| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,696,638 | +AT | coding (838/1557 nt) | mltF → | membrane‑bound lytic murein transglycosylase F |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,696,636 | 1 | . | A | 92.9% | 136.0 / 3.1 | 42 | coding (836/1557 nt) | mltF | membrane‑bound lytic murein transglycosylase F |
| Reads supporting (aligned to +/- strand): ref base . (3/0); new base A (20/19); total (23/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.39e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01 | |||||||||||
| * | NC_000913 | 2,696,636 | 2 | . | T | 92.9% | 135.8 / 3.1 | 42 | coding (836/1557 nt) | mltF | membrane‑bound lytic murein transglycosylase F |
| Reads supporting (aligned to +/- strand): ref base . (3/0); new base T (20/19); total (23/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.39e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.36e-01 | |||||||||||
TTAGATGGCGATAATACCCTTTCCGCCGCCCTGCTCGACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCAC‑‑ATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATGCACAG > NC_000913/2696503‑2696760 || ttAGATGGCGATAATACCCTTTCCGCCGCCCTGCTCGACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCAC‑‑Atattt > 2:870570/1‑140 (MQ=255) tGGCGATAATACCCTTTCCGCCGCCCTGCTCGACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCAC‑‑Atatttttacg > 1:869162/1‑135 (MQ=255) tCCGCCGCCCTGCTCGACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATATTTTTACGCGCCGTCGATGCGGTa > 2:525145/1‑140 (MQ=255) tCCGCCGCCCTGCTCGACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATATTTTTACGCGCCGTCGATGCGGTa < 2:215094/140‑1 (MQ=255) ccgccCTGCTCGACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATATTTTTACGCGCCGTCGATGCGGTACTGc > 1:867516/1‑140 (MQ=255) cTCGACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTa < 1:414674/140‑1 (MQ=255) aCTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGc < 2:867516/140‑1 (MQ=255) tcttcAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGcccc < 2:869162/140‑1 (MQ=255) atgaatgaAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATATTTTTACGCGCTGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGaaa > 2:945301/1‑140 (MQ=255) atgaatgaAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGaaa < 1:525145/140‑1 (MQ=255) tgaatgaaGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAAt > 1:382160/1‑140 (MQ=255) tgaatgaaGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATATTTTTACGCGCCGCCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTg > 1:356434/1‑134 (MQ=255) tgaatgaaGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATATTTTTACGCGCCGCCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTg < 2:356434/134‑1 (MQ=255) aCGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCgaag > 2:869626/1‑140 (MQ=255) cGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCgaaga > 2:751996/1‑140 (MQ=255) ggTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCgaagaa > 2:211953/1‑140 (MQ=255) gTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGaaa > 2:451304/1‑140 (MQ=255) gTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGaaa > 2:424554/1‑140 (MQ=255) gCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGa < 1:211953/140‑1 (MQ=255) ggCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCga > 2:92352/1‑128 (MQ=255) ggCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCga < 1:92352/128‑1 (MQ=255) cACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCAC‑‑ATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGt > 1:10511/1‑140 (MQ=255) aTTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTg < 2:928456/140‑1 (MQ=255) aTTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTg < 2:48214/140‑1 (MQ=255) aaGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTgg < 1:424554/140‑1 (MQ=255) aaTACCTGGGGCATGGCGATGATTTTGTTTACGTCGATACGCGCACATATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGc > 2:791271/1‑135 (MQ=255) aaTACCTGGGGCATGGCGATGATTTTGTTTACGTCGATACGCGCACATATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGc < 1:791271/135‑1 (MQ=255) ccTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGc < 1:960851/140‑1 (MQ=255) ggggCATGGCGATGATTTTGATTACGTCGATACGCGCACATATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTa > 2:777360/1‑140 (MQ=255) ggCATGGCGATGATTTTGATTACGTCGATACGCGCACATATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTAt > 1:57329/1‑132 (MQ=255) ggCATGGCGATGATTTTGATTACGTCGATACGCGCACATATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTAt < 2:57329/132‑1 (MQ=255) ggCATGGCGATGATTTTGATTACGTCGATACGCGCACATATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAAAAATTGACTGGCGTCTGCTGGACGCTATTGCTTATc > 1:374120/1‑140 (MQ=255) aTGGCGATGATTTTGATTACGTCGATACGCGCACATATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAgg < 1:751996/140‑1 (MQ=255) tGGCGATGATTTTGATTACGTCGATACGCGCACATATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGa > 2:606629/1‑140 (MQ=255) tGGCGATGATTTTGATTACGTCGATACGCGCACATATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGa > 2:716104/1‑140 (MQ=255) tGGCGATGATTTTGATTACGTCGATACGCGCACATATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGa > 2:576216/1‑140 (MQ=255) ggCGATGATTTTGATTACGTCGATACGCGCACATATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGaa > 1:730595/1‑140 (MQ=255) ggCGATGATTTTGATTACGTCGATACGCGCACATATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGaa < 1:696779/140‑1 (MQ=255) tgatTTTGATTACGTCGATACGCGCACATATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCa < 1:870570/140‑1 (MQ=255) tgatTTTGATTACGTCGATACGCGCACATATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCa < 2:382160/140‑1 (MQ=255) cGTCGATACGCGCACATATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATGcaca < 1:777360/140‑1 (MQ=255) gTCGATACGCGCACATATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATGCACAg > 1:21565/1‑140 (MQ=255) || TTAGATGGCGATAATACCCTTTCCGCCGCCCTGCTCGACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCAC‑‑ATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATGCACAG > NC_000913/2696503‑2696760 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |