Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,494,424	T→C	intergenic (‑22/‑274)	yfdX ← / → ypdI	protein YfdX/colanic acid synthesis putative lipoprotein YpdI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,494,424	0	T	C	100.0%	298.8 / NA	79	intergenic (‑22/‑274)	yfdX/ypdI	protein YfdX/colanic acid synthesis putative lipoprotein YpdI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (49/30);  total (49/30)

TTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGTTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTACAATTGTAGGAATAT  >  NC_000913/2494289‑2494558                                                                                                                                        |                                                                                                                                       ttGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTa                                                                                                                                    >  2:671099/1‑140 (MQ=255)   gCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGa                                                                                                                                  >  1:102519/1‑140 (MQ=255)            gcttaGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAAcgccgc                                                                                                                         >  2:163534/5‑140 (MQ=255)             cTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACgccgcc                                                                                                                        <  2:215820/140‑1 (MQ=255)             cTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACgccgcc                                                                                                                        <  2:102519/140‑1 (MQ=255)              tGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCca                                                                                                                       >  1:986749/1‑140 (MQ=255)                  tttGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCcatcat                                                                                                                   >  2:990195/1‑140 (MQ=255)                  tttGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCcatcat                                                                                                                   >  1:604429/1‑140 (MQ=255)                     gCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATAtt                                                                                                                >  1:77032/1‑140 (MQ=255)                      cTGTTGTGCCGCTACCGGCGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTTATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTg                                                                                                               >  2:964963/1‑140 (MQ=255)                        gttgtGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCGCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTggg                                                                                                             <  2:284077/140‑1 (MQ=255)                         ttgtGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGt                                                                                                            <  1:98167/140‑1 (MQ=255)                            tgCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAg                                                                                                         >  1:289767/1‑140 (MQ=255)                                 cTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGa                                                                                                    <  1:214445/140‑1 (MQ=255)                                   aCCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGaaa                                                                                                  <  2:986749/140‑1 (MQ=255)                                       gAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATgag                                                                                              >  1:345433/1‑140 (MQ=255)                                           aTTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAAt                                                                                          >  2:525190/1‑140 (MQ=255)                                                  gcagcCCATACAGTTGAAGATACCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCagagag                                                                                   >  2:645613/1‑140 (MQ=255)                                                   cagcCCATACAGTTGAAGATGCCAGAATTGCTGTTACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGc                                                                                  >  2:100395/1‑140 (MQ=255)                                                    agcCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCa                                                                                 >  2:479797/1‑140 (MQ=255)                                                     gcCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAAt                                                                                          >  1:628822/1‑130 (MQ=255)                                                     gcCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAAt                                                                                          <  2:628822/130‑1 (MQ=255)                                                      cccATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCaa                                                                                >  2:405963/1‑139 (MQ=255)                                                      cccATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCaa                                                                                <  1:405963/139‑1 (MQ=255)                                                      cccATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAAc                                                                               <  1:1002609/140‑1 (MQ=255)                                                      cccATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAAc                                                                               >  1:505801/1‑140 (MQ=255)                                                         aTACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGt                                                                            >  2:903696/1‑140 (MQ=255)                                                                tGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTg                                                                     >  1:649907/1‑140 (MQ=255)                                                                tGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTg                                                                     >  2:786039/1‑140 (MQ=255)                                                                tGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTg                                                                     >  1:767747/1‑140 (MQ=255)                                                                  aaGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGaa                                                                   <  1:163534/140‑1 (MQ=255)                                                                  aaGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGaa                                                                   >  2:887493/1‑140 (MQ=255)                                                                    gATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAg                                                                 <  2:216145/140‑1 (MQ=255)                                                                      tGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAggg                                                               <  1:786039/140‑1 (MQ=255)                                                                      tGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAggg                                                               <  2:604429/140‑1 (MQ=255)                                                                      tGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAggg                                                               <  1:479797/140‑1 (MQ=255)                                                                      tGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAggg                                                               <  2:505801/140‑1 (MQ=255)                                                                       gCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAggg                                                               >  2:440288/1‑139 (MQ=255)                                                                       gCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAggg                                                               <  1:440288/139‑1 (MQ=255)                                                                         cAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTa                                                            >  2:904502/1‑140 (MQ=255)                                                                         cAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTa                                                            >  2:98032/1‑140 (MQ=255)                                                                                 cTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAg                                                    >  1:663141/1‑140 (MQ=255)                                                                                 cTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAg                                                    <  1:550967/140‑1 (MQ=255)                                                                                   gtgACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGtt                                                  >  2:41225/1‑140 (MQ=255)                                                                                   gtgACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGtt                                                  <  1:964963/140‑1 (MQ=255)                                                                                    tgACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACaactg                                                 <  1:993367/140‑5 (MQ=255)                                                                                      aCCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTa                                               >  2:330542/1‑140 (MQ=255)                                                                                      aCCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTa                                               >  1:671597/1‑140 (MQ=255)                                                                                         aTCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTAGtt                                            >  1:761287/1‑140 (MQ=255)                                                                                         aTCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTAGtt                                            >  2:957195/1‑140 (MQ=255)                                                                                           cGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTAGttat                                          >  2:552975/1‑140 (MQ=255)                                                                                           cGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTAGttat                                          >  2:917701/1‑140 (MQ=255)                                                                                               gCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTAGTTATTATc                                      >  2:799473/1‑140 (MQ=255)                                                                                                    aaTTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCaag                                 <  1:957195/140‑1 (MQ=255)                                                                                                    aaTTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCaag                                 <  1:41225/140‑1 (MQ=255)                                                                                                      ttAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCaagaa                               <  1:671099/140‑1 (MQ=255)                                                                                                            gTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTAttt                         <  1:243760/140‑1 (MQ=255)                                                                                                            gTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTAttt                         <  1:525190/140‑1 (MQ=255)                                                                                                              ttCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTa                       >  2:83486/1‑140 (MQ=255)                                                                                                                cATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCaagaa                               <  1:707177/130‑1 (MQ=255)                                                                                                                cATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCaagaa                               >  2:707177/1‑130 (MQ=255)                                                                                                                cATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGt                     >  2:430755/1‑140 (MQ=255)                                                                                                                cATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGt                     >  1:51270/1‑140 (MQ=255)                                                                                                                 aTACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTc                    >  2:325902/1‑140 (MQ=255)                                                                                                                    cTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTa                 <  1:799473/140‑1 (MQ=255)                                                                                                                     ttACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTAc                <  2:663141/140‑1 (MQ=255)                                                                                                                       aCCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTACaa              >  2:895325/1‑140 (MQ=255)                                                                                                                       aCCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTACaa              <  1:917701/140‑1 (MQ=255)                                                                                                                       aCCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTACaa              >  1:33323/1‑140 (MQ=255)                                                                                                                           cATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTa                 >  1:941282/1‑133 (MQ=255)                                                                                                                           cATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTa                 <  2:941282/133‑1 (MQ=255)                                                                                                                            aTTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAg                      <  2:18741/127‑1 (MQ=255)                                                                                                                            aTTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAg                      >  1:18741/1‑127 (MQ=255)                                                                                                                            aTTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTACAATTGTa         >  2:690099/1‑140 (MQ=255)                                                                                                                            aTTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTACAATTGTa         >  2:947051/1‑140 (MQ=255)                                                                                                                             ttCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTACAATTGTAg        >  2:581885/1‑140 (MQ=255)                                                                                                                                 ttAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTACAATTGTAGGAat    >  2:485497/1‑140 (MQ=255)                                                                                                                                  tAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTACAATTGTAGGAata   >  1:299348/1‑140 (MQ=255)                                                                                                                                   aaCAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTACAATTGTAGGAatat  >  2:646878/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                       TTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGTTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTACAATTGTAGGAATAT  >  NC_000913/2494289‑2494558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑