Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,315,9360TA26.7% 93.3 / 11.9 45K34* (AAA→TAA) yciGprotein YciG
Reads supporting (aligned to +/- strand):  ref base T (26/7);  new base A (0/12);  total (26/19)
Fisher's exact test for biased strand distribution p-value = 1.75e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCATGTTATTTCTCCCGTTGCGTTGCATTGTTTCATTAATATG  >  NC_000913/1315799‑1316074
                                                                                                                                         |                                                                                                                                          
cATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTaa                                                                                                                                          >  1:809978/1‑140 (MQ=255)
      gCCCGCAGATGAAATTATTTTTATCAGTTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTCCCCCCGCTTTGTTGACCGCTTTTTTTCCCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTAc                                                                                                                                    <  2:809978/140‑1 (MQ=255)
       cccGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTAcc                                                                                                                                   >  1:779661/1‑140 (MQ=255)
         cGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTAccgc                                                                                                                                 >  2:511163/1‑140 (MQ=255)
          gCAGATGCATTTTTCTTTATCAGCTGATTTTAATAATTACCGGTGAATCAGGTTTTGCTTGTTTTATTCCCCCCGTTTTGTGGCCCGCTTTTTTTCCCCGCTTCAGATGCGCGTTGCGGTTCTTTTTAAAAATTAccgcc                                                                                                                                <  2:739727/140‑1 (MQ=255)
                            aTCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCttt                                                                                                              >  1:77245/1‑140 (MQ=255)
                                 ttGATATTAATAATAAGCGGTGAATCAGTTTTGGCCTGTTTTATCCCCCCCGTTTTGTTGCCCGCTTTTTTTCCCCGCTTCAGATGCGCGTGGCGGATCTTTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGc                                                                                                         <  2:271072/139‑1 (MQ=255)
                                   gATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCt                                                                                                       >  2:106930/1‑140 (MQ=255)
                                   aatatTAATAATTACGGGTAAATCAGGATTTGCCTGATTTATTCCCCCCTTTTTGTTGCCCGCTTTTTTTCCCCGCTTCAGATGCGCGTTGCGGATCTTTTTAAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCt                                                                                                       <  2:595328/139‑1 (MQ=255)
                                         aataatTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCg                                                                                                 >  1:890475/1‑140 (MQ=255)
                                          ataatTAATTGTAAATAAGGTTTTCCTTGTTTTATTCCCCCCTTTTTGTTGCCCGCTTTTTTTCCCCCCTTAAGAGGCGCGTTGGGGATTTTTTTAAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCgg                                                                                                <  2:995108/140‑1 (MQ=255)
                                           aaatTACGGGTAAATGAGTATTTGCCTTTTTTATTCCCCCCGTTTTGTTCCCCCCTTTTTTCCCCCGCTTCAGATGCGCTTTGCGAATCTTTTTAAAAATTCCCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGa                                                                                               <  2:455944/139‑1 (MQ=255)
                                               tAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGcc                                                                                           >  1:332028/1‑140 (MQ=255)
                                                     gaaaTCAGGTTTTGCTTGTTTTATTCCCCCCGTTTTGTTGACCCCTTTTTTCCCCCGCTTCAGATGCGCGTTGCGGATTTTTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCa                                                                                     <  2:332028/138‑1 (MQ=255)
                                                       aaTCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACg                                                                                   >  2:460579/1‑140 (MQ=255)
                                                                   ccTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGaa                                                                       >  1:524888/1‑140 (MQ=255)
                                                                       aTTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAAttt                                                                   >  2:988576/1‑140 (MQ=255)
                                                                       aTTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAAttt                                                                   >  1:17776/1‑140 (MQ=255)
                                                                                  ccGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAaccacc                                                        >  2:623354/1‑140 (MQ=255)
                                                                                     ttttGTTGCCCGCTTTTTTCCCCCTCTTCAGATGCGCGTTGCGGATCTTTTTAAAAATTCCCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACg                                                     <  2:894129/139‑1 (MQ=255)
                                                                                      tttGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGa                                                    >  1:772176/1‑140 (MQ=255)
                                                                                             cccGCTTTTTTTCCCCGCTTCAGATGCGCGTTGCGGATTTTTTTAAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCgg                                             <  2:779661/139‑1 (MQ=255)
                                                                                               ccccTTTTTTCCCCGCTTCAGATGCGCGTTGGGGATCTTTTTAAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGTGAAATTTCCTGAACCACCACGATGTTCGGcc                                           <  2:67327/138‑1 (MQ=255)
                                                                                                gCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCa                                          >  1:347028/1‑140 (MQ=255)
                                                                                                 ccTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCAt                                         >  1:111321/1‑140 (MQ=255)
                                                                                                      tttCCCCGCTTCAGAGGCGCGTTGCGGTTCTTTTTAAAATTTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCATGTTAt                                    <  2:564721/140‑1 (MQ=255)
                                                                                                      ttcccccGCTTCAGATGCGGGTTGCGAATCTTTTTAAAAATTCCCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCATGTTAt                                    <  2:212840/136‑1 (MQ=255)
                                                                                                             gCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCATGTTATTTCTccc                             >  1:564721/1‑140 (MQ=255)
                                                                                                              tttCAGTTGCGCTTGGCGGTTCTTTTTAAAATTTCCCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCATGTTATTTCTCCcg                            <  2:111321/139‑1 (MQ=255)
                                                                                                              tttCAGATGCGTTTTGCGGATTTTTTTAAAATTTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCATGTTATTTCTCCcg                            <  2:966502/139‑1 (MQ=255)
                                                                                                              cTTCAGATGCGCTTTGGGGATTTTTTTAAAATTTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCATGTTATTTCTCCcg                            <  1:511163/140‑1 (MQ=255)
                                                                                                              cTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCATGTTATTTCTCCcg                            >  2:366113/1‑140 (MQ=255)
                                                                                                              cTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCATGTTATTTCTCCcg                            >  2:71859/1‑140 (MQ=255)
                                                                                                               ttCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCATGTTATTTCTCCcgt                           <  1:988576/140‑1 (MQ=255)
                                                                                                               ttCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCATGTTATTTCTCCcgt                           >  2:561833/1‑140 (MQ=255)
                                                                                                               ttCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCATGTTATTTCTCCcgt                           >  2:637183/1‑140 (MQ=255)
                                                                                                                       cgcgTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCATGTTATTTCTCCCgttgcgttgc                   >  1:670750/1‑140 (MQ=255)
                                                                                                                       cgcgTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCATGTTATTTCTCCCgttgcgttgc                   <  2:890475/140‑1 (MQ=255)
                                                                                                                       cgcgTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCATGTTATTTCTCCCgttgcgttgc                   >  2:304872/1‑140 (MQ=255)
                                                                                                                         cgTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCATGTTATTTCTCCCGTTGCGTTGCAt                 >  1:77016/1‑140 (MQ=255)
                                                                                                                         cgTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCATGTTATTTCTCCCGTTGCGTTGCAt                 >  2:871845/1‑140 (MQ=255)
                                                                                                                         cgTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCATGTTATTTCTCCCGTTGCGTTGCAt                 >  1:862685/1‑140 (MQ=255)
                                                                                                                            tGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCATGTTATTTCTCCCGTTGCGTTGCATTGt              >  1:273064/1‑140 (MQ=255)
                                                                                                                               ggATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCATGTTATTTCTCCCGTTGCGTTGCATTGTTTc           <  1:106930/140‑1 (MQ=255)
                                                                                                                                 aTCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCATGTTATTTCTCCCGTTGCGTTGCATTGTTTCAt         <  1:366113/140‑1 (MQ=255)
                                                                                                                                      ttttAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCATGTTATTTCTCCCGTTGCGTTGCATTGTTTCATTAata    <  1:623354/140‑1 (MQ=255)
                                                                                                                                        ttAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCATGTTATTTCTCCCGTTGCGTTGCATTGTTTCATTAATATg  <  1:851739/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                          
CATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCACGGTCTTCGGCGAAATTTCCTGAACCACCACGATGTTCGGCCATGTTATTTCTCCCGTTGCGTTGCATTGTTTCATTAATATG  >  NC_000913/1315799‑1316074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑