| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 650,809 | 0 | T | G | 35.4% | 68.1 / 17.8 | 48 | T350P (ACC→CCC) | citC | citrate lyase synthetase |
| Reads supporting (aligned to +/- strand): ref base T (12/19); new base G (17/0); total (29/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.26e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑C‑AAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCGGATA > NC_000913/650677‑650946 | ctgctATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTTATTTTTCACCTGTTTTTCCTGCGGGGGGCttttta > 2:611537/1‑138 (MQ=255) ctATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTATTTTTTTTTTTCAACTGTTTCTCGTGCGGGGGTCttttttcgg > 1:894103/1‑135 (MQ=255) tATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAg < 1:378441/140‑1 (MQ=255) gATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTTTTTTTATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGc > 2:674947/1‑140 (MQ=255) cAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCCCGTc > 1:775682/1‑140 (MQ=255) gATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCg > 2:604042/1‑140 (MQ=255) tCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAg < 1:422225/140‑1 (MQ=255) ccTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGt < 2:591494/140‑1 (MQ=255) tGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGc > 1:1120333/1‑140 (MQ=255) tGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAACTGCCCCGTCCCGGCgggggggg > 2:356764/1‑135 (MQ=255) gCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTTTTTTCATTTTTTACCTGTTTTTCATGCGGGGGGCTTTTGACGAGCTGCCGCGGCCTGGCggggggggc > 2:712263/1‑134 (MQ=255) gCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTATTTTCATTTTTCACCCGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGTGcc > 2:536102/1‑139 (MQ=255) cGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑c > 1:247787/1‑140 (MQ=255) cGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑C‑AAGCa > 1:1212088/1‑140 (MQ=255) ggggCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑C‑AAGCAGGTTc < 2:607232/140‑1 (MQ=255) gggCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑C‑AAGCAGGTTCt < 2:1120333/140‑1 (MQ=255) ggCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑C‑AAGCAGGTTCt < 2:198988/139‑1 (MQ=255) ggCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑C‑AAGCAGGTTCt > 1:198988/1‑139 (MQ=255) ggCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGGT‑C‑AAACAGGGTCtt > 2:961178/1‑139 (MQ=255) gCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑C‑AAGCAGGTTCTGc < 1:794798/140‑1 (MQ=255) gCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑C‑AAGCAGGTTCTGc < 1:1120093/140‑1 (MQ=255) gCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGGT‑C‑CAGCAGGGTCttccaaaa > 2:472699/1‑133 (MQ=255) tATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTTATTTTTTACCTGTTTTTCATGCGGGGGTCTTTTGACGAACTGCCGCGTCCTGGCGGGGGGGCT‑C‑AAACAGGGTCTTCaaaaaaag > 2:510996/1‑138 (MQ=255) tATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑C‑AAGCAGGTTCTGCAAATAATg < 1:40409/140‑1 (MQ=255) atcatcACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTTACCTGTTTTTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGGT‑C‑AAACAGGGTCTTCAAAAAAAGc > 2:1185393/1‑140 (MQ=255) tcaCATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTATTTTTATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTTACGAGCTGCCGCGTCCTGGCGGGGGGGGT‑CTAAGCAGGTTTTCCAaaaaatccgtc > 2:1115008/1‑135 (MQ=255) ccccAGACTCAAGGGTGCCTGCAACGTCGGGCTGGTTTTTTTTTTTTTTTCACCTGTTTTTTATGTGGGGGGGTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTCC‑AGGCAGG‑TATTCAAATAATGCAGccggagtg > 2:798642/1‑133 (MQ=255) ccccAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCA‑TTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGGGGT‑C‑AAACAGGGTCTGCAAATAAAGCAGCGTGACTGc > 1:809453/1‑140 (MQ=255) cAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCT‑C‑AAGCAGGTTCTTCCAAAAAAGCAGCGGGGCTGCAg > 2:1020586/1‑140 (MQ=255) aaGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGGGCT‑C‑AAGCAGGGTCTGCAAATAATGCAGCGTGACTGCAGGGGcccg > 1:774190/1‑138 (MQ=255) gCCTGCAACGGCGGGCTGGTTTTTTTTTCTTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGGT‑C‑AAACAGGTTTTGGAAATAATGGAGCGTGGCTGCAGGGACCAGCGgcgc > 2:915693/1‑140 (MQ=255) ccTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCT‑C‑AAGCAGGGTCTGCAAATAATGCAGCGTGACTGCAGGGGCCAGCGGcgcg > 1:920749/1‑140 (MQ=255) ccTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑C‑AAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGcgcg < 1:1298379/140‑1 (MQ=255) ccTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCT‑C‑AAGCAGGGTCTGCAAATAATGCAGCGGGACTGCAGGGGCCCGCGGggcg > 2:869090/1‑140 (MQ=255) ccTGCAACGGCGGGCTGGGTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCT‑C‑AAGCAGGGTCTGCAAAAAAAGCAGCGTGACTGCAGGGGCCAGCGGggcg > 2:852828/1‑140 (MQ=255) aCGGCGGGCTGGTTTATTTTCATTTTTTACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGGT‑C‑AAACAGGTTTTTCAAATAATGGCGCGGGGCTGCAGGGGGCCGCGGCGCGGTAGcc > 2:1017707/1‑140 (MQ=255) cggGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑C‑AAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTga < 1:604042/140‑1 (MQ=255) tttCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑C‑AAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGc < 1:472699/140‑1 (MQ=255) ttCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑C‑AAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGcc < 2:228767/140‑1 (MQ=255) ttCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAACTGCCGCGTCCTGGCGGGAGTGCT‑C‑AAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGcc < 1:510996/140‑1 (MQ=255) cATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑C‑AAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCcag < 1:915693/140‑1 (MQ=255) aTTTTTTACCTATTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGAT‑C‑AAGAAGGCTCTTCCAAAAATGCACCGTAACTGCAGGGACCAGCGGACCGATAGCCTTGAGATCGTTTTTTGCcagc > 2:371589/1‑140 (MQ=255) aCCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑C‑AAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGAc < 1:674947/140‑1 (MQ=255) ccTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑C‑AAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACg < 1:1185393/140‑1 (MQ=255) tttCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑C‑AAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTAcc > 2:894641/1‑140 (MQ=255) ttCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑C‑AAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTAccc < 1:961178/140‑1 (MQ=255) tGCGGGGGTCTTTTGATGAGCTGCCGCGTCCTGGCGGGAGTGCT‑C‑AAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAg > 2:528758/1‑140 (MQ=255) tGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑C‑AAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAg > 1:1389721/1‑140 (MQ=255) cGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑C‑AAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCg < 1:869090/140‑1 (MQ=255) cGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑C‑AAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCg < 2:1389721/140‑1 (MQ=255) gggggTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑C‑AAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCgg > 2:705304/1‑140 (MQ=255) gggggTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑C‑AAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCgg > 2:1368484/1‑140 (MQ=255) ggggTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑C‑AAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCGGa > 2:461100/1‑140 (MQ=255) ggTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑C‑AAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCGGata > 1:781548/1‑140 (MQ=255) | CTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑C‑AAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCGGATA > NC_000913/650677‑650946 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |