Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,101,5070TG33.3% 57.4 / 12.1 42intergenic (+77/‑183)sodA/kdgTsuperoxide dismutase (Mn)/2‑dehydro‑3‑deoxy‑D‑gluconate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base T (5/23);  new base G (14/0);  total (19/23)
Fisher's exact test for biased strand distribution p-value = 2.20e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AGAGTTCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCATT  >  NC_000913/4101370‑4101642
                                                                                                                                         |                                                                                                                                       
agagTTCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGttt                                                                                                                                       >  1:255560/1‑140 (MQ=255)
    ttCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGttttttt                                                                                                                                   >  1:986032/1‑140 (MQ=255)
    ttCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGttttttt                                                                                                                                   >  2:428099/1‑140 (MQ=255)
    ttCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGgtttttt                                                                                                                                   >  1:949825/1‑140 (MQ=255)
              tggtgAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGGTTTTTTTTGATCaaat                                                                                                                         >  2:306924/1‑138 (MQ=255)
              tggtgAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGGTTTTTTGTGATCAAtt                                                                                                                         >  1:948572/1‑140 (MQ=255)
                 tgAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGGTTTTTTTTGGTCAAattta                                                                                                                      >  2:101416/1‑138 (MQ=255)
                   aaCTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGGTTTTTTTTGATCAATTTCaaa                                                                                                                    >  2:428557/1‑140 (MQ=255)
                       gggACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGGTTTTTTTTGGTCAAATTTAAAATaa                                                                                                                >  2:875801/1‑140 (MQ=255)
                        ggACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGGTTTTTTTTGATTAATTTTaaaaaaaa                                                                                                               >  2:116088/1‑140 (MQ=255)
                              agcagcGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGGTTTTTTTTGGTCAATTTTAaaaaaaaaaaaaa                                                                                                         >  2:661442/1‑139 (MQ=255)
                                   cGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTTTGATCAATTTCAAAATAAAAACAAAGATcc                                                                                                    >  2:371291/1‑140 (MQ=255)
                                      cACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGat                                                                                                 <  1:428099/140‑1 (MQ=255)
                                      cACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGat                                                                                                 <  2:1041819/140‑1 (MQ=255)
                                       aCGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTTTGATCAATTTTAAAATAAAAAAAAAGATCCCata                                                                                                >  2:916547/1‑140 (MQ=255)
                                        cGTTTTGCGGCGAAAAAATAATTATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGataa                                                                                               <  1:96718/140‑1 (MQ=255)
                                        cGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTGTTTTTTTGATCAATTTTAAAATAAAAAAAAAGATCCGataa                                                                                               >  2:445251/1‑140 (MQ=255)
                                               cggcgAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGGTTTTTTGTGATCAAATTCAAAAAAAAAAAAATGATCCGataaaaaaaaa                                                                                        >  1:88563/1‑140 (MQ=255)
                                                      aaaaTAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGt                                                                                 <  2:1191656/140‑1 (MQ=255)
                                                          taatCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCa                                                                             <  1:193365/140‑1 (MQ=255)
                                                                tttGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGAt                                                                       <  1:798665/140‑1 (MQ=255)
                                                                tttGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGAt                                                                       <  2:11813/140‑1 (MQ=255)
                                                                 ttGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGAtg                                                                      <  1:1142721/140‑1 (MQ=255)
                                                                 ttGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGAtg                                                                      <  1:661442/140‑1 (MQ=255)
                                                                  tGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGGTTTTTTTTGATTAATTTTAAAATAAAAAAAAAAATCCGATAAAAATAAAAAAACGGTTCaaatttttt                                                                     >  2:798665/1‑133 (MQ=255)
                                                                   gccgccTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGGTTTTTTGTGGTCAATTTTAAAATAAAAACAAAGATCCGATAAAAATAAAAAAACGTTTTAATTTAtgtg                                                                    >  2:193365/1‑140 (MQ=255)
                                                                      gccTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGtt                                                                 <  1:101416/140‑1 (MQ=255)
                                                                           ctgcAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGAt                                                            <  1:875801/140‑1 (MQ=255)
                                                                           ctgcAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGAt                                                            <  1:116088/140‑1 (MQ=255)
                                                                                   aGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTa                                                    <  2:986032/140‑1 (MQ=255)
                                                                                   aGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTa                                                    <  1:306924/140‑1 (MQ=255)
                                                                                   aGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGGTTTTTTTTGATCAAATTTAAAATAAAAACAAAGATCCGATAAAAAAAAAAAAGCGTTTTAATTGATGTGGTTTTGATTACttttt                                                    >  2:896655/1‑139 (MQ=255)
                                                                                       gTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGa                                                <  2:813827/140‑1 (MQ=255)
                                                                                                gCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGGTTTTTTTTGGTCAATTTTAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAaatgaa                                       >  1:107684/1‑140 (MQ=255)
                                                                                                 cATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATTAATTTTAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTTGTTTTTATCACTTTTATTGATTAaatgaaa                                      >  1:666714/1‑139 (MQ=255)
                                                                                                  atatCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGGTTTTTTTTGGTCAATTTTAAAAAAAAAAAAAAGATCCGATAAAAATAAAAAAGCGGTTCAATTTATGTGGGTTTGATCACTTTTATTTATTAaatgaatg                                     >  2:620501/1‑140 (MQ=255)
                                                                                                  atatCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGGTTTTTTTTGGTCAAATTTAAAAAAAAAACAAAGATCCGATAAAAATAAAAAAACGTTTCAATTGATGTGGTTTTTATCACTTTTATTTATTAaatgaatg                                     >  2:1703/1‑140 (MQ=255)
                                                                                                    atCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTc                                   <  1:384987/140‑1 (MQ=255)
                                                                                                          ttAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCtt                             <  2:948572/140‑1 (MQ=255)
                                                                                                              aaaaTGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGtt                         <  2:782507/140‑1 (MQ=255)
                                                                                                              aaaaTGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGtt                         <  1:916547/140‑1 (MQ=255)
                                                                                                                   gAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCAt                    <  1:1164196/140‑1 (MQ=255)
                                                                                                                              aaCGGCGGTGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATg         <  2:615192/140‑1 (MQ=255)
                                                                                                                                cggcggTGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCt       <  1:620501/140‑1 (MQ=255)
                                                                                                                                cggcggTGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCt       <  2:949825/140‑1 (MQ=255)
                                                                                                                                   cggTGGTTTTTTTTTGATCAATTTTAAAAAAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCa    >  2:944105/1‑140 (MQ=255)
                                                                                                                                     gtggtTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCAtt  <  1:152700/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                       
AGAGTTCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCATT  >  NC_000913/4101370‑4101642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑