Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,370,371	Δ1 bp	coding (666/690 nt)	nanE ←	putative N‑acetylmannosamine‑6‑phosphate epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,370,371	0	T	.	100.0%	194.7 / NA	46	coding (666/690 nt)	nanE	putative N‑acetylmannosamine‑6‑phosphate epimerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (23/23);  total (23/23)

TGGTGTCTGGCTGGCTGGCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGCAATCACCCGACATC  >  NC_000913/3370237‑3370504                                                                                                                                       |                                                                                                                                      tGGTGTCTGGCTGGCTGGCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGC‑Gtgttg                                                                                                                                 >  2:332580/1‑140 (MQ=255)     gtCTGGCTGGCTGGCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGC‑GTGTTGTAcc                                                                                                                             >  2:528328/1‑140 (MQ=255)     gtCGGGCTGGCGGGCGTAGGAAGTTCACGACGATCGGGGATCTGCCCGTCAGCGCCAATCAGGGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGC‑GTGTTGTAcc                                                                                                                             <  2:52116/140‑1 (MQ=255)      tCTGGCTGGCTGGCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGC‑GTGTTGTACCa                                                                                                                            >  2:634033/1‑140 (MQ=255)          gctggctggcGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGC‑GTGTTGTACCACTGa                                                                                                                        >  1:557488/1‑140 (MQ=255)               cgggcGTGGAAAGTTCACGACGTNCNGGGATCTCCCCGCCAGCCCAAATCGGCGGGGCGGAAAGTTTATTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGC‑GTGTTGTACCACTGACAAAt                                                                                                                   <  2:139384/138‑1 (MQ=255)                    gTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGC‑GTGTTGTACCACTGACAAATGTGCt                                                                                                              >  1:770042/1‑140 (MQ=255)                            ttCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGC‑GTGTTGTACCACTGACaaa                                                                                                                    >  1:1031772/1‑126 (MQ=255)                            ttCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGC‑GTGTTGTACCACTGACaaa                                                                                                                    <  2:1031772/126‑1 (MQ=255)                            ttCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGC‑GTGTTGTACCACTGACAAATGTGCTCAAGAcgc                                                                                                      >  1:561856/1‑140 (MQ=255)                             tCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGC‑GTGTTGTACCACTGACAAATGTGCTCAAGAcgcg                                                                                                     >  1:529236/1‑140 (MQ=255)                              cACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGC‑GTGTTGTACCACTGACAAATGTGCTCAAGACGCGt                                                                                                    <  1:1019067/140‑1 (MQ=255)                                      tCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGC‑GTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAg                                                                                            >  1:24775/1‑140 (MQ=255)                                         cgGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGC‑GTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAac                                                                                         >  1:397582/1‑140 (MQ=255)                                               tGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGC‑GTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAaccgaccg                                                                                   >  2:202743/1‑140 (MQ=255)                                                   cGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGC‑GTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCAc                                                                               <  1:528328/140‑1 (MQ=255)                                                      cAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGC‑GTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGc                                                                            >  2:1053598/1‑140 (MQ=255)                                                      cAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGC‑GTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGc                                                                            >  1:292852/1‑140 (MQ=255)                                                      cAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGC‑GTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGc                                                                            >  1:598184/1‑140 (MQ=255)                                                        gcgcCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGC‑GTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAacc                                                                                        >  2:1079046/1‑126 (MQ=255)                                                        gcgcCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGC‑GTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAacc                                                                                        <  1:1079046/126‑1 (MQ=255)                                                        gcgcCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGC‑GTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGccc                                                                          >  1:267143/1‑140 (MQ=255)                                                           ccAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGC‑GTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCAcg                                                                       <  1:648767/140‑1 (MQ=255)                                                           ccAACCGGCGCGGCGGCAATTTTAGTCCCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGC‑GTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCAcg                                                                       <  2:557488/140‑1 (MQ=255)                                                                        cggcAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGC‑GTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGcgcat                                                          >  2:758771/1‑140 (MQ=255)                                                                          gcAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGC‑GTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGcgcatcg                                                        <  2:623991/140‑1 (MQ=255)                                                                               tttATTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGC‑GTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATcc                                                   <  2:1010629/140‑1 (MQ=255)                                                                                    tACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGC‑GTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCcgccgc                                              <  1:462579/140‑1 (MQ=255)                                                                                         ccgATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGC‑GTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAg                                         >  2:277925/1‑140 (MQ=255)                                                                                            atatCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGC‑GTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAg                                      <  2:900103/140‑1 (MQ=255)                                                                                               tCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGC‑GTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCg                                   <  2:292852/140‑1 (MQ=255)                                                                                               tCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGC‑GTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCg                                   <  1:394231/140‑1 (MQ=255)                                                                                                       cAGTGTGGTCATAGCACCGCCTTTTTCATCGC‑GTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTaa                           <  1:758771/140‑1 (MQ=255)                                                                                                       cAGTGTGGTCATAGCACCGCCTTTTTCATCGC‑GTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTaa                           <  1:332580/140‑1 (MQ=255)                                                                                                              gTCATAGCACCGCCTTTTTCATCGC‑GTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCtt                    <  1:537538/140‑1 (MQ=255)                                                                                                              gTCATAGCACCGCCTTTTTCATCGC‑GTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCtt                    <  1:477612/140‑1 (MQ=255)                                                                                                                  tAGCACCGCCTTTTTCATCGC‑GTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGc                <  2:24775/140‑1 (MQ=255)                                                                                                                    gCACCGCCTTTTTCATCGC‑GTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGc                <  1:895410/138‑1 (MQ=255)                                                                                                                    gCACCGCCTTTTTCATCGC‑GTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGc                >  2:895410/1‑138 (MQ=255)                                                                                                                    gCACCGCCTTTTTCATCGC‑GTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGCaa              >  1:143047/1‑140 (MQ=255)                                                                                                                      aCCGCCTTTTTCATCGC‑GTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACg                         >  1:1188180/1‑127 (MQ=255)                                                                                                                      aCCGCCTTTTTCATCGC‑GTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACg                         <  2:1188180/127‑1 (MQ=255)                                                                                                                      aCCGCCTTTTTCATCGC‑GTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGCAATc            >  1:545414/1‑140 (MQ=255)                                                                                                                      aCCGCCTTTTTCATCGC‑GTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGCAATc            <  2:598184/140‑1 (MQ=255)                                                                                                                        cGCCTTTTTCATCGC‑GTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGCAATCAc          >  2:670623/1‑140 (MQ=255)                                                                                                                          ccTTTTTCATCGC‑GTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGCAATCAccc        >  1:647654/1‑140 (MQ=255)                                                                                                                                tCATCGC‑GTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGCAATCACCCGACATc  <  1:1053598/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                      TGGTGTCTGGCTGGCTGGCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGCAATCACCCGACATC  >  NC_000913/3370237‑3370504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑