Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	716,842	Δ10 bp	intergenic (‑245/+95)	ybfG ← / ← potE	PF10964 domain‑containing protein YbfG/putrescine:H(+) symporter/putrescine:ornithine antiporter PotE

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	716842	716851	10	69 [0]	[0] 65	ybfG/potE	PF10964 domain‑containing protein YbfG/putrescine:H(+) symporter/putrescine:ornithine antiporter PotE

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 716841	0 (0.000)	65 (1.120)	43/276	0.2	100%	intergenic (‑244/+105)	ybfG/potE	PF10964 domain‑containing protein YbfG/putrescine:H(+) symporter/putrescine:ornithine antiporter PotE
	?	NC_000913	716852 =	0 (0.000)					intergenic (‑255/+94)	ybfG/potE	PF10964 domain‑containing protein YbfG/putrescine:H(+) symporter/putrescine:ornithine antiporter PotE

TTTTTCAAAGCATAAACCGATACCCAATACCGGTTTCAGTAATAAAATGGCGAGGCCGGGCCGGATCCTGTTCCAGTTTTTGTCGCAGATGCCCCATATAATTACGCAAATAGTTGCTGTGTTCGATCGCGTTTAGACCCTACATCTGCCGGATGCGGCATAAATAACGAACTATTTGCCAACAAACAGAAAGGGCGATCATTCAATCGCCCTTTTTTACTTTGTTTTATTAACCGTGTTTATTTTTCAGTTCAAAGCGTGGTGATACCAGAC  >  NC_000913/716717‑716989                                                                                                                                        |                                                                                                                                          cattgccacttttttcaaagcataaaccgatacccaataccggtttcagtaataaaatggcgaggccgggccggatcctgttccagtttttgtcgcagatgccccatataattacgcaaatagttgctgtgttcgGacct                                                                                                                                       >  2:843807‑M2/136‑139 (MQ=255) cattgccacttttttcaaagcataaaccgatacccaataccggtttcagtaataaaatggcgaggccgggccggatcctgttccagtttttgtcgcagatgccccatataattacgcaaatagttgctgtgttcgGAccc                                                                                                                                       >  1:111804‑M2/136‑140 (MQ=255)    tgccacttttttcaaagcataaaccgatacccaataccggtttcagtaataaaatggcgaggccgggccggatcctgttccagtttttgtcgcagatgccccatataattacgcaaatagttgctgtgttcgGACCCTAc                                                                                                                                    >  1:196152‑M2/133‑140 (MQ=255)    tgccacttttttcaaagcataaaccgatacccaataccggtttcagtaataaaatggcgaggccgggccggatcctgttccagtttttgtcgcagatgccccatataattacgcaaatagttgctgtgttcgGACCCTAc                                                                                                                                    >  1:913173‑M2/133‑140 (MQ=255)    tgccacttttttcaaagcataaaccgatacccaataccggtttcagtaataaaatggcgaggccgggccggatcctgttccagtttttgtcgcagatgccccatataattacgcaaatagatgctgtgttcgGACCCTAc                                                                                                                                    >  1:976125‑M2/133‑140 (MQ=255)      ccacttttttcaaagcataaaccgatacccaataccggtttcagtaataaaatggcgaggccgggccggatcctgttccagtttttgtcgcagatgccccatataattacgcaaatagttgctgtgttcgGACCCTACAt                                                                                                                                  >  2:662620‑M2/131‑140 (MQ=255)            ttttcaaagcataaaccgatacccaataccggtttcagtaataaaatggcgaggccgggccggatcctgttccagtttttgtcgcagatgccccatataattacgcaaatagttgctgtgttcgGACCCTACATCTGCCg                                                                                                                            >  1:928154‑M2/125‑140 (MQ=255)               tcaaagcataaaccgatacccaataccggtttcagtaataaaatggcgaggccgggccggatcctgttccagtttttgtcgcagatgccccatataattacgcaaatagttgctgtgttcgGACCCTACATCTGCCGGAt                                                                                                                         <  2:111804‑M2/19‑1 (MQ=255)                  aagcataaaccgatacccaataccggtttcagtaataaaatggcgaggccgggccggatcctgttccagtttttgtcgcagatgccccatataattacgcaaatagttgctgtgttcgGACCTTACATCTGCCGGATGCg                                                                                                                      <  1:843807‑M2/22‑1 (MQ=255)                              atacccaataccggtttcagtaataaaatggcgaggccgggccggatcctgttccagtttttgtcgcagatgccccatataattacgcaaatagttgctgtgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACg                                                                                                          <  1:108959‑M2/34‑1 (MQ=255)                               tacccaataccggtttcagtaataaaatggcgaggccgggccggatcctgttccagtttttgtcgcagatgccccatataattacgcaaatagttgctgtgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACg                                                                                                          >  1:736777‑M2/106‑139 (MQ=255)                               tacccaataccggtttcagtaataaaatggcgaggccgggccggatcctgttccagtttttgtcgcagatgccccatataattacgcaaatagttgctgtgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACg                                                                                                          <  2:736777‑M2/34‑1 (MQ=255)                               tacccaataccggtttcagtaataaaatggcgaggccgggccggatcctgttccagtttttgtcgcagatgccccatataattacgcaaatagttgctgtgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACGa                                                                                                         >  1:142405‑M2/106‑140 (MQ=255)                               tacccaataccggtttcagtaataaaatggcgaggccgggccggatcctgttccagtttttgtcgcagatgccccatataattacgcaaatagttgctgtgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACGa                                                                                                         >  2:31263‑M2/106‑140 (MQ=255)                                    aataccggtttcagtaataaaatggcgaggccgggccggatcctgttccagtttttgtcgcagatgccccatataattacgcaaatagttgctgtgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACg                                                                                                          >  1:860738‑M2/101‑134 (MQ=255)                                    aataccggtttcagtaataaaatggcgaggccgggccggatcctgttccagtttttgtcgcagatgccccatataattacgcaaatagttgctgtgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACg                                                                                                          <  2:860738‑M2/34‑1 (MQ=255)                                         cggtttcagtaataaaatggcgaggccgggccggatcctgttccagtttttgtcgcagatgccccatataattacgcaaatagttgctgtgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACGAACTATTTGcc                                                                                               <  1:554484‑M2/45‑1 (MQ=255)                                           gtttcagtaataaaatggcgaggccgggccggatcctgttccagtttttgtcgcagatgccccatataattacgcaaatagttgctgtgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACGAACTATTTGCcaa                                                                                             >  1:32600‑M2/94‑140 (MQ=255)                                           gtttcagtaataaaatggcgaggccgggccggatcctgttccagtttttgtcgcagatgccccatataattacgcaaatagttgctgtgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACGAACTATTTGCcaa                                                                                             >  2:119325‑M2/94‑140 (MQ=255)                                           gtttcagtaataaaatggcgaggccgggccggatcctgttccagtttttgtcgcagatgccccatataattacgcaaatagttgctgtgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACGAACTATTTGCcaa                                                                                             >  1:437330‑M2/94‑140 (MQ=255)                                           gtttcagtaataaaatggcgaggccgggccggatcctgttccagtttttgtcgcagatgccccatataattacgcaaatagttgctgtgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACGAACTATTTGCcaa                                                                                             >  2:435439‑M2/94‑140 (MQ=255)                                            tttcagtaataaaatggcgaggccgggccggatcctgttccagtttttgtcgcagatgccccatataattacgcaaatagttgctgtgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACGAACTATTTGCcaac                                                                                            >  1:778917‑M2/93‑140 (MQ=255)                                            tttcagtaataaaatggcgaggccgggccggatcctgttccagtttttgtcgcagatgccccatataattacgcaaatagttgctgtgttcgGACCCCACATCTGCCGGATGCGGCATAAATAACGAACTATTTGCcaac                                                                                            >  1:63619‑M2/93‑140 (MQ=255)                                                agtaataaaatggcgaggccgggccggatcctgttccagtttttgtcgcagatgccccatataattacgcaaatagttgctgtgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACGAACTATTTGCCaacaaac                                                                                        >  2:894062‑M2/89‑140 (MQ=255)                                                agtaataaaatggcgaggccgggccggatcctgttccagtttttgtcgcagatgccccatataattacgcaaatagttgctgtgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACGAACTATTTGCCaacaaac                                                                                        >  1:223445‑M2/89‑140 (MQ=255)                                                 gtaataaaatggcgaggccgggccggatcctgttccagtttttgtcgcagatgccccatataattacgcaaatagttgctgtgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACGAACTATTTGCCaacaaaca                                                                                       <  1:894062‑M2/53‑1 (MQ=255)                                                         atggcgaggccgggccggatcctgttccagtttttgtcgcagatgccccatataattacgcaaatagttgctgtgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACGAACTATTTGCCAACAAACAGAAAGGGc                                                                               <  2:606067‑M2/61‑1 (MQ=255)                                                         atggcgaggccgggccggatcctgttccagtttttgtcgcagatgccccatataattacgcaaatagttgctgtgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACGAACTATTTGCCAACAAACAGAAAGGGc                                                                               <  1:598461‑M2/61‑1 (MQ=255)                                                            gcgaggccgggccggatcctgttccagtttttgtcgcagatgccccatataattacgcaaatagttgctgtgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACGAACTATTTGCCAACAAACAGAAAGGGc                                                                               <  1:505581‑M2/61‑1 (MQ=255)                                                            gcgaggccgggccggatcctgttccagtttttgtcgcagatgccccatataattacgcaaatagttgctgtgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACGAACTATTTGCCAACAAACAGAAAGGGc                                                                               >  2:505581‑M2/77‑137 (MQ=255)                                                            gcgaggccgggccggatcctgttccagtttttgtcgcagatgccccatataattacgcaaatagttgctgtgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACGAACTATTTGCCAACAAACAGAAAGGGCGAt                                                                            >  1:286761‑M2/77‑140 (MQ=255)                                                               aggccgggccggatcctgttccagtttttgtcgcagatgccccatataattacgcaaatagttgctgtgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACGAACTATTTGCCAACAAACAGAAAGGGCGATCAt                                                                         >  1:466641‑M2/74‑140 (MQ=255)                                                                ggccgggccggctcctgttccagtttttgtcgcagatgccccatataattacgcaaatagttgctgtgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACGAACTATTTGCCAACAAACAGAAAGGGCGATCAtt                                                                        <  2:408000‑M2/68‑1 (MQ=255)                                                                      gccggatcctgttccagtttttgtcgcagatgccccatataattacgcaaatagttgctgtgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACGAACTATTTGCCAACAAACAGAAAGGGCGATCATTCAATCg                                                                  <  2:873020‑M2/74‑1 (MQ=255)                                                                      gccggatcctgttccagtttttgtcgcagatgccccatataattacgcaaatagttgctgtgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACGAACTATTTGCCAACAAACAGAAAGGGCGATCATTCAATCg                                                                  <  1:467855‑M2/74‑1 (MQ=255)                                                                                 ttccagtttttgtcgcagatgccccatataattacgcaaatagttgctgtgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACGAACTATTTGCCAACAAACAGAAAGGGCGATCATTCAATCGCCCTTTTTTAc                                                       <  2:300449‑M2/85‑1 (MQ=255)                                                                                  tccagtttttgtcgcagatgccccatataattacgcaaatagttgctgtgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACGAACTATTTGCCAACAAACAGAAAGGGCGATCATTCAATCGCCCTTTTTTACt                                                      <  2:384069‑M2/86‑1 (MQ=255)                                                                                  tccagtttttgtcgcagatgccccatataattacgcaaatagttgctgtgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACGAACTATTTGCCAACAAACAGAAAGGGCGATCATTCAATCGCCCTTTTTTACt                                                      <  2:928154‑M2/86‑1 (MQ=255)                                                                                    cagtttttgtcgcagatgccccatataattacgcaaatagttgctgtgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACGAACTATTTGCCAACAAACAGAAAGGGCGATCATTCAATCGCCCTTTTTTACttt                                                    >  2:875412‑M2/53‑140 (MQ=255)                                                                                    cagtttttgtcgcagatgccccatataattacgcaaatagttgctgtgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACGAACTATTTGCCAACAAACAGAAAGGGCGATCATTCAATCGCCCTTTTTTACttt                                                    <  2:830367‑M2/88‑1 (MQ=255)                                                                                    cagtttttgtcgcagatgccccatataattacgcaaatagttgctgtgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACGAACTATTTGCCAACAAACAGAAAGGGCGATCATTCAATCGCCCTTTTTTACttt                                                    <  2:688033‑M2/88‑1 (MQ=255)                                                                                      gtttttgtcgcagatgccccatataattacgcaaatagttgctgtgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACGAACTATTTGCCAACAAACAGAAAGGGCGATCATTCAATCGCCCTTTTTTACTTTGt                                                  >  1:356832‑M2/51‑140 (MQ=255)                                                                                           tgtcgcagatgccccatataattacgcaaatagttgctgtgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACGAACTATTTGCCAACAAACAGAAAGGGCGATCATTCAATCGCCCTTTTTTACTTTGTTttat                                             <  1:35558‑M2/95‑1 (MQ=255)                                                                                               gcagatgccccatataattacgcaaatagttgctgtgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACGAACTATTTGCCAACAAACAGAAAGGGCGATCATTCAATCGCCCTTTTTTACTTTGTTTTATTAAc                                         <  2:356832‑M2/99‑1 (MQ=255)                                                                                                cagatgccccatataattacgcaaatagttgctgtgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACGAACTATTTGCCAACAAACAGAAAGGGCGATCATTCAATCGCCCTTTTTTACTTTGTTTTATTAAcc                                        <  1:838802‑M2/100‑1 (MQ=255)                                                                                                  gatgccccatataattacgcaaatagttgctgtgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACGAACTATTTGCCAACAAACAGAAAGGGCGATCATTCAATCGCCCTTTTTTACTTTGTTTTATTAACCgt                                      <  1:435439‑M2/102‑1 (MQ=255)                                                                                                    tgccccatataattacgcaaatagttgctgtgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACGAACTATTTGCCAACAAACAGAAAGGGCGATCATTCAATCGCCCTTTTTTACTTTGTTTTATTAACCgtgt                                    <  1:875412‑M2/104‑1 (MQ=255)                                                                                                       cccatataattacgcaaatagttgctgtgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACGAACTATTTGCCAACAAACAGAAAGGGCGATCATTCAATCGCCCTTTTTTACTTTGTTTTATTAACCGTGTTTa                                 >  1:593447‑M2/34‑140 (MQ=255)                                                                                                            ataattacgcaaatagttgctgtgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACGAACTATTTGCCAACAAACAGAAAGGGCGATCATTCAATCGCCCTTTTTTACTTTGTTTTATTAACCGTGTTTAttttt                            <  2:223445‑M2/112‑1 (MQ=255)                                                                                                               attacgcaaatagttgctgtgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACGAACTATTTGCCAACAAACAGAAAGGGCGATCATTCAATCGCCCTTTTTTACTTTGTTTTATTAACCGTGTTTATTTTTCAg                         >  1:635669‑M2/26‑140 (MQ=255)                                                                                                                ttacgcaaatagttgctgtgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACGAACTATTTGCCAACAAACAGAAAGGGCGATCATTCAATCGCCCTTTTTTACTTTGTTTTATTAACCGTGTTTATTTTTCAGt                        >  1:561936‑M2/25‑140 (MQ=255)                                                                                                                   cgcaaatagttgctgtgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACGAACTATTTGCCAACAAACAGAAAGGGCGATCATTCAATCGCCCTTTTTTACTTTGTTTTATTAACCGTGTTTa                                 <  2:459521‑M2/107‑1 (MQ=255)                                                                                                                   cgcaaatagttgctgtgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACGAACTATTTGCCAACAAACAGAAAGGGCGATCATTCAATCGCCCTTTTTTACTTTGTTTTATTAACCGTGTTTa                                 >  1:459521‑M2/22‑128 (MQ=255)                                                                                                                     caaatagttgctgtgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACGAACTATTTGCCAACAAACAGAAAGGGCGATCATTCAATCGCCCTTTTTTACTTTGTTTTATTAACCGTGTTTATTTTTCAGTTCaaa                   >  2:738400‑M2/20‑140 (MQ=255)                                                                                                                       aatagttgctgtgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACGAACTATTTGCCAACAAACAGAAAGGGCGATCATTCAATCGCCCTTTTTTACTTTGTTTTATTAACCGTGTTTATTTTTCAGTTCAAAGc                 <  2:635669‑M2/123‑1 (MQ=255)                                                                                                                           gttgctgtgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACGAACTATTTGCCAACAAACAGAAAGGGCGATCATTCAATCGCCCTTTTTTACTTTGTTTTATTAACCGTGTTTATTTTTCAGTTCAAAGCgtgg             <  2:871761‑M2/127‑1 (MQ=255)                                                                                                                                tgtgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACGAACTATTTGCCAACAAACAGAAAGGGCGATCATTCAATCGCCCTTTTTTACTTTGTTTTATTAACCGTGTTTATTTTTCAGTTCAAAGCGTGGTGATa        <  2:32600‑M2/132‑1 (MQ=255)                                                                                                                                tgtgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACGAACTATTTGCCAACAAACAGAAAGGGCGATCATTCAATCGCCCTTTTTTACTTTGTTTTATTAACCGTGTTTATTTTTCAGTTCAAAGCGTGGTGATa        <  2:976125‑M2/132‑1 (MQ=255)                                                                                                                                  tgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACGAACTATTTGCCAACAAACAGAAAGGGCGATCATTCAATCGCCCTTTTTTACTTTGTTTTATTAACCGTGTTTATTTTTCAGTTCAAAGCGTGGTGATAcc      <  2:593447‑M2/134‑1 (MQ=255)                                                                                                                                  tgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACGAACTATTTGCCAACAAACAGAAAGGGCGATCATTCAATCGCCCTTTTTTACTTTGTTTTATTAACCGTGTTTATTTTTCAGTTCAAAGCGTGGTGATAcc      <  1:119325‑M2/134‑1 (MQ=255)                                                                                                                                  tgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACGAACTATTTGCCAACAAACAGAAAGGGCGATCATTCAATCGCCCTTTTTTACTTTGTTTTATTAACCGTGTTTATTTTTCAGTTCAAAGCGTGGTGATAcc      <  1:536254‑M2/134‑1 (MQ=255)                                                                                                                                  tgttcgGACCCTACATCTGCCGGATGCGGCATAAATAACGAACTATTTGCCAACAAACAGAAAGGGCGATCATTCAATCGCCCTTTTTTACTTTGTTTTATTAACCGTGTTTATTTTTCAGTTCAAAGCGTGGTGATAcc      >  1:358571‑M2/7‑140 (MQ=255)                                                                                                                                  tgttcgGACCCCACATCTGCCGGATGCGGCATAAATAACGAACTATTTGCCAACAAACAGAAAGGGCGATCATTCAATCGCCCTTTTTTACTTTGTTTTATTAACCGTGTTTATTTTTCAGTTCAAAGCGTGGTGATAcc      <  2:63619‑M2/134‑1 (MQ=255)                                                                                                                                    ttcgGACCCTACAACTGCCGGATGCGGCATAAATAACGATCTATTTGCCAACAAACAGAAAGGGCGATCATTCAATCGCCCTTTTTTACTTTGTTTTATTAACCGTGTTTATTTTTCAGTTCAAAGCGTGGTGATACCAg    >  2:919161‑M2/5‑140 (MQ=255)                                                                                                                                      cgGACCCTACATCTGCCGGATGCGGCATAAATAACGAACTATTTGCCAACAAACAGAAAGGGCGATCATTCAATCGCCCTTTTTTACTTTGTTTTATTAACCGTGTTTATTTTTCAGTTCAAAGCGTGGTGATACCAGAc  >  2:428001‑M2/3‑140 (MQ=255)                                                                                                                                        |                                                                                                                                          TTTTTCAAAGCATAAACCGATACCCAATACCGGTTTCAGTAATAAAATGGCGAGGCCGGGCCGGATCCTGTTCCAGTTTTTGTCGCAGATGCCCCATATAATTACGCAAATAGTTGCTGTGTTCGATCGCGTTTAGACCCTACATCTGCCGGATGCGGCATAAATAACGAACTATTTGCCAACAAACAGAAAGGGCGATCATTCAATCGCCCTTTTTTACTTTGTTTTATTAACCGTGTTTATTTTTCAGTTCAAAGCGTGGTGATACCAGAC  >  NC_000913/716717‑716989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑