Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,077,978	A→C	A294A (GCT→GCG)	cmtA ←	mannitol‑specific PTS enzyme IICB component CmtA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,077,978	0	A	C	100.0%	281.7 / NA	72	A294A (GCT→GCG)	cmtA	mannitol‑specific PTS enzyme IICB component CmtA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (39/33);  total (39/33)

GTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGCAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCAGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGCCAGACATACCGCCCGCAATCATGGCAATAATGGTCAGCGGCTTCATCAGCACATACGGGAAATACAGTTCGTGGATCCCACCGAGGAAGTGAATA  >  NC_000913/3077841‑3078113                                                                                                                                          |                                                                                                                                        gTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGCAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGgcccgc                                                                                                                                       >  2:613996/1‑140 (MQ=255)   tttttCCATCTTCAGTATCAGCGAAGTAATAGCAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGgcccgcca                                                                                                                                     <  2:956672/140‑1 (MQ=255)      ttCCATCTTCAGTATCAGCGAAGTAATAGCAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGccacca                                                                                                                                  <  1:1274907/140‑1 (MQ=255)        ccATCTTCAGTATCAGCGAAGTAATAGCAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGa                                                                                                                                <  2:1237949/140‑1 (MQ=255)                  tATCAGCGAAGTAATAGCAAAGGACACCAGGGTACCTACCGCAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCa                                                                                                                      <  2:45888/140‑1 (MQ=255)                   aTCAGCGAAGTAATAGCAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAg                                                                                                                     >  1:1160260/1‑140 (MQ=255)                       gCGAAGTAATAGCAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGac                                                                                                                               >  1:823310/1‑126 (MQ=255)                       gCGAAGTAATAGCAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGac                                                                                                                               <  2:823310/126‑1 (MQ=255)                       gCGAAGTAATAGCAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCa                                                                                                                      <  1:838769/135‑1 (MQ=255)                       gCGAAGTAATAGCAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCa                                                                                                                      >  2:838769/1‑135 (MQ=255)                       gCGAAGTAATAGCAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTa                                                                                                                   <  1:514290/138‑1 (MQ=255)                       gCGAAGTAATAGCAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTa                                                                                                                   >  2:514290/1‑138 (MQ=255)                       gCGAAGTAATAGCAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAg                                                                                                                 >  2:1022659/1‑140 (MQ=255)                        cGAAGTAATAGCAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGt                                                                                                                >  2:765892/1‑140 (MQ=255)                          aaGTAATAGCAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTa                                                                                                              >  2:185254/1‑140 (MQ=255)                                 aGCAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATcc                                                                                                       <  1:765892/140‑1 (MQ=255)                                 aGCAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATcc                                                                                                       <  1:613996/140‑1 (MQ=255)                                     aaGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGt                                                                                                   <  1:179149/140‑1 (MQ=255)                                     aaGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGt                                                                                                   >  1:894837/1‑140 (MQ=255)                                       ggACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGc                                                                                                 >  2:329672/1‑140 (MQ=255)                                        gACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAg                                                                                                                 <  2:1150184/123‑1 (MQ=255)                                        gACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAg                                                                                                                 >  1:1150184/1‑123 (MQ=255)                                        gACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGcc                                                                                                >  2:1197227/1‑140 (MQ=255)                                           acCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGCCAGa                                                                                             >  1:513435/1‑140 (MQ=255)                                                ggTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGCCAGACATAc                                                                                        <  1:185254/140‑1 (MQ=255)                                                ggTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGCCAGACATAc                                                                                        <  1:1074860/140‑1 (MQ=255)                                                  tacctaccGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGCCAGACATAccg                                                                                      >  2:495057/1‑140 (MQ=255)                                                    cctaccGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAg                                                                                                     <  2:259742/123‑1 (MQ=255)                                                    cctaccGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAg                                                                                                     >  1:259742/1‑123 (MQ=255)                                                       accGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGCCAGACATAccgcccgc                                                                                 >  1:1110553/1‑140 (MQ=255)                                                        ccGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGCCAGACATACCGCCCGCa                                                                                <  2:1160260/140‑1 (MQ=255)                                                           tAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGCCAGACATACCGCCCGCAATc                                                                             >  1:1031004/1‑140 (MQ=255)                                                               cccGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGCCAGACATACCGCCCGCAATCATgg                                                                         <  2:855635/139‑1 (MQ=255)                                                               acCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGCCAGACATACCGCCCGCAATCATgg                                                                         <  2:397850/140‑1 (MQ=255)                                                               acCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGCCAGACATACCGCCCGCAATCATgg                                                                         <  2:107711/140‑1 (MQ=255)                                                               acCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGCCAGACATACCGCCCGCAATCATgg                                                                         <  2:669898/140‑1 (MQ=255)                                                                 cGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGCCAGACATACCGCCCGCAATCATGGCa                                                                       >  1:553486/1‑140 (MQ=255)                                                                   gCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGCCAGACATACCGCCCGCAATCATGGCaat                                                                     >  2:581454/1‑140 (MQ=255)                                                                    cAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGCCAGACATACCGCCCGCAATCATGGCaata                                                                    <  1:581454/140‑1 (MQ=255)                                                                       ttGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGCCAGACATACCGCCCGCAATCATGGCAATAATg                                                                 <  1:495057/140‑1 (MQ=255)                                                                           cGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGCCAGACATACCGCCCGCAATCATGGCAATAATGGTCa                                                             >  1:1223506/1‑140 (MQ=255)                                                                            gCCAGGAACGAGCCTTTCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGCCAGACATACCGCCCGCAATCATGGCAATAATGGTCAg                                                            >  1:104892/1‑140 (MQ=255)                                                                            gCCAGGAACGAGCCTTTCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGCCAGACATACCGCCCGCAATCATGGCAATAATGGTCAg                                                            >  2:292894/1‑140 (MQ=255)                                                                                ggAACGAGCCTTTCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGCCAGACATACCGCCCGCAATCATGGCAATAATGGTCAGCGGc                                                        <  2:894837/140‑1 (MQ=255)                                                                                   aCGAGCCTTTCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCGGGTGCCAGACATACCGCCCGCAATCATGGCAATAATGGTCAGCGGCTtc                                                     <  1:1022659/140‑1 (MQ=255)                                                                                      aGCCTTTCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGCCAGACATACCGCCCGCAATCATGGCAATAATGGTCAGCGGCTtcatc                                                  >  2:961808/1‑140 (MQ=255)                                                                                          tttCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGCCAGACATACCGCCCGCAATCATGGCAATAATGGTCAGCGGCTTCATCAGca                                              >  1:403448/1‑140 (MQ=255)                                                                                           ttCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGCCAGACATACCGCCCGCAATCATGGCAATAATGGTCAGCGGCTTCATCAGcac                                             >  2:945252/1‑140 (MQ=255)                                                                                           ttCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGCCAGACATACCGCCCGCAATCATGGCAATAATGGTCAGCGGCTTCATCAGcac                                             >  1:825929/1‑140 (MQ=255)                                                                                             cGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGCCAGACATACCGCCCGCAATCATGGCAATAATGGTCAGCGGCTTCATCAGCACAt                                           <  2:553486/140‑1 (MQ=255)                                                                                                 gTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGCCAGACATACCGCCCGCAATCATGGCAATAATGGTCAGCGGCTTCATCAGCACATACgg                                       <  2:104892/140‑1 (MQ=255)                                                                                                       gCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGCCAGACATACCGCCCGCAATCATGGCAATAATGGTCAGCGGCTTCATCAGCACATACGGGAAATa                                 <  1:292894/140‑1 (MQ=255)                                                                                                       gCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGCCAGACATACCGCCCGCAATCATGGCAATAATGGTCAGCGGCTTCATCAGCACATACGGGAAATa                                 <  2:403448/140‑1 (MQ=255)                                                                                                             tAAGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGCCAGACATACCGCCCGCAATCATGGCAATAATGGTCAGCGGCTTCATCAGCACATACGGGAAATACAGTTc                           <  1:945252/140‑1 (MQ=255)                                                                                                              aaGCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGCCAGACATACCGCCCGCAATCATGGCAATAATGGTCAGCGGCTTCATCAGCACATACGGGAAATACAGTTCg                          <  1:329672/140‑1 (MQ=255)                                                                                                                gCAAAGATAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGCCAGACATACCGCCCGCAATCATGGCAATAATGGTCAGCGGCTTCATCAGCACATACGGGAAATACAGTTCGTg                        >  1:504878/1‑140 (MQ=255)                                                                                                                      aTAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGCCAGACATACCGCCCGCAATCATGGCAATAATGGTCAGCGGCTTCATCAGCACATACGGGAAATACAGTTCGTGGATccc                  >  2:656207/1‑140 (MQ=255)                                                                                                                      aTAGACCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGCCAGACATACCGCCCGCAATCATGGCAATAATGGTCAGCGGCTTCATCAGCACATACGGGAAATACAGTTCGTGGATccc                  >  2:485327/1‑140 (MQ=255)                                                                                                                          aCCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGCCAGACATACCGCCCGCAATCATGGCAATAATGGTCAGCGGCTTCATCAGCACATACGGGAAATACAGTTCGTGGATCCCACCg              <  2:504878/140‑1 (MQ=255)                                                                                                                          aCCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGCCAGACATACCGCCCGCAATCATGGCAATAATGGTCAGCGGCTTCATCAGCACATACGGGAAATACAGTTCGTGGATCCCACCg              >  2:544160/1‑140 (MQ=255)                                                                                                                          aCCCCGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGCCAGACATACCGCCCGCAATCATGGCAATAATGGTCAGCGGCTTCATCAGCACATACGGGAAATACAGTTCGTGGATCCCACCg              >  2:760273/1‑140 (MQ=255)                                                                                                                           ccccGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGCCAGACATACCGCCCGCAATCATGGCAATAATGGTCAGCGGCTTCATCAGCACATACGGGAAATACAGTTCGTGGATCCCACCGa             >  1:1199923/1‑140 (MQ=255)                                                                                                                           ccccGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGCCAGACATACCGCCCGCAATCATGGCAATAATGGTCAGCGGCTTCATCAGCACATACGGGAAATACAGTTCGTGGATCCCACCGa             >  1:1031464/1‑140 (MQ=255)                                                                                                                             ccGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGCCAGACATACCGCCCGCAATCATGGCAATAATGGTCAGCGGCTTCATCAGCACATACGGGAAATACAGTTCGTGGATCCCACCGAgg           <  2:1110553/140‑1 (MQ=255)                                                                                                                              cGGACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGCCAGACATACCGCCCGCAATCATGGCAATAATGGTCAGCGGCTTCATCAGCACATACGGGAAATACAGTTCGTGGATCCCACCGAGGa          <  2:1223506/140‑1 (MQ=255)                                                                                                                               ggACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGCCAGACATACCGCCCGCAATCATGGCAATAATGGTCAGCGGCTTCATCAGCACATACGGGAAATACAGTTCGTGGATCCCACCGAGGaa         >  1:129568/1‑140 (MQ=255)                                                                                                                                gACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGCCAGACATACCGCCCGCAATCATGGCAATAATGGTCAGCGGCTTCATCAGCACATACGGGAAATACAGTTCGTGGATCCCACCGAGGAAg        <  1:961808/140‑1 (MQ=255)                                                                                                                                gACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGCCAGACATACCGCCCGCAATCATGGCAATAATGGTCAGCGGCTTCATCAGCACATACGGGAAATACAGTTCGTGGATCCCACCGAGGAAg        >  2:1036933/1‑140 (MQ=255)                                                                                                                                gACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGCCAGACATACCGCCCGCAATCATGGCAATAATGGTCAGCGGCTTCATCAGCACATACGGGAAATACAGTTCGTGGATCCCACCGAGGAAg        >  2:372948/1‑140 (MQ=255)                                                                                                                                gACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGCCAGACATACCGCCCGCAATCATGGCAATAATGGTCAGCGGCTTCATCAGCACATACGGGAAATACAGTTCGTGGATCCCACCGAGGAAg        >  2:1057180/1‑140 (MQ=255)                                                                                                                                gACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGCCAGACATACCGCCCGCAATCATGGCAATAATGGTCAGCGGCTTCATCAGCACATACGGGAAATACAGTTCGTGGATCCCACCGAGGAAg        >  2:1150167/1‑140 (MQ=255)                                                                                                                                gACTTGGGCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGCCAGACATACCGCCCGCAATCATGGCAATAATGGTCAGCGGCTTCATCAGCACATACGGGAAATACAGTTCGTGGATCCCACCGAGGAAg        >  1:1113257/1‑140 (MQ=255)                                                                                                                                     gggCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGCCAGACATACCGCCCGCAATCATGGCAATAATGGTCAGCGGCTTCATCAGCACATACGGGAAATACAGTTCGTGGATCCCACCGAGGAAGTGaat   <  2:825929/140‑1 (MQ=255)                                                                                                                                      ggCCCGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGCCAGACATACCGCCCGCAATCATGGCAATAATGGTCAGCGGCTTCATCAGCACATACGGGAAATACAGTTCGTGGATCCCACCGAGGAAGTGaata  >  1:286696/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        GTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGCAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAGGTAAGCAAAGATAGACCCCGGACTTGGGCCAGCCACCAGACCACCGTCCAGTAAGTTAAACATCCAGGTGCCAGACATACCGCCCGCAATCATGGCAATAATGGTCAGCGGCTTCATCAGCACATACGGGAAATACAGTTCGTGGATCCCACCGAGGAAGTGAATA  >  NC_000913/3077841‑3078113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑