Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,907,896	A→T	intergenic (‑305/+365)	yobF ← / ← yebO	DUF2527 domain‑containing protein YobF/uncharacterized protein YebO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,907,896	0	A	T	100.0%	342.0 / NA	94	intergenic (‑305/+365)	yobF/yebO	DUF2527 domain‑containing protein YobF/uncharacterized protein YebO
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (60/34);  total (60/34)

TGCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCAAAGGGATTGTAAATTTAAAATAAGAAAAATTGATGAATGAGCAAAAAAATCAA  >  NC_000913/1907763‑1908020                                                                                                                                      |                                                                                                                             tGCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTtata                                                                                                                        >  1:1441644/1‑140 (MQ=255) tGCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTtata                                                                                                                        >  1:1478886/1‑140 (MQ=255)  gCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTatat                                                                                                                       >  2:586271/1‑140 (MQ=255)       ttCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTtat                                                                                                                         >  2:289940/1‑133 (MQ=255)       ttCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTtat                                                                                                                         <  1:289940/133‑1 (MQ=255)        tCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACttt                                                                                                                 >  1:1088842/1‑140 (MQ=255)            gCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTtct                                                                                                             >  2:1022409/1‑140 (MQ=255)            gCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTtct                                                                                                             >  2:505041/1‑140 (MQ=255)               gATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCAt                                                                                                          <  1:893125/140‑1 (MQ=255)                aTACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATa                                                                                                         <  2:1088842/140‑1 (MQ=255)                aTACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATa                                                                                                         >  1:1115707/1‑140 (MQ=255)                aTACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATa                                                                                                         <  1:1572728/140‑1 (MQ=255)                 tACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATaa                                                                                                        <  2:1115707/140‑1 (MQ=255)                 tACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATaa                                                                                                        <  2:679910/140‑1 (MQ=255)                 tACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATaa                                                                                                        <  1:140876/140‑1 (MQ=255)                       aaCTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAAcagt                                                                                                  <  1:1354588/140‑1 (MQ=255)                       aaCTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAAcagt                                                                                                  <  2:1240887/140‑1 (MQ=255)                       aaCTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAAcagt                                                                                                  <  2:991230/140‑1 (MQ=255)                       aaCTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAAcagt                                                                                                  <  1:505041/140‑1 (MQ=255)                            gCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGtt                                                                                             >  1:1038780/1‑140 (MQ=255)                            gCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGtt                                                                                             >  2:1599186/1‑140 (MQ=255)                            gCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGtt                                                                                             >  2:444277/1‑140 (MQ=255)                            gCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGtt                                                                                             >  2:17294/1‑140 (MQ=255)                            gCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGtt                                                                                             >  1:1534041/1‑140 (MQ=255)                             cGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTa                                                                                            <  1:1620854/140‑1 (MQ=255)                               tATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAAc                                                                                          >  2:741690/1‑140 (MQ=255)                                      tAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTatt                                                                                   >  2:1333211/1‑140 (MQ=255)                                      tAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTatt                                                                                   >  1:9564/1‑140 (MQ=255)                                         cATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCGCTTTTTCTCATAAAACAGTCAGTTAACGGCTattaat                                                                                <  2:1029406/140‑1 (MQ=255)                                         cATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTattaat                                                                                <  2:377314/140‑1 (MQ=255)                                         cATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTattaat                                                                                <  1:741690/140‑1 (MQ=255)                                          aTAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTattaatt                                                                               <  2:1478886/140‑1 (MQ=255)                                           tAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTattaatta                                                                              >  2:795757/1‑140 (MQ=255)                                           tAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTattaatta                                                                              >  1:1652125/1‑140 (MQ=255)                                             gTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACgg                                                                                        >  1:666172/1‑128 (MQ=255)                                             gTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACgg                                                                                        <  2:666172/128‑1 (MQ=255)                                              ttCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACgg                                                                                        >  2:1473672/1‑127 (MQ=255)                                              ttCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACgg                                                                                        <  1:1473672/127‑1 (MQ=255)                                              ttCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTAccc                                                                           >  2:222419/1‑140 (MQ=255)                                               tCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCt                                                                          <  1:794970/140‑1 (MQ=255)                                                cAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTa                                                                         >  2:86597/1‑140 (MQ=255)                                                   tAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCt                                                                          <  2:408300/136‑1 (MQ=255)                                                   tAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCt                                                                          >  1:408300/1‑136 (MQ=255)                                                   tAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAag                                                                      >  2:34474/1‑140 (MQ=255)                                                        tCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAg                                                                 >  1:1035204/1‑140 (MQ=255)                                                            ttgttCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCaa                                                             <  1:444277/140‑1 (MQ=255)                                                            ttgttCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCaa                                                             <  2:1534041/140‑1 (MQ=255)                                                                 ccAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATcccc                                                        >  1:1347207/1‑140 (MQ=255)                                                                 ccAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATcccc                                                        <  2:1441644/140‑1 (MQ=255)                                                                   aGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCaa                                                      <  2:793938/140‑1 (MQ=255)                                                                   aGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCaa                                                      <  1:222419/140‑1 (MQ=255)                                                                   aGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCaa                                                      <  1:475061/140‑1 (MQ=255)                                                                    ggCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCaaa                                                     >  2:1246621/1‑140 (MQ=255)                                                                         gTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCAAAGGGAt                                                >  1:1022492/1‑140 (MQ=255)                                                                          tGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCaa                                                      <  1:1137606/133‑1 (MQ=255)                                                                          tGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCaa                                                      >  2:1137606/1‑133 (MQ=255)                                                                          tGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCAAAGGGAtt                                               >  2:840196/1‑140 (MQ=255)                                                                              agaAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCAAAGGGATTGTaa                                           >  2:543419/1‑140 (MQ=255)                                                                                aaGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCAAAGGGATTGTAAAt                                         >  2:341918/1‑140 (MQ=255)                                                                                aaGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCAAAGGGATTGTAAAt                                         >  2:1563729/1‑140 (MQ=255)                                                                                    tattaCATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCATAAAGAGAAGTCAATCCCCAAAGGGATTGTAAATTTaa                                     >  1:454751/1‑140 (MQ=255)                                                                                      ttaCATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCAAAGGGATTGTAAATTTaaaa                                   >  2:626684/1‑140 (MQ=255)                                                                                        aCATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCAAAGGGATTGTAAATTTAAAATa                                 >  2:318419/1‑140 (MQ=255)                                                                                            aGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCAAAGGGATTGTAAATTTAAAATAAg                               <  2:712203/138‑1 (MQ=255)                                                                                            aGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCAAAGGGATTGTAAATTTAAAATAAg                               <  1:537228/138‑1 (MQ=255)                                                                                            aGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCAAAGGGATTGTAAATTTAAAATAAg                               >  2:537228/1‑138 (MQ=255)                                                                                            aGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCAAAGGGATTGTAAATTTAAAATAAg                               >  1:712203/1‑138 (MQ=255)                                                                                            aGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCAAAGGGATTGTAAATTTAAAATAAGaa                             <  2:539050/140‑1 (MQ=255)                                                                                             gtgcgtgcAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCAAAGGGATTGTAAATTTAAAATAAGaaa                            >  2:1146536/1‑140 (MQ=255)                                                                                             gtgcgtgcAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCAAAGGGATTGTAAATTTAAAATAAGaaa                            >  1:1232087/1‑140 (MQ=255)                                                                                              tgcgtgcAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCAAAGGGATTGTAAATTTAAAATAAGaaaa                           <  2:1232087/140‑1 (MQ=255)                                                                                              tgcgtgcAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCAAAGGGATTGTAAATTTAAAATAAGaaaa                           >  2:406020/1‑140 (MQ=255)                                                                                              tgcgtgcAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCAAAGGGATTGTAAATTTAAAATAAGaaaa                           >  2:1112097/1‑140 (MQ=255)                                                                                              tgcgtgcAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCAAAGGGATTGTAAATTTAAAATAAGaaaa                           >  1:231182/1‑140 (MQ=255)                                                                                              tgcgtgcAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCAAAGGGATTGTAAATTTAAAATAAGaaaa                           >  2:1204616/1‑140 (MQ=255)                                                                                              tgcgtgcAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCAAAGGGATTGTAAATTTAAAATAAGaaaa                           >  2:1406092/1‑140 (MQ=255)                                                                                              tgcgtgcAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCAAAGGGATTGTAAATTTAAAATAAGaaaa                           >  2:1601741/1‑140 (MQ=255)                                                                                              tgcgtgcAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCAAAGGGATTGTAAATTTAAAATAAGaaaa                           >  1:1592591/1‑140 (MQ=255)                                                                                              tgcgtgcAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCAAAGGGATTGTAAATTTAAAATAAGaaaa                           >  1:557134/1‑140 (MQ=255)                                                                                              tgcgtgcAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCAAAGGGATTGTAAATTTAAAATAAGaaaa                           >  2:1514183/1‑140 (MQ=255)                                                                                                cgtgcAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCAAAGGGATTGTAAATTTAAAATAAGAAAAAt                         >  1:239487/1‑140 (MQ=255)                                                                                                cgtgcAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCAAAGGGATTGTAAATTTAAAATAAGAAAAAt                         >  2:265143/1‑140 (MQ=255)                                                                                                cgtgcAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCAAAGGGATTGTAAATTTAAAATAAGAAAAAt                         >  1:1617645/1‑140 (MQ=255)                                                                                                  tgcAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCAAAGGGATTGTAAATTTAAAATAAGAAAAATtg                       >  2:587959/1‑140 (MQ=255)                                                                                                  tgcAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCAAAGGGATTGTAAATTTAAAATAAGAAAAATtg                       >  1:658754/1‑140 (MQ=255)                                                                                                   gcAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCAAAGGGATTGTAAATTTAAAATAAGAAAAATtga                      >  2:780508/1‑140 (MQ=255)                                                                                                   gcAATATCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCAAAGGGATTGTAAATTTAAAATAAGAAAAATtga                      >  1:473217/1‑140 (MQ=255)                                                                                                        atCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCAAAGGGATTGTAAATTTAAAATAAGAAAAATTGatgaat                 >  2:1344383/1‑140 (MQ=255)                                                                                                        atCACATTTTTTGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCAAAGGGATTGTAAATTTAAAATAAGAAAAATTGatgaat                 >  2:1407273/1‑140 (MQ=255)                                                                                                        atCACATTTTTTGCTATGCAATGAATAAAATGGTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCAAAGGGATTGTAAATTTAAAATAAGAAAAATTGatgaat                 >  2:311727/1‑140 (MQ=255)                                                                                                                   tGCTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCAAAGGGATTGTAAATTTAAAATAAGAAAAATTGATGAATGAGCaaaaaaa      >  2:63774/1‑140 (MQ=255)                                                                                                                     cTATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCAAAGGGATTGTAAATTTAAAATAAGAAAAATTGATGAATGAGCAAAAAAATc    <  2:1042433/140‑1 (MQ=255)                                                                                                                      tATGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCAAAGGGATTGTAAATTTAAAATAAGAAAAATTGATGAATGAGCAAAAAAATCa   <  1:587959/140‑1 (MQ=255)                                                                                                                       aTGCAATGAATAAAATGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCAAAGGGATTGTAAATTTAAAATAAGAAAAATTGATGAATGAGCAAAAAAATCaa  <  2:9564/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                             TGCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAATCCCCAAAGGGATTGTAAATTTAAAATAAGAAAAATTGATGAATGAGCAAAAAAATCAA  >  NC_000913/1907763‑1908020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑