Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	745,954	T→A	I7N (ATC→AAC)	nei →	endonuclease VIII

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	745,954	0	T	A	100.0%	204.8 / NA	59	I7N (ATC→AAC)	nei	endonuclease VIII
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (34/25);  total (34/25)

GACGGCGAGCACGCACTGGCGTTCGCCCGCCGACTACGCTCTGCATTTGCCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCATGCCTGAAGGCCCGGAGATCCGCCGTGCAGCGGATAACCTGGAGGCGGCGATCAAAGGCAAACCACTAACTGATGTCTGGTTTGCCTTCCCGCAGTTAAAACCTTATCAATCACAACTTATCGGTCAACACGTTACCCATGTGGAAACGCGTGGT  >  NC_000913/745822‑746090                                                                                                                                     |                                                                                                                                         gACGGCGAGCACGCACTGGCGTTCGCCCGCCGACTACGCTCTGCATTTGCCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCATGCCTGAAGGCCCGGAGAACCGCCGt                                                                                                                                   >  2:990924/1‑140 (MQ=255) gACGGCGAGCACGCACTGGCGTTCGCCCGCCGACTACGCTCTGCATTTGCCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCATGCCTGAAGGCCCGGAGAACCGCCGt                                                                                                                                   >  1:1516374/1‑140 (MQ=255)   cGGCGAGCACGCACTGGCGTTCGCCCGCCGACTACGCTCTGCATTTGCCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCATGCCTGAAGGCCCGGAGAACCGCCGTGc                                                                                                                                 >  2:1490548/1‑140 (MQ=255)    ggCGAGCACGCACTGGCGTTCGCCCGCCGACTACGCTCTGCATTTGCCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCATGCCTGAAGGCCCGGAGAACCGCCGTGCa                                                                                                                                <  1:1530201/140‑1 (MQ=255)         gcacgcacTGGCGTTCGCCCGCCGACTACGCTCTGCATTTGCCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCATGCCTGAAGGCCCGGAGAACCGCCGTGCAGCGGa                                                                                                                           <  1:990924/140‑1 (MQ=255)          cacgcacTGGCGTTCGCCCGCCGACTACGCTCTGCATTTGCCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCATGCCTGAAGGCCCGGAGAACCGCCGTGCAGCGGAt                                                                                                                          >  2:663147/1‑140 (MQ=255)                    cGTTCGCCCGCCGACTACGCTCTGCATTTGCCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCATGCCTGAAGGCCCGGAGAACCGCCGTGCa                                                                                                                                <  1:359717/124‑1 (MQ=255)                    cGTTCGCCCGCCGACTACGCTCTGCATTTGCCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCATGCCTGAAGGCCCGGAGAACCGCCGTGCa                                                                                                                                >  2:359717/1‑124 (MQ=255)                      ttCGCCCGCCGACTACGCTCTGCATTTGCCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCATGCCTGAAGGCCCGGAGAACCGCCGTGCAGCGGATAACCTGGAggcg                                                                                                              <  1:690854/140‑1 (MQ=255)                        cgcccgccGACTACGCTCTGCATTTGCCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCATGCCTGAAGGCCCGGAGAACCGCCGTGCAGCGGATAACCTGGAggcggc                                                                                                            >  2:1330021/1‑140 (MQ=255)                           ccgccgACTACGCTCTGCATTTGCCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCATGCCTGAAGGCCCGGAGAACCGCCGTGCAGCGGATAACCTGGAGGCGGCGAt                                                                                                         >  2:357483/1‑140 (MQ=255)                                  cTACGCTCTGCATTTGCCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCATGCCTGAAGGCCCGGAGAACCGCCGTGCAGCGGATAACCTGGAGGCGGCGATCAAAGGc                                                                                                  <  1:1518944/140‑1 (MQ=255)                                         ctGCATTTGCCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCATGCCTGAAGGCCCGGAGAACCGCCGTGCAGCGGATAACC‑GGAGGCGGCGATCAAAGGCAAACCACt                                                                                          <  1:378167/140‑1 (MQ=255)                                           gCATTTGCCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCATGCCTGAAGGCCCGGAGAACCGCCGTGCAGCGGATAACCTGGAGGCGGCGATCAAAGGCAAACCACTa                                                                                         >  2:1702298/1‑140 (MQ=255)                                               ttGCCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCATGCCTGAAGGCCCGGAGAACCGCCGTGCAGCGGATAACCTGGAGGCGGCGATCAAAGGCAAAcc                                                                                             >  1:1707575/1‑132 (MQ=255)                                               ttGCCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCATGCCTGAAGGCCCGGAGAACCGCCGTGCAGCGGATAACCTGGAGGCGGCGATCAAAGGCAAAcc                                                                                             <  2:1707575/132‑1 (MQ=255)                                                 gCCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCATGCCTGAAGGCCCGGAGAACCGCCGTGCAGCGGATAACCTGGAGGCGGCGATCAAAGGCAAACCACTAACTGAt                                                                                   >  1:1721174/1‑140 (MQ=255)                                                 gCCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCATGCCTGAAGGCCCGGAGAACCGCCGTGCAGCGGATAACCTGGAGGCGGCGATCAAAGGCAAACCACTAACTGAt                                                                                   >  1:1084718/1‑140 (MQ=255)                                                  ccGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCATGCCTGAAGGCCCGGAGAACCGCCGTGCAGCGGATAACCTGGAGGCGGCGATCAAAGGCAAACCACTAACTGATg                                                                                  >  1:438000/1‑140 (MQ=255)                                                      aaaGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCATGCCTGAAGGCCCGGAGAACCGCCGTGCAGCGGATAACCTGGAGGCGGCGATCAAAGGCAAACCACTAACTGATGTCTg                                                                              <  1:1490548/140‑1 (MQ=255)                                                      aaaGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCATGCCTGAAGGCCCGGAGAACCGCCGTGCAGCGGATAACCTGGAGGCGGCGATCAAAGGCAAACCACTAACTGATGTCTg                                                                              >  1:719140/1‑140 (MQ=255)                                                      aaaGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCATGCCTGAAGGCCCGGAGAACCGCCGTGCAGCGGATAACCTGGAGGCGGCGATCAAAGGCAAACCACTAACTGATGTCTg                                                                              >  1:1004580/1‑140 (MQ=255)                                                       aaGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCATGCCTGAAGGCCCGGAGAACCGCCGTGCAGCGGATAACCTGGAGGCGGCGATCAAAGGCAAACCACTAACTGATGTCTgg                                                                             <  2:438000/140‑1 (MQ=255)                                                                  tgtCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCATGCCTGAAGGCCCGGAGAACCGCCGTGCAGCGGATAACCTGGAGGCGGCGATCAAAGGCAAACCACTAACTGATGTCTGGTTTGCCTTccc                                                                  >  1:783224/1‑140 (MQ=255)                                                                   gtCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCATGCCTGAAGGCCCGGAGAACCGCCGTGCAGCGGATAACCTGGAGGCGGCGATCAAAGGCAAACCACTAACTGATGTCTGGTTTGCCTTCCCg                                                                 <  1:357483/140‑1 (MQ=255)                                                                   gtCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCATGCCTGAAGGCCCGGAGAACCGCCGTGCAGCGGATAACCTGGAGGCGGCGATCAAAGGCAAACCACTAACTGATGTCTGGTTTGCCTTCCCg                                                                 <  1:587445/140‑1 (MQ=255)                                                                   gtCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCATGCCTGAAGGCCCGGAGAACCGCCGTGCAGCGGATAACCTGGAGGCGGCGATCAAAGGCAAACCACTAACTGATGTCTGGTTTGCCTTCCCg                                                                 <  1:663147/140‑1 (MQ=255)                                                                    tCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCATGCCTGAAGGCCCGGAGAACCGCCGTGCAGCGGATAACCTGGAGGCGGCGATCAAAGGCAAACCACTAACTGATGTCTGGTTTGCCTTCCCGc                                                                >  1:1514578/1‑140 (MQ=255)                                                                    tCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCATGCCTGAAGGCCCGGAGAACCGCCGTGCAGCGGATAACCTGGAGGCGGCGATCAAAGGCAAACCACTAACTGATGTCTGGTTTGCCTTCCCGc                                                                >  2:1375298/1‑140 (MQ=255)                                                                    tCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCATGCCTGAAGGCCCGGAGAACCGCCGTGCAGCGGATAACCTGGAGGCGGCGATCAAAGGCAAACCACTAACTGATGTCTGGTTTGCCTTCCCGc                                                                >  1:458503/1‑140 (MQ=255)                                                                         gcaTAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCATGCCTGAAGGCCCGGAGAACCGCCGTGCAGCGGATAACCTGGAGGCGGCGATCAAAGGCAAACCACTAACTGATGTCTGGTTTGCCTTCCCGCAGTTa                                                           <  2:783224/140‑1 (MQ=255)                                                                           aTAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCATGCCTGAAGGCCCGGAGAACCGCCGTGCAGCGGATAACCTGGAGGCGGCGATCAAAGGCAAACCACTAACTGATGTCTGGTTTGCCTTCCCGCAGTTaaa                                                         >  1:1316287/1‑140 (MQ=255)                                                                                    aTTAATAAAGAATGAAAAAAGGATATCACCATGCCTGAAGGCCCGGAGAACCGCCGTGCAGCGGATAACCTGGAGGCGGCGATCAAAGGCAAACCACTAACTGATGTCTGGTTTGCCTTCCCGCAGTTAAAACCTTatca                                                >  2:593969/1‑140 (MQ=255)                                                                                    aTTAATAAAGAATGAAAAAAGGATATCACCATGCCTGAAGGCCCGGAGAACCGCCGTGCAGCGGATAACCTGGAGGCGGCGATCAAAGGCAAACCACTAACTGATGTCTGGTTTGCCTTCCCGCAGTTAAAACCTTatca                                                >  1:550746/1‑140 (MQ=255)                                                                                    aTTAATAAAGAATGAAAAAAGGATATCACCATGCCTGAAGGCCCGGAGAACCGCCGTGCAGCGGATAACCTGGAGGCGGCGATCAAAGGCAAACCACTAACTGATGTCTGGTTTGCCTTCCCGCAGTTAAAACCTTatca                                                >  1:30300/1‑140 (MQ=255)                                                                                    aTTAATAAAGAATGAAAAAAGGATATCACCATGCCTGAAGGCCCGGAGAACCGCCGTGCAGCGGATAACCTGGAGGCGGCGATCAAAGGCAAACCACTAACTGATGTCTGGTTTGCCTTCCCGCAGTTAAAACCTTatca                                                >  1:256012/1‑140 (MQ=255)                                                                                    aTTAATAAAGAATGAAAAAAGGATATCACCATGCCTGAAGGCCCGGAGAACCGCCGTGCAGCGGATAACCTGGAGGCGGCGATCAAAGGCAAACCACTAACTGATGTCTGGTTTGCCTTCCCGCAGTTAAAACCTTatca                                                >  2:1391637/1‑140 (MQ=255)                                                                                        ataaAGAATGAAAAAAGGATATCACCATGCCTGAAGGCCCGGAGAACCGCCGTGCAGCGGATAACCTGGAGGCGGCGATCAAAGGCAAACCACTAACTGATGTCTGGTTTGCCTTCCCGCAGTTAAAACCTTatcaatca                                            <  2:1084718/140‑1 (MQ=255)                                                                                         taaAGAATGAAAAAAGGATATCACCATGCCTGAAGGCCCGGAGAACCGCCGTGCAGCGGATAACCTGGAGGCGGCGATCAAAGGCAAACCACTAACTGATGTCTGGTTTGCCTTCCCGCAGTTAAAACCTTatcaat                                              >  2:181556/1‑137 (MQ=255)                                                                                         taaAGAATGAAAAAAGGATATCACCATGCCTGAAGGCCCGGAGAACCGCCGTGCAGCGGATAACCTGGAGGCGGCGATCAAAGGCAAACCACTAACTGATGTCTGGTTTGCCTTCCCGCAGTTAAAACCTTatcaat                                              <  1:181556/137‑1 (MQ=255)                                                                                                tGAAAAAAGGATATCACCATGCCTGAAGGCCCGGAGAACCGCCGTGCAGCGGATAACCTGGAGGCGGCGATCAAAGGCAAACCACTAACTGATGTCTGGTTTGCCTTCCCGCAGTTAAAACCTTATCAATCACAACTTAt                                    <  2:30300/140‑1 (MQ=255)                                                                                                tGAAAAAAGGATATCACCATGCCTGAAGGCCCGGAGAACCGCCGTGCAGCGGATAACCTGGAGGCGGCGATCAAAGGCAAACCACTAACTGATGTCTGGTTTGCCTTCCCGCAGTTAAAACCTTATCAATCACAACTTAt                                    <  2:1721174/140‑1 (MQ=255)                                                                                                tGAAAAAAGGATATCACCATGCCTGAAGGCCCGGAGAACCGCCGTGCAGCGGATAACCTGGAGGCGGCGATCAAAGGCAAACCACTAACTGATGTCTGGTTTGCCTTCCCGCAGTTAAAACCTTATCAATCACAACTTAt                                    <  1:694811/140‑1 (MQ=255)                                                                                                    aaaaGGATATCACCATGCCTGAAGGCCCGGAGAACCGCCGTGCAGCGGATAACCTGGAGGCGGCGATCAAAGGCAAACCACTAACTGATGTCTGGTTTGCCTTCCCGCAGTTAAAACCTTATCAATCACAACTTATCGGt                                <  2:1316287/140‑1 (MQ=255)                                                                                                        ggATATCACCATGCCTGAAGGCCCGGAGAACCGCCGTGCAGCGGATAACCTGGAGGCGGCGATCAAAGGCAAACCACTAACTGATGTCTGGTTTGCCTTCCCGCAGTTAAAACCTTATCAATCACAACTTATCGGTCAac                            >  2:1018225/1‑140 (MQ=255)                                                                                                        ggATATCACCATGCCTGAAGGCCCGGAGAACCGCCGTGCAGCGGATAACCTGGAGGCGGCGATCAAAGGCAAACCACTAACTGATGTCTGGTTTGCCTTCCCGCAGTTAAAACCTTATCAATCACAACTTATCGGTCAac                            >  2:907023/1‑140 (MQ=255)                                                                                                        ggATATCACCATGCCTGAAGGCCCGGAGAACCGCCGTGCAGCGGATAACCTGGAGGCGGCGATCAAAGGCAAACCACTAACTGATGTCTGGTTTGCCTTCCCGCAGTTAAAACCTTATCAATCACAACTTATCGGTCAac                            >  1:1697824/1‑140 (MQ=255)                                                                                                         gATATCACCATGCCTGAAGGCCCGGAGAACCGCCGTGCAGCGGATAACCTGGAGGCGGCGATCAAAGGCAAACCACTAACTGATGTCTGGTTTGCCTTCCCGCAGTTAAAACCTTATCAATCACAACTTATCGGTCAaca                           <  1:1018225/140‑1 (MQ=255)                                                                                                                 cATGCCTGAAGGCCCGGAGAACCGCCGTGCAGCGGATAACCTGGAGGCGGCGATCAAAGGCAAACCACTAACTGATGTCTGGTTTGCCTTCCCGCAGTTAAAACCTTATCAATCACAACTTATCGGTCAACACGTTAccc                   <  1:1375298/140‑1 (MQ=255)                                                                                                                    gCCTGAAGGCCCGGAGAACCGCCGTGCAGCGGATAACCTGGAGGCGGCGATCAAAGGCAAACCACTAACTGATGTCTGGTTTGCCTTCCCGCAGTTAAAACCTTATCAATCACAACTTATCGGTCAACACGTTACCCAtg                >  2:406964/1‑140 (MQ=255)                                                                                                                       tGAAGGCCCGGAGAACCGCCGTGCAGCGGATAACCTGGAGGCGGCGATCAAAGGCAAACCACTAACTGATGTCTGGTTTGCCTTCCCGCAGTTAAAACCTTATCAATCACAACTTATCGGTCAACACGTTACCCATGTgg             <  2:719140/140‑1 (MQ=255)                                                                                                                       tGAAGGCCCGGAGAACCGCCGTGCAGCGGATAACCTGGAGGCGGCGATCAAAGGCAAACCACTAACTGATGTCTGGTTTGCCTTCCCGCAGTTAAAACCTTATCAATCACAACTTATCGGTCAACACGTTACCCATGTgg             <  2:1004580/140‑1 (MQ=255)                                                                                                                          aGGCCCGGAGAACCGCCGTGCAGCGGATAACCTGGAGGCGGCGATCAAAGGCAAACCACTAACTGATGTCTGGTTTGCCTTCCCGCAGTTAAAACCTTATCAATCACAACTTATCGGTCAACACGTTACCCATGTGGaaa          >  1:669741/1‑140 (MQ=255)                                                                                                                              ccGGAGAACCGCCGTGCAGCGGATAACCTGGAGGCGGCGATCAAAGGCAAACCACTAACTGATGTCTGGTTTGCCTTCCCGCAGTTAAAACCTTATCAATCACAACTTATCGGTCAACACGTTACCCAtgtg              <  1:1629557/132‑1 (MQ=255)                                                                                                                              ccGGAGAACCGCCGTGCAGCGGATAACCTGGAGGCGGCGATCAAAGGCAAACCACTAACTGATGTCTGGTTTGCCTTCCCGCAGTTAAAACCTTATCAATCACAACTTATCGGTCAACACGTTACCCAtgtg              >  2:1629557/1‑132 (MQ=255)                                                                                                                              ccGGAGAACCGCCGTGCAGCGGATAACCTGGAGGCGGCGATCAAAGGCAAACCACTAACTGATGTCTGGTTTGCCTTCCCGCAGTTAAAACCTTATCAATCACAACTTATCGGTCAACACGTTACCCATGTGGAAAcgcg      >  2:495990/1‑140 (MQ=255)                                                                                                                               cGGAGAACCGCCGTGCAGCGGATAACCTGGAGGCGGCGATCAAAGGCAAACCACTAACTGATGTCTGGTTTGCCTTCCCGCAGTTAAAACCTTATCAATCACAACTTATCGGTCAACACGTTACCCATGTGGAAACGCGt     >  1:1098552/1‑140 (MQ=255)                                                                                                                                ggAGAACCGCCGTGCAGCGGATAACCTGGAGGCGGCGATCAAAGGCAAACCACTAACTGATGTCTGGTTTGCCTTCCCGCAGTTAAAACCTTATCAATCACAACTTATCGGTCAACACGTTACCCATGTGGAAACGCGTg    >  1:1658959/1‑140 (MQ=255)                                                                                                                                  agaACCGCCGTGCAGCGGATAACCTGGAGGCGGCGATCAAAGGCAAACCACTAACTGATGTCTGGTTTGCCTTCCCGCAGTTAAAACCTTATCAATCACAACTTATCGGTCAACACGTTACCCATGTGGAAACGCGTGGt  <  1:1702298/140‑1 (MQ=255)                                                                                                                                  agaACCGCCGTGCAGCGGATAACCTGGAGGCGGCGATCAAAGGCAAACCACTAACTGATGTCTGGTTTGCCTTCCCGCAGTTAAAACCTTATCAATCACAACTTATCGGTCAACACGTTACCCATGTGGAAACGCGTGGt  <  2:256012/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                         GACGGCGAGCACGCACTGGCGTTCGCCCGCCGACTACGCTCTGCATTTGCCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCATGCCTGAAGGCCCGGAGATCCGCCGTGCAGCGGATAACCTGGAGGCGGCGATCAAAGGCAAACCACTAACTGATGTCTGGTTTGCCTTCCCGCAGTTAAAACCTTATCAATCACAACTTATCGGTCAACACGTTACCCATGTGGAAACGCGTGGT  >  NC_000913/745822‑746090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑