| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,825,061 | 0 | G | A | 23.8% | 89.7 / 11.0 | 42 | intergenic (‑124/+79) | ves/spy | HutD family protein Ves/ATP‑independent periplasmic chaperone |
| Reads supporting (aligned to +/- strand): ref base G (28/4); new base A (0/10); total (28/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.80e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGC‑AACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCA > NC_000913/1824926‑1825200 | aaaGTATCCCATACTCGCTCCCGGCCTTTTTTTTTCTTTTTTATCGTTCAGGTAACGGTGGATGTTTATGGGC‑ACCTGGGGTGATTTACGTTAGTGGTGATCAGTTTTTTTTTAAAA‑CTTTTT‑GAAAAGAaaaaaaaaaa < 2:185567/140‑1 (MQ=255) tCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGC‑AACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTT‑GAGAAGAGAAAAGAAAACCGCCGa > 1:1448250/1‑140 (MQ=255) cATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGC‑AACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATc > 1:37649/1‑140 (MQ=255) tCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGC‑AACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTc > 1:1121418/1‑140 (MQ=255) tCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGC‑AACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTc > 1:332964/1‑140 (MQ=255) tCGCCCCCGGCCCTTTTTTTTTTTTTTTTTCGTTCAGGTACCGGTGGTTGGTTACCGAC‑AACTGGGGTGATTTACGTTAGTGGTAATCGGGTTTTTTTTACAA‑TTTTTT‑GAGAAGAAAAAAAAAAACCGCCGATCCTGTc < 2:593245/140‑1 (MQ=255) tCGCCCCCGGCCCTTTTTTTTATTTTTTATCGTTCAGGTACCGGTGGATGTTTATCGGC‑AACTGGGGTGATTTACGTTGGTGGTGATCAGGTTTTTTTTACAA‑TTGTTT‑GAAAAGAAAAAAAAAAACCGCCGATCCTGTc < 2:1194565/140‑1 (MQ=255) gCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGC‑AACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCa > 1:831205/1‑140 (MQ=255) gCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGC‑AACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCa > 1:82005/1‑140 (MQ=255) gCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGC‑AACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCa > 1:727011/1‑140 (MQ=255) cTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGC‑AACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCAc > 1:1194565/1‑140 (MQ=255) cGGCCCTTTTTTTTCATTGTTATCGTTCAGGTACCGGTGGATGTTTATCGGC‑AACTCGGGTTATTTAGTTTAGTGGTGATCGGGTTTTTTTTACAA‑CTTTTT‑GAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCa < 2:1448250/140‑1 (MQ=255) cGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGC‑AACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACt > 2:78941/1‑140 (MQ=255) ttCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGC‑AACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGc > 1:935654/1‑140 (MQ=255) ttttttCTTTGTTATCTTTCAGGTACCGGTGGTTGTTTATCGGC‑ACCTGGGGTTATTTACTTTAGTGGTGATCAGGTTTTTTTTACAA‑CTTTTG‑GAGAAGAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCa < 2:935654/138‑1 (MQ=255) ttttttCATTTTTATCTTACAGATACCGGTGGTGGTTTACGGGC‑AACTGGGGTGATTTACGTTAGGGGTAATCAGG‑TTTTTTTAAAA‑TTTTTTGGAAAAGAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCa < 2:1121418/138‑1 (MQ=255) cTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGC‑AACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCaa > 1:1626574/1‑140 (MQ=255) aTCGTTCAGGTAACCGTGGATGGTTATCGGC‑AACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACaa > 1:387157/1‑140 (MQ=255) ttCAGGTAACCGTGGATGGTTATCGGC‑AACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGAcc > 1:1174938/1‑140 (MQ=255) ggggTACCCGTGGTTGTTTA‑CGGCAAACTGGGGTGTTTTCCGTTAGGGGTGATCAGGTTTTTTTAAAAT‑TTTTTT‑GAGAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcgg < 2:332964/138‑1 (MQ=255) cAGGTAACGGTGGATGTTTTTGGGC‑AACTGGGGTGATTTAGGTTAGTGGTAACCAGTTTTTTTTTACAA‑CTGTTT‑AAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcgg < 2:153834/140‑1 (MQ=255) cAGGTAACCGTGGATGGTTATCGGC‑AACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcgg > 2:772734/1‑140 (MQ=255) ggTAACGGTGGATGTTTATCGGC‑AACTGGGGTAATTTACGTAGGTGGTGATCAGTTTTTTTTTACAT‑CTTTTG‑GAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggcg < 2:387157/140‑1 (MQ=255) ggTAACCGTGGATGGTTATCGGC‑AACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggcg > 2:183126/1‑140 (MQ=255) cGTGGATGGTTATCGGC‑AACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTa > 2:655258/1‑140 (MQ=255) aTGGTTATCGGC‑AACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGttt > 1:765794/1‑140 (MQ=255) aTGGTTATCGGC‑AACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGttt > 2:1278595/1‑140 (MQ=255) gTTATCGGC‑AACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGtttttt > 1:1445004/1‑140 (MQ=255) ttATCGGC‑AACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTg > 2:880042/1‑140 (MQ=255) tATCGGC‑AACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTgg > 1:1692803/1‑140 (MQ=255) cGGC‑ATCTGGGGTGTTTTCCGTTGGTGGTGATCGGGTTTTTTTTACAT‑TTGTTT‑GAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTg < 2:765794/140‑1 (MQ=255) aaCTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAg > 1:939533/1‑140 (MQ=255) ttAGTGGTGATCAGTTTTTTTTTACAA‑CTGTTT‑GAGAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCa < 1:1538388/140‑1 (MQ=255) tggtgATCAGGCTTTTTTTACAA‑CTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCa > 2:68445/1‑140 (MQ=255) tggtgATCAGGCTTTTTTTACAA‑CTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCa > 1:966916/1‑140 (MQ=255) gATCAGGCTTTTTTTACAA‑CTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCAtttt > 1:130560/1‑140 (MQ=255) gttttttttCAACCTGTTA‑GAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCttt < 2:1626574/139‑1 (MQ=255) ttttaACAA‑CTGTTA‑GAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGcc < 2:939533/140‑1 (MQ=255) a‑CTTTTG‑GAGAAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACg < 1:3856/140‑1 (MQ=255) gTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACCGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGttt > 1:1034864/1‑139 (MQ=255) t‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGt > 2:1114144/1‑140 (MQ=255) aagagaaAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGa < 2:1692803/140‑1 (MQ=255) aagagaaAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGa < 1:880042/140‑1 (MQ=255) aaaaaaaaaaaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGa < 2:1445004/135‑1 (MQ=255) agagaaAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGAc < 1:1114144/140‑1 (MQ=255) gagaaAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACg > 2:1477976/1‑140 (MQ=255) gaaaagaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCt > 1:564052/1‑140 (MQ=255) gaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCa < 1:78941/140‑1 (MQ=255) | AAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGC‑AACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCA > NC_000913/1824926‑1825200 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |