Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,102,028	T→C	N34D (AAC→GAC)	csgF ←	curli assembly component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,102,028	0	T	C	100.0%	205.9 / NA	54	N34D (AAC→GAC)	csgF	curli assembly component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/22);  total (32/22)

TGTGACTGGATGGCCTGAGTAAAGTTATCTAACGCTGAGGGTGTTTCAATACCAAAGTCATCGTTATAGCTCGGATCTTTATAAGAGTTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTTACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTT  >  NC_000913/1101895‑1102149                                                                                                                                      |                                                                                                                          tgtgACTGGATGGCCTGAGTAAAGTTATCTAACGCTGAGGGTGTTTCAATACCAAAGTCATCGTTATAGCTCGGATCTTTATAAGAGTTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTCaccacc                                                                                                                     <  1:290289/140‑1 (MQ=255) tgtgACTGGATGGCCTGAGTAAAGTTATCTAACGCTGAGGGTGTTTCAATACCAAAGTCATCGTTATAGCTCGGATCTTTATAAGAGTTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTCaccacc                                                                                                                     <  1:859138/140‑1 (MQ=255) tgcgACTGGATGGCCTGAGTAAAGTTATCTAACGCTGAGGGTGTTTCAATACCAAAGTCATCGTTATAGCTCGGATCTTTATAAGAGTTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTCaccacc                                                                                                                     <  2:266269/137‑1 (MQ=255)  gtgACTGGATGGCCTGAGTAAAGTTATCTAACGCTGAGGGTGTTTCAATACCAAAGTCATCGTTATAGCTCGGATCTTTATAAGAGTTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTCAccacca                                                                                                                    <  1:534083/140‑1 (MQ=255)  gtgACTGGATGGCCTGAGTAAAGTTATCTAACGCTGAGGGTGTTTCAATACCAAAGTCATCGTTATAGCTCGGATCTTTATAAGAGTTTTGGACCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTCAccacca                                                                                                                    <  1:354949/140‑1 (MQ=255)   tgACTGGATGGCCTGAGTAAAGTTATCTAACGCTGAGGGTGTTTCAATACCAAAGTCATCGTTATAGCTCGGATCTTTATAAGAGTTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTCACCACCaa                                                                                                                   >  2:644175/1‑140 (MQ=255)                   gTAAAGTTATCTAACGCTGAGGGTGTTTCAATACCAAAGTCATCGTTATAGCTCGGATCTTTATAAGAGTTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTCACCACCAAAGTTTGGATTACGGaa                                                                                                   >  2:161169/1‑140 (MQ=255)                    tAAAGTTATCTAACGCTGAGGGTGTTTCAATACCAAAGTCATCGTTATAGCTCGGATCTTTATAAGAGTTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTCACCACCAAAGTTTGGATTACGGAAc                                                                                                  >  1:401369/1‑140 (MQ=255)                       aGTTATCTAACGCTGAGGGTGTTTCAATACCAAAGTCATCGTTATAGCTCGGATCTTTATAAGAGTTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTCACCACCAAAGTTTGGATTACGGAACTgg                                                                                               >  2:335914/1‑140 (MQ=255)                            tCTAACGCTGAGGGTGTTTCAATACCAAAGTCATCGTTATAGCTCGGATCTTTATAAGAGTTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTCACCACCAAAGTTTGGATTACGGAACTGGAAAGt                                                                                          >  2:68404/1‑140 (MQ=255)                              tAACGCTGAGGGTGTTTCAATACCAAAGTCATCGTTATAGCTCGGATCTTTATAAGAGTTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTCACCACCAAAGTTTGGATTACGGAACTGGAAAGTCa                                                                                        >  1:700485/1‑140 (MQ=255)                              tAACGCTGAGGGTGTTTCAATACCAAAGTCATCGTTATAGCTCGGATCTTTATAAGAGTTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTCACCACCAAAGTTTGGATTACGGAACTGGAAAGTCa                                                                                        <  1:68404/140‑1 (MQ=255)                                      aGGGTGTTTCAATACCAAAGTCATCGTTATAGCTCGGATCTTTATAAGAGTTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTCACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCa                                                                                >  1:405326/1‑140 (MQ=255)                                      aGGGTGTTTCAATACCAAAGTCATCGTTATAGCTCGGATCTTTATAAGAGTTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTCACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCa                                                                                >  2:244029/1‑140 (MQ=255)                                      aGGGTGTTTCAATACCAAAGTCATCGTTATAGCTCGGATCTTTATAAGAGTTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTCACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCa                                                                                >  2:48852/1‑140 (MQ=255)                                      aGGGTGTTTCAATACCAAAGTCATCGTTATAGCTCGGATCTTTATAAGAGTTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTCACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCa                                                                                >  1:456235/1‑140 (MQ=255)                                         gtgtTTCAATACCAAAGTCATCGTTATAGCTCGGATCTTTATAAGAGTTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTCACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGcc                                                                             <  2:443194/140‑1 (MQ=255)                                         gtgtTTCAATACCAAAGTCATCGTTATAGCTCGGATCTTTATAAGAGTTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTCACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGcc                                                                             <  1:244029/140‑1 (MQ=255)                                                  tACCAAAGTCATCGTTATAGCTCGGATCTTTATAAGAGTTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTCACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAAt                                                                    >  1:870006/1‑140 (MQ=255)                                                    ccAAAGTCATCGTTATAGCTCGGATCTTTATAAGAGTTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTCACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATgg                                                                  >  1:697857/1‑140 (MQ=255)                                                       aaGTCATCGTTATAGCTCGGATCTTTATAAGAGTTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTCACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGa                                                               >  2:29148/1‑140 (MQ=255)                                                       aaGTCATCGTTATAGCTCGGATCTTTATAAGAGTTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTCACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGa                                                               >  1:657171/1‑140 (MQ=255)                                                         gTCATCGTTATAGCTCGGATCTTTATAAGAGTTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTCACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGaaa                                                             <  1:48852/140‑1 (MQ=255)                                                         gTCATCGTTATAGCTCGGATCTTTATAAGAGTTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTCACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGaaa                                                             <  2:697857/140‑1 (MQ=255)                                                            aTCGTTATAGCTCGGATC‑TTATAAGAGTTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTCACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAg                                                         >  2:68323/1‑140 (MQ=255)                                                             tCGTTATAGCTCGGATCTTTATAAGAGTTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTCACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAg                                                         >  2:655845/1‑140 (MQ=255)                                                              cGTTATAGCTCGGATCTTTATAAGAGTTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTCACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGc                                                        <  2:401369/140‑1 (MQ=255)                                                              cGTTATAGCTCGGATCTTTATAAGAGTTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTCACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGc                                                        >  1:442722/1‑140 (MQ=255)                                                              cGTTATAGCTCGGATCTTTATAAGAGTTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTCACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGc                                                        >  1:331981/1‑140 (MQ=255)                                                                        cGGATCTTTATAAGAGTTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTCACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGaa                                              >  2:952018/1‑140 (MQ=255)                                                                        cGGATCTTTATAAGAGTTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTCACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGaa                                              <  2:946999/140‑1 (MQ=255)                                                                         ggATCTTTATAAGAGTTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTCACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAac                                             >  1:627881/1‑140 (MQ=255)                                                                          gATCTTTATAAGAGTTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTCACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATg                                                     >  2:195032/1‑131 (MQ=255)                                                                          gATCTTTATAAGAGTTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTCACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATg                                                     <  1:195032/131‑1 (MQ=255)                                                                          gATCTTTATAAGAGTTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTCACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAact                                            >  2:930685/1‑140 (MQ=255)                                                                           aTCTTTATAAGAGTTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTCACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAacta                                           >  2:62593/1‑140 (MQ=255)                                                                           aTCTTTATAAGAGTTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTCACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAacta                                           <  1:335914/140‑1 (MQ=255)                                                                           aTCTTTATAAGAGTTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTCACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAacta                                           >  2:968321/1‑140 (MQ=255)                                                                             cTTTATAAGAGTTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTCACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAactact                                         <  2:456235/140‑1 (MQ=255)                                                                              tttATAAGAGTTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTCACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAACTACTg                                        <  2:331981/140‑1 (MQ=255)                                                                                tataAGAGTTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTCACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAACTACTGCa                                      >  1:320638/1‑140 (MQ=255)                                                                                         tttGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTCACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAACTACTGCATGTTTGaca                             <  2:320638/140‑1 (MQ=255)                                                                                         tttGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTCACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAACTACTGCATGTTTGaca                             <  1:644175/140‑1 (MQ=255)                                                                                          ttGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTCACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAACTACTGCATGTTTGacac                            >  1:529252/1‑140 (MQ=255)                                                                                                  gAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTCACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAACTACTGCATGTTTGACACGCATTGCa                    >  1:187327/1‑140 (MQ=255)                                                                                                  gAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTCACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAACTACTGCATGTTTGACACGCATTGCa                    >  2:259362/1‑140 (MQ=255)                                                                                                  gAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTCACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAACTACTGCATGTTTGACACGCATTGCa                    >  1:695178/1‑140 (MQ=255)                                                                                                   aGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTCACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAACTACTGCATGTTTGACACGCATTGCAg                   <  2:529252/140‑1 (MQ=255)                                                                                                          tttAATAAAAAAGCGCCATTATTTGGGTCACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGa            <  1:930685/140‑1 (MQ=255)                                                                                                                  aaaaGCGCCATTATTTGGGTCACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAAtttt    <  2:442722/140‑1 (MQ=255)                                                                                                                  aaaaGCGCCATTATTTGGGTCACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAAtttt    >  1:171353/1‑140 (MQ=255)                                                                                                                  aaaaGCGCCATTATTTGGGTCACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGAATGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAAt       <  2:605310/137‑1 (MQ=255)                                                                                                                  aaaaGCGCCATTATTTGGGTCACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGAATGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAAt       >  1:605310/1‑137 (MQ=255)                                                                                                                    aaGCGCCATTATTTGGGTCACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAAtttttt  >  1:461/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                          TGTGACTGGATGGCCTGAGTAAAGTTATCTAACGCTGAGGGTGTTTCAATACCAAAGTCATCGTTATAGCTCGGATCTTTATAAGAGTTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTTACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTT  >  NC_000913/1101895‑1102149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑