Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,429,9840TG24.4% 78.2 / 11.0 41L41V (TTG→GTG) cycAserine/alanine/glycine/cycloserine:H(+)symporter
Reads supporting (aligned to +/- strand):  ref base T (7/24);  new base G (10/0);  total (17/24)
Fisher's exact test for biased strand distribution p-value = 1.73e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCA‑TTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAA  >  NC_000913/4429847‑4430117
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tACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCGTTGGCACCGGggtg                                                                                                                                      >  1:915638/1‑140 (MQ=255)
tACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGgttg                                                                                                                                      <  2:104500/140‑1 (MQ=255)
tACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGgttg                                                                                                                                      >  1:918900/1‑140 (MQ=255)
     gaagaaAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGGTGTTTAt                                                                                                                                 >  1:774092/1‑140 (MQ=255)
       agaaAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATgg                                                                                                                               >  1:379329/1‑140 (MQ=255)
                 aTGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCaa                                                                                                                     <  2:753072/140‑1 (MQ=255)
                 aTGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCaa                                                                                                                     <  2:442118/140‑1 (MQ=255)
                      aGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGa                                                                                                                <  1:185727/140‑1 (MQ=255)
                       gATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGAt                                                                                                               <  2:968746/140‑1 (MQ=255)
                        aTCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGAtt                                                                                                              <  2:379329/140‑1 (MQ=255)
                           aGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGc                                                                                                           >  1:636146/1‑140 (MQ=255)
                               aaaaGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTAAGGGGGTGTTTTTTGGGTCTGGCAAAACGATTAGCCttt                                                                                                       >  2:303805/1‑139 (MQ=255)
                                 aaGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGcc                                                                                                     <  2:1005766/140‑1 (MQ=255)
                                       ttGCCGATTATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGGTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCg                                                                                               >  2:831729/1‑140 (MQ=255)
                                       ttGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACg                                                                                                                 <  1:463833/122‑1 (MQ=255)
                                       ttGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACg                                                                                                                 >  2:463833/1‑122 (MQ=255)
                                       ttGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCg                                                                                               >  1:34611/1‑140 (MQ=255)
                                       ttGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGGACGGGGGTGTTTATGGGGGCTGGCAAAACGATTAGCCTTGCCGGGGCg                                                                                               >  1:621986/1‑140 (MQ=255)
                                                atCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCAtt                                                                                      <  1:341652/140‑1 (MQ=255)
                                                 tCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCAttt                                                                                     <  1:727328/140‑1 (MQ=255)
                                                 tCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCAttt                                                                                     <  2:246611/140‑1 (MQ=255)
                                                  cAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCAtttt                                                                                    <  1:909115/140‑1 (MQ=255)
                                                     gCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGt                                                                                 <  1:432461/140‑1 (MQ=255)
                                                      cTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGtt                                                                                <  1:494453/140‑1 (MQ=255)
                                                       tCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCCTTGGGACGGGGGTGTTTTTTGGGGCTGGCAAAACGATTAGCCTTGCCGGGGCGTCGGTCATTTTTGttt                                                                               >  2:313041/1‑140 (MQ=255)
                                                       tCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGttt                                                                               <  1:783684/140‑1 (MQ=255)
                                                         cGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTtat                                                                             <  1:497199/140‑1 (MQ=255)
                                                          ggCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCCTTGGGACGGGGGTGTTTATTGGGGCTGGCAAAAAGATTAGCCTTGCCGGGGCGGCGATCATTTTTGTTtatt                                                                            >  2:779598/1‑139 (MQ=255)
                                                          ggCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTtata                                                                            <  1:933785/140‑1 (MQ=255)
                                                          ggCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGGACGGGGGTGTTTTTGGGGTCTGGCAAAACGATTAGCCTTGCCGGGGCGTCGATCATTTTCGTTtata                                                                            >  1:258583/1‑140 (MQ=255)
                                                           gCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCCTTGGTACGGGGGTGTTTTTGGGGGCTGGCAAAACGATTAGCCTTGCCGGGCCGGCGATCATTTTTGTTTatat                                                                           >  2:933785/1‑140 (MQ=255)
                                                           gCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTatat                                                                           <  1:831729/140‑1 (MQ=255)
                                                               aaCAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGGCATTGGTACGGGGGTGTTTATGGGGGCTGGCAAAACGATTAGCCTTGCCGGGGCGGCGATCATTTTTGTTTTtattatt                                                                       >  2:368215/1‑139 (MQ=255)
                                                                      gCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCA‑TTGGtt                                                               <  2:918900/140‑1 (MQ=255)
                                                                         aCGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACAGGGGTTTTTTTGGGGTCTGGCAAAAAGATTAGCCCTTCCGGGCCCGCGGTCATTTTTGTTTTTATGATCATTTGGtttt                                                             >  2:876840/1‑140 (MQ=255)
                                                                             cgcAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCA‑TTGGTTTTATGct                                                        <  1:450996/140‑1 (MQ=255)
                                                                                            cGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCA‑TTGGTTTTATGCTCTTTTTCGTGATGCg                                         <  1:28688/140‑1 (MQ=255)
                                                                                                          tATTGCCATTGGCGGTGCCATTGGTACGGGGGTGTTTTTGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCA‑TTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAAt                           >  1:380904/1‑140 (MQ=255)
                                                                                                           aTTGCCATTGGCGGTGCCATTGGTACGGGGGTGTTTTTTGGGGCTGGCAAAACGATTAGCCTTGCCGGGCCGGCGATCATTTTTTTTTATATTATCA‑TTGGTTTTATGCTCTTTTTTGTGATGCGGGGAATGGGGGGAtt                          >  2:431422/1‑140 (MQ=255)
                                                                                                            ttGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCA‑TTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATtg                         <  2:636146/140‑1 (MQ=255)
                                                                                                            ttGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCA‑TTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATtg                         <  1:313041/140‑1 (MQ=255)
                                                                                                            ttGCCATTGGCGGTGCCATTGGTACGGGGGTGTTTATTGGGTCTGGCAAAACGATTAGCCTTTCCGGGCCGTCGATCATTTTTGTTTATATGATCA‑TTTGTTTTATGCTCTTTTTCGTGATGCGGGGAATGGGGGGAttt                         >  2:362864/1‑139 (MQ=255)
                                                                                                             tGCCATTGGCGGTGCCATTGGTACGGGGGTGTTTTTGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTTGTTTATATGATCA‑TTGGTTTTTTGCTCTTTTTTGTGATGCGGGCAATGGGGGGATtgt                        >  2:157996/1‑139 (MQ=255)
                                                                                                                  ttGGCGGTGCCATTGGGACGGGGGTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGGCGATCATTTTCGTTTATATGATCA‑TTGGGTTTATGCTCTTTTTTGTGATGCGGGCAATGGGGGGATTGCTGCtt                   >  1:123613/1‑140 (MQ=255)
                                                                                                                              ttGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCA‑TTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGaa       <  2:34611/140‑1 (MQ=255)
                                                                                                                                   aCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCA‑TTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACaa  >  2:569874/1‑140 (MQ=255)
                                                                                                                                   aCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCA‑TTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACaa  >  2:737222/1‑140 (MQ=255)
                                                                                                                                   aCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCA‑TTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACaa  <  1:779598/140‑1 (MQ=255)
                                                                                                                                   aCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCA‑TTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACaa  >  1:820802/1‑140 (MQ=255)
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TACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCA‑TTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAA  >  NC_000913/4429847‑4430117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑