Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	223,648	A→G	intergenic (+240/‑123)	gmhB → / → rrsH	D‑glycero‑beta‑D‑manno‑heptose‑1,7‑bisphosphate 7‑phosphatase/16S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	223,648	0	A	G	100.0%	103.0 / NA	39	intergenic (+240/‑123)	gmhB/rrsH	D‑glycero‑beta‑D‑manno‑heptose‑1,7‑bisphosphate 7‑phosphatase/16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/25);  total (14/25)

ACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGAGCTGAAAGCCGCGTCGCAACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGCATCAAACTTTTAAATTGAAGAGTTT  >  NC_000913/223514‑223784                                                                                                                                       |                                                                                                                                         acaAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGAGCTGAAAGCCGCGTCGCAACTGCTCTTTAACAATTTGTCAGGCAATc                                                                                                                                     >  1:76964/1‑140 (MQ=255) acaAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGAGCTGAAAGCCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATc                                                                                                                                     <  2:381269/140‑1 (MQ=255) acaAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGAGCTGAAAGCCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATc                                                                                                                                     <  2:125588/140‑1 (MQ=255)      cAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGAGCTGAAAGCCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCtgt                                                                                                                                  >  2:406135/1‑138 (MQ=255)      cAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGAGCTGAAAGCCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCtgt                                                                                                                                  <  1:406135/138‑1 (MQ=255)         ccGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGAGCTGAAAGCCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTggg                                                                                                                             <  2:809847/140‑1 (MQ=255)         ccGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGAGCTGAAAGCCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTggg                                                                                                                             <  2:325887/140‑1 (MQ=255)           ggttcggttGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCTCCTCGCGACAGTGAGCTGAAAGCCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCa                                                                                                                           <  2:411461/140‑1 (MQ=255)             ttcggttGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGAGCTGAAAGCCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACt                                                                                                                         <  2:1145737/140‑1 (MQ=255)             ttcggttGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGAGCTGAAAGCCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACt                                                                                                                         >  1:704663/1‑140 (MQ=255)             ttcggttGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGAGCTGAAAGCCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACt                                                                                                                         >  1:507371/1‑140 (MQ=255)                   tGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGAGCTGAAAGCCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGa                                                                                                                   >  2:280901/1‑140 (MQ=25)                      agagAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGAGCTGAAAGCCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATAc                                                                                                                <  2:160544/140‑1 (MQ=17)                      agagAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGAGCTGAAAGCCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATAc                                                                                                                <  1:84687/140‑1 (MQ=17)                       gagaAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGAGCTGAAAGCCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACg                                                                                                               >  1:658593/1‑140 (MQ=17)                       gagaAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGAGCTGAAAGCCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACg                                                                                                               <  1:331101/140‑1 (MQ=17)                                                                                         aGTGAGCTGAAAGCCGCGTCGCAACTGCTCTTTAACAATTTGTCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACa                                             <  2:76964/140‑1 (MQ=11)                                                                                         aGTGAGCTGAAAGCCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACa                                             <  1:623828/140‑1 (MQ=11)                                                                                         aGTGAGCTGAAAGCCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACa                                             <  2:737284/140‑1 (MQ=11)                                                                                         aGTGAGCTGAAAGCCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACa                                             <  1:695208/140‑1 (MQ=11)                                                                                         aGTGAGCTGAAAGCCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACa                                             <  2:433689/140‑1 (MQ=11)                                                                                             aGCTGAAAGCCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGt                                         >  1:893603/1‑140 (MQ=17)                                                                                              gCTGAAAGCCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGtt                                        >  2:1263621/1‑140 (MQ=17)                                                                                                     gCCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCt                                 >  1:108799/1‑140 (MQ=17)                                                                                                       cgcgTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCttt                               <  1:998912/140‑1 (MQ=18)                                                                                                         cgTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGa                             >  1:596964/1‑140 (MQ=17)                                                                                                               aaCTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGCATCa                       <  1:683992/140‑1 (MQ=17)                                                                                                               aaCTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGCATCa                       <  2:30904/140‑1 (MQ=18)                                                                                                                aCTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGCATCaa                      <  2:468328/140‑1 (MQ=18)                                                                                                                    ctctTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGCATCAAACtt                  >  1:196472/1‑140 (MQ=17)                                                                                                                    ctctTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGCATCAAACtt                  <  1:1263621/140‑1 (MQ=18)                                                                                                                    ctctTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGCATCAAACtt                  <  2:440509/140‑1 (MQ=18)                                                                                                                    ctctTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGCATCAAACtt                  <  2:196472/140‑1 (MQ=18)                                                                                                                      ctTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGCATCAAACtttt                <  2:575694/140‑1 (MQ=18)                                                                                                                              aTTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGCATCAAACTTTTAAATTGaa        <  1:1187117/140‑1 (MQ=17)                                                                                                                                 tATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGCATCAAACTTTTAAATTGAagag     >  2:722467/1‑140 (MQ=17)                                                                                                                                 tATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGCATCAAACTTTTAAATTGAagag     >  1:787721/1‑140 (MQ=17)                                                                                                                                 tATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGCATCAAACTTTTAAATTGAagag     >  1:1158140/1‑140 (MQ=17)                                                                                                                                    cAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGCATCAAACTTTTAAATTGAAGAGttt  <  2:596964/140‑1 (MQ=18)                                                                                                                                       |                                                                                                                                         ACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGAGCTGAAAGCCGCGTCGCAACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGCATCAAACTTTTAAATTGAAGAGTTT  >  NC_000913/223514‑223784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑