Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4350CT36.7% 35.1 / 16.9 30intergenic (‑179/‑28)ttdR/ttdADNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (1/18);  new base T (11/0);  total (12/18)
Fisher's exact test for biased strand distribution p-value = 2.20e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAA  >  NC_000913/3206296‑3206574
                                                                                                                                           |                                                                                                                                           
gCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTc                                                                                                                                             <  2:802655/140‑1 (MQ=255)
  aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGTCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCa                                                                                                                                           <  2:694810/140‑1 (MQ=255)
  aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCa                                                                                                                                           >  2:367854/1‑140 (MQ=255)
    aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGa                                                                                                                                         >  2:606491/1‑140 (MQ=255)
           ccATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAc                                                                                                                                  <  1:443375/140‑1 (MQ=255)
           ccATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAc                                                                                                                                  <  2:308286/140‑1 (MQ=255)
           acagtCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAc                                                                                                                                  <  1:486915/136‑1 (MQ=255)
                cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACaaaaa                                                                                                                             <  1:359992/140‑1 (MQ=255)
                 ccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAAc                                                                                                                            <  1:491187/140‑1 (MQ=255)
                   tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAaaaaaaaaaaaaaaaag                                                                                                                          >  2:359992/1‑137 (MQ=255)
                   tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATg                                                                                                                          >  1:507467/1‑140 (MQ=255)
                     ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGa                                                                                                                        <  1:292838/140‑1 (MQ=255)
                     ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAAATTTTTCATAcaaaaacaaaaaaatgg                                                                                                                        >  2:437372/1‑135 (MQ=255)
                      tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                                       <  2:829878/140‑1 (MQ=255)
                      tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                                       <  2:467469/140‑1 (MQ=255)
                          gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTATACAAAAAAAAAAAATTGAGATgt                                                                                                                   >  2:486915/1‑139 (MQ=255)
                          gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAATGGAGGTGc                                                                                                                   >  1:909904/1‑140 (MQ=255)
                               atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAAAAAAAAATAGAGGTGCCctga                                                                                                              >  1:710371/1‑140 (MQ=255)
                               atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCGAAATTTTTCAGACAAAAAAAAAAACAGGGGGTGCCacga                                                                                                              >  2:834677/1‑140 (MQ=255)
                                      ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                       <  1:888782/140‑1 (MQ=255)
                                          tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAATTTTTTAAAAAAAAAAAAAAAATGGGGTTGGCATGATGAGCGAAAga                                                                                                   >  1:595569/1‑139 (MQ=255)
                                           aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGAGTTTCCATGATGAGGGAAAGta                                                                                                  >  1:105546/1‑140 (MQ=255)
                                           aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGta                                                                                                  <  2:167809/140‑1 (MQ=255)
                                                 gcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCACACAAAAAAAAAAAAACGAGTTGCCCTAATGAGCGAAAAtaaaaaaa                                                                                            >  1:325356/1‑138 (MQ=255)
                                                 gcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAAAAAAAAAAGGAGTTGGCATGATTAGCGAAAGaaaaaaaa                                                                                            >  2:126032/1‑138 (MQ=255)
                                                       aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                                      <  1:469369/140‑1 (MQ=255)
                                                              aaCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGaa                                                                               <  1:518279/140‑1 (MQ=255)
                                                                 tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGAGTTGCCATGATGAGCGAAAGTAATAAACAACAGGCAGTGAaaaa                                                                            >  1:709671/1‑140 (MQ=255)
                                                                     aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCCACAAAAACAAAAAATGGAGGTGCCATGATGAGCGAAAATAAAAAACAAAAAGCAGTGAaaaaaatg                                                                        >  1:933493/1‑136 (MQ=255)
                                                                           atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTAAAAAAAAAAAAAAAATGGAGGTGCCATTATGAGGGAAAAAAAAAAAAAAAAAACAGGGAATAAATTGACagag                                                                  >  2:580295/1‑140 (MQ=255)
                                                                            tatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAgaga                                                                 <  1:606491/140‑1 (MQ=255)
                                                                                    gTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGGGGTGCCATGATGAGCGAAAAAAAAAAACAAAAAGCAGTGAAAAAAATGACAGAGATTGTCGCt                                                         >  2:8722/1‑140 (MQ=255)
                                                                                                ataAAACCACATTTTTGGTGGTAGTTTGTGGGGGTGATTTTTTCCAGACAAATACAAAAACGGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGcc                                             <  1:437372/140‑1 (MQ=255)
                                                                                                 aaaacCCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCa                                            <  2:709671/139‑1 (MQ=255)
                                                                                                     aCCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGAt                                        <  1:810153/140‑1 (MQ=255)
                                                                                                                   ggTAGTTTGTGGGGGTGAATTTTTCCAGAAAAATAAAAAAACGGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGc                          <  2:759145/140‑1 (MQ=255)
                                                                                                                        tttGTGGGGGTGATTTTTTCAAGAAAAATAAAAAAACTGGAGTTCCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGTTTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCtgat                     <  2:710371/140‑1 (MQ=255)
                                                                                                                              gCGGTGAATTTTTTCAAAAAAAAACAAAAAAAGGAGGTGGCCAGATGAGCGAAAGGAATAAACAAAAAACAGAGAATAAATTTACAGAGATTGTCGCTAACTTTACCCCCCCGATTTCTTACCGAAAGCCTGATTACgtg               >  2:425384/1‑140 (MQ=255)
                                                                                                                                 gTGAATTTTTTCAGACAAAAACAAAAAATGGAGGTGCCATGATGAGCGGAAATAATAAACAACAAGCAAGGAAAAAAATGACAGAGAATGTTGCTCACTTTACCCCCATGATTTCTACCCGAATGCCTGATGACgcgggg            >  2:7688/1‑138 (MQ=255)
                                                                                                                                      tttttCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaa       <  1:8722/140‑1 (MQ=255)
                                                                                                                                           ccAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa  <  1:280679/140‑1 (MQ=255)
                                                                                                                                           ccAAACAAATACAAAAACTGGATTTGCCATGATGAGCGAAAGAAATAAGCAACAGGAAGTGAATAAGTTGACATAGATTGCCGCTAACTTAACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa  <  2:850806/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
GCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAA  >  NC_000913/3206296‑3206574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑