1 (MQ=255)                                                                                                                  ggCATTCCGCCAGCGAAAAATCCCCCTTGCCCGTCAA‑CCATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGa                      <  2:633850/139‑1 (MQ=255)                                                                                                                  aGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAA‑ACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGa                      <  1:1060759/140‑1 (MQ=255)                                                                                                                  aGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAA‑ACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGa                      <  1:89574/140‑1 (MQ=255)                                                                                                                  aGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAA‑ACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGa                      >  1:825956/1‑140 (MQ=255)                                                                                                                       tGCGCCAGCGAGAAATACCCCCTGACCGTCAA‑ACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCtt                 >  1:108490/1‑140 (MQ=255)                                                                                                                        gcgcCAGCGAGAAATACCCCCTGACCGTCAA‑ACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt                <  1:972401/140‑1 (MQ=255)                                                                                                                        gcgcCAGCGAAAAATCCCCCTTGCCCGTAAA‑ACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt                <  2:1505857/140‑1 (MQ=255)                                                                                                                        gcgcCAGCGAAAAATCCCCCCTGCCCGACAA‑ACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt                <  2:618113/140‑1 (MQ=255)                                                                                                                        gcgcCAGCGAAAAATCCCCCCGGCCCGTAAA‑ACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt                <  2:1165450/140‑1 (MQ=255)                                                                                                                        gccccAGCGAAAAATCCCCCCTGACCGTAAA‑ACATGCCGTTAACCCAGCCGCCCAGCAAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt                <  2:825956/137‑1 (MQ=255)                                                                                                                            cAGCGAGAAATCCCCCCTGACCGTAAA‑ACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTg            <  2:160962/140‑1 (MQ=255)                                                                                                                             aGCGAGAAATACCCCCTGACCGTCAA‑ACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGa           >  2:900916/1‑140 (MQ=255)                                                                                                                              gggaaaaaTCCCCCCAGACCGTAAA‑ACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGAt          <  2:1619512/135‑1 (MQ=255)                                                                                                                              gCGAGAAATACCCCCTGACCGTCAA‑ACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGAt          >  1:1706571/1‑140 (MQ=255)                                                                                                                                gagaAATACCCCCTGACCGTCAA‑ACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATaa        >  1:664095/1‑140 (MQ=255)                                                                                                                                  aaaaTCCCCCCGGCCCGTAAA‑ACATGCCGTTAACCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGt      <  2:557994/139‑1 (MQ=255)                                                                                                                                   aaaTCCCCCCTGACCGTCAA‑ACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTg     <  1:1408146/140‑1 (MQ=255)                                                                                                                                      tACCCCCTGACCGTCAA‑ACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAgg  >  2:474371/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                            AACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAA‑ACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGG  >  NC_000913/445424‑445696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑