Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913501,5640AC25.0% 67.3 / 11.0 36*559E (TAA→GAA) ybaLputative transporter YbaL
Reads supporting (aligned to +/- strand):  ref base A (24/3);  new base C (0/9);  total (24/12)
Fisher's exact test for biased strand distribution p-value = 2.34e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGG  >  NC_000913/501426‑501700
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ttAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTAc                                                                                                                                         >  2:845758/1‑140 (MQ=255)
      tATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTc                                                                                                                                   <  1:606142/140‑1 (MQ=255)
       aTCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTAc                                                                                                                                         <  2:874998/133‑1 (MQ=255)
       aTCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTAc                                                                                                                                         >  1:874998/1‑133 (MQ=255)
       aTCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTca                                                                                                                                  >  2:606532/1‑140 (MQ=255)
        tcgtcgGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTcac                                                                                                                                 <  1:1136211/140‑1 (MQ=255)
          gtcgGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTcacca                                                                                                                               >  2:721979/1‑140 (MQ=255)
          gtcgGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTcacca                                                                                                                               >  1:253474/1‑140 (MQ=255)
             gggtCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGGTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCt                                                                                                                            >  2:1171985/4‑140 (MQ=255)
               ttCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCa                                                                                                                          >  1:868503/1‑140 (MQ=255)
                      gCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACccgccg                                                                                                                   >  2:162788/1‑140 (MQ=255)
                           aTAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGc                                                                                                              >  2:357705/1‑140 (MQ=255)
                           aTAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGc                                                                                                              >  2:1096306/1‑140 (MQ=255)
                           aTAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGc                                                                                                              >  1:242679/1‑140 (MQ=255)
                                gCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTc                                                                                                         >  1:366489/1‑140 (MQ=255)
                                   ttCCCGCCGCATCTGGCTTTTGGCTCTCGATGCCTGATGCGACGCGGGCGCTTTTTATCATGCCTACATTTTTTTCATATTTTACATCCGGCAACCCCCTTTTCCCCCGCCCCCCCCTCCCCCGCCGGTGGCGTTTCcag                                                                                                      <  2:868503/140‑1 (MQ=255)
                                     cACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCcagca                                                                                                    >  1:286012/1‑140 (MQ=255)
                                      tcccctcATCTGGAATTGGTCTCTTTATGCTGGATGCGGCCGTGGTGGGTATTCTCATCCATTTATATTTTCAATATTTTAGTGCCGCCACCCGCCGTTTCCCCCCTCGCCACCTCACCCGCCGGTGGCGTTTCcagcag                                                                                                   <  2:190279/134‑1 (MQ=255)
                                        gccgcATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGtt                                                                                                 >  1:190279/1‑140 (MQ=255)
                                          cgcATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTcc                                                                                               >  1:71805/1‑140 (MQ=255)
                                                  gCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCAtctg                                                                                       >  1:384529/1‑137 (MQ=255)
                                                    ttttGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCACCCACCGTTTCCCCCGTCCCCCCCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTAc                                                                                     <  1:443753/139‑1 (MQ=255)
                                                       tGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACggg                                                                                  >  1:760350/1‑140 (MQ=255)
                                                       tGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACggg                                                                                  >  1:603128/1‑140 (MQ=255)
                                                                  gCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATTTTTTTCATATTTTACACCCGGCACCCCCCGTTTCCCCCGTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGc                                                                       <  2:727963/140‑1 (MQ=38)
                                                                   ccTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTCCATCCGGCACCCCCCGTTTCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCt                                                                      <  2:321054/140‑1 (MQ=38)
                                                                       aTGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGcc                                                                  >  1:213277/1‑140 (MQ=255)
                                                                            ccGCTGGCGCTTTTTATCATCCCTACATTTTTTTCATTTTTTACACCCGGCACCCCCCGTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCa                                                             <  2:603128/139‑1 (MQ=255)
                                                                                gggCGCGTCTTATCATCCCTACATATTTTTCATTTTTTCCACCCGCCACCCCCCTTTTCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTAc                                                         <  2:366489/139‑1 (MQ=255)
                                                                                            ccATGCCTACATTTTTTTCATATTTTACACCCGGCACCCCCCGTTTCCCCCGTCACCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGcacc                                             <  1:162788/139‑1 (MQ=255)
                                                                                               tcccTCCATTTTTTTCATTTTTTCCATCCGACACCCCCCTTTTCCCCCGTCCCCCCCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACg                                          <  2:760350/138‑1 (MQ=255)
                                                                                                 ccTACTTTTTTTTCATTTTTTACCCCCGGCACCCCCCTTTTCCCCCGCCACCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGtt                                        <  2:253474/140‑1 (MQ=255)
                                                                                                 ccTACTTTTTTTTCATTTTTTACATCCGCCACCCCCCGTTTCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGtt                                        <  2:927569/140‑1 (MQ=255)
                                                                                                 ccTACATTTTTTTCATATTTTACACCCGGCACCCCCCTTTTCCCCCGTCCCCCCCCCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGtt                                        <  2:242679/140‑1 (MQ=255)
                                                                                                 ccTACATATTTTTAATTTTTTCCATCCGGCACCCCCGGTTTCCCCCGCCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGtt                                        <  2:786656/140‑1 (MQ=255)
                                                                                                  cTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGATGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTc                                       >  2:190961/1‑140 (MQ=255)
                                                                                                   tACATTTTTTTCATATTTTACATCCGGCACCCCCCTTTTCCCCCGCCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCg                                      <  2:423115/140‑1 (MQ=255)
                                                                                                    acttttttttCATATTTTACACCGGGCACCCCCGGTTTCCCCCGTCACCACCCCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCgg                                     <  1:721979/135‑1 (MQ=255)
                                                                                                    aattttttttCATTTTTTCCACCCGCCACCCCCCGTTCCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCgg                                     <  2:286012/135‑1 (MQ=255)
                                                                                                    aCATTTTTTTCATTTTTTACACCCGGCACCCCCCTTTTCCCCCGTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCgg                                     <  2:213277/140‑1 (MQ=255)
                                                                                                    aCATATTTTTCATTTTTTACACCCGGCAACCCCCGTTTCCCCCGTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCgg                                     <  1:337407/140‑1 (MQ=255)
                                                                                                    aCATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCgg                                     >  2:728685/1‑140 (MQ=255)
                                                                                                    aCATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCgg                                     >  1:786656/1‑140 (MQ=255)
                                                                                                     cATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGTGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGt                                    >  1:868743/1‑140 (MQ=255)
                                                                                                              tatttttaACACCCAGCACCCCCTGTTGCCCCCGCCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGc                           <  2:868743/136‑1 (MQ=255)
                                                                                                                    ttACACCCGCCACCCCCCGTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTc                     <  1:606532/140‑1 (MQ=255)
                                                                                                                         tCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGt                >  2:1119216/1‑140 (MQ=255)
                                                                                                                         cccGGCACCCCCCGTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGt                <  1:1096306/139‑1 (MQ=255)
                                                                                                                                  ccccGTTTCCCCCCCCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATggg       <  2:183878/138‑1 (MQ=255)
                                                                                                                                   cccGTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCTACTTCATCGTCATAATGGgc      <  1:212395/139‑1 (MQ=255)
                                                                                                                                     cttttCCCCCCTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGgcgc    <  2:1104041/138‑1 (MQ=255)
                                                                                                                                       tttACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCgg  >  2:814439/1‑140 (MQ=255)
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TTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGG  >  NC_000913/501426‑501700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑