Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,490,314	G→A	A546V (GCA→GTA)	oxc ←	oxalyl‑CoA decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,490,314	0	G	A	100.0%	169.8 / NA	50	A546V (GCA→GTA)	oxc	oxalyl‑CoA decarboxylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/27);  total (23/27)

TTAGCATAATAGTATTTCCGATAAGGGTCCCCTCACCATCGCATAATGAGTTGGCAAGAGGAGACGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATAC  >  NC_000913/2490180‑2490448                                                                                                                                       |                                                                                                                                       ttAGCATAATAGTATTTCCGATAAGGGTCCCCTCACCATCGCATAATGAGTTGGCAAGAGGAGACGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTCCctgcagta                                                                                                                                   <  1:76816/140‑5 (MQ=255)      ataataGTATTTCCGATAAGGGTCCCCTCACCATCGCATAATGAGTTGGCAAGAGGAGACGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTc                                                                                                                              >  1:871267/1‑140 (MQ=255)         ataGTATTTCCGATAAGGGTCCCCTCACCATCGCATAATGAGTTGGCAAGAGGAGACGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGAt                                                                                                                           <  2:575136/140‑1 (MQ=255)          taGTATTTCCGATAAGGGTCCCCTCACCATCGCATAATGAGTTGGCAAGAGGAGACGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATg                                                                                                                          >  1:474890/1‑140 (MQ=255)            gTATTTCCGATAAGGGTCCCCTCACCATCGCATAATGAGTTGGCAAGAGGAGACGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGAc                                                                                                                        <  2:606212/140‑1 (MQ=255)            gTATTTCCGATAAGGGTCCCCTCACCATCGCATAATGAGTTGGCAAGAGGAGACGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGAc                                                                                                                        <  2:423874/140‑1 (MQ=255)            gTATTTCCGATAAGGGTCCCCTCACCATCGCATAATGAGTTGGCAAGAGGAGACGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGAc                                                                                                                        <  2:464201/140‑1 (MQ=255)            gTATTTCCGATAAGGGTCCCCTCACCATCGCATAATGAGTTGGCAAGAGGAGACGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGAc                                                                                                                        <  1:496050/140‑1 (MQ=255)            atttttCCGATAAGGGGTCCCTCTCCATCGCATAATGAGTTGGCAAGAGGAGACGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGAc                                                                                                                        <  2:158730/137‑1 (MQ=255)             tATTTCCGATAAGGGTCCCCTCACCATCGCATAATGAGTTGGCAAGAGGAGACGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGAcc                                                                                                                       <  1:166432/140‑1 (MQ=255)               tttCCGATAAGGGTCCCCTCACCATCGCATAATGAGTTGGCAAGAGGAGACGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACcac                                                                                                                     >  2:27356/1‑140 (MQ=255)               tttCCGATAAGGGTCCCCTCACCATCGCATAATGAGTTGGCAAGAGGAGACGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACcac                                                                                                                     >  1:55154/1‑140 (MQ=255)               tttCCGATAAGGGTCCCCTCACCATCGCATAATGAGTTGGCAAGAGGAGACGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACcac                                                                                                                     >  1:811791/1‑140 (MQ=255)                  ccGATAAGGGTCCCCTCACCATCGCATAATGAGTTGGCAAGAGGAGACGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACAtt                                                                                                                  >  2:647529/1‑140 (MQ=255)                     aTAAGGGTCCCCTCACCATCGCATAATGAGTTGGCAAGAGGAGACGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATtaat                                                                                                               >  1:596342/1‑140 (MQ=255)                       aaGGGTCCCCTCACCATCGCATAATGAGTTGGCAAGAGGAGACGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATtaataa                                                                                                             >  1:971899/1‑140 (MQ=255)                               ccTCACCATCGCATAATGAGTTGGCAAGAGGAGACGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGt                                                                                                     <  2:811791/140‑1 (MQ=255)                                  cACCATCGCATAATGAGTTGGCAAGAGGAGACGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTg                                                                                                  <  1:336687/140‑1 (MQ=255)                                   aCCATCGCATAATGAGTTGGCAAGAGGAGACGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTAGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTgc                                                                                                 >  2:339483/1‑140 (MQ=255)                                    ccATCGCATAATGAGTTGGCAAGAGGAGACGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTgcg                                                                                                <  1:647529/140‑1 (MQ=255)                                    ccATCGCATAATGAGTTGGCAAGAGGAGACGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTgcg                                                                                                <  1:670341/140‑1 (MQ=255)                                    ccATCGCATAATGAGTTGGCAAGAGGAGACGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTgcg                                                                                                <  1:419237/140‑1 (MQ=255)                                       tCGCATAATGAGTTGGCAAGAGGAGACGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGa                                                                                             <  2:55154/140‑1 (MQ=255)                                        cGCATAATGAGTTGGCAAGAGGAGACGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGAc                                                                                            >  1:526366/1‑140 (MQ=255)                                        cGCATAATGAGTTGGCAAGAGGAGACGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGAc                                                                                            <  1:134179/140‑1 (MQ=255)                                               tGAGTTGGCAAGAGGAGACGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATAc                                                                                     >  1:474177/1‑140 (MQ=255)                                                  gTTGGCAAGAGGAGACGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCgg                                                                                  >  2:118033/1‑140 (MQ=255)                                                     ggCAAGAGGAGACGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCggtgg                                                                               >  1:422340/1‑140 (MQ=255)                                                           aggagACGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTTAATACCGGTGGTTAAAg                                                                         <  1:321975/140‑1 (MQ=255)                                                                aCGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATg                                                                     <  2:1007591/139‑1 (MQ=255)                                                                aCGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATg                                                                     >  1:1007591/1‑139 (MQ=255)                                                                              tAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGt                                                      <  1:518903/140‑1 (MQ=255)                                                                                    ccAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCgtggt                                                >  2:706827/1‑140 (MQ=255)                                                                                    ccAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCgtggt                                                >  2:864776/1‑140 (MQ=255)                                                                                     cAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCgtggtg                                               <  2:971899/140‑1 (MQ=255)                                                                                     cAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCgtggtg                                               <  2:328360/140‑1 (MQ=255)                                                                                     cAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCgtggtg                                               <  1:706827/140‑1 (MQ=255)                                                                                     cAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCgtggtg                                               <  1:118033/140‑1 (MQ=255)                                                                                      aGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGa                                              >  2:344031/1‑140 (MQ=255)                                                                                                tttGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGcc                                    <  2:474177/140‑1 (MQ=255)                                                                                                                   tatGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATcc                 >  1:723584/1‑140 (MQ=255)                                                                                                                    atGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCa                >  1:193371/1‑140 (MQ=255)                                                                                                                     tGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCAt               >  2:108136/1‑140 (MQ=255)                                                                                                                       gCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTa             <  2:27885/140‑1 (MQ=255)                                                                                                                        ccACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTaa            <  1:864776/140‑1 (MQ=255)                                                                                                                        ccACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTaa            >  1:673582/1‑140 (MQ=255)                                                                                                                         cACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAAt           <  2:596342/140‑1 (MQ=255)                                                                                                                              tCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTc      <  2:673582/140‑1 (MQ=255)                                                                                                                                 gTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTc      >  2:390542/1‑137 (MQ=255)                                                                                                                                 gTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTc      <  1:390542/137‑1 (MQ=255)                                                                                                                                  ttCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATAc  >  2:835234/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                       TTAGCATAATAGTATTTCCGATAAGGGTCCCCTCACCATCGCATAATGAGTTGGCAAGAGGAGACGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATAC  >  NC_000913/2490180‑2490448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑