Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4350CT34.9% 57.1 / 26.8 43intergenic (‑179/‑28)ttdR/ttdADNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (1/27);  new base T (15/0);  total (16/27)
Fisher's exact test for biased strand distribution p-value = 1.06e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGA  >  NC_000913/3206300‑3206566
                                                                                                                                        |                                                                                                                                   
aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGTAATTTTTCCaa                                                                                                                                  >  2:427270/1‑139 (MQ=255)
aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑GATTTTTTCAGa                                                                                                                                 >  2:30333/1‑140 (MQ=255)
aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGa                                                                                                                                 >  1:338901/1‑140 (MQ=255)
    ttGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACaaa                                                                                                                             <  1:773511/140‑1 (MQ=255)
               tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTg                                                                                                                  <  2:656387/140‑1 (MQ=255)
               tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCAAACAAAAAAAAAAAATg                                                                                                                  >  2:24152/1‑140 (MQ=255)
               tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCAAACAAAAAAAAAAAATg                                                                                                                  >  1:269206/1‑140 (MQ=255)
                  tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                               <  1:136557/140‑1 (MQ=255)
                  tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                               <  2:131990/140‑1 (MQ=255)
                      gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCACACAAAAAAAAAAAATAGAGGTgt                                                                                                           >  1:443118/1‑139 (MQ=255)
                           atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑GATTTTTTCAGACAAAAACAAAAAAAGGAGGTGCCatga                                                                                                      >  1:53848/1‑140 (MQ=255)
                                cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGAAAAAAACAAAAAATGGAGGTGGCATGATGAgg                                                                                                 >  1:331886/1‑139 (MQ=255)
                                  ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                               <  1:666100/140‑1 (MQ=255)
                                   tcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAAAAAAAAAAGGAGTTGTCCTGATGAGCGaa                                                                                              >  1:574137/1‑140 (MQ=255)
                                   tcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAAAAAAAAAAGGAGGTGCCATGATGAGGGaa                                                                                              >  2:153837/1‑140 (MQ=255)
                                    cTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGaaa                                                                                             <  2:1109394/140‑1 (MQ=255)
                                    cTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGaaa                                                                                             <  2:275382/140‑1 (MQ=255)
                                      tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAACAAAAAAAGGAGTTGGCATGATGAGCGaaaaa                                                                                           >  2:267612/1‑138 (MQ=255)
                                      tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGG‑GATTTTTTCAAACAAAAACAAAAAATGGAGGGTTCATGATTAGCGaaaaa                                                                                           >  2:863647/1‑138 (MQ=255)
                                        ttttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGG‑AATTTTTTTAGACAAAAAAAAAAAAAGGAGGTGGCATGATGAGGgaaagaaa                                                                                         >  2:686018/1‑140 (MQ=255)
                                               gTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCaa                                                                                  <  1:47404/140‑1 (MQ=255)
                                               gTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCaa                                                                                  <  1:16252/140‑1 (MQ=255)
                                                 tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAAAAAAAAATGGAGGTGGCCTGATGAGGGAAAGtaaaaaaaaaaa                                                                                >  2:604906/1‑134 (MQ=255)
                                                   aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                              <  2:467404/140‑1 (MQ=255)
                                                   aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                              <  1:267612/140‑1 (MQ=255)
                                                    ttCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGc                                                                             <  1:750751/140‑1 (MQ=255)
                                                          aaCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGaa                                                                       <  2:409006/140‑1 (MQ=255)
                                                               ttAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGt                                                                  <  1:863647/140‑1 (MQ=255)
                                                                tAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGtt                                                                 <  1:427270/140‑1 (MQ=255)
                                                                       atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAAAAAAAAAATGAGTTGCCCTTATGAGGGGAAATAAAAAAAAAAAAGCAGTGAAAAAGATGACagaa                                                          >  2:231793/1‑139 (MQ=255)
                                                                        tatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAgaga                                                         <  1:24152/140‑1 (MQ=255)
                                                                         atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAACAAAAAAAAAAAATTGAGGTGGCATTATGAGCGGAAAAAAAAAAAAAAAAGCAGGGAAAAAAATGACAgagaa                                                        >  2:302807/1‑139 (MQ=255)
                                                                         atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCAGAAAAAAAAAAAAAAAGGAGGTGGCATGATGAGGGGAAGTAAAAAACAAAAAGCAGTGAAAAAAATGACAgagaa                                                        >  2:652681/1‑139 (MQ=255)
                                                                            cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAACAAAAACAAAAAAAGGAGTTGCCCTGATGAGGGAAAATAAAAAAAAAAAAGCAGTGAATAAATTGACAGAGATTGt                                                     >  2:944977/1‑140 (MQ=255)
                                                                                gTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCt                                                 <  2:338901/140‑1 (MQ=255)
                                                                                 tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCAAACAAAAAAAAAAAATGGAGGTGGCCTGATGAGGGAAAATAAAAAAAAAAAAGGAGTGAATAAATTGACAGAGATTGTCGCTa                                                >  2:1097780/1‑140 (MQ=255)
                                                                                  ggCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAACAAAAAAAAAAAAAGGAGTTGGCCTGATGAGCGAAAGTAAAAAAAAAAAAGCAAAGAATAAATTGACAAAGATTGTCGCTaa                                               >  2:12598/1‑140 (MQ=255)
                                                                                         aaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAcc                                        <  1:1133540/140‑1 (MQ=255)
                                                                                                     caTTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCt                            <  1:1057866/140‑1 (MQ=255)
                                                                                                     caTTTTTAGTGGTAGTTTGGGGGGGTA‑ATTTTTCCCAGAAAAATACAAAAACTGGAGTGCCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTAAATAAGTTAACAGAGATTGTCGCAAACTTTACCGCCATGATTTCt                            <  2:849373/140‑1 (MQ=255)
                                                                                                           tAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGa                      <  1:30333/140‑1 (MQ=255)
                                                                                                           tAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGa                      <  1:302807/140‑1 (MQ=255)
                                                                                                            aGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGaa                     <  1:604906/140‑1 (MQ=255)
                                                                                                            aGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATAAAAAAACGGGAGTTGCCATGATGAGCGAAAGTAATAACCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGaa                     <  2:331886/140‑1 (MQ=255)
                                                                                                             gTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACGGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAAt                    <  1:1097780/140‑1 (MQ=255)
                                                                                                                gTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAAAAAAAAATGGAGTTGTCATGTTGAGGGAAAGTAAAAAACAACAAGCAGTGAATAAGTTGTGAGAGAATTTTGCTAACTTTATCCCCCTGATTTTTACCCCAATGcc                 >  2:1057866/1‑140 (MQ=255)
                                                                                                                    tttGTGGCGGTG‑AATTTTTTCAGACAAATACAAAAAATGGGGGTGGCATGATGGGCGGAAGAAATAAACAAAAAGCAGTGAAAAAATTGAAAGAGAATGTCGCTAACTTTTACGCCCTGATTTTTTTCCGAATGCCtgat             >  2:846584/1‑140 (MQ=255)
                                                                                                                     ttGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCtgatg            <  1:652681/140‑1 (MQ=255)
                                                                                                                         gggggTG‑AATTTTCCCAAAAAAATACAAAAACTGGAGTTGCCATGAGGAGCGAAAGTAAAAAGCAACGGGCGGTAAATAAGTTGAAAGAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgt        <  2:869711/137‑1 (MQ=255)
                                                                                                                          gCGGTG‑AATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtg       <  2:574137/140‑1 (MQ=255)
                                                                                                                             gTG‑AATTTTTTCCGACAAAAAAAAAAAATGGGGGTGCCATGATGGGCGGAAGGAAAAAACAAAAAGCAGGGAAAAAGTTGACAGAGGTTGTCGCTAACTTTACCGCCCTGATTTTTTCCCGAAAGCCTGATGACgtgggg    >  2:875783/1‑138 (MQ=255)
                                                                                                                               tggaTTTTTTCCGACAAAAAAAAAAAAAGGGGTTGGCATGATGAGGGAAAAGAAAAAACAACAAGCAGGGAAAAAATTGACAGAGAATGTCGCTAACTTTTTCGCCATGATTTCTACCCGAATGCCTGATGACGgggggg   >  2:85978/4‑140 (MQ=255)
                                                                                                                               g‑AATTTTTCCAGACAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa  <  1:944977/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                   
AACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGA  >  NC_000913/3206300‑3206566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑