Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	435,813	T→A	D60E (GAT→GAA)	thiL →	thiamine monophosphate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	435,813	0	T	A	100.0%	247.8 / NA	72	D60E (GAT→GAA)	thiL	thiamine monophosphate kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (35/37);  total (35/37)

GTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGATCCTGCTGATCTGGCTTATAAAGCACTGGCGGTGAACCTAAGCGATCTGGCAGCGATGGGGGCCGATCCGGCCTGGCTGACGCTGGCATTAACCTTACCGGACGTAGACGAAGCGTGGCTTGAGTCCTTCAGCGAC  >  NC_000913/435677‑435948                                                                                                                                         |                                                                                                                                        gtgtAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACct                                                                                                                                      >  1:1002159/1‑140 (MQ=255) gtgtAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACct                                                                                                                                      >  2:861648/1‑140 (MQ=255)  tgtAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACctg                                                                                                                                     >  1:100488/1‑140 (MQ=255)  tgtAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACctg                                                                                                                                     <  1:815955/140‑1 (MQ=255)  tgtAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACctg                                                                                                                                     >  2:781092/1‑140 (MQ=255)  tgtAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACctg                                                                                                                                     <  1:201339/140‑1 (MQ=255)  tgtAAGAAGTTCTCGTCTTGATGTCGAACTGGACATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACctg                                                                                                                                     <  2:36570/140‑1 (MQ=255)   gtAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACctgc                                                                                                                                    >  2:753994/1‑140 (MQ=255)   gtAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACctgc                                                                                                                                    >  1:645659/1‑140 (MQ=255)       gaagTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACCTGCTGAt                                                                                                                                <  1:671573/140‑1 (MQ=255)       gaagTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACCTGCTGAt                                                                                                                                <  1:231887/140‑1 (MQ=255)          gTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACCTGCTGATCTg                                                                                                                             <  2:235078/140‑1 (MQ=255)          gTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACCTGCTGATCTg                                                                                                                             <  1:494843/140‑1 (MQ=255)            tctcGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACCTGCTGATCTGGc                                                                                                                           >  2:350038/1‑140 (MQ=255)            tctcGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACCTGCTGATCTGGc                                                                                                                           <  1:350038/140‑1 (MQ=255)            tctcGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCACTTCCTCCCTGATATCGAACCTGCTGATCTGGc                                                                                                                           <  2:226469/140‑1 (MQ=255)             ctcGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACctg                                                                                                                                     >  1:652211/1‑129 (MQ=255)             ctcGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACctg                                                                                                                                     <  2:652211/129‑1 (MQ=255)                gTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACCTGCTGAt                                                                                                                                >  1:458947/1‑131 (MQ=255)                gTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACCTGCTGAt                                                                                                                                <  2:458947/131‑1 (MQ=255)                    tGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACCTGCTGATCTGGCTTATAAAg                                                                                                                   >  2:230068/1‑140 (MQ=255)                       tGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACCTGCTGATCTGGCTTATAAAGCAc                                                                                                                <  2:100488/140‑1 (MQ=255)                        gTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACCTGCTGATCTGGCTTATAAAGCACt                                                                                                               <  2:287593/140‑1 (MQ=255)                            aaCTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACCTGCTGATCTGGCTTATAAAGCACTGGCg                                                                                                           <  2:607803/140‑1 (MQ=255)                              cTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACCTGCTGATCTGGCTTATAAAGCACTGGCGGt                                                                                                         <  1:419185/140‑1 (MQ=255)                              cTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACCTGCTGATCTGGCTTATAAAGCACTGGCGGt                                                                                                         <  2:645659/140‑1 (MQ=255)                               tGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACCTGCTGATCTGGCTTATAAAGCACTGGCGGTg                                                                                                        <  2:1002159/140‑1 (MQ=255)                                 ggCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACCTGCTGATCTGGCTTATAAAGCACTGGCGGt                                                                                                         >  1:722746/1‑137 (MQ=255)                                 ggCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACCTGCTGATCTGGCTTATAAAGCACTGGCGGt                                                                                                         <  2:722746/137‑1 (MQ=255)                                 ggCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACCTGCTGATCTGGCTTATAAAGCACTGGCGGTGaa                                                                                                      >  2:206851/1‑140 (MQ=255)                                      cGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACCTGCTGATCTGGCTTATAAAGCACTGGCGGTGAACCTaa                                                                                                 >  2:698735/1‑140 (MQ=255)                                      cGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACCTGCTGATCTGGCTTATAAAGCACTGGCGGTGAACCTaa                                                                                                 >  1:932178/1‑140 (MQ=255)                                           acgaTTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGGAACCATTTCCTCCCTGATATCGAACCTGCTGATCTGGCTTATAAAGCACTCGCGGTGAACCCaacgaat                                                                                            >  2:244835/1‑135 (MQ=255)                                                 gcgcACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACCTGCTGATCTGGCTTATAAAGCACTGGCGGTGAACCTAAGCGATCTGGCa                                                                                      <  1:119835/140‑1 (MQ=255)                                                         ctcAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACCTGCTGATCTGGCTTATAAAGCACTGGCGGTGAACCTAAGCGATCTGGCAGCGATggg                                                                              >  1:998983/1‑140 (MQ=255)                                                            aaTATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACCTGCTGATCTGGCTTATAAAGCACTGGCGGTGAACCTAAGCGATCTGGCAGCGATGGGGGc                                                                           <  2:932178/140‑1 (MQ=255)                                                                 ccccGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACCTGCTGATCTGGCTTATAAAGCACTGGCGGTGAACCTAAGCGATCTGGCAGCGATGGGGGCCGATc                                                                      >  1:908799/1‑140 (MQ=255)                                                                 ccccGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACCTGCTGATCTGGCTTATAAAGCACTGGCGGTGAACCTAAGCGATCTGGCAGCGATGGGGGCCGATc                                                                      >  1:697709/1‑140 (MQ=255)                                                                 ccccGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACCTGCTGATCTGGCTTATAAAGCACTGGCGGTGAACCTAAGCGATCTGGCAGCGATGGGGGCCGATc                                                                      >  1:656520/1‑140 (MQ=255)                                                                 ccccGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACCTGCTGATCTGGCTTATAAAGCACTGGCGGGGAACCTAAGCGATCTTGCAGCGATGGGGGGCGATc                                                                      >  2:30043/1‑140 (MQ=255)                                                                     gagaAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACCTGCTGATCTGGCTTATAAAGCACTGGCGGTGAACCTAAGCGATCTGGCAGCGATGGGGGCCGATCCGGc                                                                  <  1:781092/140‑1 (MQ=255)                                                                          aCAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACCTGCTGATCTGGCTTATAAAGCACTGGCGGTGAACCTAAGCGATCTGGCAGCGATGGGGGCCGATCCGGCCTGGc                                                             <  2:441871/140‑1 (MQ=255)                                                                              aCCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACCTGCTGATCTGGCTTATAAAGCACTGGCGGTGGACCCAAGAGATCTTGCAGCGATGGGGGGCGATCCCGCCCGGGTGAc                                                         >  2:375706/1‑140 (MQ=255)                                                                                  tGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACCTGCTGATCTGGCTTATAAAGCACTGGCGGGGAACCCAAACGATCTGGCAGCGCTGGGGGGCGGTCCGGCCCGGCTGACGCTg                                                     >  2:974061/1‑140 (MQ=255)                                                                                      gATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACCTGCTGATCTGGCTTATAAAGCACTGGCGGTGAACCTAAGCGATCTGGCAGCGATGGGGGCCGATCCGGCCTGGCTGACGCTGGCAt                                                 <  1:118152/140‑1 (MQ=255)                                                                                          aGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACCTGCTGATCTGGCTTATAAAGCACTGGCGGTGGACCCAAACGATCTTGCAGCGATGGGGGGCGATCCCGCCTGGCTGACGCTGGGATTaaa                                             >  2:737975/1‑139 (MQ=255)                                                                                          aGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACCTGCTGATCTGGCTTATAAAGCACTGGCGGTGAAACTAAGCGATCTGGCAGCGATGGGGGGCGATCCGGCCTGGCTGACGCTGGCAtttaa                                             >  2:622923/1‑137 (MQ=255)                                                                                           gCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACCTGCTGATCTGGCTTATAAAGCACTGGCGGTGAACCTAAGCGATCTGGCAGCGATGGGGGCCGATCCGGCCTGGCTGACGCTGGCATTAAcc                                            <  2:166997/140‑1 (MQ=255)                                                                                            cACTGATACGCTGGTGGCGGGGAACCATTTCCTCCCTGATATCGAACCTGCTGATCTGGCTTATAAAGCACTGGCGGTGAACCTAAGCGCACTGGCAACGATGGGGGGCGATGCGGCCTGGGTGACGCCGGCATTaaacc                                           >  2:916274/1‑137 (MQ=255)                                                                                               tGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACCTGCTGATCTGGCTTATAAAGCACTGGCGGTGAACCTAAGCGATCTGGCAGCGATGGGGGCCGATCCGGCCTGGCTGACGCTGGCATTAACCTTAc                                        <  1:737975/140‑1 (MQ=255)                                                                                                 aTACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACCTGCTGATCTGGCTTATAAAGCACTGGCGGTGAACCTAAGCGATCTGGCAGCGATGGGGGCCGATCCGGCCTGGCTGACGc                                                       <  2:142172/123‑1 (MQ=255)                                                                                                 aTACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACCTGCTGATCTGGCTTATAAAGCACTGGCGGTGAACCTAAGCGATCTGGCAGCGATGGGGGCCGATCCGGCCTGGCTGACGc                                                       >  1:142172/1‑123 (MQ=255)                                                                                                 aTACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACCTGCTGATCTGGCTTATAAAGCACTGGCGGTGAACCTAAGCGATCTGGCAGCGATGGGGGCCGATCCGGCCTGGCTGACGCTGGCATTAACCTTACCg                                      <  1:119648/140‑1 (MQ=255)                                                                                                 aTACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACCTGCTGATCTGGCTTATAAAGCACTGGCGGTGAACCTAAGCGATCTGGCAGCGATGGGGGCCGATCCGGCCTGGCTGACGCTGGCATTAACCTTACCg                                      <  1:206851/140‑1 (MQ=255)                                                                                                 aTACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACCTGCTGATCTGGCTTATAAAGCACTGGCGGTGAACCTAAGCGATCTGGCAGCGATGGGGGCCGATCCGGCCTGGCTGACGCTGGCATTAACCTTACCg                                      <  1:30043/140‑1 (MQ=255)                                                                                                    cGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAACCTGCTGATCTGGCTTATAAAGCACTGGCGGTGAACCTAAGCGATCTGGCAGCGATGGGGGCCGATCCGGCCTGGCTGACGCTGGCATTAACCTTACCGGAc                                   >  2:278801/1‑140 (MQ=255)                                                                                                         gtggCGGGTAACCATTTCCTCCCTGATATCGAACCTGCTGATCTGGCTTATAAAGCACTGACGGTGAACCTAAGCGATCTGGCAGCGATGGGGGCCGATCCGGCCTGGCTGACGCTGGCATTAACCTTACCGGACGTAGa                              >  1:968438/1‑140 (MQ=255)                                                                                                          tggCGGGTAACCATTTCCTCCCTGATATCGAACCTGCTGATCTGGCTTATAAAGCACTGGCGGTGAACCTAAGCGATCTGGCAGCGATGGGGGCCGATCCGGCCTGGCTGACGCTGGCATTAACCTTACCGGACGTAGAc                             <  2:656520/140‑1 (MQ=255)                                                                                                           ggCGGGTAACCATTTCCTCCCTGATATCGAACCTGCTGATCTGGCTTATAAAGCACTGGCGGTGAACCTAAGCGATCTGGCAGCGATGGGGGCCGATCCGGCCTGGCTGACGCTGGCATTAACCTTACCGGACGTAGACg                            >  1:189727/1‑140 (MQ=255)                                                                                                           ggCGGGTAACCATTTCCTCCCTGATATCGAACCTGCTGATCTGGCTTATAAAGCACTGGCGGTGAACCTAAACGATCTTGCAGCGATGGGGGCCGATCCCGCCTGGCTGACGCTGGCATTAAACTTACCGGACGGAGACg                            >  2:70715/1‑140 (MQ=255)                                                                                                                    ccATTTCCTCCCTGATATCGAACCTGCTGATCTGGCTTATAAAGCACTGGCGGTGAACCTAAGCGATCTGGCAGCGATGGGGGCCGATCCGGCCTGGCTGACGCTGGCATTAACCTTACCGGACGTAGACGAAGCGTGGc                   <  1:70715/140‑1 (MQ=255)                                                                                                                    ccATTTCCTCCCTGATATCGAACCTGCTGATCTGGCTTATAAAGCACTGGCGGTGAACCTAAGCGATCTGACAGCGATGGGGGCCGATCCGGCCTGGCTGACGCTGGCATTAACCTTACCGGACGTAGACGAAGCGTGGc                   <  1:977697/140‑1 (MQ=255)                                                                                                                      aTTTCCTCCCTGATATCGAACCTGCTGATCTGGCTTATAAAGCACTGGCGGTGAACCTAAGCGATCTGGCAGCGATGGGGGCCGATCCGGCCTGGCTGACGCTGGCATTAACCTTACCGGACGTAGACGAAGCGTGGCtt                 <  1:244835/140‑1 (MQ=255)                                                                                                                      aTTTCCTCCCTGATATCGAACCTGCTGATCTGGCTTATAAAGCACTGGCGGTGAACCTAAGCGATCTGGCAGCGATGGGGGCCGATCCGGCCTGGCTGACGCTGGCATTAACCTTACCGGACGTAGACGAAGCGTGGCtt                 <  1:852728/140‑1 (MQ=255)                                                                                                                       tttCCTCCCTGATATTGAACCTGCTGATCTGGCTTATAAAGCACTGGCGGTGAACCTAAGCGATCTGGCAGCGATGGGGGCCGATCCGGCCTGGCTGACGCTGGCATTAACCTTACCGGACGTAGACGAAGCGTGGCTTg                <  2:606101/140‑1 (MQ=255)                                                                                                                       tttCCTCCCTGATATCGAACCTGCTGATCTGGCTTATAAAGCACTGGCGGTGAACCTAAGCGATCTGGCAGCGATGGGGGCCGATCCGGCCTGGCTGACGCTGGCATTAACCTTACCGGACGTAGACGAAGCGTGGCTTg                <  2:27053/140‑1 (MQ=255)                                                                                                                        ttCCTCCCTGATATCGAACCTGCTGATCTGGCTTATAAAGCACTGGCGGTGAACCTAAGCGATCTGGCAGCGATGGGGGCCGATCCGGCCTGGCTGACGCTGGCATTAACCTTACCGGACGTAGACGAAGCGTGGCTTGa               >  1:251774/1‑140 (MQ=255)                                                                                                                              ccTGATATCGAACCTGCTGATCTGGCTTATAAAGCACTGGCGGTGAACCTAAGCGATCTGGCAGCGATGGGGGCCGATCCGGCCTGGCTGACGCTGGCATTAACCTTACCGGACGTAGACGAAGCGTGGCTTGAGTCCtt         >  1:348154/1‑140 (MQ=255)                                                                                                                              ccTGATATCGAACCTGCTGATCTGGCTTATAAAGCACTGGCGGTGAACCTAAGCGATCTGGCAGCGATGGGGGCCGATCCGGCCTGGCTGACGCTGGCATTAACCTTACCGGACGTAGACGAAGCGTGGCTTGAGTCCtt         >  1:916469/1‑140 (MQ=255)                                                                                                                                    atCGAACCTGCTGATCTGGCTTATAAAGCACTGGCGGTGAACCTAAGCGATCTGGCAGCGATGGGGGCCGATCCGGCCTGGCTGACGCTGGCATTAACCTTACCGGACGTAGACGAAGCGTGGCTTGAGTCCTTCAGCGa   >  1:618918/1‑140 (MQ=255)                                                                                                                                    atCGAACCTGCTGATCTGGCTTATAAAGCACTGGCGGTGAACCTAAGCGATCTGGCAGCGATGGGGGCCGATCCGGCCTGGCTGACGCTGGCATTAACCTTACCGGACGTAGACGAAGCGTGGCTTGAGTCCTTCAGCGa   <  1:861648/140‑1 (MQ=255)                                                                                                                                     tCGAACCTGCTGATCTGGCTTATAAAGCACTGGCGGTGAACCTAAGCGATCTGGCAGCGATGGGGGCCGATCCGGCCTGGCTGACGCTGGCATTAACCTTACCGGACGTAGACGAAGCGTGGCTTGAGTCCTTCAGCGAc  >  1:76978/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        GTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGATCCTGCTGATCTGGCTTATAAAGCACTGGCGGTGAACCTAAGCGATCTGGCAGCGATGGGGGCCGATCCGGCCTGGCTGACGCTGGCATTAACCTTACCGGACGTAGACGAAGCGTGGCTTGAGTCCTTCAGCGAC  >  NC_000913/435677‑435948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑