Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,001,918	0	A	C	28.1%	110.2 / 21.5	57	intergenic (‑129/+192)	fliA/fliC	RNA polymerase, sigma 28 (sigma F) factor/flagellar filament structural protein
Reads supporting (aligned to +/- strand):  ref base A (37/4);  new base C (0/16);  total (37/20)
Fisher's exact test for biased strand distribution p-value = 8.37e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTT‑GGGGGTTACAGGTAAATT‑CCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGC  >  NC_000913/2001779‑2002057                                                                                                                                              |                                                                                                                                             agTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTT‑GGGGGTTACAGGTAAATT‑CCAGGCAGaaaaaaa                                                                                                                                              >  2:178477/1‑140 (MQ=255)  gtaaaTCCACAATAAACGGCCCTGCGTTATAGGATTTATCGGAATGATTATCCTTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTT‑GGGGTTAACAGTTAAATC‑CCAGCCA‑AAAAAAA‑cc                                                                                                                                           <  2:1079889/137‑1 (MQ=255)   tGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTT‑GGGGGTTACAGGTAAATT‑CCAGGCAGAAAAAAA‑cc                                                                                                                                           >  1:1468748/1‑140 (MQ=255)      aTTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTT‑GGGGGTTACAGGTAAATT‑CCAGGCAGAAAAAAA‑CCccg                                                                                                                                        >  1:971243/1‑140 (MQ=255)                      ccTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTT‑GGGGGTTACAGGTAAATT‑CCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGc                                                                                                                        >  1:445738/1‑140 (MQ=255)                        tGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGGTTACAGGTAAATT‑CCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCa                                                                                                                       >  2:317115/1‑140 (MQ=255)                        tGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTT‑GGGGGTTACAGGTAAATT‑CCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCAc                                                                                                                      >  1:1285438/1‑140 (MQ=255)                          cGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTT‑GGGGGTTACAGGTAAATT‑CCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGGAGCACGt                                                                                                                    >  1:1291524/1‑140 (MQ=255)                               tatGATTTATGGGGATGATTATCCGTTTCTGCGGGGTTTTTAATCGGACGATTAGTGGGTAAAAGGAGGGGTTTTTT‑GGGGGTTACAGGTAAATT‑CCAGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTg                                                                                                               <  2:968154/140‑1 (MQ=255)                                    gTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTT‑GGGGGTTACAGGTAAATT‑CCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACaaa                                                                                                          >  2:825613/1‑140 (MQ=255)                                    gTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTT‑GGGGGTTACAGGTAAATT‑CCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACaaa                                                                                                          >  1:364166/1‑140 (MQ=255)                                       aTCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTT‑GGGGGTTACAGGTAAATT‑CCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGCAGCACGTTGCTGAcctgtct                                                                                                       >  1:1529567/1‑134 (MQ=255)                                       aTCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTT‑GGGGGTTACAGGTAAATT‑CCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTg                                                                                                       >  2:764245/1‑140 (MQ=255)                                       aTCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTT‑GGGGGTTACAGGTAAATT‑CCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTg                                                                                                       >  1:8616/1‑140 (MQ=255)                                       aTCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTT‑GGGGGTTACAGGTAAATT‑CCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTg                                                                                                       >  2:841242/1‑140 (MQ=255)                                       aTCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTT‑GGGGGTTACAGGTAAATT‑CCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTg                                                                                                       >  1:1389377/1‑140 (MQ=255)                                         cGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTT‑GGGGGTTACAGGTAAATT‑CCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTgcg                                                                                                     >  1:832466/1‑140 (MQ=255)                                         cGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGGGAAATGAGGGGTTATTT‑GGGGGTTACAGGTAAATT‑CCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTgcg                                                                                                     >  2:1629876/1‑140 (MQ=255)                                             aTGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTT‑GGGGGTTACAGGTAAATT‑CCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCttt                                                                                                 >  2:967045/1‑140 (MQ=255)                                                cagaTCCTTTTTGGCAGGTTTTTAAATGGGACGTTTGGTGGGTAAAAGGGGGGTTTTTTG‑GGGGGTTACAGGAAAATT‑CCAGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATg                                                                                              <  2:1085915/137‑1 (MQ=255)                                                         ttCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTT‑GGGGGTTACAGGTAAATT‑CCAGGCAGAAAAAAA‑CCCCGCCGGTG‑‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgc                                                                                   >  2:1162264/1‑140 (MQ=255)                                                          tCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTT‑GGGGGTTACAGGTAAATT‑CCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATg                                                                                    >  1:1010861/1‑140 (MQ=255)                                                          tCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTT‑GGGGGTTACAGGTAAATT‑CCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATg                                                                                    >  2:1589999/1‑140 (MQ=255)                                                              cAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTT‑GGGGGTTACAGGTAAATT‑CCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgcctg                                                                                >  1:1085915/1‑137 (MQ=255)                                                                 ggTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTT‑GGGGGTTACAGGTAAATT‑CCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTa                                                                             >  2:1066041/1‑140 (MQ=255)                                                                  gTTTTTAATCGGGAGATTAAGTGGTGAAAGTGGGGGTTTTTT‑GGGGGTTTAAAGTAAATTCCCAGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTa                                                                             <  2:1291524/140‑1 (MQ=255)                                                                  gTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTT‑GGGGGTTACAGGTAAATT‑CCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTaa                                                                            >  1:1073509/1‑140 (MQ=255)                                                                  gTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTT‑GGGGGTTACAGGTAAATT‑CCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTaa                                                                            >  2:502392/1‑140 (MQ=255)                                                                   tttttAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTT‑GGGGGTTACAGGTAAATT‑CCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTaaa                                                                           >  2:348476/1‑140 (MQ=255)                                                                    ttttAACCGGAGGATTAGGGGGTGAAATGAGGGGTTATTT‑GGGGGTTACAGGAAAATT‑CCAGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAAc                                                                          <  2:537544/140‑1 (MQ=255)                                                                     tttAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTT‑GGGGGTTACAGGTAAATT‑CCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACg                                                                         >  1:661772/1‑140 (MQ=255)                                                                      ttAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTT‑GGGGGTTACAGGTAAATT‑CCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGc                                                                        >  1:969542/1‑140 (MQ=255)                                                                             gACGATTAGTGGGTGAAATGAGGGGTTATTT‑GGGGGTTACAGGTAAATT‑CCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATcc                                                                 >  2:1228984/1‑140 (MQ=255)                                                                             gACGATTAGTGGGTGAAATGAGGGGTTATTT‑GGGGGTTACAGGTAAATT‑CCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATcc                                                                 >  2:1314039/1‑140 (MQ=255)                                                                             gACGATTAGTGGGTGAAATGAGGGGTTATTT‑GGGGGTTACAGGTAAATT‑CCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATcc                                                                 >  2:1222374/1‑140 (MQ=255)                                                                             gACGATTAGTGGGTGAAATGAGGGGTTATTT‑GGGGGTTACAGGTAAATT‑CCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATcc                                                                 >  1:537544/1‑140 (MQ=255)                                                                                gATTGGGGGGTAAAAGGGGGGTTTATTG‑GGGGGTTACAGGTAAATT‑CCAGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                              <  2:442308/140‑1 (MQ=37)                                                                                   tAGTGGGTGAAAGGAGGGTTTATTT‑GGGGGTTACAGGTAAATT‑CCAGGAAGAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTa                                                           <  2:661772/140‑1 (MQ=255)                                                                                        ggTGAAATGAGGGGTTATTT‑GGGGGTTACAGGTAAATT‑CCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaaa                                                      >  1:442308/1‑140 (MQ=255)                                                                                            aaaTGGGGGTTTATTG‑GGGGGTTACAGGAAAATC‑CCAGGCAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAtgt                                                  <  2:355720/140‑1 (MQ=37)                                                                                              aTGGGGGGTTATTG‑GGGGGTTACAGGAAAATT‑CCGGGAAGAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGc                                                <  2:1285438/140‑1 (MQ=38)                                                                                              aTGAGGGTTTTTGG‑GGGGGTTACAGGTAAATC‑CCAGGCAGAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGc                                                <  2:8616/140‑1 (MQ=38)                                                                                                  ggggTTATTT‑GGGGGTTACAGGTAAATT‑CCAGGCAAAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAAt                                            <  1:1228984/140‑1 (MQ=255)                                                                                                      ttATTT‑GGGGGTTACAGGTAAATT‑CCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCaa                                        >  1:1542827/1‑140 (MQ=255)                                                                                                        aTTT‑GGGGGTTACAGGTAAATT‑CCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATa                                      >  2:1149873/1‑140 (MQ=255)                                                                                                            ggggggTTACAGGTAAATC‑CCAGGAAGAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAAt                                   <  1:178477/139‑1 (MQ=255)                                                                                                            ggggggTTACAGGTAAATC‑CCAGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAAt                                   <  2:971243/139‑1 (MQ=255)                                                                                                             gggggTTAGAGG‑AAATT‑CCCGGAAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAGATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAAtt                                  <  2:969542/140‑1 (MQ=25)                                                                                                               gggTTACAGGTAAATT‑CCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGc                                <  1:1222374/140‑1 (MQ=255)                                                                                                                    aCAGGTAAATT‑CCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTc                           >  2:1750031/1‑140 (MQ=255)                                                                                                                    aCAGGTAAATT‑CCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTc                           >  2:1413690/1‑140 (MQ=255)                                                                                                                    aCAGGTAAATT‑CCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTc                           >  1:532200/1‑140 (MQ=255)                                                                                                                     cAGGTAAATT‑CCAGGCAGAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCa                          <  1:1413690/140‑1 (MQ=255)                                                                                                                     aaGGTAATTC‑CCAGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCa                          <  2:436296/139‑1 (MQ=37)                                                                                                                     aaGGTAAATT‑CCAGGCAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCa                          <  1:1066041/139‑1 (MQ=255)                                                                                                                          aaaTT‑CCAGGCAGAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTg                     <  2:445738/140‑1 (MQ=255)                                                                                                                          aaaTT‑CCAGGCAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTg                     <  1:1619201/140‑1 (MQ=255)                                                                                                                           aaTT‑CCAGGCAGAAAAAAA‑CCCCGCCGGTG‑‑GGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAg                  <  1:1162264/140‑1 (MQ=255)                                                                                                                                      aGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATa          >  2:1679157/1‑140 (MQ=255)                                                                                                                                        aaaaaaa‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGGTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTg                     >  2:909749/1‑127 (MQ=255)                                                                                                                                        aaaaaaa‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTg                     <  1:909749/127‑1 (MQ=255)                                                                                                                                        aaaaaaa‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAag        >  2:1076199/1‑140 (MQ=255)                                                                                                                                              a‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagcgcagc  <  1:764245/140‑1 (MQ=255)                                                                                                                                              |                                                                                                                                             AGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTT‑GGGGGTTACAGGTAAATT‑CCAGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGC  >  NC_000913/2001779‑2002057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑