Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	667,643	T→C	N192S (AAT→AGT)	mrdA ←	peptidoglycan DD‑transpeptidase MrdA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	667,643	0	T	C	100.0%	214.2 / NA	56	N192S (AAT→AGT)	mrdA	peptidoglycan DD‑transpeptidase MrdA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/26);  total (30/26)

CGAATAACACGCCCACGGTTGTTAACTTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCATTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAA  >  NC_000913/667508‑667762                                                                                                                                        |                                                                                                                        cGAATAACACGCCCACGGTTGTTAACTTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCactatt                                                                                                                     <  2:691305/140‑1 (MQ=255)     tAACACGCCCACGGTTGTTAACTTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAgg                                                                                                                 >  2:359229/1‑140 (MQ=255)          cGCCCACGGTTGTTAACTTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTc                                                                                                            <  1:891709/140‑1 (MQ=255)            cccACGGTTGTTAACTTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGa                                                                                                          <  1:281499/140‑1 (MQ=255)              cACGGTTGTTAACTTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACg                                                                                                        >  1:601066/1‑140 (MQ=255)                cGGTTGTTAACTTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTc                                                                                                      >  2:600875/1‑140 (MQ=255)                           ttCAACCTCTTCATAACCGGTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTCTTTATCGTTGa                                                                                           <  1:927157/140‑1 (MQ=255)                               aCCTCTTCATAACCGGTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTCTTTATCGTTGAtttt                                                                                       <  1:359229/140‑1 (MQ=255)                                ccTCTTCATAACCGGTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTc                                                                                      >  2:964421/1‑140 (MQ=255)                                       aTAACCGGTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACAt                                                                               <  1:600875/140‑1 (MQ=255)                                              gTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTCTTTATCGTTGAtttt                                                                                       <  1:167319/125‑1 (MQ=255)                                              gTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTCTTTATCGTTGAtttt                                                                                       >  2:603113/1‑125 (MQ=255)                                              gTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTCTTTATCGTTGAtttt                                                                                       <  1:603113/125‑1 (MQ=255)                                              gTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTCTTTATCGTTGAtttt                                                                                       >  2:167319/1‑125 (MQ=255)                                              gTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGAt                                                                        >  1:646607/1‑140 (MQ=255)                                              gTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGAt                                                                        >  2:398525/1‑140 (MQ=255)                                                      gTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTggg                                                                >  1:365103/1‑140 (MQ=255)                                                             acaTCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGc                                                         <  1:325899/140‑1 (MQ=255)                                                              caTCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGcc                                                        >  2:961494/1‑140 (MQ=255)                                                              caTCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGcc                                                        <  1:110388/140‑1 (MQ=255)                                                               aTCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCg                                                       <  2:980326/140‑1 (MQ=255)                                                                tCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGa                                                      <  2:344870/140‑1 (MQ=255)                                                                 cTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGaa                                                     >  1:492193/1‑140 (MQ=255)                                                                     aTAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGt                                                 <  1:964421/140‑1 (MQ=255)                                                                      tAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGATCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTa                                                >  1:840899/1‑140 (MQ=255)                                                                         tAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGt                                                 <  1:444432/136‑1 (MQ=255)                                                                         tAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGt                                                 >  2:444432/1‑136 (MQ=255)                                                                           aCGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGt                                                 <  2:118374/134‑1 (MQ=255)                                                                           aCGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGt                                                 >  1:118374/1‑134 (MQ=255)                                                                           aCGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTa                                                <  2:84090/135‑1 (MQ=255)                                                                           aCGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTa                                                >  1:84090/1‑135 (MQ=255)                                                                           aCGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGAt                                           >  2:194590/1‑140 (MQ=255)                                                                           aCGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGAt                                           >  2:434547/1‑140 (MQ=255)                                                                            cGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGt                                                 >  1:194251/1‑133 (MQ=255)                                                                            cGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGt                                                 <  2:194251/133‑1 (MQ=255)                                                                              cTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGt                                        <  2:601066/140‑1 (MQ=255)                                                                               tCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTa                                       <  2:492193/140‑1 (MQ=255)                                                                                   tGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAacga                                   >  1:723653/1‑140 (MQ=255)                                                                                   tGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAacga                                   <  2:723653/140‑1 (MQ=255)                                                                                   tGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAacga                                   >  1:220051/1‑140 (MQ=255)                                                                                   tGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAacga                                   >  1:308067/1‑140 (MQ=255)                                                                                   tGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAacga                                   <  1:361810/140‑1 (MQ=255)                                                                                         gCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTta                             <  1:491582/140‑1 (MQ=255)                                                                                             aCCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGc                         >  1:317185/1‑140 (MQ=255)                                                                                             aCCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGc                         >  2:673453/1‑140 (MQ=255)                                                                                             aCCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGc                         >  1:484065/1‑140 (MQ=255)                                                                                                    tCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAAc                  >  1:598913/1‑140 (MQ=255)                                                                                                            tGCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGacac          <  1:195805/140‑1 (MQ=255)                                                                                                            tGCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGacac          >  2:115623/1‑140 (MQ=255)                                                                                                            tGCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTCTTTATCGTTGATTTCCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGacac          >  1:489571/1‑140 (MQ=255)                                                                                                             gCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGa             >  2:191514/1‑136 (MQ=255)                                                                                                             gCCGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGa             <  1:191514/136‑1 (MQ=255)                                                                                                              ccGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACAccc        >  1:156083/1‑140 (MQ=255)                                                                                                               cGCATAGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCg       <  2:823856/140‑1 (MQ=255)                                                                                                                    aGTTGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGaaaa  <  2:156083/140‑1 (MQ=255)                                                                                                                      ttGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACt                 >  2:511415/1‑123 (MQ=255)                                                                                                                      ttGGCCAGTTTGCCGTCACTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACt                 <  1:511415/123‑1 (MQ=255)                                                                                                                                        |                                                                                                                        CGAATAACACGCCCACGGTTGTTAACTTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCATTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAA  >  NC_000913/667508‑667762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑