Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,513,646	T→A	I277N (ATC→AAC)	ydcT →	putative ABC transporter ATP‑binding protein YdcT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,513,646	0	T	A	100.0%	293.3 / NA	85	I277N (ATC→AAC)	ydcT	putative ABC transporter ATP‑binding protein YdcT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (39/46);  total (39/46)

CGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGATCCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGCGAAGAACTGCCTGCCACGCTCACGCCCGGACAA  >  NC_000913/1513512‑1513773                                                                                                                                       |                                                                                                                                cGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAgg                                                                                                                            <  2:823400/140‑1 (MQ=255) cGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAgg                                                                                                                            <  2:431942/140‑1 (MQ=255) cGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAgg                                                                                                                            <  2:1062093/140‑1 (MQ=255) cGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAgg                                                                                                                            <  2:225351/140‑1 (MQ=255)   ggTTCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCg                                                                                                                          >  1:1284440/1‑140 (MQ=255)      tCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTg                                                                                                                       <  1:153438/140‑1 (MQ=255)      tCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTg                                                                                                                       <  2:40221/140‑1 (MQ=255)       cGTTGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGGCCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAgg                                                                                                                            <  1:1068686/134‑1 (MQ=255)       cGTTGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAgg                                                                                                                            >  2:1068686/1‑134 (MQ=255)          tGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGc                                                                                                                           >  1:589566/1‑132 (MQ=255)          tGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGc                                                                                                                           <  2:589566/132‑1 (MQ=255)          tGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAat                                                                                                                   >  1:360507/1‑140 (MQ=255)           ggTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGc                                                                                                                           <  1:414102/131‑1 (MQ=255)           ggTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGc                                                                                                                           >  2:414102/1‑131 (MQ=255)              aCATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCa                                                                                                               >  1:1090831/1‑140 (MQ=255)                  cGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAgg                                                                                                                            >  1:809028/1‑123 (MQ=255)                  cGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAgg                                                                                                                            <  2:809028/123‑1 (MQ=255)                  cGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGgc                                                                                                           >  1:930659/1‑140 (MQ=255)                  cGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGgc                                                                                                           >  1:322124/1‑140 (MQ=255)                    aaTGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGgcgc                                                                                                         >  2:1266464/1‑140 (MQ=255)                      tGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCgg                                                                                                       >  1:133400/1‑140 (MQ=255)                          tttGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAAc                                                                                                   <  1:855414/140‑1 (MQ=255)                          tttGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAAc                                                                                                   <  1:785171/140‑1 (MQ=255)                               tGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGtt                                                                                              <  2:930659/140‑1 (MQ=255)                                  aCTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGtt                                                                                              <  1:178369/137‑1 (MQ=255)                                  aCTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGtt                                                                                              >  2:178369/1‑137 (MQ=255)                                  aCTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTg                                                                                           >  2:947039/1‑140 (MQ=255)                                  aCTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTg                                                                                           >  2:842372/1‑140 (MQ=255)                                  aCTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTg                                                                                           >  1:560448/1‑140 (MQ=255)                                   cTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGa                                                                                          <  2:360507/140‑1 (MQ=255)                                      aTGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACt                                                                                       >  1:209240/1‑140 (MQ=255)                                        ggCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGa                                                                                     >  2:403878/1‑140 (MQ=255)                                             agaAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTg                                                                                >  1:166033/1‑140 (MQ=255)                                                  cTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAAcgg                                                                           <  2:560448/140‑1 (MQ=255)                                                    ttGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAAcggcg                                                                         <  2:1090831/140‑1 (MQ=255)                                                     tgtgGCATGACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAAcggcgg                                                                        >  2:54773/1‑140 (MQ=255)                                                       tgGCATGACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTg                                                                      >  1:842957/1‑140 (MQ=255)                                                        gagaTGCCGGTAAGCTTCGCCCTCCGACCGGACCATAACCGGCGCAACAGCCATGGTGAACTGCAGGCCAATGGCTCGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGa                                                                     <  1:799885/137‑1 (MQ=255)                                                         gCATGACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGaa                                                                    >  2:512389/1‑140 (MQ=255)                                                          cATGACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGaaa                                                                   <  1:512389/140‑1 (MQ=255)                                                            tGACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGaaaaa                                                                 >  1:311730/1‑140 (MQ=255)                                                            tGACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGaaaaa                                                                 <  2:209240/140‑1 (MQ=255)                                                             gACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAAcgg                                                                           >  2:789002/1‑129 (MQ=255)                                                             gACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAAcgg                                                                           <  1:789002/129‑1 (MQ=255)                                                             gACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAAc                                                                >  1:533935/1‑140 (MQ=255)                                                               ggggAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTg                                                              <  1:640956/139‑1 (MQ=255)                                                                   aaGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTtg                                                          <  1:677742/140‑1 (MQ=255)                                                                    aGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTtgt                                                         <  1:47876/140‑1 (MQ=255)                                                                    aGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTtgt                                                         <  1:66196/140‑1 (MQ=255)                                                                        tCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGTGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGt                                                     <  2:702911/140‑1 (MQ=255)                                                                        tCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGt                                                     <  1:1266464/140‑1 (MQ=255)                                                                        tCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGt                                                     <  2:166033/140‑1 (MQ=255)                                                                         cGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGt                                                     <  1:841365/139‑1 (MQ=255)                                                                         cGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGt                                                     >  2:841365/1‑139 (MQ=255)                                                                         cGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGt                                                     <  2:843130/139‑1 (MQ=255)                                                                         cGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGt                                                     >  1:843130/1‑139 (MQ=255)                                                                           cccTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAg                                                  <  2:1284440/140‑1 (MQ=255)                                                                           cccTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAg                                                  <  2:311730/140‑1 (MQ=255)                                                                           cccTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAg                                                  <  2:322124/140‑1 (MQ=255)                                                                           cccTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAg                                                  <  1:842372/140‑1 (MQ=255)                                                                           cccTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAg                                                  >  2:70841/1‑140 (MQ=255)                                                                                cGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGt                                                     >  1:551943/1‑132 (MQ=255)                                                                                cGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGt                                                     <  2:551943/132‑1 (MQ=255)                                                                                     ggAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGa                                        <  2:133400/140‑1 (MQ=255)                                                                                      gacagtatCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGAc                                       <  1:265574/135‑1 (MQ=255)                                                                                        aCATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAg                                     <  1:70841/140‑1 (MQ=255)                                                                                          atatCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAggg                                   >  1:808407/1‑139 (MQ=255)                                                                                          atatCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGc                                   <  2:533935/140‑1 (MQ=255)                                                                                          atatCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGc                                   <  2:1021453/140‑1 (MQ=255)                                                                                          atatCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGc                                   >  1:235469/1‑140 (MQ=255)                                                                                               cGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGCgaaga                              <  1:947039/140‑1 (MQ=255)                                                                                                 ccTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGCGAAGAAc                            >  1:1034539/1‑140 (MQ=255)                                                                                                 ccTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGCGAAGAAc                            >  1:826052/1‑140 (MQ=255)                                                                                                         ccccTGGTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGCGAAGAACtgcctgcc                    <  1:54773/140‑1 (MQ=255)                                                                                                              ggTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGAGAAAAACTGCTTTTGGGTCAAGCCAAAATTCGAGGCGAAAAACGGCCTGCCCCGcc               >  2:1209799/1‑139 (MQ=255)                                                                                                               gTGAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGCGAAGAACTGCCTGCCACGCTc              >  2:74613/1‑140 (MQ=255)                                                                                                                 gAACTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAAATGCTTGTGAGGCAGGCCAAAAAGACAGACGGAAAAGTTCCCGCCACGCCCAc            >  1:643956/1‑140 (MQ=255)                                                                                                                    cTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGCGAAGAACTGCCTGCCACGCt               >  1:1257726/1‑134 (MQ=255)                                                                                                                    cTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGCGAAGAACTGCCTGCCACGCt               <  2:1257726/134‑1 (MQ=255)                                                                                                                    cTGCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGCGAAGAACTGCCTGCCACGCTCACGcc         >  2:1179795/1‑140 (MQ=255)                                                                                                                      gCAGGCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGCGAAGAACTGCCTGCCACGCTCACGCCCg       <  2:842957/140‑1 (MQ=255)                                                                                                                         ggCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGGGGAGAACTGCCTGCCACGCTCACGCCCgggc    >  1:917271/1‑138 (MQ=255)                                                                                                                         ggCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGCGAAGAACTGCCTGCCACGCTCACGCCCGGa     <  1:503370/139‑1 (MQ=255)                                                                                                                         ggCCAATGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGCGAAGAAATGCCTGCCACGCTCACGCCCGGa     >  2:503370/1‑139 (MQ=255)                                                                                                                               tGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGCGAAGAACTGCCTGCCACGCTCACGCCCGGacaa  <  1:155986/136‑1 (MQ=255)                                                                                                                               tGGCACGAACCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGCGAAGAACTGCCTGCCACGCTCACGCCCGGacaa  >  2:155986/1‑136 (MQ=255)                                                                                                                                       |                                                                                                                                CGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAACATATCCGCCTCAACACCCCTGGTGAACTGCAGGCCAATGGCACGATCCAGGCGGTGCAATATCAGGGCGCGGCAACTCGTTTTGAACTGAAATTGAACGGCGGTGAAAAACTGCTTGTGAGTCAGGCCAATATGACAGGCGAAGAACTGCCTGCCACGCTCACGCCCGGACAA  >  NC_000913/1513512‑1513773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑