| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 646,747 | 0 | T | G | 26.2% | 81.0 / 15.9 | 42 | T255P (ACC→CCC) | citG | triphosphoribosyl‑dephospho‑CoA synthase |
| Reads supporting (aligned to +/- strand): ref base T (0/31); new base G (11/0); total (11/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.34e-10 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAA‑CGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGC > NC_000913/646617‑646874 | aaGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGcc < 1:923670/140‑1 (MQ=255) cTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTGGGATTGCCCCCt > 2:595066/1‑140 (MQ=255) agacagaatTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCttt < 2:911091/134‑1 (MQ=255) gacagaatTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCtttt < 1:1138071/135‑1 (MQ=255) tAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTCGGATGCCCCCCTTTTTCtataa > 2:464841/1‑140 (MQ=255) aTCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGt < 1:19574/140‑1 (MQ=255) tgCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTgcg < 1:744793/140‑1 (MQ=255) tgCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTTGGATGCCCCCCTTTTTTTATAATGtttttg > 2:604898/1‑137 (MQ=255) tgCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTCGGATGGCCCCCTTTTTTAATAAAGTTTtcg > 2:748016/1‑140 (MQ=255) tgCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTCGGATGCCCCCCTTTTTCCATAATGTCTgtt > 2:1138071/1‑138 (MQ=255) tgCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTCGGAAGCCCCCCTTTTTTAATAATAtttttg > 1:356978/1‑137 (MQ=255) tgCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTCGAATGCCCCCCTTTTTCAATAATGTTTtcg > 1:713644/1‑140 (MQ=255) gCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTTGGATGCCCCCCTTTTTCAATAATGttttccc > 1:56626/1‑136 (MQ=255) cTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTCGAATGCCCCCCTTTTTCAATAATGTTTGCGcc > 1:64569/1‑140 (MQ=255) tAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCt < 2:1253967/140‑1 (MQ=255) aaaaaCCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTc < 1:604898/140‑1 (MQ=255) cAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTCGAATGCCCCCCTTTTTTAATAATTTTTTGGCCTCCGGCTg > 2:744793/1‑140 (MQ=255) gTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAgg < 2:1116419/140‑1 (MQ=255) gTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAgg < 1:346971/140‑1 (MQ=255) gTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAgg < 1:748016/140‑1 (MQ=255) aGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGcc < 1:768772/140‑1 (MQ=255) cAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTCGAATACCCCCCTTTTTTAATAATATTTTCGCCTCCCGCTGTAGCCCGCCCACGcccccc > 2:1193200/1‑140 (MQ=255) cACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGGTGGATGTCCCCCTTTTTCCATAATTTTTTTGGCTCGCGCCGGAGGCAGCGCCGGccccccccc > 2:102706/1‑137 (MQ=255) cTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTCGAATGCCCCCCTTTTTCAATAATGTTTTCGCCTCGCGCTGTAGCCAGCGCCGGccccccccccc > 1:1032805/1‑135 (MQ=255) ccgccTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTCGGATTCCCCCCTTTTTTAATAATGTTTTCGCCTCCCGCTGTAGCCAGCGCAGGGCCCCCCCGCcccc > 2:469533/1‑140 (MQ=255) ccgccTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTCGAAAGCCCCCCTTTTTCAATAATGTTTTCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCCCCCCAcc > 1:494058/1‑140 (MQ=255) ccTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACcgcg < 2:35850/140‑1 (MQ=255) ttcgATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAA‑CGTTGGTAt < 1:595066/140‑1 (MQ=255) ttcgATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTGGGATGCCCCCCTTTTTCAATAAAATTTTTGCCCCCCGCCGTAGCCAGCGCAGGCCCCCCCCCCCCCCCCGCGATGCAA‑CGTTGGTAt > 2:87443/1‑140 (MQ=255) tACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTTGAATGCCCCCCTTTTTCAATAATTTTTTTGCCCCCCGCCGTAGGCAGCGCCGGCCCCCCCCCCCCCCCCGCGGTGCAA‑CGTTGGGTTCGccc > 2:332022/1‑139 (MQ=255) cTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAA‑CGTTGGTATCGCCGTTGa < 2:920318/140‑1 (MQ=255) cTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAA‑CGTTGGTATCGCCGTTGa < 2:356978/140‑1 (MQ=255) tGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAA‑CGTTGGTATCGCCGTTGATCGcc < 1:1043437/140‑1 (MQ=255) gTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAA‑CGTTGGTATCGCCGTTGATCGCCa < 2:91463/140‑1 (MQ=255) gTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAA‑CGTTGGTATCGCCGTTGATCGCCa < 1:332022/140‑1 (MQ=255) gTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTTGGATGCCCCCCTTTTTTTATAATGTTTTCGCCCCGCCCCGTAGCCAGCGCAGGGCCCCCCCCCCCCCCCCCGGTGCCA‑CGTTGGTGTTGCCGTTGGTCGccc > 2:137861/1‑139 (MQ=255) gTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTTGAATGCCCCCCTTTTTCAATAATGTTTTCGCCCCGCGCCGTAGCCAGCGCAGGCCCCCCCCCCCCCCCCGCGAGGCAA‑CGCTGGTATCGCCGGTGGTccccc > 2:395084/1‑139 (MQ=255) gTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTTGAATGCCCCCCTTTTTCAATAAAGTTTTTGCCTCCCCCTGTAGCCAGCGCCGGCCCCCCCCCCCCCCCCCCGCGGGAACCGTTGG‑ATCGCCGCTGATCGccc > 2:669802/1‑139 (MQ=255) gTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTCGCATGCCCCCCTTTTTCAATAATGTTTTCGCCCCGCGCCGTAGCCAGCGCCGGCCCCCCCCCCCCCCCCCCGGTGCAA‑CGGTGGTATCGCCCGTGGTCGccc > 2:1043437/1‑139 (MQ=255) tCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAA‑CGTTGGTATCGCCGTTGATCGCCAt < 2:1197711/140‑1 (MQ=255) tCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAA‑CGTTGGTATCGCCGTTGATCGCCAt < 1:102706/140‑1 (MQ=255) gacagcTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAA‑CGTTGGTATCGCCGTTGATCGCCAt < 1:542083/135‑1 (MQ=255) aaCTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAA‑CGTTGGTATCGCCGTTGATCGCCATCAg < 2:781973/140‑1 (MQ=255) aCTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAA‑CGTTGGTATCGCCGTTGATCGCCATCAGt < 2:64569/140‑1 (MQ=255) aCTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAA‑CGTTGGTATCGCCGTTGATCGCCATCAGt < 1:981790/140‑1 (MQ=255) aCTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAA‑CGTTGGTATCGCCGTTGATCGCCATCAGt < 1:137861/140‑1 (MQ=255) ccGGAGATAATCGAGATCGGC‑GGGGGGTTGGATGGCCCCCTTTTTTAATAATATTTTCGCCCCCCGCTGTAGCCCGCGCAGGCCCCCCCCCCCCCCCCCCGCGGCCA‑AGGTGGTATCGCCCGTGATCGCCACCAGTAggg > 2:128681/1‑139 (MQ=255) ccGGAGATAATCGAGATCGGC‑GGGGGGTCGGATGGCCCCCTTTTTTAATAATTTTTTCGCCCCGCGCTGTAGCCAGCGCAGGCCCCCCCCCCCCCCCCGCGATGCCA‑CGTTGGTATCGCCGGTGATTGCCATCaggaggg > 2:1174097/1‑139 (MQ=255) gagaTAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAA‑CGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCa < 1:1043758/140‑1 (MQ=255) gagaTAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCACCACCGCGCGATGCAA‑CGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCa < 1:930051/140‑1 (MQ=255) aaTCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAA‑CGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTa < 1:270975/140‑1 (MQ=255) aaTCGAGATCGGC‑GGGGGGTCGGATGCCCCCCTTTTTCTATAATGTTTTCGCCTCCCGCCGTAGCCAGCGCCGGCCCCCCCCCCCCCCCCGCCGTGCCA‑CG‑GGGTATCGCCCGTGATCGCCCTCAGTAGGAGGaaaggaa > 2:137710/1‑135 (MQ=255) aaTCGAGATCGGC‑GGGGGGTCGGATGCCCCCCTTTTTCAATAATTTTTTCGCCTCGCGCCGTAGCCAGCGCAGGCCCCCCCCCCCCCCCCCCGCTGCAA‑CGTTGGGATCGCCCCTGATCGCCATCACGAGGGGCactgtc > 2:542083/1‑135 (MQ=255) aTCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAA‑CGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTAt < 2:713644/140‑1 (MQ=255) gaTCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAA‑CGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGc < 1:496642/140‑1 (MQ=255) gaTCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAA‑CGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGc < 1:869556/140‑1 (MQ=255) | AAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAA‑CGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGC > NC_000913/646617‑646874 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |