Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,371,175	A→T	L467* (TTG→TAG)	nanT ←	N‑acetylneuraminate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,371,175	0	A	T	100.0%	159.2 / NA	46	L467* (TTG→TAG)	nanT	N‑acetylneuraminate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (24/22);  total (24/22)

TTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCTGAACGCGAGAAGGCATATCCAGCCCAATCAGCAGGATCACCACGAACGTCAGACTGAACGAGAGCGATGCCAGCGCAGTACCCAGATCCAGACGTTGAGCGATCAA  >  NC_000913/3371042‑3371305                                                                                                                                      |                                                                                                                                   ttCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCTACGCtt                                                                                                                              >  2:831845/1‑140 (MQ=255)  tCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCTACGCTTc                                                                                                                             >  2:137483/1‑140 (MQ=255)    ccTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCTACGCTTCCg                                                                                                                           >  2:112070/1‑140 (MQ=255)    ccTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCTACGCTTCCg                                                                                                                           >  2:304091/1‑140 (MQ=255)      ttAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGACAGCGTCATGAGTACGCTACGCTTCCggg                                                                                                                         >  2:898274/1‑140 (MQ=255)        aGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCTACGCTTCCGGgcg                                                                                                                       >  1:711492/1‑140 (MQ=255)         gCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCTACGCTTCCGGgcgc                                                                                                                      <  2:427401/140‑1 (MQ=255)                          tAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCTACGCTTCCGGGCGCAACCAACGCTGAAcgcg                                                                                                     >  2:939262/1‑140 (MQ=255)                            gACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCTACGCTTCCGGGCGCAACCAACGCTGAACGCgag                                                                                                   <  1:112070/140‑1 (MQ=255)                            gACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCTACGCTTCCGGGCGCAACCAACGCTGAACGCgag                                                                                                   <  2:711492/140‑1 (MQ=255)                             aCCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCTACGCTTCCGGGCGCAACCAACGCTGAACGCgaga                                                                                                  >  1:385280/1‑140 (MQ=255)                                gggCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCTACGCTTCCGGGCGCAACCAACGCTGAACGCgag                                                                                                   >  2:217150/1‑136 (MQ=255)                                gggCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCTACGCTTCCGGGCGCAACCAACGCTGAACGCgag                                                                                                   <  1:217150/136‑1 (MQ=255)                                gggCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCTACGCTTCCGGGCGCAACCAACGCTGAACGCga                                                                                                    <  1:477213/135‑1 (MQ=255)                                gggCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCTACGCTTCCGGGCGCAACCAACGCTGAACGCga                                                                                                    >  2:477213/1‑135 (MQ=255)                                gggCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCTACGCTTCCGGGCGCAACCAACGCTGAACGCGAGAAgg                                                                                               >  1:97855/1‑140 (MQ=255)                                gggCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCTACGCTTCCGGGCGCAACCAACGCTGAACGCGAGAAgg                                                                                               >  2:374750/1‑140 (MQ=255)                                gggCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACAGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCTACGCTTCCGGGCGCAACCAACGCTGAACGCGAGAAgg                                                                                               <  2:23866/140‑1 (MQ=255)                                 ggCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCTACGCTTCCGGGCGCAACCAACGCTGAACGCGAGAAGGc                                                                                              <  1:939262/140‑1 (MQ=255)                                  gCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCTACGCTTCCGGGCGCAACCAACGCTGAACGCgag                                                                                                   <  2:709545/134‑1 (MQ=255)                                  gCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCTACGCTTCCGGGCGCAACCAACGCTGAACGCgag                                                                                                   >  1:709545/1‑134 (MQ=255)                                  gCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCTACGCTTCCGGGCGCAACCAACGCTGAACGCGAGAAGGCa                                                                                             >  1:28196/1‑140 (MQ=255)                                   cAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCTACGCTTCCGGGCGCAACCAACGCTGAACGCGAGAAGGCat                                                                                            >  2:453252/1‑140 (MQ=255)                                       aGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCTACGCTTCCGGGCGCAACCAACGCTGAACGCGAGAAGGCATATcc                                                                                        >  1:179112/1‑140 (MQ=255)                                           ttAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCTACGCTTCCGGGCGCAACCAACGCTGAACGCGAGAAGGCATATCCAGcc                                                                                    >  1:365223/1‑140 (MQ=255)                                             aaCTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCTACGCTTCCGGGCGCAACCAACGCTGAACGCGAGAAGGCATATCCAGCCCa                                                                                  <  2:28196/140‑1 (MQ=255)                                             aaCTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCTACGCTTCCGGGCGCAACCAACGCTGAACGCGAGAAGGCATATCCAGCCCa                                                                                  <  2:97855/140‑1 (MQ=255)                                             aaCTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGACAGCGTCATGAGTACGCTACGCTTCCGGGCGCAACCAACGCTGAACGCGAGAAGGCATATCCAGCCCa                                                                                  <  1:898274/140‑1 (MQ=255)                                                ttttGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCTACGCTTCCGGGCGCAACCAACGCTGAACGCGAGAAGGCATATCCAGCCCAATc                                                                               <  2:179112/140‑1 (MQ=255)                                                              aaaTCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCTACGCTTCCGGGCGCAACCAACGCTGAACGCGAGAAGGCATATCCAGCCCAATCAGCAGGATcaccac                                                                 <  1:137483/140‑1 (MQ=255)                                                              aaaTCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCTACGCTTCCGGGCGCAACCAACGCTGAACGCGAGAAGGCATATCCAGCCCAATCAGCAGGATcaccac                                                                 <  1:831845/140‑1 (MQ=255)                                                               aaTCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCTACGCTTCCGGGCGCAACCAACGCTGAACGCGAGAAGGCATATCCAGCCCAATCAGCAGGATCACCACg                                                                <  2:605656/140‑1 (MQ=255)                                                                         ggCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCTACGCTTCCGGGCGCAACCAACGCTGAACGCGAGAAGGCATATCCAGCCCAATCAGCAGGATCACCACGAACGTCAGAc                                                      >  2:187694/1‑140 (MQ=255)                                                                                    acggcacggcACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCTACGCTTCCGGGCGCAACCAACGCTGAACGCGAGAAGGCATATCCAGCCCAATCAGCAGGATCACCACGAACGTCAGACTGAACGAGAGc                                           <  1:584134/140‑1 (MQ=255)                                                                                              acCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCTACGCTTCCGGGCGCAACCAACGCTGAACGCGAGAAGGCATATCCAGCCCAATCAGCAGGATCACCACGAACGTCAGACTGAACGAGAGCGATGCCAgcg                                 <  1:988591/140‑1 (MQ=255)                                                                                              acCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCTACGCTTCCGGGCGCAACCAACGCTGAACGCGAGAAGGCATATCCAGCCCAATCAGCAGGATCACCACGAACGTCAGACTGAACGAGAGCGATGCCAgcg                                 <  1:374750/140‑1 (MQ=255)                                                                                               ccGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCTACGCTTCCGGGCGCAACCAACGCTGAACGCGAGAAGGCATATCCAGCCCAATCAGCAGGATCACCACGAACGTCAGACTGAACGAGAGCGATGCCAgcgc                                <  1:441024/140‑1 (MQ=255)                                                                                                cGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCTACGCTTCCGGGCGCAACCAACGCTGAACGCGAGAAGGCATATCCAGCCCAATCAGCAGGATCACCACGAACGTCAGACTGAACGAGAGCGATGCCAGCGCa                               <  2:365223/140‑1 (MQ=255)                                                                                                cGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCTACGCTTCCGGGCGCAACCAACGCTGAACGCGAGAAGGCATATCCAGCCCAATCAGCAGGATCACCACGAACGTCAGACTGAACGAGAGCGATGCCAGCGCa                               >  1:572353/1‑140 (MQ=255)                                                                                                          tttACCGTCGATAGCGTCATGAGTACGCTACGCTTCCGGGCGCAACCAACGCTGAACGCGAGAAGGCATATCCAGCCCAATCAGCAGGATCACCACGAACGTCAGACTGAACGAGAGCGATGCCAGCGCAGTACCCAGAt                     >  1:981456/1‑140 (MQ=255)                                                                                                               cGTCGATAGCGTCATGAGTACGCTACGCTTCCGGGCGCAACCAACGCTGAACGCGAGAAGGCATATCCAGCCCAATCAGCAGGATCACCACGAACGTCAGACTGAACGAGAGCGATGCCAGCGCAGTACCCAGATCCAGa                >  1:926343/1‑140 (MQ=255)                                                                                                                 tCGATAGCGTCATGAGTACGCTACGCTTCCGGGCGCAACCAACGCTGAACGCGAGAAGGCATATCCAGCCCAATCAGCAGGATCACCACGAACGTCAGACTGAACGAGAGCGATGCCAGCGCAGTACCCAGATCCAGACg              >  1:171285/1‑140 (MQ=255)                                                                                                                   gATAGCGTCATGAGTACGCTACGCTTCCGGGCGCAACCAACGCTGAACGCGAGAAGGCATATCCAGCCCAATCAGCAGGATCACCACGAACGTCAGACTGAACGAGAGCGATGCCAGCGCAGTACCCAGATCCAGACGtt            <  1:453252/140‑1 (MQ=255)                                                                                                                      aGCGTCATGAGTACGCTACGCTTCCGGGCGCAACCAACGCTGAACGCGAGAAGGCATATCCAGCCCAATCAGCAGGATCACCACGAACGTCAGACTGAACGAGAGCGATGCCAGCGCAGTACCCAGATCCAGACGTTGAg         >  1:393454/1‑140 (MQ=255)                                                                                                                             tGAGTACGCTACGCTTCCGGGCGCAACCAACGCTGAACGCGAGAAGGCATATCCAGCCCAATCAGCAGGATCACCACGAACGTCAGACTGAACGAGAGCGATGCCAGCGCAGTACCCAGATCCAGACGTTGAGCGATCaa  <  2:171285/140‑1 (MQ=255)                                                                                                                             tGAGTACGCTACGCTTCCGGGCGCAACCAACGCTGAACGCGAGAAGGCATATCCAGCCAAATCAGCAGGATCACCACGAACGTCAGACTGAACGAGAGCGATGCCAGCGCAGTACCCAGATCCAGACGTTGAGCGATCaa  >  1:917391/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                   TTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCTGAACGCGAGAAGGCATATCCAGCCCAATCAGCAGGATCACCACGAACGTCAGACTGAACGAGAGCGATGCCAGCGCAGTACCCAGATCCAGACGTTGAGCGATCAA  >  NC_000913/3371042‑3371305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑