Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	684,941	C→T	V105V (GTG→GTA)	gltL ←	glutamate/aspartate ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	684,941	0	C	T	100.0%	189.6 / NA	56	V105V (GTG→GTA)	gltL	glutamate/aspartate ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/26);  total (30/26)

CGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACC  >  NC_000913/684806‑685072                                                                                                                                        |                                                                                                                                    cgcgcgAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAGAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATTGCGTTGAAGTACtt                                                                                                                                 >  1:528520/1‑140 (MQ=255) cgcgcgAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGTACtt                                                                                                                                 >  2:243685/1‑140 (MQ=255) cgcgcgAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGTACtt                                                                                                                                 >  1:625009/1‑140 (MQ=255) cgcgcgAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGTACtt                                                                                                                                 >  1:736003/1‑140 (MQ=255) cgcgcgAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGTACtt                                                                                                                                 >  1:454829/1‑140 (MQ=255) cgcgcgAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGTACtt                                                                                                                                 >  2:269620/1‑140 (MQ=255) cgcgcgAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGTACtt                                                                                                                                 >  2:40924/1‑140 (MQ=255) cgcgcgAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGTACtt                                                                                                                                 >  2:739514/1‑140 (MQ=255) cgcgcgAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGTACtt                                                                                                                                 >  1:154470/1‑140 (MQ=255) cgcgcgAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGTACtt                                                                                                                                 >  2:970082/1‑140 (MQ=255)  gcgcgAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGTACttt                                                                                                                                >  2:222266/1‑140 (MQ=255)   cgcgAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGTACTTTc                                                                                                                               >  1:881468/1‑140 (MQ=255)     cgagcgaTTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGTACTTTCAc                                                                                                                             >  2:439902/1‑140 (MQ=255)     cgagcgaTTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGTACTTTCAc                                                                                                                             >  2:810262/1‑140 (MQ=255)           aTTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGTACTTTCACCTgcgc                                                                                                                       <  2:422665/140‑1 (MQ=255)               ccACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGTACTTTCACCTGCGCCAgg                                                                                                                   <  1:439902/140‑1 (MQ=255)               ccACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGTACTTTCACCTGCGCCAgg                                                                                                                   <  1:810262/140‑1 (MQ=255)                          tgctgACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGTACTTTCACCTgcg                                                                                                                        <  1:153591/124‑1 (MQ=255)                          tgctgACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGTACTTTCACCTgcg                                                                                                                        >  2:153591/1‑124 (MQ=255)                            ctgACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGTACTTTCACCTGCGCCAGGGTCAGGTTTTCGa                                                                                                      <  1:364659/140‑1 (MQ=255)                             tgACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGTACTTTCACCTGCGCCAGGGTCAGGTTTTCGAt                                                                                                     >  2:536096/1‑140 (MQ=255)                              gACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGTACTTTCACCTGCGCCAGGGTCAGGTTTTCGATa                                                                                                    <  2:625009/140‑1 (MQ=255)                               aCCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGTACTTTCACCTGCGCCAGGGTCAGGTTTTCGATaa                                                                                                   >  1:127489/1‑140 (MQ=255)                               aCCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGTACTTTCACCTGCGCCAGGGTCAGGTTTTCGATaa                                                                                                   >  2:173227/1‑140 (MQ=255)                                 cgccgGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGTACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATc                                                                                                 >  2:431121/1‑140 (MQ=255)                                  gccgGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGTACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCg                                                                                                <  1:643039/140‑1 (MQ=255)                                  gccgGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGTACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCg                                                                                                <  1:536096/140‑1 (MQ=255)                                   ccgGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGTACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGa                                                                                               <  1:892105/140‑1 (MQ=255)                                   ccgGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGTACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGa                                                                                               <  2:881468/140‑1 (MQ=255)                                   ccgGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGTACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGa                                                                                               <  2:102502/140‑1 (MQ=255)                                   ccgGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGTACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGa                                                                                               <  2:650291/140‑1 (MQ=255)                                    cgGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGTACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGAc                                                                                              >  1:376972/1‑140 (MQ=255)                                     ggAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGTACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACa                                                                                             <  1:222266/140‑1 (MQ=255)                                      gAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGTACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAg                                                                                            <  2:478838/140‑1 (MQ=255)                                       aaaGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGTACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGa                                                                                           <  1:738058/140‑1 (MQ=255)                                               gcCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGTACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGaa                                                                                   <  1:838275/140‑1 (MQ=255)                                                       cTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGTACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGa                                                                           <  2:454829/140‑1 (MQ=255)                                                            tGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGTACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGc                                                                      <  1:269620/140‑1 (MQ=255)                                                                  gAGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGTACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAAt                                                                >  1:611786/1‑140 (MQ=255)                                                                  gAGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGTACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAAt                                                                <  2:127489/140‑1 (MQ=255)                                                                   aGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGTACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATa                                                               >  1:258294/1‑140 (MQ=255)                                                                   aGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGTACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATa                                                               >  1:509702/1‑140 (MQ=255)                                                                   aGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGTACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATa                                                               >  2:853830/1‑140 (MQ=255)                                                                     cAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGTACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATAcc                                                             <  2:34567/140‑1 (MQ=255)                                                                       gAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGTACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATACCAt                                                           <  1:431121/140‑1 (MQ=255)                                                                                      tCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGTACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATACCATCCCGACGCGGGAACg                                            >  2:451488/1‑140 (MQ=255)                                                                                                tCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGTACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACGGATGAGGGAACAGCTCGAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGcc                                  >  1:284785/1‑140 (MQ=255)                                                                                                tCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGTACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGcc                                  >  1:494172/1‑140 (MQ=255)                                                                                                tCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGTACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGcc                                  >  2:301531/1‑140 (MQ=255)                                                                                                    ggCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGTACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGAt                              <  2:258294/140‑1 (MQ=255)                                                                                                     gCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGTACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATc                             <  1:250023/140‑1 (MQ=255)                                                                                                       tttttCACGAGCCGGCGCTTTATCGCGTTTAAGTACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCgg                           <  2:376972/140‑1 (MQ=255)                                                                                                          ttCACGAGCCGGCGCTTTATCGCGTTTAAGTACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGttt                        >  1:867387/1‑140 (MQ=255)                                                                                                                  ccGGCGCTTTATCGCGTTTAAGTACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCg                <  1:951432/140‑1 (MQ=255)                                                                                                                           tATCGCGTTTAAGTACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACg       >  2:941033/1‑140 (MQ=255)                                                                                                                              cgcgTTTAAGTACTTTCACCTGCGCCA‑GGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATAc   <  1:243685/140‑1 (MQ=255)                                                                                                                                cgTTTAAGTACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATAcc  >  2:710685/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                    CGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACC  >  NC_000913/684806‑685072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑