Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,059,312	C→T	G19G (GGC→GGT)	torA →	trimethylamine N‑oxide reductase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,059,312	0	C	T	100.0%	199.4 / NA	57	G19G (GGC→GGT)	torA	trimethylamine N‑oxide reductase 1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/28);  total (29/28)

CGCACCTTGTTGAAATATCTGCAAATGAATGCGTCTGACACCGCAGGTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGCGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGACTGCGGCGCAAGCGGCGACTGACGCTGTCATCTCGAAAGAGGGCATTCTTACCGGGTCGCACTGGGGGGCTATCCGCGCGA  >  NC_000913/1059176‑1059448                                                                                                                                         |                                                                                                                                         cGCACCTTGTTGAAATATCTGCAAATGAATGCGTCTGACACCGCAGGTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCggtggc                                                                                                                                       >  2:77139/1‑140 (MQ=255)  gCACCTTGTTGAAATATCTGCAAATGAATGCGTCTGACACCGCAGGTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGTGGCt                                                                                                                                      <  1:619541/140‑1 (MQ=255)      cTTGTTGAAATATCTGCAAATGAATGCGTCTGACACCGCAGGTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGTGGCTTAAc                                                                                                                                  >  2:212029/1‑140 (MQ=255)         gttgAAATATCTGCAAATGAATGCGTCTGACACCGCAGGTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGTGGCTTAACCGt                                                                                                                               <  2:302148/140‑1 (MQ=255)             aaaTATCTGCAAATGAATGCGTCTGACACCGCAGGTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGTGGCTTAACCGTCGcc                                                                                                                           <  1:2606/140‑1 (MQ=255)               atatCTGCAAATGAATGCGTCTGACACCGCAGGTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGTGGCTTAACCGTCGCCgg                                                                                                                         >  1:581686/1‑140 (MQ=255)                 atCTGCAAATGAATGCGTCTGACACCGCAGGTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGTGGCTTAACCGTCGCCGGGa                                                                                                                       <  1:212029/140‑1 (MQ=255)                 atCTGCAAATGAATGCGTCTGACACCGCAGGTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGTGGCTTAACCGTCGCCGGGa                                                                                                                       <  1:1255810/140‑1 (MQ=255)                  tCTGCAAATGAATGCGTCTGACACCGCAGGTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGTGGCTTAACCGTCGCCg                                                                                                                          >  2:1061675/1‑136 (MQ=255)                  tCTGCAAATGAATGCGTCTGACACCGCAGGTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGTGGCTTAACCGTCGCCg                                                                                                                          <  1:1061675/136‑1 (MQ=255)                  tCTGCAAATGAATGCGTCTGACACCGCAGGTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGTGGCTTAACCGTCGCCGGGAt                                                                                                                      >  2:547309/1‑140 (MQ=255)                    tGCAAATGAATGCGTCTGACACCGCAGGTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGTGGCTTAACCGTCGCCGGGATGc                                                                                                                    >  1:1046758/1‑140 (MQ=255)                     gCAAATGAATGCGTCTGACACCGCAGGTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGTGGCTTAACCGTCGCCGGGATGCt                                                                                                                   <  1:1149540/140‑1 (MQ=255)                        aatgaatgCGTCTGACACCGCAGGTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGTGGCTTAACCGTCGCCGGGATGCTggg                                                                                                                <  1:769380/140‑1 (MQ=255)                        aatgaatgCGTCTGACACCGCAGGTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGTGGCTTAACCGTCGCCGGGATGCTggg                                                                                                                <  2:1046758/140‑1 (MQ=255)                         atgaatgCGTCTGACACCGCAGGTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGTGGCTTAACCGTCGCCGGGATGCTgggg                                                                                                               <  1:77139/140‑1 (MQ=255)                         atgaatgCGTCTGACACCGCAGGTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGTGGCTTAACCGTCGCCGGGATGCTgggg                                                                                                               <  1:448782/140‑1 (MQ=255)                          tgaatgCGTCTGACACCGCAGGTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGTGGCTTAACCGTCGCCGGGATGCTGGGGc                                                                                                              <  1:547309/140‑1 (MQ=255)                           gaatgCGTCTGACACCGCAGGTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGTGGCTTAACCGTCGCCGGGATGCTGGGGcc                                                                                                             <  1:648305/140‑1 (MQ=255)                                     gACACCGCAGGTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGTGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTa                                                                                                   >  1:695714/1‑140 (MQ=255)                                        acCGCAGGTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGTGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAAcg                                                                                                <  1:1182457/140‑1 (MQ=255)                                          cGCAGGTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGTGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAAcgcc                                                                                              >  2:589637/1‑140 (MQ=255)                                           gCAGGTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGTGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAAcgccg                                                                                             <  1:948462/140‑1 (MQ=255)                                            cAGGTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGTGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAAcgccgc                                                                                            >  1:294517/1‑140 (MQ=255)                                            cAGGTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGTGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAAcgccgc                                                                                            >  1:1219910/1‑140 (MQ=255)                                               gTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAACAACAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGTGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAAcgccg                                                                                             <  2:311378/136‑1 (MQ=255)                                               gTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAACAACAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGTGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAAcgccg                                                                                             >  1:311378/1‑136 (MQ=255)                                                  aGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGTGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAAcgccg                                                                                             <  1:224868/133‑1 (MQ=255)                                                  aGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGTGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAAcgccg                                                                                             >  2:224868/1‑133 (MQ=255)                                                       cACGGCGATAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGTGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGACt                                                                                 >  2:465418/1‑140 (MQ=255)                                                         cGGCGATAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGTGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGACTgc                                                                               >  1:1008557/1‑140 (MQ=255)                                                              aTAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGTGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGACTGCGgcgc                                                                          >  2:377467/1‑140 (MQ=255)                                                              aTAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGCATCACGACGGCGTTTTCTGGCACAACTCGGTGGCTTAACCGTCGCCGGGATGCTGGGCCCGTCATTGTTAACGCCGCGACGTGCGACTGCGgcgc                                                                          >  1:720833/1‑140 (MQ=255)                                                                    aggaagAAAAATAATGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGTGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGACTGCGGCGCAAgcgg                                                                    >  1:425572/1‑140 (MQ=255)                                                                                 atGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGTGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGACTGCGGCGCAAGCGGCGACTGACGCTGt                                                       <  1:1215875/140‑1 (MQ=255)                                                                                 atGAACAATAACGATCTCTTGCAGGCATCGCGTCGGCGTTTTCTGGCACAGCTCGGTGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGACTGCGGCGCAAGCGGCGACTGACGCTGt                                                       <  2:720833/140‑1 (MQ=255)                                                                                              aTCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGTGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGACTGCGGCGCAAGCGGCGACTGACGCTGTCATCTCGAAagag                                          >  2:425404/1‑140 (MQ=255)                                                                                              aTCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGTGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGACTGCGGCGCAAGCGGCGACTGACGCTGTCATCTCGAAagag                                          <  2:425572/140‑1 (MQ=255)                                                                                              aTCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGTGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGACTGCGGCGCAAGCGGCGACTGACGCTGTCATCTCGAAagag                                          >  2:263241/1‑140 (MQ=255)                                                                                                ctctTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGTGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGACTGCGGCGCAAGCGGCGACTGACGCTGTCATCTCGAAAGAggg                                        >  1:1026419/1‑140 (MQ=255)                                                                                                   tttCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGTGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGACTGCGGCGCAAGCGGCGACTGACGCTGt                                                       <  2:1141019/122‑1 (MQ=255)                                                                                                   tttCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGTGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGACTGCGGCGCAAGCGGCGACTGACGCTGt                                                       >  1:1141019/1‑122 (MQ=255)                                                                                                   tttCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGTGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGACTGCGGCGCAAGCGGCGACTGACGCTGTCATCTCGAAAGAGGGCAt                                     >  2:11485/1‑140 (MQ=255)                                                                                                   tttCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGTGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGACTGCGGCGCAAGCGGCGACTGACGCTGTCATCTCGAAAGAGGGCAt                                     >  1:845881/1‑140 (MQ=255)                                                                                                        ggCATCACGTCGGCGTTTTCTGGCACAACTCGGTGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGACTGCGGCGCAAGCGGCGACTGACGCTGTCATCTCGAAAGAGGGCATTCTTa                                >  2:1210222/1‑140 (MQ=255)                                                                                                         gCATCACGTCGGCGTTTTCTGGCACAACTCGGTGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGACTGCGGCGCAAGCGGCGACTGACGCTGTCATCTCGAAAGAGGGCATTCTTAc                               <  1:189207/140‑1 (MQ=255)                                                                                                           aTCACGTCGGCGTTTTCTGGCACAACTCGGTGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGACTGCGGCGCAAGCGGCGACTGACGCTGTCATCTCGAAAGAGGGCATTCTTACCg                             >  1:229446/1‑140 (MQ=255)                                                                                                             cACGTCGGCGTTTTCTGGCACAACTCGGTGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGACTGCGGCGCAAGCGGCGACTGACGCTGTCATCTCGAAAGAGGGCATTCTTACCggg                           >  2:51891/1‑140 (MQ=255)                                                                                                               cGTCGGCGTTTTCTGGCACAACTCGGTGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGACTGCGGCGCAAGCGGCGACTGACGCTGTCATCTCGAAAGAGGGCATTCTTACCGGGTc                         <  2:845881/140‑1 (MQ=255)                                                                                                                  cGGCGTTTTCTGGCACAACTCGGTGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGACTGCGGCGCAAGCGGCGACTGACGCTGTCATCTCGAAAGAGGGCATTCTTACCGGGTCGCa                      <  1:11485/140‑1 (MQ=255)                                                                                                                   ggCGTTTTCTGGCACAACTCGGTGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGACTGCGGCGCAAGCGGCGACTGACGCTGTCATCTCGAAAGAGGGCATTCTTACCGGGTCGCAc                     >  1:371970/1‑140 (MQ=255)                                                                                                                    gCGTTTTCTGGCACAACTCGGTGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGACTGCGGCGCAAGCGGCGACTGACGCTGTCATCTCGAAAGAGGGCATTCTTACCGGGTCGCACt                    <  1:178567/140‑1 (MQ=255)                                                                                                                    gCGTTTTCTGGCACAACTCGGTGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGACTGCGGCGCAAGCGGCGACTGACGCTGTCATCTCGAAAGAGGGCATTCTTACCGGGTCGCACt                    >  1:14094/1‑140 (MQ=255)                                                                                                                     cGTTTTCTGGCACAACTCGGTGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGACTGCGGCGCAAGCGGCGACTGACGCTGTCATCTCGAAAGAGGGCATTCTTACCGGGTCGCACTg                   >  2:232941/1‑140 (MQ=255)                                                                                                                            tGGCACAACTCGGTGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGACTGCGGCGCAAGCGGCGACTGACGCTGTCATCTCGAAAGAGGGCATTCTTACCGGGTCGCACTGGGGGGCt            <  2:14094/140‑1 (MQ=255)                                                                                                                                    cTCGGTGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGACTGCGGCGCAAGCGGCGACTGACGCTGTCATCTCGAAAGAGGGCATTCTTACCGGGTCGCACTGGGGGGCTATCcgcgc    >  1:1077229/1‑140 (MQ=255)                                                                                                                                    cTCGGTGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGACTGCGGCGCAAGCGGCGACTGACGCTGTCATCTCGAAAGAGGGCATTCTTACCGGGTCGCACTGGGGGGCTATCcgcgc    <  2:229446/140‑1 (MQ=255)                                                                                                                                     tCGGTGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGACTGCGGCGCAAGCGGCGACTGACGCTGTCATCTCGAAAGAGGGCATTCTTACCGGGTCGCACTGGGGGGCTATCcgcgcg   <  2:1219910/140‑1 (MQ=255)                                                                                                                                     tCGGTGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGACTGCGGCGCAAGCGGCGACTGACGCTGTCATCTCGAAAGAGGGCATTCTTACCGGGTCGCACTGGGGGGCTATCcgcgcg   <  1:425404/140‑1 (MQ=255)                                                                                                                                      cggtggCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGACTGCGGCGCAAGCGGCGACTGACGCTGTCATCTCGAAAGAGGGCATTCTTACCGGGTCGCACTGGGGGGCTATCCGCGCGa  <  1:232941/140‑1 (MQ=255)                                                                                                                                      cggtggCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGACTGCGGCGCAAGCGGCGACTGACGCTGTCATCTCGAAAGAGGGCATTCTTACCGGGTCGCACTGGGGGGCTATCCGCGCGa  <  2:371970/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         CGCACCTTGTTGAAATATCTGCAAATGAATGCGTCTGACACCGCAGGTAAGGCTCACGGCGATAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGCGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGACTGCGGCGCAAGCGGCGACTGACGCTGTCATCTCGAAAGAGGGCATTCTTACCGGGTCGCACTGGGGGGCTATCCGCGCGA  >  NC_000913/1059176‑1059448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑