Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,190,646	T→C	intergenic (‑159/+89)	sroH ← / ← thiH	small RNA SroH/2‑iminoacetate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,190,646	0	T	C	100.0%	212.7 / NA	56	intergenic (‑159/+89)	sroH/thiH	small RNA SroH/2‑iminoacetate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/29);  total (27/29)

TTACTCTCTTACAATCAGATCTCTTTCATTGCTCAACAGGCGATGGCTTCAGACTTTGCATTACGGAATTTTTAAGAAAGGCAGGGCGAAACGAGGAAGAAGCTTTTCGCCCTGGACAACATTCCTGCTGACGGCACTACCATAAAAAAACACAACGCTTTCAACAACCTTGCGTTTTTCATCTTCCGTAGCGGCTCGGAATAAATTTACGTTCTTACATTCCGTCTCATAGTCTTTGCGAGGCGCGTCCCAGATAGCTGTCCCAGTCTTTC  >  NC_000913/4190509‑4190780                                                                                                                                          |                                                                                                                                       ttACTCTCTTACAATCAGATCTCTTTCATTGCTCAACAGGCGATGGCTTCAGACTTTGCATTACGGAATTTTTAAGAAAGGCAGGGCGAAACGAGGAAGAAGCTTTTCGCCCTGGACAACATTCCTGCTGACGGcaccac                                                                                                                                      >  2:925590/1‑140 (MQ=255) ttACTCTCTTACAATCAGATCTCTTTCATTGCTCAACAGGCGATGGCTTCAGACTTTGCATTACGGAATTTTTAAGAAAGGCAGGGCGAAACGAGGAAGAAGCTTTTCGCCCTGGACAACATTCCTGCTGACGGcaccac                                                                                                                                      >  2:722398/1‑140 (MQ=255)   aCTCTCTTACAATCAGATCTCTTTCATTGCTCAACAGGCGATGGCTTCAGACTTTGCATTACGGAATTTTTAAGAAAGGCAGGGCGAAACGAGGAAGAAGCTTTTCGCCCTGGACAACATTCCTGCTGACGGCAccacca                                                                                                                                    >  2:195735/1‑140 (MQ=255)        ctTACAATCAGATCTCTTTCATTGCTCAACAGGCGATGGCTTCAGACTTTGCATTACGGAATTTTTAAGAAAGGCAGGGCGAAACGAGGAAGAAGCTTTTCGCCCTGGACAACATTCCTGCTGACGGCACCACCATaaaa                                                                                                                               >  1:439922/1‑140 (MQ=255)            cAATCAGATCTCTTTCATTGCTCAACAGGCGATGGCTTCAGACTTTGCATTACGGAATTTTTAAGAAAGGCAGGGCGAAACGAGGAAGAAGCTTTTCGCCCTGGACAACATTCATGCTGACGGCACCACCATAAAAAAac                                                                                                                           >  1:710773/1‑140 (MQ=255)               tCAGATCTCTTTCATTGCTCAACAGGCGATGGCTTCAGACTTTGCATTACGGAATTTTTAAGAAAGGCAGGGCGAAACGAGGAAGAAGCTTTTCGCCCTGGACAACATTCCTGCTGACGGCACCACCATAAAAAAAcaca                                                                                                                        >  1:215529/1‑140 (MQ=255)                  gATCTCTTTCATTGCTCAACAGGCGATGGCTTCAGACTTTGCATTACGGAATTTTTAAGAAAGGCAGGGCGAAACGAGGAAGAAGCTTTTCGCCCTGGACAACATTCCTGCTGACGGCACCACCATAAAAAAACACAACg                                                                                                                     <  1:722398/140‑1 (MQ=255)                  gATCTCTTTCATTGCTCAACAGGCGATGGCTTCAGACTTTGCATTACGGAATTTTTAAGAAAGGCAGGGCGAAACGAGGAAGAAGCTTTTCGCCCTGGACAACATTCCTGCTGACGGCACCACCATAAAAAAACACAACg                                                                                                                     <  1:826921/140‑1 (MQ=255)                   aTCTCTTTCATTGCTCAACAGGCGATGGCTTCAGACTTTGCATTACGGAATTTTTAAGAAAGGCAGGGCGAAACGAGGAAGAAGCTTTTCGCCCTGGACAACATTCCTGCTGACGGCACCACCATAAAAAAACACAACGc                                                                                                                    >  2:896447/1‑140 (MQ=255)                   aTCTCTTTCATTGCTCAACAGGCGATGGCTTCAGACTTTGCATTACGGAATTTTTAAGAAAGGCAGGGCGAAACGAGGAAGAAGCTTTTCGCCCTGGACAACATTCCTGCTGACGGCACCACCATAAAAAAACACAACGc                                                                                                                    >  2:774545/1‑140 (MQ=255)                    tctcTTTCATTGCTCAACAGGCGATGGCTTCAGACTTTGCATTACGGAATTTTTAAGAAAGGCAGGGCGAAACGAGGAAGAAGCTTTTCGCCCTGGACAACATTCCTGCTGACGGCACCACCATAAAAAAACACAACGCt                                                                                                                   <  2:129352/140‑1 (MQ=255)                       ctTTCATTGCTCAACAGGCGATGGCTTCAGACTTTGCATTACGGAATTTTTAAGAAAGGCAGGGCGAAACGAGGAAGAAGCTTTTCGCCCTGGACAACATTCCTGCTGACGGCACCACCATAAAAAAACACAACGCTTTc                                                                                                                >  2:463670/1‑140 (MQ=255)                                cTCAACAGGCGATGGCTTCAGACTTTGCATTACGGAATTTTTAAGAAAGGCAGGGCGAAACGAGGAAGAAGCTTTTCGCCCTGGACAACATTCCTGCTGACGGCACCACCATAAAAAAACACAACGCTTTCAACAACCtt                                                                                                       >  1:526360/1‑140 (MQ=255)                                cTCAACAGGCGATGGCTTCAGACTTTGCATTACGGAATTTTTAAGAAAGGCAGGGCGAAACGAGGAAGAAGCTTTTCGCCCTGGACAACATTCCTGCTGACGGCACCACCATAAAAAAACACAACGCTTTCAACAACCtt                                                                                                       >  1:352594/1‑140 (MQ=255)                                cTCAACAGGCGATGGCTTCAGACTTTGCATTACGGAATTTTTAAGAAAGGCAGGGCGAAACGAGGAAGAAGCTTTTCGCCCTGGACAACATTCCTGCTGACGGCACCACCATAAAAAAACACAACGCTTTCAACAACCtt                                                                                                       >  1:246596/1‑140 (MQ=255)                                  cAACAGGCGATGGCTTCAGACTTTGCATTACGGAATTTTTAAGAAAGGCAGGGCGAAACGAGGAAGAAGCTTTTCGCCCTGGACAACATTCCTGCTGACGGCACCACCATAAAAAAACACAACGCTTTCAACAACCTTGc                                                                                                     >  1:226570/1‑140 (MQ=255)                                     cAGGCGATGGCTTCAGACTTTGCATTACGGAATTTTTAAGAAAGGCAGGGCGAAATGAGGAAGAAGCTTTTCGCCCTGGACAACATTCCTGCTGACGGCACCACCATAAAAAAACACAACGCTTTCAACAACCTTGCGtt                                                                                                  >  2:943781/1‑140 (MQ=255)                                     cAGGCGATGGCTTCAGACTTTGCATTACGGAATTTTTAAGAAAGGCAGGGCGAAACGAGGAAGAAGCTTTTCGCCCTGGACAACATTCCTGCTGACGGCACCACCATAAAAAAACACAACGCTTTCAACAACCTTGCGtt                                                                                                  >  1:179577/1‑140 (MQ=255)                                          gATGGCTTCAGACTTTGCATTACGGAATTTTTAAGAAAGGCAGGGCGAAACGAGGAAGAAGCTTTTCGCCCTGGACAACATTCCTGCTGACGGCACCACCATAAAAAAACACAACGCTTTCAACAACCTTGCGtttttaa                                                                                             <  1:904628/140‑3 (MQ=255)                                          gATGGCTTCAGACTTTGCATTACGGAATTTTTAAGAAAGGCAGGGCGAAACGAGGAAGAAGCTTTTCGCCCTGGACAACATTCCTGCTGACGGCACCACCATAAAAAAACACAACGCTTTCAACAACCTTGCGTTTTTCa                                                                                             >  1:706076/1‑140 (MQ=255)                                          gATGGCTTCAGACTTTGCATTACGGAATTTTTAAGAAAGGCAGGGCGAAACGAGGAAGAAGCTTTTCGCCCTGGACAACATTCCTGCTGACGGCACCACCATAAAAAAACACAACGCTTTCAACAACCTTGCGTTTTTCa                                                                                             >  2:882265/1‑140 (MQ=255)                                             ggCTTCAGACTTTGCATTACGGAATTTTTAAGAAAGGCAGGGCGAAACGAGGAAGAAGCTTTTCGCCCTGGACAACATTCCTGCTGACGGCACCACCATAAAAAAACACAACGCTTTCAACAACCTTg                                                                                                      <  1:763228/128‑1 (MQ=255)                                             ggCTTCAGACTTTGCATTACGGAATTTTTAAGAAAGGCAGGGCGAAACGAGGAAGAAGCTTTTCGCCCTGGACAACATTCCTGCTGACGGCACCACCATAAAAAAACACAACGCTTTCAACAACCTTg                                                                                                      >  2:763228/1‑128 (MQ=255)                                             ggCTTCAGACTTTGCATTACGGAATTTTTAAGAAAGGCAGGGCGAAACGAGGAAGAAGCTTTTCGCCCTGGACAACATTCCTGCTGACGGCACCACCATAAAAAAACACAACGCTTTCAACAACCTTGCGTTTTTCATCt                                                                                          >  2:105134/1‑140 (MQ=255)                                                  cAGACTTTGCATTACGGAATTTTTAAGAAAGGCAGGGCGAAACGAGGAAGAAGCTTTTCGCCCTGGACAACATTCCTGCTGACGGCACCACCATAAAAAAACACAACGCTTTCAACAACCTTGCGt                                                                                                   <  2:9284/126‑1 (MQ=255)                                                  cAGACTTTGCATTACGGAATTTTTAAGAAAGGCAGGGCGAAACGAGGAAGAAGCTTTTCGCCCTGGACAACATTCCTGCTGACGGCACCACCATAAAAAAACACAACGCTTTCAACAACCTTGCGt                                                                                                   >  1:9284/1‑126 (MQ=255)                                                  cAGACTTTGCATTACGGAATTTTTAAGAAAGGCAGGGCGAAACGAGGAAGAAGCTTTTCGCCCTGGACAACATTCCTGCTGACGGCACCACCATAAAAAAACACAACGCTTTCAACAACCTTGCGTTTTTCATCTTCTGt                                                                                     >  1:187372/1‑140 (MQ=255)                                                  cAGACTTTGCATTACGGAATTTTTAAGAAAGGCAGGGCGAAACGAGGAAGAAGCTTTTCGCCCTGGACAACATTCCTGCTGACGGCACCACCATAAAAAAACACAACGCTTTCAACAACCTTGCGTTTTTCATCTTCCGt                                                                                     >  2:476067/1‑140 (MQ=255)                                                        ttGCATTACGGAATTTTTAAGAAAGGCAGGGCGAAATGAGGAAGAAGCTTTTCGCCCTGGACAACATTCCTGCTGACGGCACCACCATAAAAAAACACAACGCTTTCAACAACCTTGCGTTTTTCATCTTCCGTAGCGGc                                                                               <  1:943781/140‑1 (MQ=255)                                                         tGCATTACGGAATTTTTAAGAAAGGCAGGGCGAAACGAGGAAGAAGCTTTTCGCCCTGGACAACATTCCTGCTGACGGCACCACCATAAAAAAACACAACGCTTTCAACAACCTTGCGTTTTTCATCTTCCGTAGCGGCt                                                                              <  1:463670/140‑1 (MQ=255)                                                         tGCATTACGGAATTTTTAAGAAAGGCAGGGCGAAACGAGGAAGAAGCTTTTCGCCCTGGACAACATTCCTGCTGACGGCACCACCATAAAAAAACACAACGCTTTCAACAACCTTGCGTTTTTCATCTTCCGTAGCGGCt                                                                              <  1:871756/140‑1 (MQ=255)                                                         tGCATTACGGAATTTTTAAGAAAGGCAGGGCGAAACGAGGAAGAAGCTTTTCGCCCTGGACAACATTCCTGCTGACGGCACCACCATAAAAAAACACAACGCTTTCAACAACCTTGCGTTTTTCATCTTCCGTAGCGGCt                                                                              <  1:476067/140‑1 (MQ=255)                                                         tGCATTACGGAATTTTTAAGAAAGGCAGGGCGAAACGAGGAAGAAGCTTTTCGCCCTGGACAACATTCCTGCTGACGGCACCACCATAAAAAAACACAACGCTTTCAACAACCTTGCGTTTTTCATCTTCCGTAGCGGCt                                                                              <  2:706076/140‑1 (MQ=255)                                                         tGCATTACGGAATTTTTAAGAAAGGCAGGGCGAAACGAGGAAGAAGCTTTTCGCCCTGGACAACATTCCTGCTGACGGCACCACCATAAAAAAACACAACGCTTTCAACAACCTTGCGTTTTTCATCTTCCGTAGCGGCt                                                                              >  2:455912/1‑140 (MQ=255)                                                             ttACGGAATTTTTAAGAAAGGCAGGGCGAAACGAGGAAGAAGCTTTTCGCCCTGGACAACATTCCTGCTGACGGCACCACCATAAAAAAACACAACGCTTTCAACAACCTTGCGTTTTTCATCTTCCGTAGCGGCTCGGa                                                                          <  2:439922/140‑1 (MQ=255)                                                               aCGGAATTTTTAAGAAAGGCAGGGCGAAACGAGGAAGAAGCTTTTCGCCCTGGACAACATTCCTGCTGACGGCACCACCATAAAAAAACACAACGCTTTCAACAACCTTGCGTTTTTCATCTTCCGTAGCGGCTCGGAAt                                                                        <  1:443009/140‑1 (MQ=255)                                                                    aTTTTTAAGAAAGGCAGGGCGAAACGAGGAAGAAGCTTTTCGCCCTGGACAACATTCCTGCTGACGGCACCACCATAAAAAAACACAACGCTTTCAACAACCTTGCGTTTTTCATCTTCCGTAGCGGCTCGGAATAAAtt                                                                   <  2:215529/140‑1 (MQ=255)                                                                      ttttAAGAAAGGCAGGGCGAAACGAGGAAGAAGCTTTTCGCCCTGGACAACATTCCTGCTGACGGCACCACCATAAAAAAACACAACGCTTTCAACAACCTTGCGTTTTTCATCTTCCGTAGCGGCTCGGAATAAATTTa                                                                 <  2:119713/140‑1 (MQ=255)                                                                      ttttAAGAAAGGCAGGGCGAAACGAGGAAGAAGCTTTTCGCCCTGGACAACATTCCTGCTGACGGCACCACCATAAAAAAACACAACGCTTTCAACAACCTTGCGTTTTTCATCTTCCGTAGCGGCTCGGAATAAATTTa                                                                 <  2:579276/140‑1 (MQ=255)                                                                      ttttAAGAAAGGCAGGGCGAAACGAGGAAGAAGCTTTTCGCCCTGGACAACATTCCTGCTGACGGCACCACCATAAAAAAACACAACGCTTTCAACAACCTTGCGTTTTTCATCTTCCGTAGCGGCTCGGAATAAATTTa                                                                 <  1:446828/140‑1 (MQ=255)                                                                        ttAAGAAAGGCAGGGCGAAACGAGGAAGAAGCTTTTCGCCCTGGACAACATTCCTGCTGACGGCACCACCATAAAAAAACACAACGCTTTCAACAACCTTGCGTTTTTCATCTTCCGTAGCGGCTCGGAATAAATTTACg                                                               <  1:896447/140‑1 (MQ=255)                                                                               aGGCAGGGCGAAACGAGGAAGAAGCTTTTCGCCCTGGACAACATTCCTGCTGACGGCACCACCATAAAAAAACACAACGCTTTCAACAACCTTGCGTTTTTCATCTTCCGTAGCGGCTCGGAATAAATTTACGTTCTTAc                                                        >  1:611239/1‑140 (MQ=255)                                                                                ggCAGGGCGAAACGAGGAAGAAGCTTTTCGCCCTGGACAACATTCCTGCTGACGGCACCACCATAAAAAAACACAACGCTTTCAACAACCTTGCGTTTTTCATCTTCCGTAGCGGCTCGGAATAAATTTACGTTCTTACa                                                       <  1:774545/140‑1 (MQ=255)                                                                                 gCAGGGCGAAACGAGGAAGAAGCTTTTCGCCCTGGACAACATTCCTGCTGACGGCACCACCATAAAAAAACACAACGCTTTCAACAACCTTGCGTTTTTCATCTTCCGTAGCGGCTCGGAATAAATTTACg                                                               >  2:4971/1‑131 (MQ=255)                                                                                 gCAGGGCGAAACGAGGAAGAAGCTTTTCGCCCTGGACAACATTCCTGCTGACGGCACCACCATAAAAAAACACAACGCTTTCAACAACCTTGCGTTTTTCATCTTCCGTAGCGGCTCGGAATAAATTTACg                                                               <  1:4971/131‑1 (MQ=255)                                                                                 gCAGGGCGAAACGAGGAAGAAGCTTTTCGCCCTGGACAACATTCCTGCTGACGGCACCACCATAAAAAAACACAACGCTTTCAACAACCTTGCGTTTTTCATCTTCCGTAGCGGCTCGGAATAAATTTACGTTCTTACAt                                                      >  2:849024/1‑140 (MQ=255)                                                                                    gggCGAAACGAGGAAGAAGCTTTTCGCCCTGGACAACATTCCTGCTGACGGCACCACCATAAAAAAACACAACGCTTTCAACAACCTTGCGTTTTTCATCTTCCGTAGCGGCTCGGAATAAATTTACGTTCTTACATTcc                                                   <  2:179577/140‑1 (MQ=255)                                                                                    gggCGAAACGAGGAAGAAGCTTTTCGCCCTGGACAACATTCCTGCTGACGGCACCACCATAAAAAAACACAACGCTTTCAACAACCTTGCGTTTTTCATCTTCCGTAGCGGCTCGGAATAAATTTACGTTCTTACATTcc                                                   <  1:195735/140‑1 (MQ=255)                                                                                                          ttCGCCCTGGACAACATTCCTGCTGACGGCACCACCATAAAAAAACACAACGCTTTCAACAACCTTGCGTTTTTCATCTTCCGTAGCGGCTCGGAATAAATTTACGTTCTTACATTCCGTCTCATAGTCTTTGCGAGgcg                             >  2:770365/1‑140 (MQ=255)                                                                                                          ttCGCCCTGGACAACATTCCTGCTGACGGCACCACCATAAAAAAACACAACGCTTTCAACAACCTTGCGTTTTTCATCTTCCGTAGCGGCTCGGAATAAATTTACGTTCTTACATTCCGTCTCATAGTCTTTGCGAGgcg                             <  2:860337/140‑1 (MQ=255)                                                                                                          ttCGCCCTGGACAACATTCCTGCTGACGGCACCACCATAAAAAAACACAACGCTTTCAACAACCTTGCGTTTTTCATCTTCCGTAGCGGCTCGGAATAAATTTACGTTCTTACATTCCGTCTCATAGTCTTTGCGAGgcg                             <  1:455912/140‑1 (MQ=255)                                                                                                            cGCCCTGGACAACATTCCTGCTGACGGCACCACCATAAAAAAACACAACGCTTTCAACAACCTTGCGTTTTTCATCTTCCGTAGCGGCTCGGAATAAATTTACGTTCTTACATTCCGTCTCATAGTCTTTGCGAGGcgcg                           <  1:770365/140‑1 (MQ=255)                                                                                                            cGCCCTGGACAACATTCCTGCTGACGGCACCACCATAAAAAAACACAACGCTTTCAACAACCTTGCGTTTTTCATCTTCCGTAGCGGCTCGGAATAAATTTACGTTCTTACATTCCGTCTCATAGTCTTTGCGAGGcgcg                           <  1:228588/140‑1 (MQ=255)                                                                                                            cGCCCTGGACAACATTCCTGCTGACGGCACCACCATAAAAAAACACAACGCTTTCAACAACCTTGCGTTTTTCATCTTCCGTAGCGGCTCGGAATAAATTTACGTTCTTACATTCCGTCTCATAGTCTTTGCGAGGcgcg                           <  2:122315/140‑1 (MQ=255)                                                                                                                      aacaTTCCTGCTGACGGCACCACCATAAAAAAACACAACGCTTTCAACAACCTTGCGTTTTTCATCTTCCGTAGCGGCTCGGAATAAATTTACGTTCTTACATTCCGTCTCATAGTCTTTGCGAGGCGCGTCCCAGATAg                 <  2:526360/140‑1 (MQ=255)                                                                                                                        caTTCCTGCTGACGGCACCACCATAAAAAAACACAACGCTTTCAACAACCTTGCGTTTTTCATCTTCCGTAGCGGCTCGGAATAAATTTACGTTCTTACATTCCGTCTCATAGTCTTTGCGAGGCGCGTCCCAGATAGCt               >  1:142046/1‑140 (MQ=255)                                                                                                                               gctgACGGCACCACCATAAAAAAACACAACGCTTTCAACAACCTTGCGTTTTTCATCTTCCGTAGCGGCTCGGAATAAATTTACGTTCTTACATTCCGTCTCATAGTCTTTGCGAGGCGCGTCCCAGATAGCTGTCCCAg        <  2:699457/140‑1 (MQ=255)                                                                                                                                  gACGGCACCACCATAAAAAAACACAACGCTTTCAACAACCTTGCGTTTTTCATCTTCCGTAGCGGCTCGGAATAAATTTACGTTCTTACATTCCGTCTCATAGTCTTTGCGAGGCGCGTCCCAGATAGCTGTCCCAGTCt     >  1:474236/1‑140 (MQ=255)                                                                                                                                   aCGGCACCACCATAAAAAAACACAACGCTTTCAACAACCTTGCGTTTTTCATCTTCCGTAGCGGCTCGGAATAAATTTACGTTCTTACATTCCGTCTCATAGTCTTTGCGAGGCGCGTCCCAGATAGCTGTCCCAGTCtt    >  1:651215/1‑140 (MQ=255)                                                                                                                                     ggCACCACCATAAAAAAACACAACGCTTTCAACAACCTTGCGTTTTTCATCTTCCGTAGCGGCTCGGAATAAATTTACGTTCTTACATTCCGGCTCATATTCTTTTCGAAGCCCGTCCCAGATAGCTGTCCCAGTCTTTc  >  1:451104/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       TTACTCTCTTACAATCAGATCTCTTTCATTGCTCAACAGGCGATGGCTTCAGACTTTGCATTACGGAATTTTTAAGAAAGGCAGGGCGAAACGAGGAAGAAGCTTTTCGCCCTGGACAACATTCCTGCTGACGGCACTACCATAAAAAAACACAACGCTTTCAACAACCTTGCGTTTTTCATCTTCCGTAGCGGCTCGGAATAAATTTACGTTCTTACATTCCGTCTCATAGTCTTTGCGAGGCGCGTCCCAGATAGCTGTCCCAGTCTTTC  >  NC_000913/4190509‑4190780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑